Rorug01G0456500

LETM1 and EF-hand domain-containing protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007779
Physical Location & Seq
Reverse (-)
55071034 .. 55071957
924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0456500.1

Sequence Viewer

Length: 924 bp
ATGGCCTTACAAATGTCTATGGGTGAGCTTTCAACTTCAGTGGGTGATCCTGAATGCAGTAATTCTCATCAGTCAGAACTGTCTGTACAACAAGGGGAATTGATCAGGACTTTTTTGAAGAACAATGCTAGTCAATTGACTTACTATGGCCTTTTCTGCTTACAAGATGGCCTTAAAGAGCAGGAGCTCTGTGTTTTCTTTCGAAACAACCACTTCAGTACCATGTTTAAGATCAATGGAGAACTTTATCTTTTGGCAACTGATCAGGGTTACCTGAATCAGCATGATTTGGTGTGGGAAAAGCTTAATGAGGTGAATGGAGATACATTGTTCATGACTGGCAATTTCAAGGAATTTAAAGTTGAAAACCAATCAAATGAAAATTGGACCACTGCCGACTACCTTGCCAGCATTGATAGTGCAGCTTCAGCAGGCTTCGATATAAATTCCGATCTGCAATTGGCAATTGCTCTGCAACAACAGGACTACTGTAGCGGAAATTCAACGAAGCTTGACGATGGAGAAAAGAGTGGTCTAGTTCTGACTCCTCCTCAAGTTCTTGAAGGGTCCAAGAAAAAAACTGTTCTTGTCAATTTCATGGAGTACTGTAGGGCTATGGTTAGGGAGCCGGATCATGTAATGAGTTTCATGCTTGCGGAATTGGACACGAGTGGATCGCTTGATGAACAGCTGAGGTTAGTTGTGAAGGGAAGATTTTTGCCTAAGAAGTTCGGAGCAATCTTGCGGAAATATGTCAATCAATATGTGATTTGCTTCGCATGCAAACGTACAGACACTCGACTTTCGAAAGAAAATCGGCCCAAGTACCATCTTATATGCGAGTGGTGTTGTGCTTCGCGATCAGTTGCTCCTATTAAAGCTGGTTTTGTTGCCAAAGTTGGAAAAAGGAACAGTGCGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 ko04139 ko04212 ko05152 map03018 map04139 map04212 map05152
Pfam Domains
Protein Families
LETM1

Protein Analysis

307

Amino Acids

34.54

Weight (kDa)

6.01

Isoelectric Point (pI)

28.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MINDY_DUB PF04424 21 - 116 5.2e-38 MINDY deubiquitinase
eIF-5_eIF-2B PF01873 180 - 285 2.4e-29 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 859
AciI CCGC 4 cut(s) 495, 656, 745, 917
AclWI GGATC 3 cut(s) 41, 639, 682
AcsI RAATTY 3 cut(s) 353, 445, 499
AcuI CTGAAG 3 cut(s) 21, 199, 411
AfaI GTAC 5 cut(s) 87, 220, 605, 790, 827
AgsI TTSAA 6 cut(s) 33, 118, 349, 365, 504, 563
AluBI AGCT 7 cut(s) 28, 187, 304, 425, 511, 691, 881
AluI AGCT 7 cut(s) 28, 187, 304, 425, 511, 691, 881
Alw21I GWGCWC 1 cut(s) 189
AlwI GGATC 3 cut(s) 41, 639, 682
AoxI GGCC 4 cut(s) 3, 148, 169, 818
ApeKI GCWGC 1 cut(s) 422
ApoI RAATTY 3 cut(s) 353, 445, 499
AspS9I GGNCC 3 cut(s) 387, 567, 819
AsuHPI GGTGA 3 cut(s) 35, 56, 325
AsuII TTCGAA 2 cut(s) 202, 806
AvaII GGWCC 2 cut(s) 387, 567
BaeI ACNNNNGTAYC 2 cut(s) 202, 235
BanII GRGCYC 1 cut(s) 189
BauI CACGAG 1 cut(s) 667
Bbv12I GWGCWC 1 cut(s) 189
BbvCI CCTCAGC 1 cut(s) 692
BbvI GCAGC 1 cut(s) 434
BccI CCATC 3 cut(s) 161, 512, 837
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BclI TGATCA 2 cut(s) 102, 262
BfaI CTAG 2 cut(s) 129, 536
BfmI CTRYAG 2 cut(s) 490, 607
BisI GCNGC 2 cut(s) 423, 918
BlsI GCNGC 2 cut(s) 424, 919
BmcAI AGTACT 1 cut(s) 605
Bme18I GGWCC 2 cut(s) 387, 567
BmgT120I GGNCC 3 cut(s) 387, 567, 819
BmiI GGNNCC 2 cut(s) 568, 627
Bpu10I CCTNAGC 1 cut(s) 692
Bpu14I TTCGAA 2 cut(s) 202, 806
BpuEI CTTGAG 1 cut(s) 537
Bse1I ACTGG 1 cut(s) 343
BseMII CTCAG 1 cut(s) 683
BseNI ACTGG 1 cut(s) 343
BseRI GAGGAG 2 cut(s) 537, 540
BseXI GCAGC 1 cut(s) 434
BsgI GTGCAG 1 cut(s) 441
Bsh1236I CGCG 1 cut(s) 859
BshFI GGCC 4 cut(s) 5, 150, 171, 820
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 1 cut(s) 629
BsmI GAATGC 1 cut(s) 59
BsnI GGCC 4 cut(s) 5, 150, 171, 820
Bsp119I TTCGAA 2 cut(s) 202, 806
Bsp1286I GDGCHC 1 cut(s) 189
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 8 cut(s) 46, 102, 231, 262, 451, 631, 674, 860
Bsp68I TCGCGA 1 cut(s) 859
BspACI CCGC 4 cut(s) 495, 656, 745, 917
BspANI GGCC 4 cut(s) 5, 150, 171, 820
BspCNI CTCAG 1 cut(s) 684
BspFNI CGCG 1 cut(s) 859
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 2 cut(s) 568, 627
BspPI GGATC 3 cut(s) 41, 639, 682
BspT104I TTCGAA 2 cut(s) 202, 806
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 1 cut(s) 343
BssMI GATC 8 cut(s) 46, 102, 231, 262, 451, 631, 674, 860
BssSI CACGAG 1 cut(s) 667
Bst2BI CACGAG 1 cut(s) 667
Bst4CI ACNGT 5 cut(s) 81, 491, 583, 608, 914
BstAUI TGTACA 1 cut(s) 85
BstBI TTCGAA 2 cut(s) 202, 806
BstC8I GCNNGC 4 cut(s) 409, 433, 654, 781
BstDEI CTNAG 2 cut(s) 692, 723
BstEII GGTNACC 1 cut(s) 269
BstFNI CGCG 1 cut(s) 859
BstKTI GATC 8 cut(s) 49, 105, 234, 265, 454, 634, 677, 863
BstMBI GATC 8 cut(s) 46, 102, 231, 262, 451, 631, 674, 860
BstMWI GCNNNNNNNGC 3 cut(s) 156, 428, 780
BstNSI RCATGY 1 cut(s) 783
BstPI GGTNACC 1 cut(s) 269
BstSFI CTRYAG 2 cut(s) 490, 607
BstUI CGCG 1 cut(s) 859
BstV1I GCAGC 1 cut(s) 434
BsuRI GGCC 4 cut(s) 5, 150, 171, 820
BtsI GCAGTG 1 cut(s) 390
BtsIMutI CAGTG 3 cut(s) 45, 390, 919
BtuMI TCGCGA 1 cut(s) 859
Cac8I GCNNGC 4 cut(s) 409, 433, 654, 781
CciI TCATGA 1 cut(s) 333
Cfr13I GGNCC 3 cut(s) 387, 567, 819
Csp6I GTAC 5 cut(s) 86, 219, 604, 789, 826
CspCI CAANNNNNGTGG 2 cut(s) 21, 56
CviAII CATG 8 cut(s) 223, 284, 334, 598, 635, 649, 780, 921
CviQI GTAC 5 cut(s) 86, 219, 604, 789, 826
DdeI CTNAG 2 cut(s) 692, 723
DpnI GATC 8 cut(s) 48, 104, 233, 264, 453, 633, 676, 862
DpnII GATC 8 cut(s) 46, 102, 231, 262, 451, 631, 674, 860
DraI TTTAAA 1 cut(s) 358
Ecl136II GAGCTC 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 189
Eco47I GGWCC 2 cut(s) 387, 567
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 3 cut(s) 21, 199, 411
Eco91I GGTNACC 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 189
FaeI CATG 8 cut(s) 226, 287, 337, 601, 638, 652, 783, 924
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FatI CATG 8 cut(s) 222, 283, 333, 597, 634, 648, 779, 920
FbaI TGATCA 2 cut(s) 102, 262
Fnu4HI GCNGC 2 cut(s) 423, 918
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 2 cut(s) 423, 918
FspBI CTAG 2 cut(s) 129, 536
GluI GCNGC 2 cut(s) 423, 918
HaeIII GGCC 4 cut(s) 5, 150, 171, 820
HapII CCGG 1 cut(s) 629
Hin1II CATG 8 cut(s) 226, 287, 337, 601, 638, 652, 783, 924
HindIII AAGCTT 2 cut(s) 302, 509
HinfI GANTC 2 cut(s) 277, 544
HpaII CCGG 1 cut(s) 629
HphI GGTGA 3 cut(s) 35, 56, 325
Hpy188I TCNGA 4 cut(s) 76, 451, 543, 734
Hpy188III TCNNGA 5 cut(s) 50, 106, 334, 560, 858
HpyAV CCTTC 2 cut(s) 557, 700
HpyCH4III ACNGT 5 cut(s) 81, 491, 583, 608, 914
HpyCH4IV ACGT 1 cut(s) 787
HpyCH4V TGCA 5 cut(s) 57, 422, 457, 475, 783
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 428, 780
HpyF3I CTNAG 2 cut(s) 692, 723
HpySE526I ACGT 1 cut(s) 787
Hsp92II CATG 8 cut(s) 226, 287, 337, 601, 638, 652, 783, 924
Ksp22I TGATCA 2 cut(s) 102, 262
Kzo9I GATC 8 cut(s) 46, 102, 231, 262, 451, 631, 674, 860
LmnI GCTCC 4 cut(s) 184, 625, 734, 874
Lsp1109I GCAGC 1 cut(s) 434
MaeI CTAG 2 cut(s) 129, 536
MaeII ACGT 1 cut(s) 787
MaeIII GTNAC 1 cut(s) 269
MalI GATC 8 cut(s) 48, 104, 233, 264, 453, 633, 676, 862
MboI GATC 8 cut(s) 46, 102, 231, 262, 451, 631, 674, 860
MboII GAAGA 2 cut(s) 130, 723
MfeI CAATTG 3 cut(s) 134, 458, 465
MhlI GDGCHC 1 cut(s) 189
MlyI GAGTC 1 cut(s) 538
MmeI TCCRAC 1 cut(s) 880
MnlI CCTC 4 cut(s) 304, 558, 561, 687
MseI TTAA 5 cut(s) 174, 228, 306, 357, 876
MspA1I CMGCKG 1 cut(s) 691
MspI CCGG 1 cut(s) 629
MunI CAATTG 3 cut(s) 134, 458, 465
Mva1269I GAATGC 1 cut(s) 59
MvnI CGCG 1 cut(s) 859
MwoI GCNNNNNNNGC 3 cut(s) 156, 428, 780
NdeII GATC 8 cut(s) 46, 102, 231, 262, 451, 631, 674, 860
NlaIII CATG 8 cut(s) 226, 287, 337, 601, 638, 652, 783, 924
NlaIV GGNNCC 2 cut(s) 568, 627
NruI TCGCGA 1 cut(s) 859
NspI RCATGY 1 cut(s) 783
NspV TTCGAA 2 cut(s) 202, 806
PaeI GCATGC 1 cut(s) 783
PagI TCATGA 1 cut(s) 333
PctI GAATGC 1 cut(s) 59
PfeI GAWTC 1 cut(s) 277
PkrI GCNGC 2 cut(s) 424, 919
PleI GAGTC 1 cut(s) 538
PpsI GAGTC 1 cut(s) 538
Psp124BI GAGCTC 1 cut(s) 189
PspEI GGTNACC 1 cut(s) 269
PspN4I GGNNCC 2 cut(s) 568, 627
PspPI GGNCC 3 cut(s) 387, 567, 819
PsrI GAACNNNNNNTAC 2 cut(s) 69, 101
PvuII CAGCTG 1 cut(s) 691
RruI TCGCGA 1 cut(s) 859
RsaI GTAC 5 cut(s) 87, 220, 605, 790, 827
RsaNI GTAC 5 cut(s) 86, 219, 604, 789, 826
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 5 cut(s) 174, 228, 306, 357, 876
SatI GCNGC 2 cut(s) 423, 918
Sau3AI GATC 8 cut(s) 46, 102, 231, 262, 451, 631, 674, 860
Sau96I GGNCC 3 cut(s) 387, 567, 819
ScaI AGTACT 1 cut(s) 605
SchI GAGTC 1 cut(s) 538
SduI GDGCHC 1 cut(s) 189
SfcI CTRYAG 2 cut(s) 490, 607
SfuI TTCGAA 2 cut(s) 202, 806
SinI GGWCC 2 cut(s) 387, 567
SmlI CTYRAG 1 cut(s) 552
SmoI CTYRAG 1 cut(s) 552
SphI GCATGC 1 cut(s) 783
SsiI CCGC 4 cut(s) 495, 656, 745, 917
SspMI CTAG 2 cut(s) 129, 536
SstI GAGCTC 1 cut(s) 189
TaaI ACNGT 5 cut(s) 81, 491, 583, 608, 914
TaiI ACGT 1 cut(s) 790
TaqI TCGA 4 cut(s) 202, 438, 799, 806
TatI WGTACW 2 cut(s) 85, 603
TauI GCSGC 1 cut(s) 920
TfiI GAWTC 1 cut(s) 277
Tru1I TTAA 5 cut(s) 174, 228, 306, 357, 876
Tru9I TTAA 5 cut(s) 174, 228, 306, 357, 876
TscAI CASTG 3 cut(s) 45, 397, 919
TseI GCWGC 1 cut(s) 422
TspDTI ATGAA 5 cut(s) 322, 393, 586, 637, 699
TspRI CASTG 3 cut(s) 45, 397, 919
VpaK11BI GGWCC 2 cut(s) 387, 567
XapI RAATTY 3 cut(s) 353, 445, 499
XceI RCATGY 1 cut(s) 783
XspI CTAG 2 cut(s) 129, 536
ZrmI AGTACT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.