RCHIOBHM_CPG0502411

DNA-directed 5'-3' RNA polymerase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Pt
OG0017067
Physical Location & Seq
Forward (+)
89573 .. 96830
7258 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15716

Sequence Viewer

Length: 6390 bp
ATGAATCAGAATTTTTCTTCTATGATTGATCGGTATAAACATCAACAACTTCGGATTGGATTAGTTTCTCCTCAACAAATAAGCGCTTGGGCACAAAAAATCCTACCTAATGGAGAGATAGTTGGGGAGGTGACAAAACCCTATACTTTTCATTACAAAACAAATAAACCTGAAAAAGACGGATTGTTTTGTGAAAGAATTTTTGGGCCTATAAAAAGTGGGATTTGTGCTTGTGGAAATTATCGAGTAATCGGAGATGAAAAAAAAGACCCGAGATTTTGTGAACAGTGTGGAGTTGAATTTGTTGATTCTCGGATACGAAGATATCAAATGGGCTACATCAAACTGGCATGCCCAGTAACCCACGTATGGTATTTGAAACGTCTTCCTAGTTATATCGCGAATCTCTTAGATAAGCCTCTTAAAGAATTAGAAGGCCTAGTATATTGTGATCCGGATGAGAGGAAACTTTCACGTCCGATTTTGAAAGGGGGAGATCCTATAGGATCCTATCCAAATTTTTCTTTTGCTAGGCCCATAGCTAAAAAACCCACTTTCTTACGATTACGAGGTTCATTCGAATACGAAATCCAATCCTGGAAATACAGCATCCCACTTTTTTTTACTACTCCAGGCTTCGATACATTTCGAAATCGAGAAATTTCTACTGGAGCAGGTGCTATCCGAGAACAATTAGCCGATCTGGATTTGAGAATTATTATAGATTATTCGCTGTTAGAATGGAAAGAATTGGGGGAAGAAGGGTCCACGGGGAATGAATGGGAAGATCGAAAGGTTGGAAGACGAAAGGATTTTTTGGTTAGACGCATGGAGTTGGCTAAGCATTTTATTCGAACAAATATAGAACCAGAATGGATGGTTTTATGTCTATTACCAGTTCTTCCGCCCGAGTTGAGACCAATCATTCAGATAGATGGGGGTAAACTAATGAGCTCGGATATTAATGAACTCTATAGAAGAGTTATTTATCGGAACAATACTCTTATCGATCTCTTAACAACAAGTAGATCTACGCCAGGAGAATTAGTAATGTGTCAGGAGAAATTGGTACAAGAAGCTGTGGATACACTTCTTGATAATGGAATCCGTGGACAACCAATGAGGGACGGTCATAATAAGGTTTACAAGTCGTTTTCAGATGTAATTGAAGGAAAAGAGGGAAGATTTCGTGAGACTCTGCTTGGCAAACGGGTCGATTATTCGGGGCGTTCTGTCATTGTTGTAGGTCCTTCACTTTCATTACATCGATGTGGATTGCCTCGCGAAATAGCAATAGAGCTTTTCCAGACATTTGTAATTCGTGGTTTAATTAGACAGCATTTTGCCTCGAATATAGGAGTGGCTAAGAGTAAAATTCGGGAAAAAGAGCCGGTTGTATGGGAAATACTTCAGGAAGTTATGCAAGGGCATCCCGTATTGCTGAATAGGGCGCCTACTCTGCATAGATTAGGCATACAGGCGTTCCAGCCCATTTTAGTAGAAGGACATGCTATTTGTTTACATCCATTAGTTTGTAAGGGATTTAACGCAGACTTTGATGGGGATCAAATGGCTGTTCATGTACCTTTATCTTTGGAGGCTCAAGCAGAGGCCCGTTTACTTATGTTTTCTCATATGAATCTCTTGTCTCCAGCTATTGGGGATCCTATTTCCGTACCAACCCAAGATATGCTTATCGGGCTCTATGTATTAACGAGCGGGAATCGCCGAGGTATTTGTGCAAATAGGTATAATCCATATAATCGCACAAATTCGAAAAATGAAAGAATTGCCGATAATAATTATAAATATACGAAAGAACCTTTTTTTTGTAATTCCTATGATGCAATTGGAGCTTATCGGCAGAAAAGAGTCAATTTAGATAGCCCCTTGTGGCTCCGGTGGCGGCTAGATCAACGTGTTATTACTTCAAGAGAAACTCCCATCGAAGTTCACTATGAGTCTTTGGGTACCTCTCATGAGATTTATGGGCACTATGTAATAGTAAGAAGTATAAAAAAAGAAGTTCTTTGTATATATGTTCGAACCACAGTTGGTCATATTTCTCTTTATCGAGAAATCGAAGAAGCTATCCAAGGGTTTTGCCGAGCCTACTCGTATGCTAGGACCACAGTTCCTGAATTTCTATGCGAATCAAGATCGAGAAAAGGAAGTTTTCCAATCACTGACTCAAACCCATTGTCGAACCCCACTCAGCGGAATAGGGAGGTACTTATGGCAGAACGGGCCAGTCTGGTTTTTCACAATAAAGTGATCGATGGAACCGCTATTAAACGACTTATTAGTAGATTAATAGATCACTTCGGAATGGCATATACATCACACATCCTAGATCAAGTAAAGACTCTGGGGTTTCGACAAGCCACTGCTACATCCATTTCATTAGGAATTGATGATCTTTTAACAATACCTTCTAAGGGGTGGCTAGTCCAAGATGCCGAACAACAAAGTTTGATTTTGGAAAAACACCATCATTATGGTAATGTACACGCGGTAGAAAAATTACGCCAATCTATTGAGATATGGTATGCTACAAGTGAATATTTGCGACAAGAAATGAATCCTAATTTTAGGATGACGGACCCTTTTAATCCAGTCCATATGATGTCTTTTTCGGGAGCTAGAGGAAATGCATCTCAAGTACACCAATTAGTAGGTATGAGAGGGTTAATGTCGGATCCACAAGGACAAATGATTGATTTACCTATTCAAAGCAATTTACGTGAAGGGCTGTCTTTAACAGAATATATCATTTCTTGTTACGGAGCCCGCAAGGGCGTTGTGGATACTGCTGTACGAACGTCGGATGCTGGATATCTTACACGCAGACTTGTTGAAGTAGTTCAACATATTGTTGTACGTAGAACAGATTGTGGCACCGTCCGAGGTATTTCTGTGAGTCCTCGAAATGGGATGATGCCGGAAAGAATTTTTATCCAAACATTGATTGGTCGTGTATTAGCAGACGATATATATATAGGACCGCGATGCATTGCCGTTCGAAATCAAGATATTGGAATTGGACTTGTCAATCGATTCATAACCTTTCAAACACAACCAATACCTATTCGAACCCCCTTTACTTGTAAGAGTACATCTTGGATCTGCCGATTATGTTATGGCCGGAGCCCTACTCATGGCGACCTGGTCGAGTTGGGGGAAGCTGTAGGCATTATTGCGGGTCAATCTATTGGAGAACCGGGCACTCAATTAACATTAAGAACTTTTCATACCGGTGGAGTATTCACAGGGGGTACTGCAGAACATGTACGAGCCCCTTCTAATGGAAAAATAAAATTCAATGAGGATTTGGTTCATCCCACACGTACACGTCATGGGCATCCTGCTTTTCTATGTTATATAAACTTGTATGTAACTATTGAAAGTGAAAATATTATACATAACGTGACTATTCCACCAAAAAGTTTGATTTTAGTGCAAAATGACCAATACGTAGAATCCGAGCAAGTGGTTGCTGAGATTCGCGCGGGAGCATACACTTTTAATTTTAAAGAGAGGGTTCGAAAACATATTTATTCTGACTCAGAGGGAGAAATGCACTGGAGTACCGATGTATATCATGCGCCAGAATTTACATATAGTAATGTACATCTTTTACCCAAAACAAGTCATTTGTGGATATTATCAGGAGGTTCGTGCAGATTCAGTGCAGTCCCCCCTTCACTCCACAAGGATCAAGATCAAACGAACGTTCATTCTCTTTCGGTTGAAGGACGATATTTTTCTAGTCTTTCAGTAAATAATGATCAAGTGAAACACAAATTCTTTGGTTTGAATCTTTCTGGTAAAAAAGAAAGCTGTATTCCTGATTATTCAGAATTGAATCGAATCATATATACGAGTCATTGTAATCTCATATTTCCTCCTATTCGCCATGATAATTTTTTATTGACAAAGAGGCGAAGAAATAGATTCATCATTCCGTTCCAATCGATTCAAGAACAAGAGAAAGAACGGATGCCCCGTCCCGATATTTCGATTGAAATACCGATAAATGGTATTTTTCGTAGAAATAGTATTCTTGCTTATTTCGACGATCCTCAATACAGAAGAAAGAGTTCAGGAATTACTAAATATGGAACTGTAGGGTTGCATTCAATCCTCAAAAAAGAGGATTTAATTGAGTACCGAGGAGTCAAAGAATTTAAGCCAAAATACCAAACGAAAGTAGATCGATTTTTTTTCATTCCTGAGGAAGTGCATATTTTACCCGAGTCTTCTTCCATAATGGTACGGAACAATAGTATCATTGGGATAGATACACGAATCACTTTAAATACAAGAAGCAGAGTAGGTGGCTTGGTCCGAATAGAGAGAAAAAAAAAAAGGATTGAACTTAAAATCTTTTCTGGAGATATCCATTTTCCTGGAGAGATGGATAAGATATTCCGACACAATGGCATCTTGATACCGCCAGGAACAAATTCTAAGGAATCAAAAAAGCGGAACAATTGGATTTATGTCCAATGGATCACACCCACCAAGAAAAAGTATTTTGTTTTGGTTCGACCCGTAATCATATATGAAATTGCGGACGGTATAAATTTAGCAACACTTTTCCCCCAGGATCCATTGCGGGAAAGGGATAATCTAGAACTTCGAGTTGTAAATTATATACTTTATGGAAATGGCAAACCAATTCGGGGAATTTCCGGCACAAGTATTCAATTAGTTCGGACCTGTTTAATCTTGAACTGGGACAAAAACAAAAAAAGTTCTTCTATCGAAGAGGCCCACGCTTCCTTTGTTGAAGTAAGTGCGAATGGTCTGATTCAAGATTTCCTCAGAATCAACTTAGTGAAATCCCATACTTCGTATATCCGAAAAAGGAATGATCCTTTAGGTTCAGGATTGATCTCTGATAATAGGTCGGATCGTACCAATATCAATCCATTTTATTCTATTTATTCCAAGGAAAGAATTCAACAATCGCTTAGACAAAATCAAGGAACTTTTCGTACGTTGTTGAATAGAAATAAGGAATCCCAATCTTTGATAATTTTGTCATCATATAATTGTTTTCAAATGAGTCCATTCAACGATGTAAAATATTACGATGGGATAAAAGAATCAATTAAAAGAGATAGGGATTCCCTAATTCAAATTACAAATTTGTTAGGCCCCTTAGGAACAGCCTCTCAAATTGATCTTTTTTATTCGTTTTACCATTTACTAACTCACAATCATATTTCGGTAACTAAATATTTCTATTTGCAACTCGACAATTTAAAACAGACTTTTCAAGTATTTAAGTATTATTTAATGGATGAAAACGGGAGAATTTCGAATTCCGATCCGTGCAGTAGCATCCTTTTGAATCCATTTAACTTGAATTGGCATTTTCTCGACCATAATTATTGTGAGGAAACATCCACAATAATTAGTCTCGGGCAGTTTATTTGTGAAAATCTATGTATAGCCAAAAAAGGATCGCCCCTAAAATCGGGTCAAGTTATAGTCGTTCAACTTGACTCTTTAGTAATAAGATCGGCGAAGCCTTATTTAGCTACTCCAGGAGCAACTGTTCATGGACATTATGGAGAAATCCTTTCCGAAGGAGATACATTAGTTACATTTATATATGAAAAATCTCGATCTGGTGATATAACGCAAGGTCTTCCAAAAGTAGAACAGGTTTTAGAGGTACGTTCGATTGATTCAATATCGATGAACCTAGAAAAGAGAGTTGAGGGTTGGAATGAGTGTATAACAAGAATTCTTGGAATTCCTTGGGGATTCTTGATTGGCGCTGAGCTAACTATAGCGCAAAGTCGTATCTCTTTGGTTAATAAGATCCAAAAAGTTTATAGATCCCAGGGGGTACAGATCCATAATAGGCATATCGAAATCATTGTACGTCAAATAACATCAAAAGTGTTGGTTTCAGAAGATGGAATGTCTAATGTTTTTTCACCCGGAGAACTAATTGGATTGCTGCGAGCTGAACGAACGGGGCGCGCTTTAGAAGAAGCGATTTGTTACCGAGCCATATTATTAGGAATAACGAAAGCATCTCTAAATACTCAAAGTTTCATATCCGAAGCAAGTTTTCAAGAAACTGCTCGAGTTTTAGCAAAAGCTGCCCTCCGGGGTCGTATCGATTGGTTGAAAGGTCTGAAAGAGAACGTTGTTCTAGGAGGGATGATACCCGTTGGTACCGGATTCAAAGGATTCGTGCCCCGTTCAAGGCAACATAACAACATTTCTTTGGAAACCAAAAATAAGAGTTTATTCGAGGGGGAAATGAGAGATATTTTGGTCCACCACAGAGAATTATTTGATTTTTGCATTTCAAAAAATTTACATGATACATCAGAACAATCTTTTTTCGGATTTAATGATTCCTAA

Protein Analysis

2129

Amino Acids

242.9

Weight (kDa)

9.16

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 15 - 139 1.7e-15 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_1 PF04997 282 - 400 4.4e-30 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 402 - 449 8.3e-07 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_2 PF00623 462 - 542 1.3e-25 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 547 - 807 1.3e-13 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4 PF05000 838 - 902 2.5e-13 RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5 PF04998 917 - 1110 2.5e-39 RNA polymerase Rpb1, domain 5
RNA_pol_Rpb1_5 PF04998 1933 - 2020 2.9e-07 RNA polymerase Rpb1, domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1806
AarI CACCTGC 1 cut(s) 665
Acc36I ACCTGC 1 cut(s) 665
Acc65I GGTACC 2 cut(s) 1970, 6197
AccB1I GGYRCC 4 cut(s) 1450, 1970, 2897, 6197
AccB7I CCANNNNNTGG 1 cut(s) 1658
AccBSI CCGCTC 1 cut(s) 1719
AccII CGCG 7 cut(s) 401, 1284, 2513, 3007, 3507, 3509, 6000
AccIII TCCGGA 1 cut(s) 454
AclI AACGTT 2 cut(s) 3732, 6168
AcoI YGGCCR 1 cut(s) 3142
AcuI CTGAAG 1 cut(s) 1394
AcyI GRCGYC 1 cut(s) 1451
AfeI AGCGCT 1 cut(s) 85
AflIII ACRYGT 4 cut(s) 1918, 3286, 3344, 3350
AgeI ACCGGT 1 cut(s) 3254
AjiI CACGTC 2 cut(s) 476, 3353
AjnI CCWGG 9 cut(s) 596, 631, 1036, 3165, 4408, 4456, 4605, 5554, 5856
AjuI GAANNNNNNNTTGG 2 cut(s) 186, 218
AloI GAACNNNNNNTCC 2 cut(s) 5550, 5582
Alw21I GWGCWC 1 cut(s) 956
Alw26I GTCTC 4 cut(s) 910, 1187, 1653, 5432
AlwNI CAGNNNCTG 1 cut(s) 2138
Ama87I CYCGRG 5 cut(s) 271, 908, 4253, 5429, 6105
Aor13HI TCCGGA 1 cut(s) 454
Aor51HI AGCGCT 1 cut(s) 85
AoxI GGCC 8 cut(s) 206, 436, 533, 1611, 2247, 3142, 4773, 5161
ApeKI GCWGC 2 cut(s) 5977, 6122
ArsI GACNNNNNNTTYG 4 cut(s) 1497, 1529, 2186, 2218
AseI ATTAAT 2 cut(s) 963, 2312
AsiGI ACCGGT 1 cut(s) 3254
Asp700I GAANNNNTTC 5 cut(s) 1407, 2862, 4099, 4465, 4962
Asp718I GGTACC 2 cut(s) 1970, 6197
AspLEI GCGC 8 cut(s) 86, 1453, 3509, 3607, 5792, 5809, 6000, 6002
AsuC2I CCSGG 3 cut(s) 3222, 5958, 6131
AsuHPI GGTGA 3 cut(s) 142, 5654, 5946
AsuII TTCGAA 9 cut(s) 579, 649, 853, 1775, 2044, 3022, 3091, 3544, 5327
AvaI CYCGRG 5 cut(s) 271, 908, 4253, 5429, 6105
AvaII GGWCC 8 cut(s) 765, 1247, 2127, 2602, 3002, 4345, 4719, 6301
AxyI CCTNAGG 2 cut(s) 4233, 5167
BaeGI GKGCMC 4 cut(s) 94, 1995, 3227, 6222
BaeI ACNNNNGTAYC 8 cut(s) 2799, 2832, 3571, 3604, 4270, 4303, 5595, 5628
BamHI GGATCC 4 cut(s) 506, 1663, 2698, 4609
BanI GGYRCC 4 cut(s) 1450, 1970, 2897, 6197
BanII GRGCYC 5 cut(s) 956, 1704, 2791, 3152, 3298
BbsI GAAGAC 4 cut(s) 377, 808, 4251, 5651
Bbv12I GWGCWC 1 cut(s) 956
BbvI GCAGC 2 cut(s) 5964, 6109
BccI CCATC 9 cut(s) 871, 929, 1553, 1952, 2273, 2499, 4411, 5093, 5927
BceAI ACGGC 1 cut(s) 3002
BcgI CGANNNNNNTGC 4 cut(s) 833, 867, 1195, 1229
BciT130I CCWGG 9 cut(s) 598, 633, 1038, 3167, 4410, 4458, 4607, 5556, 5858
BciVI GTATCC 3 cut(s) 309, 1078, 2800
BclI TGATCA 1 cut(s) 3787
BcnI CCSGG 3 cut(s) 3222, 5958, 6131
BcoDI GTCTC 4 cut(s) 910, 1187, 1653, 5432
BfmI CTRYAG 6 cut(s) 501, 973, 3186, 3279, 4125, 5802
BfoI RGCGCY 3 cut(s) 87, 1454, 5793
BfuAI ACCTGC 1 cut(s) 665
BfuI GTATCC 3 cut(s) 309, 1078, 2800
BglII AGATCT 1 cut(s) 1028
BisI GCNGC 3 cut(s) 1907, 5978, 6123
BlpI GCTNAGC 2 cut(s) 840, 5793
BlsI GCNGC 3 cut(s) 1908, 5979, 6124
Bme18I GGWCC 8 cut(s) 765, 1247, 2127, 2602, 3002, 4345, 4719, 6301
BmeT110I CYCGRG 5 cut(s) 271, 908, 4253, 5429, 6105
BmgBI CACGTC 2 cut(s) 476, 3353
BmrI ACTGGG 2 cut(s) 350, 4747
BmuI ACTGGG 2 cut(s) 350, 4747
BpiI GAAGAC 4 cut(s) 377, 808, 4251, 5651
BplI GAGNNNNNCTC 2 cut(s) 3139, 3171
BpmI CTGGAG 7 cut(s) 615, 690, 1635, 3604, 4413, 4431, 5538
Bpu1102I GCTNAGC 2 cut(s) 840, 5793
Bpu14I TTCGAA 9 cut(s) 579, 649, 853, 1775, 2044, 3022, 3091, 3544, 5327
BpuEI CTTGAG 2 cut(s) 1587, 2643
BpuMI CCSGG 3 cut(s) 3222, 5958, 6131
Bsa29I ATCGAT 8 cut(s) 1008, 1267, 2277, 3055, 3974, 4216, 5708, 6141
BsaAI YACGTR 5 cut(s) 367, 2744, 2882, 3347, 3475
BsaBI GATNNNNATC 3 cut(s) 1563, 3597, 3720
BsaHI GRCGYC 1 cut(s) 1451
BsaI GGTCTC 1 cut(s) 910
BsaWI WCCGGW 4 cut(s) 454, 1899, 3254, 6200
BsaXI ACNNNNNCTCC 6 cut(s) 105, 135, 1032, 1062, 5550, 5580
Bse118I RCCGGY 2 cut(s) 1390, 3254
Bse1I ACTGG 8 cut(s) 351, 356, 673, 896, 2250, 2615, 3587, 4742
Bse21I CCTNAGG 2 cut(s) 4233, 5167
Bse3DI GCAATG 2 cut(s) 3012, 4613
Bse8I GATNNNNATC 3 cut(s) 1563, 3597, 3720
BseAI TCCGGA 1 cut(s) 454
BseBI CCWGG 9 cut(s) 598, 633, 1038, 3167, 4410, 4458, 4607, 5556, 5858
BseCI ATCGAT 8 cut(s) 1008, 1267, 2277, 3055, 3974, 4216, 5708, 6141
BseJI GATNNNNATC 3 cut(s) 1563, 3597, 3720
BseMI GCAATG 2 cut(s) 3012, 4613
BseMII CTCAG 6 cut(s) 2228, 3489, 3579, 4224, 4840, 5784
BseNI ACTGG 8 cut(s) 351, 356, 673, 896, 2250, 2615, 3587, 4742
BsePI GCGCGC 1 cut(s) 5998
BseRI GAGGAG 2 cut(s) 60, 4188
BseSI GKGCMC 4 cut(s) 94, 1995, 3227, 6222
BseXI GCAGC 2 cut(s) 5964, 6109
BsgI GTGCAG 3 cut(s) 3700, 3711, 5362
Bsh1236I CGCG 7 cut(s) 401, 1284, 2513, 3007, 3507, 3509, 6000
BshFI GGCC 8 cut(s) 208, 438, 535, 1613, 2249, 3144, 4775, 5163
BshNI GGYRCC 4 cut(s) 1450, 1970, 2897, 6197
BshTI ACCGGT 1 cut(s) 3254
BshVI ATCGAT 8 cut(s) 1008, 1267, 2277, 3055, 3974, 4216, 5708, 6141
BsiHKAI GWGCWC 1 cut(s) 956
BsiHKCI CYCGRG 5 cut(s) 271, 908, 4253, 5429, 6105
BsiWI CGTACG 1 cut(s) 5000
BslFI GGGAC 4 cut(s) 1139, 3680, 3993, 4754
BsmAI GTCTC 4 cut(s) 910, 1187, 1653, 5432
BsmFI GGGAC 4 cut(s) 1139, 3680, 3993, 4754
BsmI GAATGC 1 cut(s) 4135
BsnI GGCC 8 cut(s) 208, 438, 535, 1613, 2249, 3144, 4775, 5163
Bso31I GGTCTC 1 cut(s) 910
BsoBI CYCGRG 5 cut(s) 271, 908, 4253, 5429, 6105
Bsp119I TTCGAA 9 cut(s) 579, 649, 853, 1775, 2044, 3022, 3091, 3544, 5327
Bsp1286I GDGCHC 9 cut(s) 94, 956, 1704, 1995, 2791, 3152, 3227, 3298, 6222
Bsp13I TCCGGA 1 cut(s) 454
Bsp1407I TGTACA 2 cut(s) 2506, 3628
Bsp1720I GCTNAGC 2 cut(s) 840, 5793
Bsp68I TCGCGA 2 cut(s) 401, 1284
BspANI GGCC 8 cut(s) 208, 438, 535, 1613, 2249, 3144, 4775, 5163
BspCNI CTCAG 6 cut(s) 2227, 3490, 3578, 4225, 4839, 5785
BspDI ATCGAT 8 cut(s) 1008, 1267, 2277, 3055, 3974, 4216, 5708, 6141
BspEI TCCGGA 1 cut(s) 454
BspFNI CGCG 7 cut(s) 401, 1284, 2513, 3007, 3507, 3509, 6000
BspHI TCATGA 1 cut(s) 1978
BspMAI CTGCAG 1 cut(s) 3283
BspMI ACCTGC 1 cut(s) 665
BspT104I TTCGAA 9 cut(s) 579, 649, 853, 1775, 2044, 3022, 3091, 3544, 5327
BspT107I GGYRCC 4 cut(s) 1450, 1970, 2897, 6197
BspTNI GGTCTC 1 cut(s) 910
BsrBI CCGCTC 1 cut(s) 1719
BsrDI GCAATG 2 cut(s) 3012, 4613
BsrFI RCCGGY 2 cut(s) 1390, 3254
BsrGI TGTACA 2 cut(s) 2506, 3628
BsrI ACTGG 8 cut(s) 351, 356, 673, 896, 2250, 2615, 3587, 4742
BssAI RCCGGY 2 cut(s) 1390, 3254
BssHII GCGCGC 1 cut(s) 5998
BssNI GRCGYC 1 cut(s) 1451
BssT1I CCWWGG 3 cut(s) 2095, 4953, 5771
Bst2UI CCWGG 9 cut(s) 598, 633, 1038, 3167, 4410, 4458, 4607, 5556, 5858
Bst4CI ACNGT 8 cut(s) 288, 1130, 2053, 2134, 2902, 4126, 4580, 5566
Bst6I CTCTTC 2 cut(s) 973, 4764
BstACI GRCGYC 1 cut(s) 1451
BstAPI GCANNNNNTGC 1 cut(s) 6122
BstAUI TGTACA 2 cut(s) 2506, 3628
BstBAI YACGTR 5 cut(s) 367, 2744, 2882, 3347, 3475
BstBI TTCGAA 9 cut(s) 579, 649, 853, 1775, 2044, 3022, 3091, 3544, 5327
BstC8I GCNNGC 4 cut(s) 352, 2791, 5982, 6000
BstDSI CCRYGG 2 cut(s) 768, 1108
BstFNI CGCG 7 cut(s) 401, 1284, 2513, 3007, 3507, 3509, 6000
BstH2I RGCGCY 3 cut(s) 87, 1454, 5793
BstHHI GCGC 8 cut(s) 86, 1453, 3509, 3607, 5792, 5809, 6000, 6002
BstMAI GTCTC 4 cut(s) 910, 1187, 1653, 5432
BstMWI GCNNNNNNNGC 7 cut(s) 1459, 1699, 1725, 1853, 1903, 2246, 6122
BstNI CCWGG 9 cut(s) 598, 633, 1038, 3167, 4410, 4458, 4607, 5556, 5858
BstNSI RCATGY 3 cut(s) 354, 1511, 3290
BstSFI CTRYAG 6 cut(s) 501, 973, 3186, 3279, 4125, 5802
BstSLI GKGCMC 4 cut(s) 94, 1995, 3227, 6222
BstSNI TACGTA 2 cut(s) 2882, 3475
BstUI CGCG 7 cut(s) 401, 1284, 2513, 3007, 3507, 3509, 6000
BstV1I GCAGC 2 cut(s) 5964, 6109
BstV2I GAAGAC 4 cut(s) 377, 808, 4251, 5651
BstXI CCANNNNNNTGG 2 cut(s) 2498, 4409
Bsu15I ATCGAT 8 cut(s) 1008, 1267, 2277, 3055, 3974, 4216, 5708, 6141
Bsu36I CCTNAGG 2 cut(s) 4233, 5167
BsuI GTATCC 3 cut(s) 309, 1078, 2800
BsuRI GGCC 8 cut(s) 208, 438, 535, 1613, 2249, 3144, 4775, 5163
BsuTUI ATCGAT 8 cut(s) 1008, 1267, 2277, 3055, 3974, 4216, 5708, 6141
BtgI CCRYGG 2 cut(s) 768, 1108
BtgZI GCGATG 1 cut(s) 3022
BtrI CACGTC 2 cut(s) 476, 3353
BtsI GCAGTG 1 cut(s) 2385
BtsIMutI CAGTG 5 cut(s) 293, 2184, 2385, 3580, 3694
BtuMI TCGCGA 2 cut(s) 401, 1284
BveI ACCTGC 1 cut(s) 665
Cac8I GCNNGC 4 cut(s) 352, 2791, 5982, 6000
CaiI CAGNNNCTG 1 cut(s) 2138
CciI TCATGA 1 cut(s) 1978
CfoI GCGC 8 cut(s) 86, 1453, 3509, 3607, 5792, 5809, 6000, 6002
Cfr10I RCCGGY 2 cut(s) 1390, 3254
ClaI ATCGAT 8 cut(s) 1008, 1267, 2277, 3055, 3974, 4216, 5708, 6141
CseI GACGC 1 cut(s) 834
CsiI ACCWGGT 1 cut(s) 3165
CspAI ACCGGT 1 cut(s) 3254
DinI GGCGCC 1 cut(s) 1452
DraI TTTAAA 3 cut(s) 3532, 4317, 5271
EaeI YGGCCR 1 cut(s) 3142
Eam1104I CTCTTC 2 cut(s) 973, 4764
EarI CTCTTC 2 cut(s) 973, 4764
EciI GGCGGA 1 cut(s) 894
Ecl136II GAGCTC 1 cut(s) 954
Eco105I TACGTA 2 cut(s) 2882, 3475
Eco130I CCWWGG 3 cut(s) 2095, 4953, 5771
Eco147I AGGCCT 1 cut(s) 438
Eco24I GRGCYC 5 cut(s) 956, 1704, 2791, 3152, 3298
Eco31I GGTCTC 1 cut(s) 910
Eco32I GATATC 3 cut(s) 326, 2837, 4399
Eco47I GGWCC 8 cut(s) 765, 1247, 2127, 2602, 3002, 4345, 4719, 6301
Eco47III AGCGCT 1 cut(s) 85
Eco53kI GAGCTC 1 cut(s) 954
Eco57I CTGAAG 1 cut(s) 1394
Eco81I CCTNAGG 2 cut(s) 4233, 5167
Eco88I CYCGRG 5 cut(s) 271, 908, 4253, 5429, 6105
EcoICRI GAGCTC 1 cut(s) 954
EcoO109I RGGNCCY 2 cut(s) 1247, 5162
EcoRI GAATTC 4 cut(s) 4962, 5329, 5757, 5766
EcoRII CCWGG 9 cut(s) 596, 631, 1036, 3165, 4408, 4456, 4605, 5554, 5856
EcoRV GATATC 3 cut(s) 326, 2837, 4399
EcoT14I CCWWGG 3 cut(s) 2095, 4953, 5771
EcoT22I ATGCAT 2 cut(s) 2656, 3014
EcoT38I GRGCYC 5 cut(s) 956, 1704, 2791, 3152, 3298
EgeI GGCGCC 1 cut(s) 1452
EheI GGCGCC 1 cut(s) 1452
ErhI CCWWGG 3 cut(s) 2095, 4953, 5771
FalI AAGNNNNNCTT 4 cut(s) 1677, 1709, 2013, 2045
FaqI GGGAC 4 cut(s) 1139, 3680, 3993, 4754
FauI CCCGC 5 cut(s) 1712, 2798, 3193, 3502, 4611
FauNDI CATATG 2 cut(s) 1635, 2622
FbaI TGATCA 1 cut(s) 3787
Fnu4HI GCNGC 3 cut(s) 1907, 5978, 6123
FriOI GRGCYC 5 cut(s) 956, 1704, 2791, 3152, 3298
Fsp4HI GCNGC 3 cut(s) 1907, 5978, 6123
GlaI GCGC 8 cut(s) 85, 1452, 3508, 3606, 5791, 5808, 5999, 6001
GluI GCNGC 3 cut(s) 1907, 5978, 6123
GsuI CTGGAG 7 cut(s) 615, 690, 1635, 3604, 4413, 4431, 5538
HaeII RGCGCY 3 cut(s) 87, 1454, 5793
HaeIII GGCC 8 cut(s) 208, 438, 535, 1613, 2249, 3144, 4775, 5163
HgaI GACGC 1 cut(s) 834
HhaI GCGC 8 cut(s) 86, 1453, 3509, 3607, 5792, 5809, 6000, 6002
Hin1I GRCGYC 1 cut(s) 1451
Hin6I GCGC 8 cut(s) 84, 1451, 3507, 3605, 5790, 5807, 5998, 6000
HinP1I GCGC 8 cut(s) 84, 1451, 3507, 3605, 5790, 5807, 5998, 6000
HphI GGTGA 3 cut(s) 142, 5654, 5946
Hpy99I CGWCG 2 cut(s) 2827, 4079
HpyCH4III ACNGT 8 cut(s) 288, 1130, 2053, 2134, 2902, 4126, 4580, 5566
HpyF10VI GCNNNNNNNGC 7 cut(s) 1459, 1699, 1725, 1853, 1903, 2246, 6122
Hsp92I GRCGYC 1 cut(s) 1451
HspAI GCGC 8 cut(s) 84, 1451, 3507, 3605, 5790, 5807, 5998, 6000
KasI GGCGCC 1 cut(s) 1450
Kpn2I TCCGGA 1 cut(s) 454
KpnI GGTACC 2 cut(s) 1974, 6201
Ksp22I TGATCA 1 cut(s) 3787
LmnI GCTCC 8 cut(s) 671, 1853, 1902, 2639, 2786, 3147, 3512, 5558
Lsp1109I GCAGC 2 cut(s) 5964, 6109
MabI ACCWGGT 1 cut(s) 3165
MaeIII GTNAC 8 cut(s) 130, 358, 2780, 3394, 3427, 5236, 5611, 6020
MbiI CCGCTC 1 cut(s) 1719
MfeI CAATTG 2 cut(s) 1848, 4492
MhlI GDGCHC 9 cut(s) 94, 956, 1704, 1995, 2791, 3152, 3227, 3298, 6222
Mly113I GGCGCC 1 cut(s) 1451
MmeI TCCRAC 6 cut(s) 778, 2676, 2805, 4456, 4893, 5717
Mph1103I ATGCAT 2 cut(s) 2656, 3014
MroI TCCGGA 1 cut(s) 454
MroXI GAANNNNTTC 5 cut(s) 1407, 2862, 4099, 4465, 4962
MslI CAYNNNNRTG 3 cut(s) 1269, 2496, 3255
MspA1I CMGCKG 1 cut(s) 2218
MunI CAATTG 2 cut(s) 1848, 4492
Mva1269I GAATGC 1 cut(s) 4135
MvaI CCWGG 9 cut(s) 598, 633, 1038, 3167, 4410, 4458, 4607, 5556, 5858
MvnI CGCG 7 cut(s) 401, 1284, 2513, 3007, 3507, 3509, 6000
MwoI GCNNNNNNNGC 7 cut(s) 1459, 1699, 1725, 1853, 1903, 2246, 6122
NarI GGCGCC 1 cut(s) 1451
NciI CCSGG 3 cut(s) 3222, 5958, 6131
NdeI CATATG 2 cut(s) 1635, 2622
NmeAIII GCCGAG 2 cut(s) 1754, 2132
NmuCI GTSAC 2 cut(s) 130, 3427
NruI TCGCGA 2 cut(s) 401, 1284
NsiI ATGCAT 2 cut(s) 2656, 3014
NspI RCATGY 3 cut(s) 354, 1511, 3290
NspV TTCGAA 9 cut(s) 579, 649, 853, 1775, 2044, 3022, 3091, 3544, 5327
PaeI GCATGC 1 cut(s) 354
PaeR7I CTCGAG 1 cut(s) 6105
PagI TCATGA 1 cut(s) 1978
PaqCI CACCTGC 1 cut(s) 665
PasI CCCWGGG 1 cut(s) 5857
PauI GCGCGC 1 cut(s) 5998
PceI AGGCCT 1 cut(s) 438
PciI ACATGT 1 cut(s) 3286
PcsI WCGNNNNNNNCGW 2 cut(s) 3480, 4003
PctI GAATGC 1 cut(s) 4135
PdmI GAANNNNTTC 5 cut(s) 1407, 2862, 4099, 4465, 4962
Pfl23II CGTACG 1 cut(s) 5000
PflFI GACNNNGTC 1 cut(s) 3167
PflMI CCANNNNNTGG 1 cut(s) 1658
PfoI TCCNGGA 3 cut(s) 596, 4408, 5554
PinAI ACCGGT 1 cut(s) 3254
PkrI GCNGC 3 cut(s) 1908, 5979, 6124
PluTI GGCGCC 1 cut(s) 1454
Ppu21I YACGTR 5 cut(s) 367, 2744, 2882, 3347, 3475
PpuMI RGGWCCY 1 cut(s) 1247
PscI ACATGT 1 cut(s) 3286
PshBI ATTAAT 2 cut(s) 963, 2312
PsiI TTATAA 1 cut(s) 1806
Psp124BI GAGCTC 1 cut(s) 956
Psp1406I AACGTT 2 cut(s) 3732, 6168
Psp5II RGGWCCY 1 cut(s) 1247
Psp6I CCWGG 9 cut(s) 596, 631, 1036, 3165, 4408, 4456, 4605, 5554, 5856
PspGI CCWGG 9 cut(s) 596, 631, 1036, 3165, 4408, 4456, 4605, 5554, 5856
PspLI CGTACG 1 cut(s) 5000
PspPPI RGGWCCY 1 cut(s) 1247
PspXI VCTCGAGB 1 cut(s) 6105
PsrI GAACNNNNNNTAC 6 cut(s) 3085, 3117, 3235, 3267, 4984, 5016
PstI CTGCAG 1 cut(s) 3283
PstNI CAGNNNCTG 1 cut(s) 2138
PsyI GACNNNGTC 1 cut(s) 3167
PteI GCGCGC 1 cut(s) 5998
RruI TCGCGA 2 cut(s) 401, 1284
RseI CAYNNNNRTG 3 cut(s) 1269, 2496, 3255
SacI GAGCTC 1 cut(s) 956
SatI GCNGC 3 cut(s) 1907, 5978, 6123
SduI GDGCHC 9 cut(s) 94, 956, 1704, 1995, 2791, 3152, 3227, 3298, 6222
SexAI ACCWGGT 1 cut(s) 3165
SfcI CTRYAG 6 cut(s) 501, 973, 3186, 3279, 4125, 5802
SfoI GGCGCC 1 cut(s) 1452
Sfr274I CTCGAG 1 cut(s) 6105
SfuI TTCGAA 9 cut(s) 579, 649, 853, 1775, 2044, 3022, 3091, 3544, 5327
SinI GGWCC 8 cut(s) 765, 1247, 2127, 2602, 3002, 4345, 4719, 6301
SlaI CTCGAG 1 cut(s) 6105
SmiMI CAYNNNNRTG 3 cut(s) 1269, 2496, 3255
SmlI CTYRAG 3 cut(s) 1602, 2658, 6105
SmoI CTYRAG 3 cut(s) 1602, 2658, 6105
SnaBI TACGTA 2 cut(s) 2882, 3475
SphI GCATGC 1 cut(s) 354
SseBI AGGCCT 1 cut(s) 438
SspDI GGCGCC 1 cut(s) 1450
SspI AATATT 4 cut(s) 2564, 3415, 5093, 5246
SstI GAGCTC 1 cut(s) 956
StuI AGGCCT 1 cut(s) 438
StyI CCWWGG 3 cut(s) 2095, 4953, 5771
TaaI ACNGT 8 cut(s) 288, 1130, 2053, 2134, 2902, 4126, 4580, 5566
TatI WGTACW 4 cut(s) 2506, 2662, 3113, 3628
TauI GCSGC 1 cut(s) 1909
TscAI CASTG 5 cut(s) 293, 2191, 2392, 3587, 3694
TseFI GTSAC 2 cut(s) 130, 3427
TseI GCWGC 2 cut(s) 5977, 6122
Tsp45I GTSAC 2 cut(s) 130, 3427
TspGWI ACGGA 9 cut(s) 195, 1097, 1663, 2615, 2799, 3954, 4012, 4291, 5328
TspRI CASTG 5 cut(s) 293, 2191, 2392, 3587, 3694
Tth111I GACNNNGTC 1 cut(s) 3167
Van91I CCANNNNNTGG 1 cut(s) 1658
VpaK11BI GGWCC 8 cut(s) 765, 1247, 2127, 2602, 3002, 4345, 4719, 6301
VspI ATTAAT 2 cut(s) 963, 2312
XbaI TCTAGA 1 cut(s) 4633
XceI RCATGY 3 cut(s) 354, 1511, 3290
XcmI CCANNNNNNNNNTGG 1 cut(s) 2966
XhoI CTCGAG 1 cut(s) 6105
XmnI GAANNNNTTC 5 cut(s) 1407, 2862, 4099, 4465, 4962
Zsp2I ATGCAT 2 cut(s) 2656, 3014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.