RchiOBHm_Chr2g0104161

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0020498
Physical Location & Seq
Forward (+)
15587972 .. 15588733
762 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47843

Sequence Viewer

Length: 630 bp
ATGGCGGACCTTGTCGCAGGGGCTGTTCTAGGAGCAGCATTTGCTGAGCTGTGTGATGGTGTTAAAAATAAGGTCATGCCCAAGAGCACGACGCCCCAACTACTCAGAGATCTGGTCTCAAAGCTAGAGTCTTTGGGACCACTGATCTCAGAGATTAAGGAGTACAACAGCTTTCTGGATCATCCGATCGACGAGGATCTAGAGGAAGTAGAACAACTTGTGGTGAATGGCAAATTGCTCATTAGCAAGTGTGCCAACTTCAAGGGTGGTCCGTGGAATAACAGAAAAAAGAATAAATACAAAAACAAACTGGAAGAACTGGACAAGTCTCTCGCCCCGTTGTTAGAAAGACTGCGAGGGCAAGCGAGGGACTTGAGAATGGAGTTGGTCAGAGCAAGGAATTCAGCGGACGCGACAGAAGTCGCGGTTGAAACTCAATCTGACATTGAAATTCAGCCTTTCTCAAGGAGGATTCAGAATTGGATGGTCAACCGAAGCTATGGTTCATGGGGCATCATGTTTATGATTCTGTTTTTGGTAAGTATGGTCATGGCAGCTACTGGTTCTACAGGCACAAAATGGTTTGTATTCAACTTTGGAATTAATTATTTAGGGATTAATAATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

209

Amino Acids

23.54

Weight (kDa)

7.7

Isoelectric Point (pI)

34.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 2 - 113 1.3e-18 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020498)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0104161
rosa_laevigata RLG00000017311
rosa_samantha Rh2AG169400 Rh2CG174900 Rh2DG174800
rosa_wichuraiana Rw2G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 413, 425
AciI CCGC 3 cut(s) 5, 407, 425
AclWI GGATC 2 cut(s) 186, 204
AcsI RAATTY 2 cut(s) 400, 450
AcyI GRCGYC 1 cut(s) 92
AfaI GTAC 1 cut(s) 164
AgsI TTSAA 4 cut(s) 262, 431, 449, 592
AluBI AGCT 5 cut(s) 49, 124, 171, 498, 557
AluI AGCT 5 cut(s) 49, 124, 171, 498, 557
Alw21I GWGCWC 1 cut(s) 89
Alw26I GTCTC 2 cut(s) 121, 333
AlwI GGATC 2 cut(s) 186, 204
AlwNI CAGNNNCTG 2 cut(s) 23, 560
ApeKI GCWGC 2 cut(s) 35, 554
ApoI RAATTY 2 cut(s) 400, 450
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AseI ATTAAT 2 cut(s) 603, 618
AspS9I GGNCC 3 cut(s) 7, 137, 269
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 3 cut(s) 7, 137, 269
Bbv12I GWGCWC 1 cut(s) 89
BbvI GCAGC 2 cut(s) 47, 566
BccI CCATC 2 cut(s) 50, 478
BcoDI GTCTC 2 cut(s) 121, 333
BfaI CTAG 3 cut(s) 29, 125, 200
BfmI CTRYAG 1 cut(s) 567
BglII AGATCT 1 cut(s) 109
BisI GCNGC 2 cut(s) 36, 555
BlpI GCTNAGC 1 cut(s) 45
BlsI GCNGC 2 cut(s) 37, 556
Bme18I GGWCC 3 cut(s) 7, 137, 269
BmgT120I GGNCC 3 cut(s) 7, 137, 269
BmiI GGNNCC 1 cut(s) 138
BmsI GCATC 1 cut(s) 522
BoxI GACNNNNGTC 1 cut(s) 419
Bpu1102I GCTNAGC 1 cut(s) 45
BpuEI CTTGAG 2 cut(s) 394, 448
BsaHI GRCGYC 1 cut(s) 92
BsaI GGTCTC 1 cut(s) 121
BsaJI CCNNGG 1 cut(s) 272
Bse1I ACTGG 3 cut(s) 315, 324, 565
BseDI CCNNGG 1 cut(s) 272
BseGI GGATG 2 cut(s) 181, 489
BseMII CTCAG 3 cut(s) 36, 118, 162
BseNI ACTGG 3 cut(s) 315, 324, 565
BseXI GCAGC 2 cut(s) 47, 566
Bsh1236I CGCG 2 cut(s) 413, 425
Bsh1285I CGRYCG 1 cut(s) 189
BsiEI CGRYCG 1 cut(s) 189
BsiHKAI GWGCWC 1 cut(s) 89
BslFI GGGAC 2 cut(s) 150, 383
BsmAI GTCTC 2 cut(s) 121, 333
BsmFI GGGAC 2 cut(s) 150, 383
Bso31I GGTCTC 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 89
Bsp143I GATC 5 cut(s) 109, 144, 178, 186, 196
Bsp1720I GCTNAGC 1 cut(s) 45
BspACI CCGC 3 cut(s) 5, 407, 425
BspCNI CTCAG 3 cut(s) 37, 117, 161
BspFNI CGCG 2 cut(s) 413, 425
BspLI GGNNCC 1 cut(s) 138
BspPI GGATC 2 cut(s) 186, 204
BspTNI GGTCTC 1 cut(s) 121
BsrI ACTGG 3 cut(s) 315, 324, 565
BssECI CCNNGG 1 cut(s) 272
BssMI GATC 5 cut(s) 109, 144, 178, 186, 196
BssNI GRCGYC 1 cut(s) 92
BstACI GRCGYC 1 cut(s) 92
BstAPI GCANNNNNTGC 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 363
BstDEI CTNAG 3 cut(s) 45, 104, 148
BstDSI CCRYGG 1 cut(s) 272
BstF5I GGATG 2 cut(s) 181, 489
BstFNI CGCG 2 cut(s) 413, 425
BstKTI GATC 5 cut(s) 112, 147, 181, 189, 199
BstMAI GTCTC 2 cut(s) 121, 333
BstMBI GATC 5 cut(s) 109, 144, 178, 186, 196
BstMCI CGRYCG 1 cut(s) 189
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstPAI GACNNNNGTC 1 cut(s) 419
BstSFI CTRYAG 1 cut(s) 567
BstUI CGCG 2 cut(s) 413, 425
BstV1I GCAGC 2 cut(s) 47, 566
BstX2I RGATCY 2 cut(s) 109, 196
BstYI RGATCY 2 cut(s) 109, 196
BtgI CCRYGG 1 cut(s) 272
BtsCI GGATG 2 cut(s) 181, 489
BtsIMutI CAGTG 1 cut(s) 140
Cac8I GCNNGC 1 cut(s) 363
CaiI CAGNNNCTG 2 cut(s) 23, 560
Cfr13I GGNCC 3 cut(s) 7, 137, 269
CseI GACGC 2 cut(s) 100, 419
Csp6I GTAC 1 cut(s) 163
CviAII CATG 4 cut(s) 76, 507, 517, 550
CviJI RGCY 7 cut(s) 23, 49, 124, 171, 457, 498, 557
CviKI_1 RGCY 7 cut(s) 23, 49, 124, 171, 457, 498, 557
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 3 cut(s) 45, 104, 148
DpnI GATC 5 cut(s) 111, 146, 180, 188, 198
DpnII GATC 5 cut(s) 109, 144, 178, 186, 196
EciI GGCGGA 1 cut(s) 20
Eco31I GGTCTC 1 cut(s) 121
Eco47I GGWCC 3 cut(s) 7, 137, 269
EcoRI GAATTC 1 cut(s) 400
FaeI CATG 4 cut(s) 79, 510, 520, 553
FaiI YATR 7 cut(s) 77, 501, 508, 518, 524, 545, 551
FaqI GGGAC 2 cut(s) 150, 383
FatI CATG 4 cut(s) 75, 506, 516, 549
Fnu4HI GCNGC 2 cut(s) 36, 555
FokI GGATG 2 cut(s) 168, 496
Fsp4HI GCNGC 2 cut(s) 36, 555
FspBI CTAG 3 cut(s) 29, 125, 200
GluI GCNGC 2 cut(s) 36, 555
HgaI GACGC 2 cut(s) 100, 419
Hin1I GRCGYC 1 cut(s) 92
Hin1II CATG 4 cut(s) 79, 510, 520, 553
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HinfI GANTC 3 cut(s) 128, 472, 526
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 1 cut(s) 490
Hpy188I TCNGA 6 cut(s) 107, 151, 186, 392, 442, 477
Hpy188III TCNNGA 2 cut(s) 176, 200
Hpy8I GTNNAC 1 cut(s) 490
Hpy99I CGWCG 2 cut(s) 94, 194
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 3 cut(s) 45, 104, 148
Hsp92I GRCGYC 1 cut(s) 92
Hsp92II CATG 4 cut(s) 79, 510, 520, 553
Kzo9I GATC 5 cut(s) 109, 144, 178, 186, 196
LmnI GCTCC 1 cut(s) 32
LpnPI CCDG 7 cut(s) 3, 98, 161, 296, 305, 546, 555
Lsp1109I GCAGC 2 cut(s) 47, 566
LweI GCATC 1 cut(s) 522
MaeI CTAG 3 cut(s) 29, 125, 200
MalI GATC 5 cut(s) 111, 146, 180, 188, 198
MboI GATC 5 cut(s) 109, 144, 178, 186, 196
MboII GAAGA 1 cut(s) 326
MflI RGATCY 2 cut(s) 109, 196
MhlI GDGCHC 1 cut(s) 89
MluCI AATT 7 cut(s) 233, 400, 450, 478, 600, 604, 622
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 5 cut(s) 187, 196, 350, 360, 462
MseI TTAA 4 cut(s) 63, 156, 603, 618
MslI CAYNNNNRTG 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 407
MvnI CGCG 2 cut(s) 413, 425
MwoI GCNNNNNNNGC 1 cut(s) 41
NdeII GATC 5 cut(s) 109, 144, 178, 186, 196
NlaIII CATG 4 cut(s) 79, 510, 520, 553
NlaIV GGNNCC 1 cut(s) 138
PfeI GAWTC 2 cut(s) 472, 526
PflFI GACNNNGTC 1 cut(s) 11
PkrI GCNGC 2 cut(s) 37, 556
Ple19I CGATCG 1 cut(s) 189
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
PshAI GACNNNNGTC 1 cut(s) 419
PshBI ATTAAT 2 cut(s) 603, 618
PspN4I GGNNCC 1 cut(s) 138
PspPI GGNCC 3 cut(s) 7, 137, 269
PstNI CAGNNNCTG 2 cut(s) 23, 560
PsuI RGATCY 2 cut(s) 109, 196
PsyI GACNNNGTC 1 cut(s) 11
PvuI CGATCG 1 cut(s) 189
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
RseI CAYNNNNRTG 1 cut(s) 521
SaqAI TTAA 4 cut(s) 63, 156, 603, 618
SatI GCNGC 2 cut(s) 36, 555
Sau3AI GATC 5 cut(s) 109, 144, 178, 186, 196
Sau96I GGNCC 3 cut(s) 7, 137, 269
SchI GAGTC 1 cut(s) 137
SduI GDGCHC 1 cut(s) 89
SetI ASST 7 cut(s) 12, 51, 75, 126, 173, 500, 559
SfaNI GCATC 1 cut(s) 522
SfcI CTRYAG 1 cut(s) 567
SinI GGWCC 3 cut(s) 7, 137, 269
SmiMI CAYNNNNRTG 1 cut(s) 521
SmlI CTYRAG 2 cut(s) 373, 463
SmoI CTYRAG 2 cut(s) 373, 463
Sse9I AATT 7 cut(s) 233, 400, 450, 478, 600, 604, 622
SsiI CCGC 3 cut(s) 5, 407, 425
SspMI CTAG 3 cut(s) 29, 125, 200
TaqI TCGA 1 cut(s) 189
TasI AATT 7 cut(s) 233, 400, 450, 478, 600, 604, 622
TatI WGTACW 1 cut(s) 162
TfiI GAWTC 2 cut(s) 472, 526
Tru1I TTAA 4 cut(s) 63, 156, 603, 618
Tru9I TTAA 4 cut(s) 63, 156, 603, 618
TscAI CASTG 1 cut(s) 147
TseI GCWGC 2 cut(s) 35, 554
TspDTI ATGAA 1 cut(s) 495
TspGWI ACGGA 1 cut(s) 261
TspRI CASTG 1 cut(s) 147
Tth111I GACNNNGTC 1 cut(s) 11
VpaK11BI GGWCC 3 cut(s) 7, 137, 269
VspI ATTAAT 2 cut(s) 603, 618
XapI RAATTY 2 cut(s) 400, 450
XbaI TCTAGA 1 cut(s) 199
XspI CTAG 3 cut(s) 29, 125, 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.