Rh1DG061600

aminoacylase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0022629
Physical Location & Seq
Forward (+)
10833730 .. 10841863
8134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG061600.1

Sequence Viewer

Length: 972 bp
ATGCAATGCCTGATTGAGCTGCTGAGGATGCTGAGGATGCTAAGACTGATGGCGGGGGTGGTAGATGGTGGGCGTGAGGTAGCGAGCGATGGGGACTGCACAAATGTGCCCGGTGAATGGGATGGCTATGGAGCAGACTTCACAACCGAAGGGAACCCGATTGAACTCCCAGAAAGGGTTGTGCCTGATGCTTACAGGGAGTGGGAGGTTAAGGTTTTCCACTGGCAGACTCAATGTCCCACTCTTGCTGACCCAGAAGACCAAGTTCTTACCTACAAGACGATAGATGTGAAGCCGGATGTGAAGCCGATGCTGCTACACGTGGAGGATAAGAGTTTGAATAAGTTGTTGGAAGTAGAGTATTGCTTGATCAATCCTCAAGATAAGGAGTCTCGTTTGAGGATAATTCAGGTGATTCTCATAGACAATATGAAGATGGTATTGCAGAGTCTTAGAGTTCTTTGTGAACAGTGGTATGGGCCATTCAGAAATGGGGAGCAATTAGGTGGATGTGCCATCCGTGATTCTGCTTTTGCTTCTACGCCCGCATTGGAAGCTTCAAAAGTTGTTGGTACTTGGCAGAGCTCCAGTGCTGTTGCCACTTTTAATGGTTCTCAAAATTTTGATGATACAAATCCTGCTAAAGAAAGCAATGAGTTTGTGGAAAATCTTCTCAAAGATATTGACACTTTGGGTATCAAGTATGAGGAGGTTACCTATACTTTAGATTACTTCTCTAAGTTGATGGATATGACCAAGGAGCTGATTATCCAGGGTAAAGCATATGTCGATGATACACCTAGAGAAGAGACGCAAAAACAACAGATAGATGGGATTGAGTCAAAGTGTAGAAACCAGAGCCAAGAGGAAAATTTGAAACTGCGGGGGGAGATGACAGCAGGTTCAAAGAGGGGTCTGCAGTGTTGTGTTAGAGGGAAGCTGGCCATGCAGGACCCTAATAAATCGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

323

Amino Acids

36.55

Weight (kDa)

4.76

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c PF00749 208 - 319 8.6e-24 tRNA synthetases class I (E and Q), catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022629)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0323471
rosa_multiflora Rmu_sc0019211.1_g000002
rosa_samantha Rh1CG057800 Rh1DG061600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 890
AciI CCGC 3 cut(s) 53, 546, 883
AcoI YGGCCR 1 cut(s) 942
AcsI RAATTY 2 cut(s) 619, 871
AcvI CACGTG 1 cut(s) 322
AfaI GTAC 1 cut(s) 574
AfiI CCNNNNNNNGG 2 cut(s) 117, 175
AflIII ACRYGT 1 cut(s) 319
AgsI TTSAA 5 cut(s) 164, 340, 561, 877, 906
AhdI GACNNNNNGTC 1 cut(s) 234
AjnI CCWGG 1 cut(s) 771
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AleI CACNNNNGTG 1 cut(s) 104
AluBI AGCT 5 cut(s) 19, 557, 585, 763, 940
AluI AGCT 5 cut(s) 19, 557, 585, 763, 940
Alw21I GWGCWC 1 cut(s) 587
Alw26I GTCTC 2 cut(s) 396, 803
AoxI GGCC 2 cut(s) 479, 942
ApeKI GCWGC 2 cut(s) 19, 313
ApoI RAATTY 2 cut(s) 619, 871
Asp700I GAANNNNTTC 1 cut(s) 669
AspS9I GGNCC 2 cut(s) 479, 952
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 2 cut(s) 125, 424
AvaII GGWCC 1 cut(s) 952
BaeGI GKGCMC 1 cut(s) 111
BalI TGGCCA 1 cut(s) 944
BanII GRGCYC 1 cut(s) 587
BbrPI CACGTG 1 cut(s) 322
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 587
BbvCI CCTCAGC 2 cut(s) 23, 32
BbvI GCAGC 2 cut(s) 6, 300
BccI CCATC 8 cut(s) 43, 59, 83, 116, 430, 524, 739, 824
BciT130I CCWGG 1 cut(s) 773
BclI TGATCA 1 cut(s) 369
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 2 cut(s) 396, 803
BfaI CTAG 1 cut(s) 801
BfmI CTRYAG 1 cut(s) 917
BfuAI ACCTGC 1 cut(s) 890
BisI GCNGC 2 cut(s) 20, 314
BlsI GCNGC 2 cut(s) 21, 315
Bme1390I CCNGG 2 cut(s) 111, 773
Bme18I GGWCC 1 cut(s) 952
BmeRI GACNNNNNGTC 1 cut(s) 234
BmgT120I GGNCC 2 cut(s) 479, 952
BmiI GGNNCC 2 cut(s) 155, 954
BmrFI CCNGG 2 cut(s) 111, 773
BmsI GCATC 4 cut(s) 18, 27, 178, 300
BpiI GAAGAC 1 cut(s) 264
BpmI CTGGAG 1 cut(s) 571
Bpu10I CCTNAGC 2 cut(s) 23, 32
BpuEI CTTGAG 1 cut(s) 363
BpuMI CCSGG 1 cut(s) 111
BsaAI YACGTR 1 cut(s) 322
BsaBI GATNNNNATC 1 cut(s) 633
BsaJI CCNNGG 2 cut(s) 756, 772
Bsc4I CCNNNNNNNGG 2 cut(s) 117, 175
Bse1I ACTGG 2 cut(s) 227, 588
Bse3DI GCAATG 2 cut(s) 11, 658
Bse8I GATNNNNATC 1 cut(s) 633
BseBI CCWGG 1 cut(s) 773
BseDI CCNNGG 2 cut(s) 756, 772
BseGI GGATG 6 cut(s) 33, 42, 127, 304, 515, 516
BseJI GATNNNNATC 1 cut(s) 633
BseLI CCNNNNNNNGG 2 cut(s) 117, 175
BseMI GCAATG 2 cut(s) 11, 658
BseMII CTCAG 2 cut(s) 14, 23
BseNI ACTGG 2 cut(s) 227, 588
BseRI GAGGAG 1 cut(s) 722
BseSI GKGCMC 1 cut(s) 111
BseXI GCAGC 2 cut(s) 6, 300
BsgI GTGCAG 1 cut(s) 82
BshFI GGCC 2 cut(s) 481, 944
BsiHKAI GWGCWC 1 cut(s) 587
BsiSI CCGG 2 cut(s) 111, 296
BslFI GGGAC 2 cut(s) 107, 222
BslI CCNNNNNNNGG 2 cut(s) 117, 175
BsmAI GTCTC 2 cut(s) 396, 803
BsmBI CGTCTC 1 cut(s) 803
BsmFI GGGAC 2 cut(s) 107, 222
BsnI GGCC 2 cut(s) 481, 944
Bsp1286I GDGCHC 2 cut(s) 111, 587
Bsp143I GATC 1 cut(s) 369
BspACI CCGC 3 cut(s) 53, 546, 883
BspANI GGCC 2 cut(s) 481, 944
BspCNI CTCAG 2 cut(s) 15, 24
BspLI GGNNCC 2 cut(s) 155, 954
BspMAI CTGCAG 1 cut(s) 921
BspMI ACCTGC 1 cut(s) 890
BsrDI GCAATG 2 cut(s) 11, 658
BsrI ACTGG 2 cut(s) 227, 588
BssECI CCNNGG 2 cut(s) 756, 772
BssMI GATC 1 cut(s) 369
BssT1I CCWWGG 1 cut(s) 756
Bst2UI CCWGG 1 cut(s) 773
Bst4CI ACNGT 1 cut(s) 471
Bst6I CTCTTC 1 cut(s) 801
BstBAI YACGTR 1 cut(s) 322
BstC8I GCNNGC 3 cut(s) 85, 546, 942
BstDEI CTNAG 5 cut(s) 23, 32, 41, 452, 738
BstEII GGTNACC 1 cut(s) 712
BstF5I GGATG 6 cut(s) 33, 42, 127, 304, 515, 516
BstKTI GATC 1 cut(s) 372
BstMAI GTCTC 2 cut(s) 396, 803
BstMBI GATC 1 cut(s) 369
BstMWI GCNNNNNNNGC 5 cut(s) 28, 37, 313, 554, 946
BstNI CCWGG 1 cut(s) 773
BstPI GGTNACC 1 cut(s) 712
BstSCI CCNGG 2 cut(s) 109, 771
BstSFI CTRYAG 1 cut(s) 917
BstSLI GKGCMC 1 cut(s) 111
BstV1I GCAGC 2 cut(s) 6, 300
BstV2I GAAGAC 1 cut(s) 264
BsuRI GGCC 2 cut(s) 481, 944
BtgZI GCGATG 1 cut(s) 102
BtsCI GGATG 6 cut(s) 33, 42, 127, 304, 515, 516
BtsI GCAGTG 1 cut(s) 926
BtsIMutI CAGTG 4 cut(s) 220, 476, 595, 926
BveI ACCTGC 1 cut(s) 890
Cac8I GCNNGC 3 cut(s) 85, 546, 942
Cfr13I GGNCC 2 cut(s) 479, 952
CseI GACGC 1 cut(s) 820
Csp6I GTAC 1 cut(s) 573
CviAII CATG 1 cut(s) 946
CviQI GTAC 1 cut(s) 573
DdeI CTNAG 5 cut(s) 23, 32, 41, 452, 738
DpnI GATC 1 cut(s) 371
DpnII GATC 1 cut(s) 369
DriI GACNNNNNGTC 1 cut(s) 234
EaeI YGGCCR 1 cut(s) 942
Eam1104I CTCTTC 1 cut(s) 801
Eam1105I GACNNNNNGTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 801
Ecl136II GAGCTC 1 cut(s) 585
Eco130I CCWWGG 1 cut(s) 756
Eco24I GRGCYC 1 cut(s) 587
Eco47I GGWCC 1 cut(s) 952
Eco53kI GAGCTC 1 cut(s) 585
Eco72I CACGTG 1 cut(s) 322
Eco91I GGTNACC 1 cut(s) 712
EcoICRI GAGCTC 1 cut(s) 585
EcoO109I RGGNCCY 1 cut(s) 952
EcoO65I GGTNACC 1 cut(s) 712
EcoRII CCWGG 1 cut(s) 771
EcoT14I CCWWGG 1 cut(s) 756
EcoT38I GRGCYC 1 cut(s) 587
ErhI CCWWGG 1 cut(s) 756
Esp3I CGTCTC 1 cut(s) 803
FaeI CATG 1 cut(s) 949
FaqI GGGAC 2 cut(s) 107, 222
FatI CATG 1 cut(s) 945
FauI CCCGC 3 cut(s) 46, 553, 876
FauNDI CATATG 1 cut(s) 784
FbaI TGATCA 1 cut(s) 369
Fnu4HI GCNGC 2 cut(s) 20, 314
FokI GGATG 6 cut(s) 40, 49, 134, 311, 503, 522
FriOI GRGCYC 1 cut(s) 587
Fsp4HI GCNGC 2 cut(s) 20, 314
FspBI CTAG 1 cut(s) 801
GluI GCNGC 2 cut(s) 20, 314
GsuI CTGGAG 1 cut(s) 571
HaeIII GGCC 2 cut(s) 481, 944
HapII CCGG 2 cut(s) 111, 296
HgaI GACGC 1 cut(s) 820
Hin1II CATG 1 cut(s) 949
HindIII AAGCTT 1 cut(s) 555
HinfI GANTC 6 cut(s) 229, 389, 415, 448, 524, 839
HpaII CCGG 2 cut(s) 111, 296
HphI GGTGA 2 cut(s) 125, 424
Hpy166II GTNNAC 1 cut(s) 467
Hpy188I TCNGA 2 cut(s) 488, 971
Hpy188III TCNNGA 1 cut(s) 380
Hpy8I GTNNAC 1 cut(s) 467
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 1 cut(s) 471
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 5 cut(s) 4, 99, 445, 919, 949
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 37, 313, 554, 946
HpyF3I CTNAG 5 cut(s) 23, 32, 41, 452, 738
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 1 cut(s) 949
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 1 cut(s) 369
LmnI GCTCC 4 cut(s) 131, 496, 590, 760
Lsp1109I GCAGC 2 cut(s) 6, 300
LweI GCATC 4 cut(s) 18, 27, 178, 300
MaeI CTAG 1 cut(s) 801
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 712
MalI GATC 1 cut(s) 371
MboI GATC 1 cut(s) 369
MboII GAAGA 4 cut(s) 269, 445, 662, 818
MhlI GDGCHC 2 cut(s) 111, 587
MlsI TGGCCA 1 cut(s) 944
MluCI AATT 4 cut(s) 405, 500, 619, 871
MluNI TGGCCA 1 cut(s) 944
MlyI GAGTC 4 cut(s) 223, 398, 457, 848
MmeI TCCRAC 1 cut(s) 330
Mox20I TGGCCA 1 cut(s) 944
MroXI GAANNNNTTC 1 cut(s) 669
MscI TGGCCA 1 cut(s) 944
MseI TTAA 2 cut(s) 210, 606
MslI CAYNNNNRTG 1 cut(s) 104
Msp20I TGGCCA 1 cut(s) 944
MspI CCGG 2 cut(s) 111, 296
MspR9I CCNGG 2 cut(s) 111, 773
MvaI CCWGG 1 cut(s) 773
MwoI GCNNNNNNNGC 5 cut(s) 28, 37, 313, 554, 946
NciI CCSGG 1 cut(s) 111
NdeI CATATG 1 cut(s) 784
NdeII GATC 1 cut(s) 369
NlaIII CATG 1 cut(s) 949
NlaIV GGNNCC 2 cut(s) 155, 954
OliI CACNNNNGTG 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 2 cut(s) 415, 524
PkrI GCNGC 2 cut(s) 21, 315
PleI GAGTC 4 cut(s) 223, 397, 456, 847
PmaCI CACGTG 1 cut(s) 322
PmlI CACGTG 1 cut(s) 322
PpsI GAGTC 4 cut(s) 223, 397, 456, 847
Ppu21I YACGTR 1 cut(s) 322
PpuMI RGGWCCY 1 cut(s) 952
Psp124BI GAGCTC 1 cut(s) 587
Psp5II RGGWCCY 1 cut(s) 952
Psp6I CCWGG 1 cut(s) 771
PspCI CACGTG 1 cut(s) 322
PspEI GGTNACC 1 cut(s) 712
PspGI CCWGG 1 cut(s) 771
PspN4I GGNNCC 2 cut(s) 155, 954
PspPI GGNCC 2 cut(s) 479, 952
PspPPI RGGWCCY 1 cut(s) 952
PstI CTGCAG 1 cut(s) 921
RsaI GTAC 1 cut(s) 574
RsaNI GTAC 1 cut(s) 573
RseI CAYNNNNRTG 1 cut(s) 104
SacI GAGCTC 1 cut(s) 587
SaqAI TTAA 2 cut(s) 210, 606
SatI GCNGC 2 cut(s) 20, 314
Sau3AI GATC 1 cut(s) 369
Sau96I GGNCC 2 cut(s) 479, 952
SchI GAGTC 4 cut(s) 223, 398, 457, 848
ScrFI CCNGG 2 cut(s) 111, 773
SduI GDGCHC 2 cut(s) 111, 587
SfaNI GCATC 4 cut(s) 18, 27, 178, 300
SfcI CTRYAG 1 cut(s) 917
SinI GGWCC 1 cut(s) 952
SmiMI CAYNNNNRTG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
Sse9I AATT 4 cut(s) 405, 500, 619, 871
SsiI CCGC 3 cut(s) 53, 546, 883
SspMI CTAG 1 cut(s) 801
SstI GAGCTC 1 cut(s) 587
StyD4I CCNGG 2 cut(s) 109, 771
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 1 cut(s) 471
TaiI ACGT 1 cut(s) 324
TaqI TCGA 1 cut(s) 789
TasI AATT 4 cut(s) 405, 500, 619, 871
TfiI GAWTC 2 cut(s) 415, 524
Tru1I TTAA 2 cut(s) 210, 606
Tru9I TTAA 2 cut(s) 210, 606
TscAI CASTG 4 cut(s) 227, 476, 595, 926
TseI GCWGC 2 cut(s) 19, 313
TspDTI ATGAA 1 cut(s) 446
TspGWI ACGGA 1 cut(s) 509
TspRI CASTG 4 cut(s) 227, 476, 595, 926
VpaK11BI GGWCC 1 cut(s) 952
XapI RAATTY 2 cut(s) 619, 871
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.