RchiOBHm_Chr2g0111111

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0018567
Physical Location & Seq
Forward (+)
22738024 .. 22738911
888 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48472

Sequence Viewer

Length: 888 bp
ATGTTTGGTCGCCGACGAAAAGCATTGAAAATCTTCAAATCTCTTAGAGAAATTTTTCAATTGCACCAGCGACAACACCGACGAGCAGAAAAACAATGCGGTCCTAACACCTTGAGGGAGTACACGGCCGAAGAGATTAAATTAGCTACTCATGATTTTTCTGACCCGGTATTACTTCAAGCTCAAGGCATGTGGAGCAGTGTGTTCATAGCTCCAATTAAAGACGAAACAGTTGCAATCAAGTTTCTTGCACAGGAATATTGCTTCGAAGACATGGTTGAATCCCTCGGCAAGATTAAACACCCTAATATTCTTGCCATGAAAGGCATATGCTCCGAGCTGCGGTGCTTTGTTTATGAGTATATGGAGAATTGCTGCTTCGATGACTTCAAAAGAAACATTCCATGGCAAGACCGCATCCGTATTGCTGCGGATGTTTGCTCGGCTTTGTGCTACCTTTACCAGCTTCAGCCTGGATTTATAGTTCAGGGTCATCTCAGTGCATCCAACGTCCTCATCGATAGCGAAATCTCTGCCAAGGTTAATCTTGAGCAGCCTGAAGTGTACCGGACAAGCGAATTGGTAAAGGATGTGAAGGCTTTTGGGAAGTTGATTCTGCATCTATTAGGTGGATATGAGGGGACTAGTACTGTTACTTATTATTTGTCTGAAGCCTATGTCCAGCTTCGGTTTCTAGACGAGACAGCTGGTCAATGGCCGATGGAATTGGTTGAGGAACTTCTGAAGTTGTCTGAAAGGTGTACTTCCTTTCAGGGATGGGATGATAATCTTAGAGAACCGTATATGGTGATAGTAATGAAAGAGCTTAAATTGATTAAGGAGAAAGCTGACCAAATTGCCAGTCATGCAAGAGAGAACTCGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr U-box

Protein Analysis

295

Amino Acids

34.2

Weight (kDa)

6.03

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 67 - 181 1.1e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018567)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0111111
rosa_laevigata RLG00000017865
rosa_multiflora Rmu_sc0012949.1_g000002
rosa_roxburghii Rroxscaffold_2G00132700
rosa_rugosa Rorug02G0170900
rosa_samantha Rh2AG223900 Rh2BG235500 Rh2CG227000 Rh2DG231500
rosa_wichuraiana Rw2G017270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 99, 343, 415, 431
AcoI YGGCCR 2 cut(s) 126, 716
AcsI RAATTY 1 cut(s) 51
AcuI CTGAAG 4 cut(s) 452, 579, 690, 764
AfaI GTAC 4 cut(s) 122, 566, 649, 763
AfiI CCNNNNNNNGG 1 cut(s) 342
AgsI TTSAA 6 cut(s) 28, 37, 59, 179, 281, 391
AhdI GACNNNNNGTC 1 cut(s) 708
AhlI ACTAGT 1 cut(s) 644
AjnI CCWGG 1 cut(s) 472
AloI GAACNNNNNNTCC 2 cut(s) 468, 500
AluBI AGCT 9 cut(s) 146, 182, 212, 340, 466, 685, 707, 826, 848
AluI AGCT 9 cut(s) 146, 182, 212, 340, 466, 685, 707, 826, 848
Alw26I GTCTC 1 cut(s) 695
AoxI GGCC 2 cut(s) 126, 716
ApeKI GCWGC 4 cut(s) 340, 375, 428, 553
ApoI RAATTY 1 cut(s) 51
ArsI GACNNNNNNTTYG 2 cut(s) 847, 879
Asp700I GAANNNNTTC 2 cut(s) 32, 54
AspS9I GGNCC 1 cut(s) 101
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 820
AsuII TTCGAA 1 cut(s) 267
AvaII GGWCC 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 276
BbvI GCAGC 4 cut(s) 327, 362, 415, 565
BccI CCATC 2 cut(s) 715, 771
BceAI ACGGC 1 cut(s) 141
BcgI CGANNNNNNTGC 4 cut(s) 215, 249, 515, 549
BciT130I CCWGG 1 cut(s) 474
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 695
BcuI ACTAGT 1 cut(s) 644
BfaI CTAG 2 cut(s) 645, 695
BisI GCNGC 4 cut(s) 341, 376, 429, 554
BlsI GCNGC 4 cut(s) 342, 377, 430, 555
BmcAI AGTACT 1 cut(s) 649
Bme1390I CCNGG 2 cut(s) 167, 474
Bme18I GGWCC 1 cut(s) 101
BmeRI GACNNNNNGTC 1 cut(s) 708
BmgT120I GGNCC 1 cut(s) 101
BmrFI CCNGG 2 cut(s) 167, 474
BmsI GCATC 3 cut(s) 426, 512, 628
BpiI GAAGAC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 3 cut(s) 133, 168, 569
BpuMI CCSGG 1 cut(s) 167
Bsa29I ATCGAT 1 cut(s) 519
BsaBI GATNNNNATC 1 cut(s) 786
BsaJI CCNNGG 3 cut(s) 286, 404, 537
BsaWI WCCGGW 1 cut(s) 567
Bsc4I CCNNNNNNNGG 1 cut(s) 342
Bse1I ACTGG 1 cut(s) 861
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 1 cut(s) 474
BseCI ATCGAT 1 cut(s) 519
BseDI CCNNGG 3 cut(s) 286, 404, 537
BseGI GGATG 6 cut(s) 417, 439, 503, 595, 782, 787
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 1 cut(s) 342
BseMII CTCAG 1 cut(s) 511
BseNI ACTGG 1 cut(s) 861
BseX3I CGGCCG 1 cut(s) 126
BseXI GCAGC 4 cut(s) 327, 362, 415, 565
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 2 cut(s) 128, 718
BshVI ATCGAT 1 cut(s) 519
BsiEI CGRYCG 1 cut(s) 129
BsiSI CCGG 2 cut(s) 167, 568
BslFI GGGAC 1 cut(s) 655
BslI CCNNNNNNNGG 1 cut(s) 342
BsmAI GTCTC 1 cut(s) 695
BsmFI GGGAC 1 cut(s) 655
BsnI GGCC 2 cut(s) 128, 718
Bsp119I TTCGAA 1 cut(s) 267
Bsp19I CCATGG 1 cut(s) 404
BspACI CCGC 4 cut(s) 99, 343, 415, 431
BspANI GGCC 2 cut(s) 128, 718
BspCNI CTCAG 1 cut(s) 510
BspDI ATCGAT 1 cut(s) 519
BspHI TCATGA 1 cut(s) 151
BspT104I TTCGAA 1 cut(s) 267
BsrI ACTGG 1 cut(s) 861
BssECI CCNNGG 3 cut(s) 286, 404, 537
BssT1I CCWWGG 2 cut(s) 404, 537
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 3 cut(s) 232, 652, 801
Bst6I CTCTTC 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 267
BstDEI CTNAG 3 cut(s) 44, 497, 791
BstDSI CCRYGG 1 cut(s) 404
BstF5I GGATG 6 cut(s) 417, 439, 503, 595, 782, 787
BstMAI GTCTC 1 cut(s) 695
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 2 cut(s) 195, 866
BstNI CCWGG 1 cut(s) 474
BstNSI RCATGY 1 cut(s) 193
BstSCI CCNGG 2 cut(s) 165, 472
BstV1I GCAGC 4 cut(s) 327, 362, 415, 565
BstV2I GAAGAC 1 cut(s) 276
BstZI CGGCCG 1 cut(s) 126
Bsu15I ATCGAT 1 cut(s) 519
BsuRI GGCC 2 cut(s) 128, 718
BsuTUI ATCGAT 1 cut(s) 519
BtgI CCRYGG 1 cut(s) 404
BtsCI GGATG 6 cut(s) 417, 439, 503, 595, 782, 787
BtsI GCAGTG 1 cut(s) 205
BtsIMutI CAGTG 2 cut(s) 205, 505
CciI TCATGA 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 101
ClaI ATCGAT 1 cut(s) 519
Csp6I GTAC 4 cut(s) 121, 565, 648, 762
CspCI CAANNNNNGTGG 2 cut(s) 173, 208
CviAII CATG 6 cut(s) 152, 190, 274, 319, 405, 866
CviQI GTAC 4 cut(s) 121, 565, 648, 762
DdeI CTNAG 3 cut(s) 44, 497, 791
DriI GACNNNNNGTC 1 cut(s) 708
EaeI YGGCCR 2 cut(s) 126, 716
EagI CGGCCG 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 126
Eam1105I GACNNNNNGTC 1 cut(s) 708
EarI CTCTTC 1 cut(s) 126
EclXI CGGCCG 1 cut(s) 126
Eco130I CCWWGG 2 cut(s) 404, 537
Eco47I GGWCC 1 cut(s) 101
Eco52I CGGCCG 1 cut(s) 126
Eco57I CTGAAG 4 cut(s) 452, 579, 690, 764
EcoRII CCWGG 1 cut(s) 472
EcoT14I CCWWGG 2 cut(s) 404, 537
ErhI CCWWGG 2 cut(s) 404, 537
FaeI CATG 6 cut(s) 155, 193, 277, 322, 408, 869
FalI AAGNNNNNCTT 2 cut(s) 748, 780
FaqI GGGAC 1 cut(s) 655
FatI CATG 6 cut(s) 151, 189, 273, 318, 404, 865
FauNDI CATATG 1 cut(s) 329
Fnu4HI GCNGC 4 cut(s) 341, 376, 429, 554
FokI GGATG 6 cut(s) 404, 446, 490, 602, 789, 794
Fsp4HI GCNGC 4 cut(s) 341, 376, 429, 554
FspBI CTAG 2 cut(s) 645, 695
GluI GCNGC 4 cut(s) 341, 376, 429, 554
HaeIII GGCC 2 cut(s) 128, 718
HapII CCGG 2 cut(s) 167, 568
Hin1II CATG 6 cut(s) 155, 193, 277, 322, 408, 869
HinfI GANTC 2 cut(s) 281, 613
HpaII CCGG 2 cut(s) 167, 568
HphI GGTGA 1 cut(s) 820
Hpy166II GTNNAC 3 cut(s) 123, 565, 762
Hpy188I TCNGA 5 cut(s) 163, 337, 670, 744, 754
Hpy188III TCNNGA 3 cut(s) 152, 548, 695
Hpy8I GTNNAC 3 cut(s) 123, 565, 762
Hpy99I CGWCG 2 cut(s) 18, 84
HpyAV CCTTC 1 cut(s) 589
HpyCH4III ACNGT 3 cut(s) 232, 652, 801
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 6 cut(s) 64, 236, 251, 503, 619, 869
HpyF10VI GCNNNNNNNGC 2 cut(s) 195, 866
HpyF3I CTNAG 3 cut(s) 44, 497, 791
HpySE526I ACGT 1 cut(s) 510
Hsp92II CATG 6 cut(s) 155, 193, 277, 322, 408, 869
LmnI GCTCC 3 cut(s) 195, 217, 338
Lsp1109I GCAGC 4 cut(s) 327, 362, 415, 565
LweI GCATC 3 cut(s) 426, 512, 628
MaeI CTAG 2 cut(s) 645, 695
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 1 cut(s) 652
MboII GAAGA 3 cut(s) 25, 143, 281
MfeI CAATTG 1 cut(s) 59
MluCI AATT 9 cut(s) 51, 59, 140, 216, 370, 578, 725, 830, 855
MmeI TCCRAC 1 cut(s) 531
MnlI CCTC 5 cut(s) 108, 296, 524, 631, 727
MroXI GAANNNNTTC 2 cut(s) 32, 54
MseI TTAA 6 cut(s) 138, 219, 297, 543, 828, 837
MslI CAYNNNNRTG 1 cut(s) 498
MspA1I CMGCKG 1 cut(s) 707
MspI CCGG 2 cut(s) 167, 568
MspR9I CCNGG 2 cut(s) 167, 474
MunI CAATTG 1 cut(s) 59
MvaI CCWGG 1 cut(s) 474
MwoI GCNNNNNNNGC 2 cut(s) 195, 866
NciI CCSGG 1 cut(s) 167
NcoI CCATGG 1 cut(s) 404
NdeI CATATG 1 cut(s) 329
NlaIII CATG 6 cut(s) 155, 193, 277, 322, 408, 869
NmeAIII GCCGAG 2 cut(s) 267, 422
NspI RCATGY 1 cut(s) 193
NspV TTCGAA 1 cut(s) 267
PagI TCATGA 1 cut(s) 151
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PdmI GAANNNNTTC 2 cut(s) 32, 54
PfeI GAWTC 2 cut(s) 281, 613
PkrI GCNGC 4 cut(s) 342, 377, 430, 555
Psp6I CCWGG 1 cut(s) 472
PspGI CCWGG 1 cut(s) 472
PspPI GGNCC 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 707
RsaI GTAC 4 cut(s) 122, 566, 649, 763
RsaNI GTAC 4 cut(s) 121, 565, 648, 762
RseI CAYNNNNRTG 1 cut(s) 498
SaqAI TTAA 6 cut(s) 138, 219, 297, 543, 828, 837
SatI GCNGC 4 cut(s) 341, 376, 429, 554
Sau96I GGNCC 1 cut(s) 101
ScaI AGTACT 1 cut(s) 649
ScrFI CCNGG 2 cut(s) 167, 474
SfaNI GCATC 3 cut(s) 426, 512, 628
SfuI TTCGAA 1 cut(s) 267
SinI GGWCC 1 cut(s) 101
SmiMI CAYNNNNRTG 1 cut(s) 498
SmlI CTYRAG 3 cut(s) 112, 183, 548
SmoI CTYRAG 3 cut(s) 112, 183, 548
SpeI ACTAGT 1 cut(s) 644
Sse9I AATT 9 cut(s) 51, 59, 140, 216, 370, 578, 725, 830, 855
SsiI CCGC 4 cut(s) 99, 343, 415, 431
SspI AATATT 2 cut(s) 260, 310
SspMI CTAG 2 cut(s) 645, 695
StyD4I CCNGG 2 cut(s) 165, 472
StyI CCWWGG 2 cut(s) 404, 537
TaaI ACNGT 3 cut(s) 232, 652, 801
TaiI ACGT 1 cut(s) 513
TaqI TCGA 3 cut(s) 267, 381, 519
TasI AATT 9 cut(s) 51, 59, 140, 216, 370, 578, 725, 830, 855
TatI WGTACW 3 cut(s) 120, 647, 761
TfiI GAWTC 2 cut(s) 281, 613
Tru1I TTAA 6 cut(s) 138, 219, 297, 543, 828, 837
Tru9I TTAA 6 cut(s) 138, 219, 297, 543, 828, 837
TscAI CASTG 2 cut(s) 205, 505
TseI GCWGC 4 cut(s) 340, 375, 428, 553
TspDTI ATGAA 3 cut(s) 196, 335, 833
TspGWI ACGGA 1 cut(s) 410
TspRI CASTG 2 cut(s) 205, 505
VpaK11BI GGWCC 1 cut(s) 101
XapI RAATTY 1 cut(s) 51
XbaI TCTAGA 1 cut(s) 694
XceI RCATGY 1 cut(s) 193
XcmI CCANNNNNNNNNTGG 1 cut(s) 470
XmnI GAANNNNTTC 2 cut(s) 32, 54
XspI CTAG 2 cut(s) 645, 695
ZrmI AGTACT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.