RchiOBHm_Chr2g0084811

LETM1 and EF-hand domain-containing protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007779
Physical Location & Seq
Forward (+)
458451 .. 465332
6882 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46038

Sequence Viewer

Length: 2163 bp
ATGGCTTCGAGAGCGATTTTCAGACGGAGCAGATTGTTGAGGGACTATGTGAGCGCCTCCTCCCGCACAACCCGGTGGCTGGGGCATTGCCACGCTGGCTTGAATTCCTCTTTCAGCACCGGCGTCAATGATGACGATAGATTTTCGATTGCCAAAGAGGAATTCCGGCGATTCCCCCAGCCACCTCCGATGGGTGTCAGATGGATCGCTCAGTCCGTTCGTACTGTCACCACCGCCACCGCGCCGGCCAAACCGCCGCCCCCGGACGATGAGGATAACGAAGGCCGGGTCTGCGACAAAAGAAAGCAAGCTTCTCCGGAGGATTGCGATCAAGCTGTGCAAGGCCTCAGCTCCACTGCCAAAGCCGCCAAGGCCAAGAAGCTGCAGGGGTCTCAGAAGGTTTCCAAAATGTTGAAGAAAGTGTGGGCCACTTTTTTGGGGATCGGTCCCGCTTTGAGAGCTGTGGCTTCCATGAGCAGGGAGGACTGGGCCAATAAGCTTGCTCACTGGAAAAAGGAGATTGTGTCAACATTGCAGCATTACTGGTTGGGTTTTAAGCTACTCGGCACTGATGTGAGGATCTGTTCGAGATTGCTGCTCAAGCTTGCTGGTGGCAAGACTCTCTCCAGAAGGGAGAGGCAGCAGCTGACGCGTACAACAGCTGATATCTTTAGGCTAGTTCCATTTGCAGTGTTTATCATTGTTCCATTCATGGAATTCTTGCTGCCAGTATTCCTCAAGTTGTTTCCCAACATGTTGCCTTCAACCTTTCAAGACAAATTGAAAGAAGAGGAAGCAATGAAAAGGAAGCTAAAGGCAAGAATAGAGTATGCTAAGTTCCTTCAAGACACAGCCAAAGAAATGGCAAAGGAAGTTCAAAACTCAAGGAGCGGAGAAATCAAACAAACAGCAGAAGATCTTGATGGGTTTTTAAGCAGGCTGAGGACGGGTGCAGGGGTCTCTAATGAGGAGATTTTGGGGTTTGCCAAGTTGTTTAATGATGAACTTACCCTTGATAACATCAGCAGGCCCCGTTTAGTCAATATGTGCAAATATATGGGTATAAGCCCTTATGGTACAGATGCATATTTGAGATATATGCTCCGGCAAAGACTCAGGCGGATTAAAGCTGATGATAAGTTAATTCAAGCGGAGGGTGTTGAGTCACTTTCAGAAGACGAGCTTCGTGAGGATTGCAGGGAGCGGGGTATGCTTGGATTGCGTTCTGTGGAAGAAATGCGTCAACAGCTTCGTGATTGGTTGGATTTGTCTCTTAATCACTCCGTGCCATCCTCACTTTTGATCCTTTCCAGGGCCTTCTATGTGTCTGGAAAATTGAAGCCAGAGGAAGCTGTTCGAGCTACACTGACTTCTATGCCAGATGAAGTGGTGGATACTATTAATGTTACATCATTGCCATCTGAAGATTCTGTTTCTGAAAGGAGGAGGAAGTTGGAGTACTTAGAGATGCAGGAAGAAATGATCAAGGAGGAAGAAGAGCGGGAGGAAGAAGAGCAACGCAGAATCAAGGAATCTAAAGCTAGTCAAGAAGATGTGGCATTGAAAGAGATGACTATTCCCACAGCAAGAGAAGCACAAGAGCAAGCCAAGGAAAGGACACTAGAAAAGCAAGAGCAGCTTTGCGAACTAAGTCGTGCTTTGGCTGTTTTAGCTTCTGCATCTTCAGTGAGTACAGAGCGTGAAGAATTCCTGAGGCTCGTAAGCAAGGAGATTGAACTTTACAATAGCATGGTGGAAAAGGATGGTACAGATGGTGAAAAGGATGCCTTCAAGGCTTATAGAGCTGCTCGAGATGAAACTGATACTGCTGGTGTTGAAGACGAAGCTGATCATGTTTCATCAGCACTCATAGATAAGGTTGATTCGATGCTTCAAAATCTGGAAAAAGAAATTGATGATGTGGATGCGCAAATTGGTGATCGCTACCAACTACTTGACAGGGATTTTGATGGGAAAGTAACTGCTGAGGAGGTTGCAGCTGCTGCAAACTATCTGAAGAATACTTTAGGCAAGGAAGGTGTCCAAGAACTCATAGGCAACCTCTCGAAAGATAGAGATGGTAAAATTCTTGTGGAAGACATTGTGAAATTGGGAAGTCGGACTGAAGATGCAAACAACCCAGATGAAGATGGGCGAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 ko04139 ko04212 ko05152 map03018 map04139 map04212 map05152
Pfam Domains
Protein Families
LETM1

Protein Analysis

720

Amino Acids

81.63

Weight (kDa)

5.57

Isoelectric Point (pI)

55.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LETM1_RBD PF07766 208 - 441 3.2e-86 LETM1-like, RBD
EF-hand_7 PF13499 643 - 703 2.9e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1929
AccBSI CCGCTC 3 cut(s) 891, 1204, 1501
AccII CGCG 2 cut(s) 242, 652
AccIII TCCGGA 1 cut(s) 316
AclWI GGATC 4 cut(s) 212, 449, 587, 1297
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 5 cut(s) 103, 161, 716, 1706, 2085
AcuI CTGAAG 4 cut(s) 1443, 1668, 2036, 2145
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 1285
AfaI GTAC 6 cut(s) 223, 655, 1078, 1460, 1693, 1768
AfiI CCNNNNNNNGG 5 cut(s) 79, 191, 477, 1312, 1614
AflIII ACRYGT 2 cut(s) 650, 753
AjnI CCWGG 1 cut(s) 1310
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AleI CACNNNNGTG 1 cut(s) 572
Alw26I GTCTC 3 cut(s) 396, 964, 1275
AlwI GGATC 4 cut(s) 212, 449, 587, 1297
AlwNI CAGNNNCTG 2 cut(s) 646, 2003
Ama87I CYCGRG 1 cut(s) 1809
Aor13HI TCCGGA 1 cut(s) 316
AoxI GGCC 8 cut(s) 246, 283, 343, 372, 426, 489, 1028, 1314
ApoI RAATTY 5 cut(s) 103, 161, 716, 1706, 2085
ArsI GACNNNNNNTTYG 4 cut(s) 273, 305, 1949, 1981
AseI ATTAAT 1 cut(s) 1401
Asp700I GAANNNNTTC 1 cut(s) 1353
AspLEI GCGC 3 cut(s) 56, 244, 1930
AspS9I GGNCC 5 cut(s) 426, 446, 489, 1029, 1314
AsuC2I CCSGG 3 cut(s) 73, 263, 287
AsuHPI GGTGA 3 cut(s) 220, 1787, 1949
AvaI CYCGRG 1 cut(s) 1809
AvaII GGWCC 1 cut(s) 446
AxyI CCTNAGG 1 cut(s) 1712
BarI GAAGNNNNNNTAC 2 cut(s) 1442, 1474
BbsI GAAGAC 3 cut(s) 1182, 1845, 2103
BbvCI CCTCAGC 3 cut(s) 347, 941, 1986
BcgI CGANNNNNNTGC 2 cut(s) 577, 611
BciT130I CCWGG 1 cut(s) 1312
BciVI GTATCC 1 cut(s) 1387
BclI TGATCA 2 cut(s) 1482, 1849
BcnI CCSGG 3 cut(s) 73, 263, 287
BcoDI GTCTC 3 cut(s) 396, 964, 1275
BfaI CTAG 3 cut(s) 677, 1542, 1622
BfmI CTRYAG 1 cut(s) 383
BfoI RGCGCY 1 cut(s) 57
BfuI GTATCC 1 cut(s) 1387
BglI GCCNNNNNGGC 3 cut(s) 96, 371, 1793
BglII AGATCT 1 cut(s) 916
BmcAI AGTACT 1 cut(s) 1460
Bme1390I CCNGG 4 cut(s) 73, 263, 287, 1312
Bme18I GGWCC 1 cut(s) 446
BmeT110I CYCGRG 1 cut(s) 1809
BmgT120I GGNCC 5 cut(s) 426, 446, 489, 1029, 1314
BmiI GGNNCC 2 cut(s) 448, 1031
BmrFI CCNGG 4 cut(s) 73, 263, 287, 1312
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 7 cut(s) 1072, 1458, 1688, 1774, 1878, 1915, 2119
BmuI ACTGGG 1 cut(s) 496
BpiI GAAGAC 3 cut(s) 1182, 1845, 2103
BpmI CTGGAG 1 cut(s) 610
Bpu10I CCTNAGC 3 cut(s) 347, 941, 1986
BpuEI CTTGAG 3 cut(s) 584, 722, 868
BpuMI CCSGG 3 cut(s) 73, 263, 287
BsaBI GATNNNNATC 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 2 cut(s) 396, 964
BsaJI CCNNGG 4 cut(s) 261, 369, 1311, 1608
BsaWI WCCGGW 1 cut(s) 316
BsaXI ACNNNNNCTCC 2 cut(s) 509, 539
Bsc4I CCNNNNNNNGG 5 cut(s) 79, 191, 477, 1312, 1614
Bse118I RCCGGY 2 cut(s) 119, 244
Bse1I ACTGG 4 cut(s) 491, 512, 548, 728
Bse21I CCTNAGG 1 cut(s) 1712
Bse3DI GCAATG 4 cut(s) 85, 530, 804, 1412
Bse8I GATNNNNATC 1 cut(s) 327
BseAI TCCGGA 1 cut(s) 316
BseBI CCWGG 1 cut(s) 1312
BseDI CCNNGG 4 cut(s) 261, 369, 1311, 1608
BseGI GGATG 4 cut(s) 1289, 1768, 1789, 1930
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 5 cut(s) 79, 191, 477, 1312, 1614
BseMI GCAATG 4 cut(s) 85, 530, 804, 1412
BseMII CTCAG 7 cut(s) 224, 361, 407, 932, 1129, 1703, 1977
BseNI ACTGG 4 cut(s) 491, 512, 548, 728
BseRI GAGGAG 4 cut(s) 49, 983, 1459, 2003
BseYI CCCAGC 2 cut(s) 79, 177
BsgI GTGCAG 1 cut(s) 972
Bsh1236I CGCG 2 cut(s) 242, 652
BshFI GGCC 8 cut(s) 248, 285, 345, 374, 428, 491, 1030, 1316
BsiHKCI CYCGRG 1 cut(s) 1809
BsiSI CCGG 8 cut(s) 73, 120, 166, 245, 263, 286, 317, 1105
BslFI GGGAC 2 cut(s) 56, 432
BslI CCNNNNNNNGG 5 cut(s) 79, 191, 477, 1312, 1614
BsmAI GTCTC 3 cut(s) 396, 964, 1275
BsmFI GGGAC 2 cut(s) 56, 432
BsnI GGCC 8 cut(s) 248, 285, 345, 374, 428, 491, 1030, 1316
Bso31I GGTCTC 2 cut(s) 396, 964
BsoBI CYCGRG 1 cut(s) 1809
Bsp13I TCCGGA 1 cut(s) 316
Bsp143I GATC 9 cut(s) 204, 328, 441, 579, 916, 1302, 1482, 1849, 1939
BspANI GGCC 8 cut(s) 248, 285, 345, 374, 428, 491, 1030, 1316
BspCNI CTCAG 7 cut(s) 223, 360, 406, 933, 1128, 1704, 1978
BspEI TCCGGA 1 cut(s) 316
BspFNI CGCG 2 cut(s) 242, 652
BspLI GGNNCC 2 cut(s) 448, 1031
BspMAI CTGCAG 1 cut(s) 387
BspPI GGATC 4 cut(s) 212, 449, 587, 1297
BspQI GCTCTTC 2 cut(s) 1491, 1506
BspTNI GGTCTC 2 cut(s) 396, 964
BsrBI CCGCTC 3 cut(s) 891, 1204, 1501
BsrDI GCAATG 4 cut(s) 85, 530, 804, 1412
BsrFI RCCGGY 2 cut(s) 119, 244
BsrI ACTGG 4 cut(s) 491, 512, 548, 728
BssAI RCCGGY 2 cut(s) 119, 244
BssECI CCNNGG 4 cut(s) 261, 369, 1311, 1608
BssMI GATC 9 cut(s) 204, 328, 441, 579, 916, 1302, 1482, 1849, 1939
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 2 cut(s) 369, 1608
Bst2UI CCWGG 1 cut(s) 1312
Bst4CI ACNGT 1 cut(s) 226
Bst6I CTCTTC 3 cut(s) 783, 1491, 1506
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 1 cut(s) 2003
BstC8I GCNNGC 8 cut(s) 97, 246, 309, 501, 606, 938, 1028, 1605
BstF5I GGATG 4 cut(s) 1289, 1768, 1789, 1930
BstFNI CGCG 2 cut(s) 242, 652
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 3 cut(s) 56, 244, 1930
BstKTI GATC 9 cut(s) 207, 331, 444, 582, 919, 1305, 1485, 1852, 1942
BstMAI GTCTC 3 cut(s) 396, 964, 1275
BstMBI GATC 9 cut(s) 204, 328, 441, 579, 916, 1302, 1482, 1849, 1939
BstNI CCWGG 1 cut(s) 1312
BstNSI RCATGY 1 cut(s) 757
BstSCI CCNGG 4 cut(s) 71, 261, 285, 1310
BstSFI CTRYAG 1 cut(s) 383
BstUI CGCG 2 cut(s) 242, 652
BstV2I GAAGAC 3 cut(s) 1182, 1845, 2103
BstX2I RGATCY 2 cut(s) 579, 916
BstXI CCANNNNNNTGG 2 cut(s) 436, 862
BstYI RGATCY 2 cut(s) 579, 916
Bsu36I CCTNAGG 1 cut(s) 1712
BsuI GTATCC 1 cut(s) 1387
BsuRI GGCC 8 cut(s) 248, 285, 345, 374, 428, 491, 1030, 1316
BtsCI GGATG 4 cut(s) 1289, 1768, 1789, 1930
BtsI GCAGTG 2 cut(s) 354, 696
BtsIMutI CAGTG 6 cut(s) 354, 505, 567, 696, 1364, 1692
Cac8I GCNNGC 8 cut(s) 97, 246, 309, 501, 606, 938, 1028, 1605
CaiI CAGNNNCTG 2 cut(s) 646, 2003
CfoI GCGC 3 cut(s) 56, 244, 1930
Cfr10I RCCGGY 2 cut(s) 119, 244
Cfr13I GGNCC 5 cut(s) 426, 446, 489, 1029, 1314
CseI GACGC 3 cut(s) 112, 658, 1229
Csp6I GTAC 6 cut(s) 222, 654, 1077, 1459, 1692, 1767
CviAII CATG 5 cut(s) 472, 712, 754, 1750, 1853
CviQI GTAC 6 cut(s) 222, 654, 1077, 1459, 1692, 1767
DpnI GATC 9 cut(s) 206, 330, 443, 581, 918, 1304, 1484, 1851, 1941
DpnII GATC 9 cut(s) 204, 328, 441, 579, 916, 1302, 1482, 1849, 1939
DraIII CACNNNGTG 1 cut(s) 1285
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 3 cut(s) 783, 1491, 1506
EarI CTCTTC 3 cut(s) 783, 1491, 1506
EciI GGCGGA 1 cut(s) 1135
Eco130I CCWWGG 2 cut(s) 369, 1608
Eco147I AGGCCT 1 cut(s) 345
Eco31I GGTCTC 2 cut(s) 396, 964
Eco32I GATATC 1 cut(s) 667
Eco47I GGWCC 1 cut(s) 446
Eco57I CTGAAG 4 cut(s) 1443, 1668, 2036, 2145
Eco81I CCTNAGG 1 cut(s) 1712
Eco88I CYCGRG 1 cut(s) 1809
EcoO109I RGGNCCY 2 cut(s) 1029, 1314
EcoRI GAATTC 4 cut(s) 103, 161, 716, 1706
EcoRII CCWGG 1 cut(s) 1310
EcoRV GATATC 1 cut(s) 667
EcoT14I CCWWGG 2 cut(s) 369, 1608
EcoT22I ATGCAT 1 cut(s) 1087
ErhI CCWWGG 2 cut(s) 369, 1608
FaeI CATG 5 cut(s) 475, 715, 757, 1753, 1856
FalI AAGNNNNNCTT 8 cut(s) 1167, 1199, 1623, 1655, 1642, 1674, 1772, 1804
FaqI GGGAC 2 cut(s) 56, 432
FatI CATG 5 cut(s) 471, 711, 753, 1749, 1852
FauI CCCGC 4 cut(s) 71, 457, 1197, 1494
FbaI TGATCA 2 cut(s) 1482, 1849
FokI GGATG 4 cut(s) 1276, 1775, 1796, 1937
FspBI CTAG 3 cut(s) 677, 1542, 1622
FspI TGCGCA 1 cut(s) 1929
GlaI GCGC 3 cut(s) 55, 243, 1929
GsaI CCCAGC 2 cut(s) 83, 181
GsuI CTGGAG 1 cut(s) 610
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 8 cut(s) 248, 285, 345, 374, 428, 491, 1030, 1316
HapII CCGG 8 cut(s) 73, 120, 166, 245, 263, 286, 317, 1105
HgaI GACGC 3 cut(s) 112, 658, 1229
HhaI GCGC 3 cut(s) 56, 244, 1930
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 5 cut(s) 475, 715, 757, 1753, 1856
Hin6I GCGC 3 cut(s) 54, 242, 1928
HinP1I GCGC 3 cut(s) 54, 242, 1928
HincII GTYRAC 2 cut(s) 528, 1244
HindII GTYRAC 2 cut(s) 528, 1244
HindIII AAGCTT 3 cut(s) 309, 497, 602
HinfI GANTC 8 cut(s) 171, 619, 1113, 1163, 1427, 1524, 1532, 1883
HpaII CCGG 8 cut(s) 73, 120, 166, 245, 263, 286, 317, 1105
HphI GGTGA 3 cut(s) 220, 1787, 1949
Hpy166II GTNNAC 2 cut(s) 528, 1244
Hpy188I TCNGA 9 cut(s) 23, 189, 200, 396, 1174, 1423, 1438, 2016, 2121
Hpy8I GTNNAC 2 cut(s) 528, 1244
HpyAV CCTTC 8 cut(s) 275, 391, 624, 771, 851, 1327, 1798, 2030
HpyCH4III ACNGT 1 cut(s) 226
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 5 cut(s) 475, 715, 757, 1753, 1856
HspAI GCGC 3 cut(s) 54, 242, 1928
Kpn2I TCCGGA 1 cut(s) 316
KroI GCCGGC 1 cut(s) 244
KroNI GCCGGC 1 cut(s) 246
Ksp22I TGATCA 2 cut(s) 1482, 1849
Kzo9I GATC 9 cut(s) 204, 328, 441, 579, 916, 1302, 1482, 1849, 1939
LguI GCTCTTC 2 cut(s) 1491, 1506
LmnI GCTCC 5 cut(s) 27, 356, 888, 1107, 1201
LweI GCATC 7 cut(s) 1072, 1458, 1688, 1774, 1878, 1915, 2119
MaeI CTAG 3 cut(s) 677, 1542, 1622
MaeIII GTNAC 4 cut(s) 226, 1164, 1405, 1978
MalI GATC 9 cut(s) 206, 330, 443, 581, 918, 1304, 1484, 1851, 1941
MbiI CCGCTC 3 cut(s) 891, 1204, 1501
MboI GATC 9 cut(s) 204, 328, 441, 579, 916, 1302, 1482, 1849, 1939
MflI RGATCY 2 cut(s) 579, 916
MluI ACGCGT 1 cut(s) 650
MlyI GAGTC 3 cut(s) 613, 1107, 1172
MmeI TCCRAC 3 cut(s) 1242, 1434, 2099
Mph1103I ATGCAT 1 cut(s) 1087
MroI TCCGGA 1 cut(s) 316
MroNI GCCGGC 1 cut(s) 244
MroXI GAANNNNTTC 1 cut(s) 1353
MseI TTAA 7 cut(s) 555, 932, 996, 1125, 1142, 1275, 1401
MslI CAYNNNNRTG 1 cut(s) 572
MspA1I CMGCKG 3 cut(s) 646, 662, 2000
MspI CCGG 8 cut(s) 73, 120, 166, 245, 263, 286, 317, 1105
MspR9I CCNGG 4 cut(s) 73, 263, 287, 1312
MvaI CCWGG 1 cut(s) 1312
MvnI CGCG 2 cut(s) 242, 652
NaeI GCCGGC 1 cut(s) 246
NciI CCSGG 3 cut(s) 73, 263, 287
NdeII GATC 9 cut(s) 204, 328, 441, 579, 916, 1302, 1482, 1849, 1939
NgoMIV GCCGGC 1 cut(s) 244
NlaIII CATG 5 cut(s) 475, 715, 757, 1753, 1856
NlaIV GGNNCC 2 cut(s) 448, 1031
NmeAIII GCCGAG 1 cut(s) 543
NmuCI GTSAC 2 cut(s) 226, 1164
NsbI TGCGCA 1 cut(s) 1929
NsiI ATGCAT 1 cut(s) 1087
NspI RCATGY 1 cut(s) 757
OliI CACNNNNGTG 1 cut(s) 572
PaeR7I CTCGAG 1 cut(s) 1809
PceI AGGCCT 1 cut(s) 345
PciI ACATGT 1 cut(s) 753
PciSI GCTCTTC 2 cut(s) 1491, 1506
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PdiI GCCGGC 1 cut(s) 246
PdmI GAANNNNTTC 1 cut(s) 1353
PfeI GAWTC 5 cut(s) 171, 1427, 1524, 1532, 1883
PleI GAGTC 3 cut(s) 613, 1107, 1171
PpsI GAGTC 3 cut(s) 613, 1107, 1171
PscI ACATGT 1 cut(s) 753
PshBI ATTAAT 1 cut(s) 1401
Psp6I CCWGG 1 cut(s) 1310
PspFI CCCAGC 2 cut(s) 79, 177
PspGI CCWGG 1 cut(s) 1310
PspN4I GGNNCC 2 cut(s) 448, 1031
PspPI GGNCC 5 cut(s) 426, 446, 489, 1029, 1314
PsrI GAACNNNNNNTAC 2 cut(s) 821, 853
PstI CTGCAG 1 cut(s) 387
PstNI CAGNNNCTG 2 cut(s) 646, 2003
PsuI RGATCY 2 cut(s) 579, 916
PvuII CAGCTG 3 cut(s) 646, 662, 2000
RsaI GTAC 6 cut(s) 223, 655, 1078, 1460, 1693, 1768
RsaNI GTAC 6 cut(s) 222, 654, 1077, 1459, 1692, 1767
RseI CAYNNNNRTG 1 cut(s) 572
SapI GCTCTTC 2 cut(s) 1491, 1506
SaqAI TTAA 7 cut(s) 555, 932, 996, 1125, 1142, 1275, 1401
Sau3AI GATC 9 cut(s) 204, 328, 441, 579, 916, 1302, 1482, 1849, 1939
Sau96I GGNCC 5 cut(s) 426, 446, 489, 1029, 1314
ScaI AGTACT 1 cut(s) 1460
SchI GAGTC 3 cut(s) 613, 1107, 1172
ScrFI CCNGG 4 cut(s) 73, 263, 287, 1312
SfaNI GCATC 7 cut(s) 1072, 1458, 1688, 1774, 1878, 1915, 2119
SfcI CTRYAG 1 cut(s) 383
Sfr274I CTCGAG 1 cut(s) 1809
SgrAI CRCCGGYG 1 cut(s) 119
SinI GGWCC 1 cut(s) 446
SlaI CTCGAG 1 cut(s) 1809
SmiMI CAYNNNNRTG 1 cut(s) 572
SmlI CTYRAG 4 cut(s) 599, 737, 883, 1809
SmoI CTYRAG 4 cut(s) 599, 737, 883, 1809
SseBI AGGCCT 1 cut(s) 345
SspMI CTAG 3 cut(s) 677, 1542, 1622
StuI AGGCCT 1 cut(s) 345
StyD4I CCNGG 4 cut(s) 71, 261, 285, 1310
StyI CCWWGG 2 cut(s) 369, 1608
TaaI ACNGT 1 cut(s) 226
TaqI TCGA 7 cut(s) 8, 146, 587, 1357, 1810, 1886, 2066
TaqII GACCGA 1 cut(s) 434
TatI WGTACW 2 cut(s) 1458, 1691
TauI GCSGC 2 cut(s) 259, 368
TfiI GAWTC 5 cut(s) 171, 1427, 1524, 1532, 1883
Tru1I TTAA 7 cut(s) 555, 932, 996, 1125, 1142, 1275, 1401
Tru9I TTAA 7 cut(s) 555, 932, 996, 1125, 1142, 1275, 1401
TscAI CASTG 6 cut(s) 361, 512, 574, 696, 1371, 1692
TseFI GTSAC 2 cut(s) 226, 1164
Tsp45I GTSAC 2 cut(s) 226, 1164
TspDTI ATGAA 7 cut(s) 700, 815, 1017, 1398, 1830, 1848, 2160
TspGWI ACGGA 3 cut(s) 40, 205, 1273
TspRI CASTG 6 cut(s) 361, 512, 574, 696, 1371, 1692
VpaK11BI GGWCC 1 cut(s) 446
VspI ATTAAT 1 cut(s) 1401
XapI RAATTY 5 cut(s) 103, 161, 716, 1706, 2085
XceI RCATGY 1 cut(s) 757
XhoI CTCGAG 1 cut(s) 1809
XmnI GAANNNNTTC 1 cut(s) 1353
XspI CTAG 3 cut(s) 677, 1542, 1622
ZrmI AGTACT 1 cut(s) 1460
Zsp2I ATGCAT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.