RchiOBHm_Chr1g0375031

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0018879
Physical Location & Seq
Forward (+)
62615777 .. 62618274
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 519 bp
ATGGCGTCTTCTGCCAAGAGAAATCGAAAGGGAAAACGACCTGCTGTTCCGGAGGACTCTCCATTCTTCTGCTTAAGAATTGTTAATGTTGATGATATCAAGGCCGGAAAGAAGGAACTTCGGGCCGCAGCTGTGAAGAAGTATGGAGATCAAATGGGAGATCACATATTCCTCAAGGTTCCAAATTGTGGAGAACCTTGGAAAGTAAAGTTGAGAAAATCACCTAGTGGTAACCAAATGTGGTTAGAGGAGGGATGGGAAGCATTTACCAACTTTTACTTACTAGAACAAGGTGACACCATGGTGTTCAACTATGAAGGCAAACATTCCCATTTCCAAGTACGCATCATTGGTTGGGATGATATGGAAACTGACTACCCTAGACATGGAGACGAAGTCAAAGCCATGGTAATTTCCTCTGGATCCTCTGCTGATGGACAGGAAAATGAAGCTAGAGTCATTGTGATTTCATCCGGATCCTCTGCTGATGATCATGAAAATGAAGCTAGAGCAAAATAA

Protein Analysis

172

Amino Acids

19.35

Weight (kDa)

6.22

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 26 - 116 4.9e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018879)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g32413 FvH4_7g32450 FvH4_7g32450
rosa_chinensis RchiOBHm_Chr1g0375031
rosa_samantha Rh1AG398200 Rh1BG362200 Rh1CG375900 Rh1DG063300
rosa_wichuraiana Rw1G035340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 49
AccB7I CCANNNNNTGG 1 cut(s) 188
AccIII TCCGGA 2 cut(s) 49, 473
AciI CCGC 1 cut(s) 126
AclWI GGATC 4 cut(s) 417, 430, 471, 484
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 227
AfaI GTAC 1 cut(s) 342
AfiI CCNNNNNNNGG 2 cut(s) 188, 386
AflII CTTAAG 1 cut(s) 73
AgsI TTSAA 1 cut(s) 310
AleI CACNNNNGTG 1 cut(s) 302
AluBI AGCT 3 cut(s) 131, 452, 506
AluI AGCT 3 cut(s) 131, 452, 506
Alw26I GTCTC 1 cut(s) 384
AlwI GGATC 4 cut(s) 417, 430, 471, 484
Aor13HI TCCGGA 2 cut(s) 49, 473
AoxI GGCC 2 cut(s) 102, 123
ApeKI GCWGC 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 123
AsuHPI GGTGA 2 cut(s) 213, 305
BamHI GGATCC 2 cut(s) 422, 476
BbvI GCAGC 1 cut(s) 140
BccI CCATC 2 cut(s) 249, 428
BclI TGATCA 1 cut(s) 490
BcoDI GTCTC 1 cut(s) 384
BfaI CTAG 5 cut(s) 225, 284, 381, 453, 507
BfrI CTTAAG 1 cut(s) 73
BfuAI ACCTGC 1 cut(s) 49
BisI GCNGC 2 cut(s) 126, 129
BlsI GCNGC 2 cut(s) 127, 130
BmgT120I GGNCC 1 cut(s) 123
BmiI GGNNCC 3 cut(s) 180, 424, 478
BmsI GCATC 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 158
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 197, 300, 405
BsaWI WCCGGW 2 cut(s) 49, 473
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 386
BseAI TCCGGA 2 cut(s) 49, 473
BseDI CCNNGG 3 cut(s) 197, 300, 405
BseGI GGATG 3 cut(s) 260, 364, 470
BseLI CCNNNNNNNGG 2 cut(s) 188, 386
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 1 cut(s) 140
BshFI GGCC 2 cut(s) 104, 125
BsiSI CCGG 3 cut(s) 50, 105, 474
BslI CCNNNNNNNGG 2 cut(s) 188, 386
BsmAI GTCTC 1 cut(s) 384
BsmBI CGTCTC 1 cut(s) 384
BsnI GGCC 2 cut(s) 104, 125
Bsp13I TCCGGA 2 cut(s) 49, 473
Bsp143I GATC 5 cut(s) 148, 160, 422, 476, 490
Bsp19I CCATGG 2 cut(s) 300, 405
BspACI CCGC 1 cut(s) 126
BspANI GGCC 2 cut(s) 104, 125
BspEI TCCGGA 2 cut(s) 49, 473
BspHI TCATGA 1 cut(s) 493
BspLI GGNNCC 3 cut(s) 180, 424, 478
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 4 cut(s) 417, 430, 471, 484
BspTI CTTAAG 1 cut(s) 73
BssECI CCNNGG 3 cut(s) 197, 300, 405
BssMI GATC 5 cut(s) 148, 160, 422, 476, 490
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 197, 300, 405
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 73
BstDSI CCRYGG 2 cut(s) 300, 405
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 3 cut(s) 260, 364, 470
BstKTI GATC 5 cut(s) 151, 163, 425, 479, 493
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 5 cut(s) 148, 160, 422, 476, 490
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstPI GGTNACC 1 cut(s) 230
BstV1I GCAGC 1 cut(s) 140
BstX2I RGATCY 2 cut(s) 422, 476
BstYI RGATCY 2 cut(s) 422, 476
BsuRI GGCC 2 cut(s) 104, 125
BtgI CCRYGG 2 cut(s) 300, 405
BtsCI GGATG 3 cut(s) 260, 364, 470
BveI ACCTGC 1 cut(s) 49
CciI TCATGA 1 cut(s) 493
Cfr13I GGNCC 1 cut(s) 123
Csp6I GTAC 1 cut(s) 341
CviAII CATG 4 cut(s) 301, 386, 406, 494
CviJI RGCY 6 cut(s) 104, 125, 131, 404, 452, 506
CviKI_1 RGCY 6 cut(s) 104, 125, 131, 404, 452, 506
CviQI GTAC 1 cut(s) 341
DpnI GATC 5 cut(s) 150, 162, 424, 478, 492
DpnII GATC 5 cut(s) 148, 160, 422, 476, 490
DraIII CACNNNGTG 1 cut(s) 227
Eco130I CCWWGG 3 cut(s) 197, 300, 405
Eco32I GATATC 1 cut(s) 97
Eco91I GGTNACC 1 cut(s) 230
EcoO65I GGTNACC 1 cut(s) 230
EcoRV GATATC 1 cut(s) 97
EcoT14I CCWWGG 3 cut(s) 197, 300, 405
ErhI CCWWGG 3 cut(s) 197, 300, 405
Esp3I CGTCTC 1 cut(s) 384
FaeI CATG 4 cut(s) 304, 389, 409, 497
FaiI YATR 8 cut(s) 144, 167, 302, 315, 365, 387, 407, 495
FatI CATG 4 cut(s) 300, 385, 405, 493
FbaI TGATCA 1 cut(s) 490
Fnu4HI GCNGC 2 cut(s) 126, 129
FokI GGATG 3 cut(s) 267, 371, 457
Fsp4HI GCNGC 2 cut(s) 126, 129
FspBI CTAG 5 cut(s) 225, 284, 381, 453, 507
GluI GCNGC 2 cut(s) 126, 129
HaeIII GGCC 2 cut(s) 104, 125
HapII CCGG 3 cut(s) 50, 105, 474
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 304, 389, 409, 497
HinfI GANTC 2 cut(s) 56, 456
HpaII CCGG 3 cut(s) 50, 105, 474
HphI GGTGA 2 cut(s) 213, 305
Hpy188III TCNNGA 4 cut(s) 50, 420, 474, 494
HpyAV CCTTC 2 cut(s) 106, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 304, 389, 409, 497
Kpn2I TCCGGA 2 cut(s) 49, 473
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 5 cut(s) 148, 160, 422, 476, 490
LpnPI CCDG 6 cut(s) 54, 63, 118, 405, 425, 487
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 1 cut(s) 354
MaeI CTAG 5 cut(s) 225, 284, 381, 453, 507
MaeIII GTNAC 2 cut(s) 230, 293
MalI GATC 5 cut(s) 150, 162, 424, 478, 492
MboI GATC 5 cut(s) 148, 160, 422, 476, 490
MboII GAAGA 2 cut(s) 58, 148
MflI RGATCY 2 cut(s) 422, 476
MluCI AATT 3 cut(s) 78, 184, 411
MlyI GAGTC 2 cut(s) 50, 465
MnlI CCTC 7 cut(s) 46, 182, 241, 244, 427, 436, 490
MroI TCCGGA 2 cut(s) 49, 473
MseI TTAA 2 cut(s) 74, 84
MslI CAYNNNNRTG 2 cut(s) 302, 498
MspA1I CMGCKG 1 cut(s) 131
MspCI CTTAAG 1 cut(s) 73
MspI CCGG 3 cut(s) 50, 105, 474
MwoI GCNNNNNNNGC 1 cut(s) 11
NcoI CCATGG 2 cut(s) 300, 405
NdeII GATC 5 cut(s) 148, 160, 422, 476, 490
NlaIII CATG 4 cut(s) 304, 389, 409, 497
NlaIV GGNNCC 3 cut(s) 180, 424, 478
NmuCI GTSAC 1 cut(s) 293
OliI CACNNNNGTG 1 cut(s) 302
PagI TCATGA 1 cut(s) 493
PflFI GACNNNGTC 1 cut(s) 395
PflMI CCANNNNNTGG 1 cut(s) 188
PkrI GCNGC 2 cut(s) 127, 130
PleI GAGTC 2 cut(s) 50, 464
PpsI GAGTC 2 cut(s) 50, 464
PspEI GGTNACC 1 cut(s) 230
PspN4I GGNNCC 3 cut(s) 180, 424, 478
PspPI GGNCC 1 cut(s) 123
PsuI RGATCY 2 cut(s) 422, 476
PsyI GACNNNGTC 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 1 cut(s) 342
RsaNI GTAC 1 cut(s) 341
RseI CAYNNNNRTG 2 cut(s) 302, 498
SaqAI TTAA 2 cut(s) 74, 84
SatI GCNGC 2 cut(s) 126, 129
Sau3AI GATC 5 cut(s) 148, 160, 422, 476, 490
Sau96I GGNCC 1 cut(s) 123
SchI GAGTC 2 cut(s) 50, 465
SetI ASST 8 cut(s) 43, 133, 180, 199, 226, 295, 454, 508
SfaNI GCATC 1 cut(s) 354
SmiMI CAYNNNNRTG 2 cut(s) 302, 498
SmlI CTYRAG 2 cut(s) 73, 173
SmoI CTYRAG 2 cut(s) 73, 173
Sse9I AATT 3 cut(s) 78, 184, 411
SsiI CCGC 1 cut(s) 126
SspMI CTAG 5 cut(s) 225, 284, 381, 453, 507
StyI CCWWGG 3 cut(s) 197, 300, 405
TaqI TCGA 1 cut(s) 25
TasI AATT 3 cut(s) 78, 184, 411
TauI GCSGC 1 cut(s) 128
Tru1I TTAA 2 cut(s) 74, 84
Tru9I TTAA 2 cut(s) 74, 84
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 1 cut(s) 128
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 5 cut(s) 330, 459, 462, 510, 516
Tth111I GACNNNGTC 1 cut(s) 395
Van91I CCANNNNNTGG 1 cut(s) 188
Vha464I CTTAAG 1 cut(s) 73
XspI CTAG 5 cut(s) 225, 284, 381, 453, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.