RLG00000017311

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0020498
Physical Location & Seq
Forward (+)
15135457 .. 15136437
981 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017311

Sequence Viewer

Length: 702 bp
ATGGCGGCCGCCCTTATCGGAGAGGCTGCTGTAGTAGCAGCAGCAATGGCTGTGCTGTGTGATGGTGTTAAGGAGGTCATGCTCAAGAGCAAGACGCCCCAACTATTCAGAGATCTGGTTTCAAAGCTAGAGTCTTTGGGACCACTGATCTCTGAGATTGAGGAGTACAAGAGCTTTCTGGATCATCCGATCGACAAGGATCTGGAGGAAGTAAAACAACTTGTGGAGAATGGCATAAGGCTCATTCGCAAGTGTGCCAACTTCAAGGGTGGTCCGTGGAATAGCAGAAAAAATAATAAATACAAAAACAAACTGGAAGAGTTGGACAAGTCTCTCGCCCCGTTGTTAGAAAGACTGCGAGGGCAAGCGAGGGACTTGAGAATGGAGTTGTTAATGCGCCGACAGATTGAGGAACAAAGGAAGGCTAATGAGGCTTTGGTCAGAGCAAGGAATTCGGCGGACGCGGCAGAAGTCGCGGTTGAAACTCAATCTGACATTGAAATTCAGCCCTTCTCAAGGAGGATTCAGAATTGGATGGTCAACCAAAGCTATGGTTCATGGGGAATCATGTTTATGATTCTGTTTTTGGCTTTGGTCGGCGGCGGTGGGGGCTTCTTCTCATCGTCGATCAGGCCTGCAAGTGCGGCGGCGAAGGTGGTGCTACGGCGGAAGATCGATGCGTCGATAGTGGCTGCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

234

Amino Acids

25.94

Weight (kDa)

9.34

Isoelectric Point (pI)

48.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 10 - 117 1.9e-14 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020498)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0104161
rosa_laevigata RLG00000017311
rosa_samantha Rh2AG169400 Rh2CG174900 Rh2DG174800
rosa_wichuraiana Rw2G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 464, 476
AclWI GGATC 2 cut(s) 189, 207
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 2 cut(s) 451, 501
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 1 cut(s) 167
AfiI CCNNNNNNNGG 1 cut(s) 516
AgsI TTSAA 4 cut(s) 123, 265, 482, 500
AluBI AGCT 3 cut(s) 127, 174, 549
AluI AGCT 3 cut(s) 127, 174, 549
Alw26I GTCTC 1 cut(s) 336
AlwI GGATC 2 cut(s) 189, 207
AoxI GGCC 2 cut(s) 6, 632
ApeKI GCWGC 4 cut(s) 26, 38, 41, 692
ApoI RAATTY 2 cut(s) 451, 501
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
AspLEI GCGC 1 cut(s) 399
AspS9I GGNCC 2 cut(s) 140, 272
AvaII GGWCC 2 cut(s) 140, 272
BbvI GCAGC 4 cut(s) 13, 50, 53, 679
BccI CCATC 2 cut(s) 56, 529
BceAI ACGGC 1 cut(s) 680
BcgI CGANNNNNNTGC 4 cut(s) 435, 469, 640, 674
BcoDI GTCTC 1 cut(s) 336
BfaI CTAG 1 cut(s) 128
BfmI CTRYAG 1 cut(s) 30
BglII AGATCT 1 cut(s) 112
Bme18I GGWCC 2 cut(s) 140, 272
BmgT120I GGNCC 2 cut(s) 140, 272
BmiI GGNNCC 1 cut(s) 141
BmsI GCATC 1 cut(s) 667
BpmI CTGGAG 1 cut(s) 224
BpuEI CTTGAG 3 cut(s) 68, 397, 499
Bsa29I ATCGAT 1 cut(s) 675
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 275
Bsc4I CCNNNNNNNGG 1 cut(s) 516
Bse1I ACTGG 1 cut(s) 318
Bse3DI GCAATG 1 cut(s) 51
BseCI ATCGAT 1 cut(s) 675
BseDI CCNNGG 1 cut(s) 275
BseGI GGATG 2 cut(s) 184, 540
BseLI CCNNNNNNNGG 1 cut(s) 516
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 1 cut(s) 318
BseRI GAGGAG 1 cut(s) 176
BseX3I CGGCCG 1 cut(s) 6
BseXI GCAGC 4 cut(s) 13, 50, 53, 679
BsgI GTGCAG 1 cut(s) 678
Bsh1236I CGCG 2 cut(s) 464, 476
Bsh1285I CGRYCG 2 cut(s) 9, 192
BshFI GGCC 2 cut(s) 8, 634
BshVI ATCGAT 1 cut(s) 675
BsiEI CGRYCG 2 cut(s) 9, 192
BslFI GGGAC 2 cut(s) 153, 386
BslI CCNNNNNNNGG 1 cut(s) 516
BsmAI GTCTC 1 cut(s) 336
BsmFI GGGAC 2 cut(s) 153, 386
BsnI GGCC 2 cut(s) 8, 634
Bsp143I GATC 7 cut(s) 112, 147, 181, 189, 199, 627, 672
BspANI GGCC 2 cut(s) 8, 634
BspCNI CTCAG 1 cut(s) 145
BspDI ATCGAT 1 cut(s) 675
BspFNI CGCG 2 cut(s) 464, 476
BspLI GGNNCC 1 cut(s) 141
BspPI GGATC 2 cut(s) 189, 207
BsrDI GCAATG 1 cut(s) 51
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 1 cut(s) 275
BssMI GATC 7 cut(s) 112, 147, 181, 189, 199, 627, 672
BssNI GRCGYC 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 699
Bst6I CTCTTC 1 cut(s) 312
BstACI GRCGYC 1 cut(s) 95
BstC8I GCNNGC 2 cut(s) 366, 636
BstDEI CTNAG 1 cut(s) 153
BstDSI CCRYGG 1 cut(s) 275
BstENI CCTNNNNNAGG 1 cut(s) 514
BstF5I GGATG 2 cut(s) 184, 540
BstFNI CGCG 2 cut(s) 464, 476
BstHHI GCGC 1 cut(s) 399
BstKTI GATC 7 cut(s) 115, 150, 184, 192, 202, 630, 675
BstMAI GTCTC 1 cut(s) 336
BstMBI GATC 7 cut(s) 112, 147, 181, 189, 199, 627, 672
BstMCI CGRYCG 2 cut(s) 9, 192
BstMWI GCNNNNNNNGC 7 cut(s) 35, 47, 431, 464, 473, 609, 644
BstSFI CTRYAG 1 cut(s) 30
BstUI CGCG 2 cut(s) 464, 476
BstV1I GCAGC 4 cut(s) 13, 50, 53, 679
BstX2I RGATCY 2 cut(s) 112, 199
BstXI CCANNNNNNTGG 1 cut(s) 551
BstYI RGATCY 2 cut(s) 112, 199
BstZI CGGCCG 1 cut(s) 6
Bsu15I ATCGAT 1 cut(s) 675
BsuRI GGCC 2 cut(s) 8, 634
BsuTUI ATCGAT 1 cut(s) 675
BtgI CCRYGG 1 cut(s) 275
BtsCI GGATG 2 cut(s) 184, 540
BtsIMutI CAGTG 2 cut(s) 143, 695
Cac8I GCNNGC 2 cut(s) 366, 636
CciNI GCGGCCGC 1 cut(s) 6
CfoI GCGC 1 cut(s) 399
Cfr13I GGNCC 2 cut(s) 140, 272
ClaI ATCGAT 1 cut(s) 675
CseI GACGC 3 cut(s) 103, 470, 669
Csp6I GTAC 1 cut(s) 166
CviAII CATG 3 cut(s) 79, 558, 568
CviQI GTAC 1 cut(s) 166
DdeI CTNAG 1 cut(s) 153
DpnI GATC 7 cut(s) 114, 149, 183, 191, 201, 629, 674
DpnII GATC 7 cut(s) 112, 147, 181, 189, 199, 627, 672
EaeI YGGCCR 1 cut(s) 6
EagI CGGCCG 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
EciI GGCGGA 2 cut(s) 473, 682
EclXI CGGCCG 1 cut(s) 6
Eco147I AGGCCT 1 cut(s) 634
Eco47I GGWCC 2 cut(s) 140, 272
Eco52I CGGCCG 1 cut(s) 6
EcoNI CCTNNNNNAGG 1 cut(s) 514
EcoRI GAATTC 1 cut(s) 451
FaeI CATG 3 cut(s) 82, 561, 571
FaiI YATR 6 cut(s) 80, 236, 552, 559, 569, 575
FaqI GGGAC 2 cut(s) 153, 386
FatI CATG 3 cut(s) 78, 557, 567
FokI GGATG 2 cut(s) 171, 547
FspBI CTAG 1 cut(s) 128
GlaI GCGC 1 cut(s) 398
GsuI CTGGAG 1 cut(s) 224
HaeIII GGCC 2 cut(s) 8, 634
HgaI GACGC 3 cut(s) 103, 470, 669
HhaI GCGC 1 cut(s) 399
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 3 cut(s) 82, 561, 571
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 4 cut(s) 131, 523, 564, 577
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 7 cut(s) 20, 110, 154, 189, 443, 493, 528
Hpy188III TCNNGA 3 cut(s) 85, 179, 203
Hpy8I GTNNAC 1 cut(s) 541
Hpy99I CGWCG 2 cut(s) 628, 685
HpyAV CCTTC 3 cut(s) 415, 520, 646
HpyCH4III ACNGT 1 cut(s) 699
HpyCH4V TGCA 2 cut(s) 638, 695
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 47, 431, 464, 473, 609, 644
HpyF3I CTNAG 1 cut(s) 153
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 3 cut(s) 82, 561, 571
HspAI GCGC 1 cut(s) 397
Kzo9I GATC 7 cut(s) 112, 147, 181, 189, 199, 627, 672
LpnPI CCDG 6 cut(s) 101, 164, 188, 299, 616, 648
Lsp1109I GCAGC 4 cut(s) 13, 50, 53, 679
LweI GCATC 1 cut(s) 667
MaeI CTAG 1 cut(s) 128
MalI GATC 7 cut(s) 114, 149, 183, 191, 201, 629, 674
MboI GATC 7 cut(s) 112, 147, 181, 189, 199, 627, 672
MboII GAAGA 3 cut(s) 329, 607, 682
MflI RGATCY 2 cut(s) 112, 199
MluCI AATT 3 cut(s) 451, 501, 529
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 9 cut(s) 16, 67, 154, 199, 353, 363, 403, 424, 513
MseI TTAA 2 cut(s) 69, 392
MslI CAYNNNNRTG 1 cut(s) 572
MvnI CGCG 2 cut(s) 464, 476
MwoI GCNNNNNNNGC 7 cut(s) 35, 47, 431, 464, 473, 609, 644
NdeII GATC 7 cut(s) 112, 147, 181, 189, 199, 627, 672
NlaIII CATG 3 cut(s) 82, 561, 571
NlaIV GGNNCC 1 cut(s) 141
NotI GCGGCCGC 1 cut(s) 6
PceI AGGCCT 1 cut(s) 634
PfeI GAWTC 3 cut(s) 523, 564, 577
Ple19I CGATCG 1 cut(s) 192
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 2 cut(s) 140, 272
PsuI RGATCY 2 cut(s) 112, 199
PvuI CGATCG 1 cut(s) 192
RsaI GTAC 1 cut(s) 167
RsaNI GTAC 1 cut(s) 166
RseI CAYNNNNRTG 1 cut(s) 572
SaqAI TTAA 2 cut(s) 69, 392
Sau3AI GATC 7 cut(s) 112, 147, 181, 189, 199, 627, 672
Sau96I GGNCC 2 cut(s) 140, 272
SchI GAGTC 1 cut(s) 140
SetI ASST 5 cut(s) 78, 129, 176, 551, 657
SfaNI GCATC 1 cut(s) 667
SfcI CTRYAG 1 cut(s) 30
SinI GGWCC 2 cut(s) 140, 272
SmiMI CAYNNNNRTG 1 cut(s) 572
SmlI CTYRAG 3 cut(s) 83, 376, 514
SmoI CTYRAG 3 cut(s) 83, 376, 514
Sse9I AATT 3 cut(s) 451, 501, 529
SseBI AGGCCT 1 cut(s) 634
SspMI CTAG 1 cut(s) 128
StuI AGGCCT 1 cut(s) 634
TaaI ACNGT 1 cut(s) 699
TaqI TCGA 4 cut(s) 192, 626, 675, 683
TasI AATT 3 cut(s) 451, 501, 529
TatI WGTACW 1 cut(s) 165
TauI GCSGC 6 cut(s) 8, 11, 467, 603, 647, 650
TfiI GAWTC 3 cut(s) 523, 564, 577
Tru1I TTAA 2 cut(s) 69, 392
Tru9I TTAA 2 cut(s) 69, 392
TscAI CASTG 2 cut(s) 150, 702
TseI GCWGC 4 cut(s) 26, 38, 41, 692
TspDTI ATGAA 1 cut(s) 546
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 2 cut(s) 150, 702
VpaK11BI GGWCC 2 cut(s) 140, 272
XagI CCTNNNNNAGG 1 cut(s) 514
XapI RAATTY 2 cut(s) 451, 501
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.