RchiOBHm_Chr1g0323471

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0022629
Physical Location & Seq
Forward (+)
10951605 .. 10951988
384 bp
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UTR
Exon/CDS
Intron
PRQ55338

Sequence Viewer

Length: 384 bp
ATGCTAGTAGTGCAAGGTCAGCTTATTGTGCGGTTTGATGATACAAATCCTGCTAAAGAAAGCAATGAGTTTGTGGAAAATTTTCTCAAAGATATTGACACTTTGGGTATCAAGTATGAGGAGGTTACCTATACTTTAGATTACTTCTCTAAGTTGATGGATATGACCAAGGAGCTGATTATCCAGGGTAAAGCATATGTCGATGATACACCTAGAGAAGAGACGCAAAAACAACAGATAGATGGGATTGAGTCAAAGTGTAGAAACCAGAGCCAAGAGGAAAATTTGAAACTGCGGGGGGAGATGACAGCAGGTTCAGAGAGGGGTCTGCAGTGTTGTGTTAGAGGGAAGCTGGCCATGCAGGACCCTAATAAATCGCTCTGA

Protein Analysis

127

Amino Acids

14.57

Weight (kDa)

4.76

Isoelectric Point (pI)

23.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c PF00749 5 - 123 4.3e-29 tRNA synthetases class I (E and Q), catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022629)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0323471
rosa_multiflora Rmu_sc0019211.1_g000002
rosa_samantha Rh1CG057800 Rh1DG061600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 302
AciI CCGC 2 cut(s) 31, 295
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 2 cut(s) 79, 283
AgsI TTSAA 1 cut(s) 289
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 3 cut(s) 22, 175, 352
AluI AGCT 3 cut(s) 22, 175, 352
Alw26I GTCTC 1 cut(s) 215
AoxI GGCC 1 cut(s) 354
ApoI RAATTY 2 cut(s) 79, 283
Asp700I GAANNNNTTC 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 364
AvaII GGWCC 1 cut(s) 364
BalI TGGCCA 1 cut(s) 356
BccI CCATC 2 cut(s) 151, 236
BciT130I CCWGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 215
BfaI CTAG 2 cut(s) 5, 213
BfmI CTRYAG 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 302
Bme1390I CCNGG 1 cut(s) 185
Bme18I GGWCC 1 cut(s) 364
BmgT120I GGNCC 1 cut(s) 364
BmiI GGNNCC 1 cut(s) 366
BmrFI CCNGG 1 cut(s) 185
BsaBI GATNNNNATC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 168, 184
Bse3DI GCAATG 1 cut(s) 70
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 2 cut(s) 168, 184
BseJI GATNNNNATC 1 cut(s) 45
BseMI GCAATG 1 cut(s) 70
BseRI GAGGAG 1 cut(s) 134
BshFI GGCC 1 cut(s) 356
BsmAI GTCTC 1 cut(s) 215
BsmBI CGTCTC 1 cut(s) 215
BsnI GGCC 1 cut(s) 356
BspACI CCGC 2 cut(s) 31, 295
BspANI GGCC 1 cut(s) 356
BspLI GGNNCC 1 cut(s) 366
BspMAI CTGCAG 1 cut(s) 333
BspMI ACCTGC 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 70
BssECI CCNNGG 2 cut(s) 168, 184
BssT1I CCWWGG 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 185
Bst6I CTCTTC 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 1 cut(s) 150
BstEII GGTNACC 1 cut(s) 124
BstMAI GTCTC 1 cut(s) 215
BstMWI GCNNNNNNNGC 4 cut(s) 10, 19, 28, 358
BstNI CCWGG 1 cut(s) 185
BstPI GGTNACC 1 cut(s) 124
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 329
BsuRI GGCC 1 cut(s) 356
BtsI GCAGTG 1 cut(s) 338
BtsIMutI CAGTG 1 cut(s) 338
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 1 cut(s) 354
Cfr13I GGNCC 1 cut(s) 364
CseI GACGC 1 cut(s) 232
CviAII CATG 1 cut(s) 358
CviJI RGCY 5 cut(s) 22, 175, 273, 352, 356
CviKI_1 RGCY 5 cut(s) 22, 175, 273, 352, 356
DdeI CTNAG 1 cut(s) 150
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 364
Eco91I GGTNACC 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 168
Esp3I CGTCTC 1 cut(s) 215
FaeI CATG 1 cut(s) 361
FaiI YATR 6 cut(s) 117, 132, 164, 196, 198, 359
FalI AAGNNNNNCTT 1 cut(s) 38
FatI CATG 1 cut(s) 357
FauI CCCGC 1 cut(s) 288
FauNDI CATATG 1 cut(s) 196
FspBI CTAG 2 cut(s) 5, 213
HaeIII GGCC 1 cut(s) 356
HgaI GACGC 1 cut(s) 232
Hin1II CATG 1 cut(s) 361
HinfI GANTC 1 cut(s) 251
Hpy188I TCNGA 2 cut(s) 319, 383
HpyCH4V TGCA 3 cut(s) 13, 331, 361
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 19, 28, 358
HpyF3I CTNAG 1 cut(s) 150
Hsp92II CATG 1 cut(s) 361
LmnI GCTCC 1 cut(s) 172
LpnPI CCDG 7 cut(s) 63, 170, 197, 281, 297, 338, 347
MaeI CTAG 2 cut(s) 5, 213
MaeIII GTNAC 1 cut(s) 124
MboII GAAGA 1 cut(s) 230
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 2 cut(s) 79, 283
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 5 cut(s) 112, 115, 271, 315, 338
Mox20I TGGCCA 1 cut(s) 356
MroXI GAANNNNTTC 1 cut(s) 81
MscI TGGCCA 1 cut(s) 356
Msp20I TGGCCA 1 cut(s) 356
MspR9I CCNGG 1 cut(s) 185
MvaI CCWGG 1 cut(s) 185
MwoI GCNNNNNNNGC 4 cut(s) 10, 19, 28, 358
NdeI CATATG 1 cut(s) 196
NlaIII CATG 1 cut(s) 361
NlaIV GGNNCC 1 cut(s) 366
PdmI GAANNNNTTC 1 cut(s) 81
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PpuMI RGGWCCY 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 364
Psp6I CCWGG 1 cut(s) 183
PspEI GGTNACC 1 cut(s) 124
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 366
PspPI GGNCC 1 cut(s) 364
PspPPI RGGWCCY 1 cut(s) 364
PstI CTGCAG 1 cut(s) 333
Sau96I GGNCC 1 cut(s) 364
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 185
SetI ASST 8 cut(s) 19, 24, 126, 131, 177, 214, 316, 354
SfcI CTRYAG 1 cut(s) 329
SinI GGWCC 1 cut(s) 364
Sse9I AATT 2 cut(s) 79, 283
SsiI CCGC 2 cut(s) 31, 295
SspMI CTAG 2 cut(s) 5, 213
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 168
TaqI TCGA 1 cut(s) 201
TasI AATT 2 cut(s) 79, 283
TscAI CASTG 1 cut(s) 338
TspRI CASTG 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 364
XapI RAATTY 2 cut(s) 79, 283
XmnI GAANNNNTTC 1 cut(s) 81
XspI CTAG 2 cut(s) 5, 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.