Rw2G013250

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0020498
Physical Location & Seq
Forward (+)
13997488 .. 14005564
8077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013250.1

Sequence Viewer

Length: 588 bp
ATGGCGGCCGCCCTTATCGGAGAGGATGCTGTAGTAGCAGAAGGAATTGCTGTGCTGTGTGATGGTGTTAAGCAGGTCATGCCCAAGAGCGAGACGCCCCAAGTATTCAGAGATCTGGTTTCAAAGCTAGAGTCTTTGGGACCACTGATCTCTGAGATTAATTACAACAGCTCTCTGGATCATCCGATCGACAAGGATCTAGAGGAAGTAAAACAACTTGTGGTGAATGGCATGAGGCTCATTCGCAAGTGTGCCAACTTCAAGGGTGGTCCGTGGAATAGCAGAAAAAAGAATAAATACAAAAACAAACTGGAAGAATTGGACAAGTCTCTCGCCCCGTTGTTAGAAAGACTGCGAGGGCAAGCGAGGGACTTGAGAATGGAGTTGTTAATGCGCCAACAGATTGTGGAACAAAGGAAGGCGAATGAGGCTTTGGTCAGAGCAAGGAATTCAGCGGACGCGACAGAAGTCGCGGCTTTGGTCGGCGGCGGTGGGGGCTTCTTCTCATCGTCGATCAGGCCTGCAAGTGCGGCGGCGAAGGTGGCGCTACGGCGGAAGATCGATGCGTCGATAGTGGCTGCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

195

Amino Acids

21.26

Weight (kDa)

9.51

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 23 - 115 2.6e-11 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020498)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0104161
rosa_laevigata RLG00000017311
rosa_samantha Rh2AG169400 Rh2CG174900 Rh2DG174800
rosa_wichuraiana Rw2G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 64
AccII CGCG 2 cut(s) 461, 473
AciI CCGC 9 cut(s) 5, 9, 455, 473, 486, 489, 530, 533, 553
AclWI GGATC 2 cut(s) 186, 204
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 1 cut(s) 448
AcyI GRCGYC 1 cut(s) 95
AgsI TTSAA 2 cut(s) 123, 262
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AluBI AGCT 2 cut(s) 127, 171
AluI AGCT 2 cut(s) 127, 171
Alw26I GTCTC 2 cut(s) 86, 333
AlwI GGATC 2 cut(s) 186, 204
AoxI GGCC 2 cut(s) 6, 518
ApeKI GCWGC 1 cut(s) 578
ApoI RAATTY 1 cut(s) 448
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
AseI ATTAAT 1 cut(s) 159
AspLEI GCGC 2 cut(s) 396, 547
AspS9I GGNCC 2 cut(s) 140, 269
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 2 cut(s) 140, 269
BbvI GCAGC 1 cut(s) 565
BccI CCATC 1 cut(s) 56
BceAI ACGGC 1 cut(s) 566
BcoDI GTCTC 2 cut(s) 86, 333
BfaI CTAG 2 cut(s) 128, 200
BfmI CTRYAG 1 cut(s) 30
BfoI RGCGCY 1 cut(s) 548
BfuAI ACCTGC 1 cut(s) 64
BglII AGATCT 1 cut(s) 112
BisI GCNGC 7 cut(s) 6, 9, 474, 487, 531, 534, 579
BlsI GCNGC 7 cut(s) 7, 10, 475, 488, 532, 535, 580
Bme18I GGWCC 2 cut(s) 140, 269
BmgT120I GGNCC 2 cut(s) 140, 269
BmiI GGNNCC 1 cut(s) 141
BmsI GCATC 2 cut(s) 16, 553
BoxI GACNNNNGTC 1 cut(s) 467
BpuEI CTTGAG 1 cut(s) 394
Bsa29I ATCGAT 1 cut(s) 561
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 272
Bse1I ACTGG 1 cut(s) 315
BseCI ATCGAT 1 cut(s) 561
BseDI CCNNGG 1 cut(s) 272
BseGI GGATG 2 cut(s) 31, 181
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 1 cut(s) 315
BseX3I CGGCCG 1 cut(s) 6
BseXI GCAGC 1 cut(s) 565
BsgI GTGCAG 1 cut(s) 564
Bsh1236I CGCG 2 cut(s) 461, 473
Bsh1285I CGRYCG 2 cut(s) 9, 189
BshFI GGCC 2 cut(s) 8, 520
BshVI ATCGAT 1 cut(s) 561
BsiEI CGRYCG 2 cut(s) 9, 189
BslFI GGGAC 2 cut(s) 153, 383
BsmAI GTCTC 2 cut(s) 86, 333
BsmBI CGTCTC 1 cut(s) 86
BsmFI GGGAC 2 cut(s) 153, 383
BsnI GGCC 2 cut(s) 8, 520
Bsp143I GATC 7 cut(s) 112, 147, 178, 186, 196, 513, 558
BspACI CCGC 9 cut(s) 5, 9, 455, 473, 486, 489, 530, 533, 553
BspANI GGCC 2 cut(s) 8, 520
BspCNI CTCAG 1 cut(s) 145
BspDI ATCGAT 1 cut(s) 561
BspFNI CGCG 2 cut(s) 461, 473
BspLI GGNNCC 1 cut(s) 141
BspMI ACCTGC 1 cut(s) 64
BspPI GGATC 2 cut(s) 186, 204
BsrI ACTGG 1 cut(s) 315
BssECI CCNNGG 1 cut(s) 272
BssMI GATC 7 cut(s) 112, 147, 178, 186, 196, 513, 558
BssNI GRCGYC 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 585
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 79
BstC8I GCNNGC 2 cut(s) 363, 522
BstDEI CTNAG 1 cut(s) 153
BstDSI CCRYGG 1 cut(s) 272
BstF5I GGATG 2 cut(s) 31, 181
BstFNI CGCG 2 cut(s) 461, 473
BstH2I RGCGCY 1 cut(s) 548
BstHHI GCGC 2 cut(s) 396, 547
BstKTI GATC 7 cut(s) 115, 150, 181, 189, 199, 516, 561
BstMAI GTCTC 2 cut(s) 86, 333
BstMBI GATC 7 cut(s) 112, 147, 178, 186, 196, 513, 558
BstMCI CGRYCG 2 cut(s) 9, 189
BstMWI GCNNNNNNNGC 6 cut(s) 35, 79, 428, 495, 530, 542
BstPAI GACNNNNGTC 1 cut(s) 467
BstSFI CTRYAG 1 cut(s) 30
BstUI CGCG 2 cut(s) 461, 473
BstV1I GCAGC 1 cut(s) 565
BstX2I RGATCY 2 cut(s) 112, 196
BstYI RGATCY 2 cut(s) 112, 196
BstZI CGGCCG 1 cut(s) 6
Bsu15I ATCGAT 1 cut(s) 561
BsuRI GGCC 2 cut(s) 8, 520
BsuTUI ATCGAT 1 cut(s) 561
BtgI CCRYGG 1 cut(s) 272
BtsCI GGATG 2 cut(s) 31, 181
BtsIMutI CAGTG 2 cut(s) 143, 581
BveI ACCTGC 1 cut(s) 64
Cac8I GCNNGC 2 cut(s) 363, 522
CciNI GCGGCCGC 1 cut(s) 6
CfoI GCGC 2 cut(s) 396, 547
Cfr13I GGNCC 2 cut(s) 140, 269
ClaI ATCGAT 1 cut(s) 561
CseI GACGC 3 cut(s) 103, 467, 555
CviAII CATG 2 cut(s) 79, 232
CviJI RGCY 9 cut(s) 8, 127, 171, 238, 431, 476, 498, 520, 578
CviKI_1 RGCY 9 cut(s) 8, 127, 171, 238, 431, 476, 498, 520, 578
DdeI CTNAG 1 cut(s) 153
DpnI GATC 7 cut(s) 114, 149, 180, 188, 198, 515, 560
DpnII GATC 7 cut(s) 112, 147, 178, 186, 196, 513, 558
EaeI YGGCCR 1 cut(s) 6
EagI CGGCCG 1 cut(s) 6
EciI GGCGGA 1 cut(s) 568
EclXI CGGCCG 1 cut(s) 6
Eco147I AGGCCT 1 cut(s) 520
Eco47I GGWCC 2 cut(s) 140, 269
Eco52I CGGCCG 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 448
Esp3I CGTCTC 1 cut(s) 86
FaeI CATG 2 cut(s) 82, 235
FaiI YATR 2 cut(s) 80, 233
FaqI GGGAC 2 cut(s) 153, 383
FatI CATG 2 cut(s) 78, 231
Fnu4HI GCNGC 7 cut(s) 6, 9, 474, 487, 531, 534, 579
FokI GGATG 2 cut(s) 38, 168
Fsp4HI GCNGC 7 cut(s) 6, 9, 474, 487, 531, 534, 579
FspBI CTAG 2 cut(s) 128, 200
GlaI GCGC 2 cut(s) 395, 546
GluI GCNGC 7 cut(s) 6, 9, 474, 487, 531, 534, 579
HaeII RGCGCY 1 cut(s) 548
HaeIII GGCC 2 cut(s) 8, 520
HgaI GACGC 3 cut(s) 103, 467, 555
HhaI GCGC 2 cut(s) 396, 547
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 2 cut(s) 82, 235
Hin6I GCGC 2 cut(s) 394, 545
HinP1I GCGC 2 cut(s) 394, 545
HinfI GANTC 1 cut(s) 131
HphI GGTGA 1 cut(s) 235
Hpy188I TCNGA 5 cut(s) 20, 110, 154, 186, 440
Hpy188III TCNNGA 2 cut(s) 176, 200
Hpy99I CGWCG 2 cut(s) 514, 571
HpyAV CCTTC 3 cut(s) 35, 412, 532
HpyCH4III ACNGT 1 cut(s) 585
HpyCH4V TGCA 2 cut(s) 524, 581
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 79, 428, 495, 530, 542
HpyF3I CTNAG 1 cut(s) 153
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 2 cut(s) 82, 235
HspAI GCGC 2 cut(s) 394, 545
Kzo9I GATC 7 cut(s) 112, 147, 178, 186, 196, 513, 558
LpnPI CCDG 6 cut(s) 59, 101, 161, 296, 502, 534
Lsp1109I GCAGC 1 cut(s) 565
LweI GCATC 2 cut(s) 16, 553
MaeI CTAG 2 cut(s) 128, 200
MalI GATC 7 cut(s) 114, 149, 180, 188, 198, 515, 560
MboI GATC 7 cut(s) 112, 147, 178, 186, 196, 513, 558
MboII GAAGA 3 cut(s) 326, 493, 568
MflI RGATCY 2 cut(s) 112, 196
MluCI AATT 4 cut(s) 45, 160, 317, 448
MlyI GAGTC 1 cut(s) 140
MnlI CCTC 6 cut(s) 16, 196, 228, 350, 360, 421
MseI TTAA 3 cut(s) 69, 159, 389
MspA1I CMGCKG 1 cut(s) 455
MvnI CGCG 2 cut(s) 461, 473
MwoI GCNNNNNNNGC 6 cut(s) 35, 79, 428, 495, 530, 542
NdeII GATC 7 cut(s) 112, 147, 178, 186, 196, 513, 558
NlaIII CATG 2 cut(s) 82, 235
NlaIV GGNNCC 1 cut(s) 141
NotI GCGGCCGC 1 cut(s) 6
PceI AGGCCT 1 cut(s) 520
PkrI GCNGC 7 cut(s) 7, 10, 475, 488, 532, 535, 580
Ple19I CGATCG 1 cut(s) 189
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PshAI GACNNNNGTC 1 cut(s) 467
PshBI ATTAAT 1 cut(s) 159
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 2 cut(s) 140, 269
PsuI RGATCY 2 cut(s) 112, 196
PvuI CGATCG 1 cut(s) 189
SaqAI TTAA 3 cut(s) 69, 159, 389
SatI GCNGC 7 cut(s) 6, 9, 474, 487, 531, 534, 579
Sau3AI GATC 7 cut(s) 112, 147, 178, 186, 196, 513, 558
Sau96I GGNCC 2 cut(s) 140, 269
SchI GAGTC 1 cut(s) 140
SetI ASST 4 cut(s) 78, 129, 173, 543
SfaNI GCATC 2 cut(s) 16, 553
SfcI CTRYAG 1 cut(s) 30
SinI GGWCC 2 cut(s) 140, 269
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 4 cut(s) 45, 160, 317, 448
SseBI AGGCCT 1 cut(s) 520
SsiI CCGC 9 cut(s) 5, 9, 455, 473, 486, 489, 530, 533, 553
SspMI CTAG 2 cut(s) 128, 200
StuI AGGCCT 1 cut(s) 520
TaaI ACNGT 1 cut(s) 585
TaqI TCGA 4 cut(s) 189, 512, 561, 569
TasI AATT 4 cut(s) 45, 160, 317, 448
TauI GCSGC 6 cut(s) 8, 11, 476, 489, 533, 536
Tru1I TTAA 3 cut(s) 69, 159, 389
Tru9I TTAA 3 cut(s) 69, 159, 389
TscAI CASTG 2 cut(s) 150, 588
TseI GCWGC 1 cut(s) 578
TspGWI ACGGA 1 cut(s) 261
TspRI CASTG 2 cut(s) 150, 588
VpaK11BI GGWCC 2 cut(s) 140, 269
VspI ATTAAT 1 cut(s) 159
XapI RAATTY 1 cut(s) 448
XbaI TCTAGA 1 cut(s) 199
XspI CTAG 2 cut(s) 128, 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.