Rh2AG169400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0020498
Physical Location & Seq
Forward (+)
16051267 .. 16051941
675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG169400.1

Sequence Viewer

Length: 675 bp
ATGGCGGACCTTGTCGCAGGGGCTGTTCTAGGAGCAGCATTTGCTGAGCTGTATGATGGTGTTAAAAAGGTCATGCCCAAGAGCACGACGCCCCAACTACTCAGAGATCTGGTCTCAAAGCTAGAGTCTTTGAGACCACTGATCTCAGAGATTGAGGAGTACAACAGCTTTCTGGATCATCCGATCGACAAGGATCTAGAGGAAGTAAAACAACTTGTGGAGAATGGCAGAATGCTCATTAGCAAGTGTGCCAACTTCAAGGGTGGTCCGTGGAATAACAGAAAAAAGAATAAATACAAAAACAAACTGGAAGAATTGGACAAGTCTCTCGCCCCGTTGTTAGAAAGACTGCGACGGCAAGCGAGGGACTTGAGAATGGAGTTGTTAATGCGCCAACAGATTGAGGAACAAAGGAAGGCGAATGAGGATTCGGTCAGAGCAAGGAATTCAGCGGACGCGACAGAAGTCGCGGTTGAAACTCAATCTGACATTGAAATTCAGCCTTTCTCAAGGAGGATTCAGAATTGGATGGTCAACCGAAGCTATGGTTCATGGGGCATCATGTTTATGATTCTGTTTTTGGTAAGTATGGTCATGGCAGCTACTGGTTCTACAGGCACAAAATGGTTTGTATTCAACTTTGGAATTAATTATTTAGGGATTAATAATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

224

Amino Acids

25.71

Weight (kDa)

8.89

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 2 - 115 1.6e-21 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020498)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0104161
rosa_laevigata RLG00000017311
rosa_samantha Rh2AG169400 Rh2CG174900 Rh2DG174800
rosa_wichuraiana Rw2G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 458, 470
AciI CCGC 3 cut(s) 5, 452, 470
AclWI GGATC 2 cut(s) 183, 201
AcsI RAATTY 2 cut(s) 445, 495
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 1 cut(s) 161
AgsI TTSAA 4 cut(s) 259, 476, 494, 637
AluBI AGCT 5 cut(s) 49, 121, 168, 543, 602
AluI AGCT 5 cut(s) 49, 121, 168, 543, 602
Alw21I GWGCWC 1 cut(s) 86
Alw26I GTCTC 3 cut(s) 118, 127, 330
AlwI GGATC 2 cut(s) 183, 201
AlwNI CAGNNNCTG 2 cut(s) 23, 605
ApeKI GCWGC 2 cut(s) 35, 599
ApoI RAATTY 2 cut(s) 445, 495
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
AseI ATTAAT 2 cut(s) 648, 663
AspLEI GCGC 1 cut(s) 393
AspS9I GGNCC 2 cut(s) 7, 266
AvaII GGWCC 2 cut(s) 7, 266
Bbv12I GWGCWC 1 cut(s) 86
BbvI GCAGC 2 cut(s) 47, 611
BccI CCATC 2 cut(s) 50, 523
BceAI ACGGC 1 cut(s) 371
BcoDI GTCTC 3 cut(s) 118, 127, 330
BfaI CTAG 3 cut(s) 29, 122, 197
BfmI CTRYAG 1 cut(s) 612
BglII AGATCT 1 cut(s) 106
BisI GCNGC 2 cut(s) 36, 600
BlpI GCTNAGC 1 cut(s) 45
BlsI GCNGC 2 cut(s) 37, 601
Bme18I GGWCC 2 cut(s) 7, 266
BmgT120I GGNCC 2 cut(s) 7, 266
BmsI GCATC 1 cut(s) 567
BoxI GACNNNNGTC 1 cut(s) 464
Bpu1102I GCTNAGC 1 cut(s) 45
BpuEI CTTGAG 2 cut(s) 391, 493
BsaHI GRCGYC 1 cut(s) 89
BsaI GGTCTC 2 cut(s) 118, 127
BsaJI CCNNGG 1 cut(s) 269
Bse1I ACTGG 2 cut(s) 312, 610
BseDI CCNNGG 1 cut(s) 269
BseGI GGATG 2 cut(s) 178, 534
BseMII CTCAG 3 cut(s) 36, 115, 159
BseNI ACTGG 2 cut(s) 312, 610
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 2 cut(s) 47, 611
Bsh1236I CGCG 2 cut(s) 458, 470
Bsh1285I CGRYCG 1 cut(s) 186
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 1 cut(s) 86
BslFI GGGAC 1 cut(s) 380
BsmAI GTCTC 3 cut(s) 118, 127, 330
BsmFI GGGAC 1 cut(s) 380
BsmI GAATGC 1 cut(s) 237
Bso31I GGTCTC 2 cut(s) 118, 127
Bsp1286I GDGCHC 1 cut(s) 86
Bsp143I GATC 5 cut(s) 106, 141, 175, 183, 193
Bsp1720I GCTNAGC 1 cut(s) 45
BspACI CCGC 3 cut(s) 5, 452, 470
BspCNI CTCAG 3 cut(s) 37, 114, 158
BspFNI CGCG 2 cut(s) 458, 470
BspPI GGATC 2 cut(s) 183, 201
BspTNI GGTCTC 2 cut(s) 118, 127
BsrI ACTGG 2 cut(s) 312, 610
BssECI CCNNGG 1 cut(s) 269
BssMI GATC 5 cut(s) 106, 141, 175, 183, 193
BssNI GRCGYC 1 cut(s) 89
BstACI GRCGYC 1 cut(s) 89
BstAPI GCANNNNNTGC 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 3 cut(s) 45, 101, 145
BstDSI CCRYGG 1 cut(s) 269
BstF5I GGATG 2 cut(s) 178, 534
BstFNI CGCG 2 cut(s) 458, 470
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 5 cut(s) 109, 144, 178, 186, 196
BstMAI GTCTC 3 cut(s) 118, 127, 330
BstMBI GATC 5 cut(s) 106, 141, 175, 183, 193
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstPAI GACNNNNGTC 1 cut(s) 464
BstSFI CTRYAG 1 cut(s) 612
BstUI CGCG 2 cut(s) 458, 470
BstV1I GCAGC 2 cut(s) 47, 611
BstX2I RGATCY 2 cut(s) 106, 193
BstYI RGATCY 2 cut(s) 106, 193
BtgI CCRYGG 1 cut(s) 269
BtsCI GGATG 2 cut(s) 178, 534
BtsIMutI CAGTG 1 cut(s) 137
Cac8I GCNNGC 1 cut(s) 360
CaiI CAGNNNCTG 2 cut(s) 23, 605
CfoI GCGC 1 cut(s) 393
Cfr13I GGNCC 2 cut(s) 7, 266
CseI GACGC 2 cut(s) 97, 464
Csp6I GTAC 1 cut(s) 160
CviAII CATG 4 cut(s) 73, 552, 562, 595
CviJI RGCY 7 cut(s) 23, 49, 121, 168, 502, 543, 602
CviKI_1 RGCY 7 cut(s) 23, 49, 121, 168, 502, 543, 602
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 3 cut(s) 45, 101, 145
DpnI GATC 5 cut(s) 108, 143, 177, 185, 195
DpnII GATC 5 cut(s) 106, 141, 175, 183, 193
EciI GGCGGA 1 cut(s) 20
Eco31I GGTCTC 2 cut(s) 118, 127
Eco47I GGWCC 2 cut(s) 7, 266
EcoRI GAATTC 1 cut(s) 445
FaeI CATG 4 cut(s) 76, 555, 565, 598
FaiI YATR 8 cut(s) 54, 74, 546, 553, 563, 569, 590, 596
FaqI GGGAC 1 cut(s) 380
FatI CATG 4 cut(s) 72, 551, 561, 594
Fnu4HI GCNGC 2 cut(s) 36, 600
FokI GGATG 2 cut(s) 165, 541
Fsp4HI GCNGC 2 cut(s) 36, 600
FspBI CTAG 3 cut(s) 29, 122, 197
GlaI GCGC 1 cut(s) 392
GluI GCNGC 2 cut(s) 36, 600
HgaI GACGC 2 cut(s) 97, 464
HhaI GCGC 1 cut(s) 393
Hin1I GRCGYC 1 cut(s) 89
Hin1II CATG 4 cut(s) 76, 555, 565, 598
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HinfI GANTC 4 cut(s) 125, 428, 517, 571
Hpy166II GTNNAC 1 cut(s) 535
Hpy188I TCNGA 6 cut(s) 104, 148, 183, 437, 487, 522
Hpy188III TCNNGA 2 cut(s) 173, 197
Hpy8I GTNNAC 1 cut(s) 535
Hpy99I CGWCG 2 cut(s) 91, 357
HpyAV CCTTC 1 cut(s) 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 3 cut(s) 45, 101, 145
Hsp92I GRCGYC 1 cut(s) 89
Hsp92II CATG 4 cut(s) 76, 555, 565, 598
HspAI GCGC 1 cut(s) 391
Kzo9I GATC 5 cut(s) 106, 141, 175, 183, 193
LmnI GCTCC 1 cut(s) 32
LpnPI CCDG 6 cut(s) 3, 95, 158, 293, 591, 600
Lsp1109I GCAGC 2 cut(s) 47, 611
LweI GCATC 1 cut(s) 567
MaeI CTAG 3 cut(s) 29, 122, 197
MalI GATC 5 cut(s) 108, 143, 177, 185, 195
MboI GATC 5 cut(s) 106, 141, 175, 183, 193
MboII GAAGA 1 cut(s) 323
MflI RGATCY 2 cut(s) 106, 193
MhlI GDGCHC 1 cut(s) 86
MluCI AATT 7 cut(s) 314, 445, 495, 523, 645, 649, 667
MlyI GAGTC 1 cut(s) 134
MnlI CCTC 6 cut(s) 148, 193, 357, 397, 418, 507
MseI TTAA 4 cut(s) 63, 386, 648, 663
MslI CAYNNNNRTG 1 cut(s) 566
MspA1I CMGCKG 1 cut(s) 452
Mva1269I GAATGC 1 cut(s) 237
MvnI CGCG 2 cut(s) 458, 470
MwoI GCNNNNNNNGC 1 cut(s) 41
NdeII GATC 5 cut(s) 106, 141, 175, 183, 193
NlaIII CATG 4 cut(s) 76, 555, 565, 598
PctI GAATGC 1 cut(s) 237
PfeI GAWTC 3 cut(s) 428, 517, 571
PflFI GACNNNGTC 1 cut(s) 11
PkrI GCNGC 2 cut(s) 37, 601
Ple19I CGATCG 1 cut(s) 186
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
PshAI GACNNNNGTC 1 cut(s) 464
PshBI ATTAAT 2 cut(s) 648, 663
PspPI GGNCC 2 cut(s) 7, 266
PstNI CAGNNNCTG 2 cut(s) 23, 605
PsuI RGATCY 2 cut(s) 106, 193
PsyI GACNNNGTC 1 cut(s) 11
PvuI CGATCG 1 cut(s) 186
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
RseI CAYNNNNRTG 1 cut(s) 566
SaqAI TTAA 4 cut(s) 63, 386, 648, 663
SatI GCNGC 2 cut(s) 36, 600
Sau3AI GATC 5 cut(s) 106, 141, 175, 183, 193
Sau96I GGNCC 2 cut(s) 7, 266
SchI GAGTC 1 cut(s) 134
SduI GDGCHC 1 cut(s) 86
SetI ASST 7 cut(s) 12, 51, 72, 123, 170, 545, 604
SfaNI GCATC 1 cut(s) 567
SfcI CTRYAG 1 cut(s) 612
SinI GGWCC 2 cut(s) 7, 266
SmiMI CAYNNNNRTG 1 cut(s) 566
SmlI CTYRAG 2 cut(s) 370, 508
SmoI CTYRAG 2 cut(s) 370, 508
Sse9I AATT 7 cut(s) 314, 445, 495, 523, 645, 649, 667
SsiI CCGC 3 cut(s) 5, 452, 470
SspMI CTAG 3 cut(s) 29, 122, 197
TaqI TCGA 1 cut(s) 186
TaqII GACCGA 1 cut(s) 421
TasI AATT 7 cut(s) 314, 445, 495, 523, 645, 649, 667
TatI WGTACW 1 cut(s) 159
TfiI GAWTC 3 cut(s) 428, 517, 571
Tru1I TTAA 4 cut(s) 63, 386, 648, 663
Tru9I TTAA 4 cut(s) 63, 386, 648, 663
TscAI CASTG 1 cut(s) 144
TseI GCWGC 2 cut(s) 35, 599
TspDTI ATGAA 1 cut(s) 540
TspGWI ACGGA 1 cut(s) 258
TspRI CASTG 1 cut(s) 144
Tth111I GACNNNGTC 1 cut(s) 11
VpaK11BI GGWCC 2 cut(s) 7, 266
VspI ATTAAT 2 cut(s) 648, 663
XapI RAATTY 2 cut(s) 445, 495
XbaI TCTAGA 1 cut(s) 196
XspI CTAG 3 cut(s) 29, 122, 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.