Rroxscaffold_2G00132700

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018567
Physical Location & Seq
Reverse (-)
69694421 .. 69695119
699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00132700.1

Sequence Viewer

Length: 699 bp
ATGTGGAGCAGTGTGTTCATAGCTCCAATTAAAGACGAAACAGTTGCAATCAAGTTTCTTGCACAGGAATATTGCTTCGAAGACATGGTTGAATCCCTCGGCAAGATTAAACACCCTAATATTCTTGCCATGAAAGGCATATGCTCCGAGCTGCGGTGCTTTGTTTATGAGTATATGGAGAATAGCTGCTTCGATGACTTCAAAAGAAACATTTCATGGCAAGACCGCATCCTTATTGCTGCGGATGTTTGTTCGGCTTTGTGCTACCTTTACCAGCTTCAGCCTGGATTTATAGTTCATGGTCACCTCAGTGCATCCAACGTCTTCATCGATAGCGAAATCTCTGCCAAGGTTAATCTTGAGCAGCCTGAAGTGTACCGGACAAGCGAATTGGTAAAGGATGTGAAGGCTTTTGGGAAGTTGATTCTGCATCTATTAGGTGGATATGAGGGGACTAGTACTGTTACTTACTATTTGTCTGAAACCTATGTCCAGCTTCGGTTTCTAGACGAGACAGCTGGTCAATGGCCGATGGAATTGGTTGAGGAACTTCTGAAGTTGTCTGAAAGGTGTACTTCCTTTCAGGGATGGGATGATAATCTTAGAGAACCGTATATGGTGATAGTAATGAAAGAGCTTAAATTGATTAAGGAGAAAGCTGACCAAATTGCCAGTCATGCAAGACAGAACTCGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr U-box

Protein Analysis

232

Amino Acids

26.73

Weight (kDa)

5.03

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 12 - 118 4.9e-12 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 12 - 117 1.2e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018567)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0111111
rosa_laevigata RLG00000017865
rosa_multiflora Rmu_sc0012949.1_g000002
rosa_roxburghii Rroxscaffold_2G00132700
rosa_rugosa Rorug02G0170900
rosa_samantha Rh2AG223900 Rh2BG235500 Rh2CG227000 Rh2DG231500
rosa_wichuraiana Rw2G017270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 154, 226, 242
AcoI YGGCCR 1 cut(s) 527
AcuI CTGAAG 3 cut(s) 263, 390, 575
AfaI GTAC 3 cut(s) 377, 460, 574
AfiI CCNNNNNNNGG 1 cut(s) 153
AgsI TTSAA 2 cut(s) 92, 202
AhdI GACNNNNNGTC 1 cut(s) 519
AhlI ACTAGT 1 cut(s) 455
AjnI CCWGG 1 cut(s) 283
AleI CACNNNNGTG 1 cut(s) 309
AloI GAACNNNNNNTCC 2 cut(s) 279, 311
AluBI AGCT 8 cut(s) 23, 151, 186, 277, 496, 518, 637, 659
AluI AGCT 8 cut(s) 23, 151, 186, 277, 496, 518, 637, 659
Alw26I GTCTC 1 cut(s) 506
AoxI GGCC 1 cut(s) 527
ApeKI GCWGC 4 cut(s) 151, 186, 239, 364
ArsI GACNNNNNNTTYG 2 cut(s) 658, 690
Asp700I GAANNNNTTC 1 cut(s) 211
AsuHPI GGTGA 2 cut(s) 296, 631
AsuII TTCGAA 1 cut(s) 78
BbsI GAAGAC 2 cut(s) 87, 316
BbvI GCAGC 4 cut(s) 138, 173, 226, 376
BccI CCATC 2 cut(s) 526, 582
BcgI CGANNNNNNTGC 4 cut(s) 26, 60, 326, 360
BciT130I CCWGG 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 506
BcuI ACTAGT 1 cut(s) 455
BfaI CTAG 2 cut(s) 456, 506
BisI GCNGC 4 cut(s) 152, 187, 240, 365
BlsI GCNGC 4 cut(s) 153, 188, 241, 366
BmcAI AGTACT 1 cut(s) 460
Bme1390I CCNGG 1 cut(s) 285
BmeRI GACNNNNNGTC 1 cut(s) 519
BmrFI CCNGG 1 cut(s) 285
BmsI GCATC 3 cut(s) 237, 323, 439
BpiI GAAGAC 2 cut(s) 87, 316
Bpu14I TTCGAA 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 380
Bsa29I ATCGAT 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 597
BsaJI CCNNGG 2 cut(s) 97, 348
BsaWI WCCGGW 1 cut(s) 378
Bsc4I CCNNNNNNNGG 1 cut(s) 153
Bse1I ACTGG 1 cut(s) 672
Bse8I GATNNNNATC 1 cut(s) 597
BseBI CCWGG 1 cut(s) 285
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 2 cut(s) 97, 348
BseGI GGATG 6 cut(s) 228, 250, 314, 406, 593, 598
BseJI GATNNNNATC 1 cut(s) 597
BseLI CCNNNNNNNGG 1 cut(s) 153
BseMII CTCAG 1 cut(s) 322
BseNI ACTGG 1 cut(s) 672
BseXI GCAGC 4 cut(s) 138, 173, 226, 376
BshFI GGCC 1 cut(s) 529
BshVI ATCGAT 1 cut(s) 330
BsiSI CCGG 1 cut(s) 379
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 1 cut(s) 153
BsmAI GTCTC 1 cut(s) 506
BsmFI GGGAC 1 cut(s) 466
BsnI GGCC 1 cut(s) 529
Bsp119I TTCGAA 1 cut(s) 78
BspACI CCGC 3 cut(s) 154, 226, 242
BspANI GGCC 1 cut(s) 529
BspCNI CTCAG 1 cut(s) 321
BspDI ATCGAT 1 cut(s) 330
BspT104I TTCGAA 1 cut(s) 78
BsrI ACTGG 1 cut(s) 672
BssECI CCNNGG 2 cut(s) 97, 348
BssT1I CCWWGG 1 cut(s) 348
Bst2UI CCWGG 1 cut(s) 285
Bst4CI ACNGT 3 cut(s) 43, 463, 612
BstBI TTCGAA 1 cut(s) 78
BstDEI CTNAG 2 cut(s) 308, 602
BstEII GGTNACC 1 cut(s) 302
BstF5I GGATG 6 cut(s) 228, 250, 314, 406, 593, 598
BstMAI GTCTC 1 cut(s) 506
BstMWI GCNNNNNNNGC 1 cut(s) 677
BstNI CCWGG 1 cut(s) 285
BstPI GGTNACC 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 283
BstV1I GCAGC 4 cut(s) 138, 173, 226, 376
BstV2I GAAGAC 2 cut(s) 87, 316
Bsu15I ATCGAT 1 cut(s) 330
BsuRI GGCC 1 cut(s) 529
BsuTUI ATCGAT 1 cut(s) 330
BtsCI GGATG 6 cut(s) 228, 250, 314, 406, 593, 598
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 2 cut(s) 16, 316
ClaI ATCGAT 1 cut(s) 330
Csp6I GTAC 3 cut(s) 376, 459, 573
CviAII CATG 5 cut(s) 85, 130, 216, 299, 677
CviQI GTAC 3 cut(s) 376, 459, 573
DdeI CTNAG 2 cut(s) 308, 602
DriI GACNNNNNGTC 1 cut(s) 519
EaeI YGGCCR 1 cut(s) 527
Eam1105I GACNNNNNGTC 1 cut(s) 519
Eco130I CCWWGG 1 cut(s) 348
Eco57I CTGAAG 3 cut(s) 263, 390, 575
Eco91I GGTNACC 1 cut(s) 302
EcoO65I GGTNACC 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 5 cut(s) 88, 133, 219, 302, 680
FalI AAGNNNNNCTT 2 cut(s) 559, 591
FaqI GGGAC 1 cut(s) 466
FatI CATG 5 cut(s) 84, 129, 215, 298, 676
FauNDI CATATG 1 cut(s) 140
Fnu4HI GCNGC 4 cut(s) 152, 187, 240, 365
FokI GGATG 6 cut(s) 215, 257, 301, 413, 600, 605
Fsp4HI GCNGC 4 cut(s) 152, 187, 240, 365
FspBI CTAG 2 cut(s) 456, 506
GluI GCNGC 4 cut(s) 152, 187, 240, 365
HaeIII GGCC 1 cut(s) 529
HapII CCGG 1 cut(s) 379
Hin1II CATG 5 cut(s) 88, 133, 219, 302, 680
HinfI GANTC 2 cut(s) 92, 424
HpaII CCGG 1 cut(s) 379
HphI GGTGA 2 cut(s) 296, 631
Hpy166II GTNNAC 2 cut(s) 376, 573
Hpy188I TCNGA 4 cut(s) 148, 481, 555, 565
Hpy188III TCNNGA 2 cut(s) 359, 506
Hpy8I GTNNAC 2 cut(s) 376, 573
HpyAV CCTTC 1 cut(s) 400
HpyCH4III ACNGT 3 cut(s) 43, 463, 612
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 5 cut(s) 47, 62, 314, 430, 680
HpyF10VI GCNNNNNNNGC 1 cut(s) 677
HpyF3I CTNAG 2 cut(s) 308, 602
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 5 cut(s) 88, 133, 219, 302, 680
LmnI GCTCC 3 cut(s) 6, 28, 149
Lsp1109I GCAGC 4 cut(s) 138, 173, 226, 376
LweI GCATC 3 cut(s) 237, 323, 439
MaeI CTAG 2 cut(s) 456, 506
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 2 cut(s) 302, 463
MboII GAAGA 2 cut(s) 92, 316
MluCI AATT 5 cut(s) 27, 389, 536, 641, 666
MmeI TCCRAC 1 cut(s) 342
MnlI CCTC 4 cut(s) 107, 317, 442, 538
MroXI GAANNNNTTC 1 cut(s) 211
MseI TTAA 5 cut(s) 30, 108, 354, 639, 648
MslI CAYNNNNRTG 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 1 cut(s) 285
MvaI CCWGG 1 cut(s) 285
MwoI GCNNNNNNNGC 1 cut(s) 677
NdeI CATATG 1 cut(s) 140
NlaIII CATG 5 cut(s) 88, 133, 219, 302, 680
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 302
NspV TTCGAA 1 cut(s) 78
OliI CACNNNNGTG 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 2 cut(s) 92, 424
PkrI GCNGC 4 cut(s) 153, 188, 241, 366
Psp6I CCWGG 1 cut(s) 283
PspEI GGTNACC 1 cut(s) 302
PspGI CCWGG 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 3 cut(s) 377, 460, 574
RsaNI GTAC 3 cut(s) 376, 459, 573
RseI CAYNNNNRTG 1 cut(s) 309
SaqAI TTAA 5 cut(s) 30, 108, 354, 639, 648
SatI GCNGC 4 cut(s) 152, 187, 240, 365
ScaI AGTACT 1 cut(s) 460
ScrFI CCNGG 1 cut(s) 285
SfaNI GCATC 3 cut(s) 237, 323, 439
SfuI TTCGAA 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 309
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
SpeI ACTAGT 1 cut(s) 455
Sse9I AATT 5 cut(s) 27, 389, 536, 641, 666
SsiI CCGC 3 cut(s) 154, 226, 242
SspI AATATT 2 cut(s) 71, 121
SspMI CTAG 2 cut(s) 456, 506
StyD4I CCNGG 1 cut(s) 283
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 3 cut(s) 43, 463, 612
TaiI ACGT 1 cut(s) 324
TaqI TCGA 3 cut(s) 78, 192, 330
TasI AATT 5 cut(s) 27, 389, 536, 641, 666
TatI WGTACW 2 cut(s) 458, 572
TfiI GAWTC 2 cut(s) 92, 424
Tru1I TTAA 5 cut(s) 30, 108, 354, 639, 648
Tru9I TTAA 5 cut(s) 30, 108, 354, 639, 648
TscAI CASTG 2 cut(s) 16, 316
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 4 cut(s) 151, 186, 239, 364
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 6 cut(s) 7, 146, 204, 287, 316, 644
TspRI CASTG 2 cut(s) 16, 316
XbaI TCTAGA 1 cut(s) 505
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 2 cut(s) 456, 506
ZrmI AGTACT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.