RLG00000017865

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018567
Physical Location & Seq
Forward (+)
22456179 .. 22457069
891 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017865

Sequence Viewer

Length: 891 bp
ATGTTTGGTCGTCGACTAAAAGCATTGAAAATCTTCAAATCTCTTAGAGAAATTTTTCAACTGCACCGGCGACAACACCGACGAGCAGAAAAGCAATGCGGTCCTAACACCTTGAGGGAGTACACGGCTGAAGAGATTAAATTAGCTACTCATGATTTTTCTGACCCGGTATTACTTCAAGCTCAAGGCATGTGGAGCAGTGTGTTCATAGCTCCAATTAAAGACGAAACAGTTGCAATCAAGTTTCTTGCACAGGAATATTGCTTCGAAGACATGGTTGAATCCCTCGGCAAGATTAAACACCCTAATATTCTTGCCATGAAAGGCATATGCTCCGAGCTGCGGTGCTTTGTTTATGAGTATATGGAGAATAGCTGCTTCGATGACTTCAAAAGAAAAATTTCATGGCAAGACCGCATCCTTATTGCTGCGGATGTTTGCTCGGCTTTGTGCTACCTTTACCAGCTTCTGCCTGGATTTATAGTGCAGGGTCATCTCAGTGCATCCAACGTCCTCATCGATAGCGAAATCTCTGCCAAGGTTAATCTTGAGCAGCCTGAAGTGTACCGGACAAGCGAATTGGTAAAGGATGTGAAGGCTTTTGGGAAGTTGATTCTGCATCTATTAGGTGGATATGAGGGGACTAGTACTGTTACTTATTATTTGTCTGAAACCTATGTCCAGCTTCGGTTTCTAGACGAGACAGCTGGTCAATGGCCGATGGAATTGGTTGAGGATCTTCTGAAGTTGTCTGAAAGGTGTACTTCCTTTCAGGGATGGGATGATAATCTTAGAGAACCGTATATGGTGATTGTAATGAAAGAGCTTAAATTGATTAAGGAGAAAGCTGACCAAATTGCCAGTCATCCAAGAGAGAACTCGCAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr U-box

Protein Analysis

297

Amino Acids

34.28

Weight (kDa)

5.89

Isoelectric Point (pI)

36.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 67 - 181 2.3e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018567)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0111111
rosa_laevigata RLG00000017865
rosa_multiflora Rmu_sc0012949.1_g000002
rosa_roxburghii Rroxscaffold_2G00132700
rosa_rugosa Rorug02G0170900
rosa_samantha Rh2AG223900 Rh2BG235500 Rh2CG227000 Rh2DG231500
rosa_wichuraiana Rw2G017270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 13
AciI CCGC 4 cut(s) 99, 343, 415, 431
AclWI GGATC 1 cut(s) 744
AcoI YGGCCR 1 cut(s) 716
AcsI RAATTY 2 cut(s) 51, 399
AcuI CTGAAG 3 cut(s) 150, 579, 764
AfaI GTAC 4 cut(s) 122, 566, 649, 763
AfiI CCNNNNNNNGG 1 cut(s) 342
AgsI TTSAA 6 cut(s) 28, 37, 59, 179, 281, 391
AhdI GACNNNNNGTC 1 cut(s) 708
AhlI ACTAGT 1 cut(s) 644
AjnI CCWGG 1 cut(s) 472
Alw26I GTCTC 1 cut(s) 695
AlwI GGATC 1 cut(s) 744
AlwNI CAGNNNCTG 1 cut(s) 469
AoxI GGCC 1 cut(s) 716
ApeKI GCWGC 4 cut(s) 340, 375, 428, 553
ApoI RAATTY 2 cut(s) 51, 399
ArsI GACNNNNNNTTYG 2 cut(s) 847, 879
Asp700I GAANNNNTTC 3 cut(s) 32, 54, 400
AspS9I GGNCC 1 cut(s) 101
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 820
AsuII TTCGAA 1 cut(s) 267
AvaII GGWCC 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 276
BbvI GCAGC 4 cut(s) 327, 362, 415, 565
BccI CCATC 2 cut(s) 715, 771
BceAI ACGGC 1 cut(s) 141
BcgI CGANNNNNNTGC 4 cut(s) 215, 249, 515, 549
BciT130I CCWGG 1 cut(s) 474
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 695
BcuI ACTAGT 1 cut(s) 644
BfaI CTAG 2 cut(s) 645, 695
BisI GCNGC 4 cut(s) 341, 376, 429, 554
BlsI GCNGC 4 cut(s) 342, 377, 430, 555
BmcAI AGTACT 1 cut(s) 649
Bme1390I CCNGG 2 cut(s) 167, 474
Bme18I GGWCC 1 cut(s) 101
BmeRI GACNNNNNGTC 1 cut(s) 708
BmgT120I GGNCC 1 cut(s) 101
BmrFI CCNGG 2 cut(s) 167, 474
BmsI GCATC 3 cut(s) 426, 512, 628
BpiI GAAGAC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 3 cut(s) 133, 168, 569
BpuMI CCSGG 1 cut(s) 167
Bsa29I ATCGAT 1 cut(s) 519
BsaBI GATNNNNATC 1 cut(s) 786
BsaJI CCNNGG 2 cut(s) 286, 537
BsaWI WCCGGW 1 cut(s) 567
Bsc4I CCNNNNNNNGG 1 cut(s) 342
Bse118I RCCGGY 1 cut(s) 66
Bse1I ACTGG 1 cut(s) 861
Bse3DI GCAATG 1 cut(s) 101
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 1 cut(s) 474
BseCI ATCGAT 1 cut(s) 519
BseDI CCNNGG 2 cut(s) 286, 537
BseGI GGATG 7 cut(s) 417, 439, 503, 595, 782, 787, 865
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 1 cut(s) 342
BseMI GCAATG 1 cut(s) 101
BseMII CTCAG 1 cut(s) 511
BseNI ACTGG 1 cut(s) 861
BseXI GCAGC 4 cut(s) 327, 362, 415, 565
BsgI GTGCAG 2 cut(s) 47, 506
BshFI GGCC 1 cut(s) 718
BshVI ATCGAT 1 cut(s) 519
BsiSI CCGG 3 cut(s) 67, 167, 568
BslFI GGGAC 1 cut(s) 655
BslI CCNNNNNNNGG 1 cut(s) 342
BsmAI GTCTC 1 cut(s) 695
BsmFI GGGAC 1 cut(s) 655
BsnI GGCC 1 cut(s) 718
Bsp119I TTCGAA 1 cut(s) 267
Bsp143I GATC 1 cut(s) 736
BspACI CCGC 4 cut(s) 99, 343, 415, 431
BspANI GGCC 1 cut(s) 718
BspCNI CTCAG 1 cut(s) 510
BspDI ATCGAT 1 cut(s) 519
BspHI TCATGA 1 cut(s) 151
BspPI GGATC 1 cut(s) 744
BspT104I TTCGAA 1 cut(s) 267
BsrDI GCAATG 1 cut(s) 101
BsrFI RCCGGY 1 cut(s) 66
BsrI ACTGG 1 cut(s) 861
BssAI RCCGGY 1 cut(s) 66
BssECI CCNNGG 2 cut(s) 286, 537
BssMI GATC 1 cut(s) 736
BssT1I CCWWGG 1 cut(s) 537
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 3 cut(s) 232, 652, 801
Bst6I CTCTTC 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 267
BstDEI CTNAG 3 cut(s) 44, 497, 791
BstF5I GGATG 7 cut(s) 417, 439, 503, 595, 782, 787, 865
BstKTI GATC 1 cut(s) 739
BstMAI GTCTC 1 cut(s) 695
BstMBI GATC 1 cut(s) 736
BstMWI GCNNNNNNNGC 1 cut(s) 195
BstNI CCWGG 1 cut(s) 474
BstNSI RCATGY 1 cut(s) 193
BstSCI CCNGG 2 cut(s) 165, 472
BstV1I GCAGC 4 cut(s) 327, 362, 415, 565
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 1 cut(s) 736
BstYI RGATCY 1 cut(s) 736
Bsu15I ATCGAT 1 cut(s) 519
BsuRI GGCC 1 cut(s) 718
BsuTUI ATCGAT 1 cut(s) 519
BtsCI GGATG 7 cut(s) 417, 439, 503, 595, 782, 787, 865
BtsI GCAGTG 1 cut(s) 205
BtsIMutI CAGTG 2 cut(s) 205, 505
CaiI CAGNNNCTG 1 cut(s) 469
CciI TCATGA 1 cut(s) 151
Cfr10I RCCGGY 1 cut(s) 66
Cfr13I GGNCC 1 cut(s) 101
ClaI ATCGAT 1 cut(s) 519
Csp6I GTAC 4 cut(s) 121, 565, 648, 762
CspCI CAANNNNNGTGG 2 cut(s) 173, 208
CviAII CATG 5 cut(s) 152, 190, 274, 319, 405
CviQI GTAC 4 cut(s) 121, 565, 648, 762
DdeI CTNAG 3 cut(s) 44, 497, 791
DpnI GATC 1 cut(s) 738
DpnII GATC 1 cut(s) 736
DriI GACNNNNNGTC 1 cut(s) 708
EaeI YGGCCR 1 cut(s) 716
Eam1104I CTCTTC 1 cut(s) 126
Eam1105I GACNNNNNGTC 1 cut(s) 708
EarI CTCTTC 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 537
Eco47I GGWCC 1 cut(s) 101
Eco57I CTGAAG 3 cut(s) 150, 579, 764
EcoRII CCWGG 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 537
FaeI CATG 5 cut(s) 155, 193, 277, 322, 408
FalI AAGNNNNNCTT 2 cut(s) 748, 780
FaqI GGGAC 1 cut(s) 655
FatI CATG 5 cut(s) 151, 189, 273, 318, 404
FauNDI CATATG 1 cut(s) 329
FblI GTMKAC 1 cut(s) 13
Fnu4HI GCNGC 4 cut(s) 341, 376, 429, 554
FokI GGATG 7 cut(s) 404, 446, 490, 602, 789, 794, 852
Fsp4HI GCNGC 4 cut(s) 341, 376, 429, 554
FspBI CTAG 2 cut(s) 645, 695
GluI GCNGC 4 cut(s) 341, 376, 429, 554
HaeIII GGCC 1 cut(s) 718
HapII CCGG 3 cut(s) 67, 167, 568
Hin1II CATG 5 cut(s) 155, 193, 277, 322, 408
HincII GTYRAC 1 cut(s) 14
HindII GTYRAC 1 cut(s) 14
HinfI GANTC 2 cut(s) 281, 613
HpaII CCGG 3 cut(s) 67, 167, 568
HphI GGTGA 1 cut(s) 820
Hpy166II GTNNAC 4 cut(s) 14, 123, 565, 762
Hpy188I TCNGA 5 cut(s) 163, 337, 670, 744, 754
Hpy188III TCNNGA 3 cut(s) 152, 548, 695
Hpy8I GTNNAC 4 cut(s) 14, 123, 565, 762
Hpy99I CGWCG 2 cut(s) 15, 84
HpyAV CCTTC 1 cut(s) 589
HpyCH4III ACNGT 3 cut(s) 232, 652, 801
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 6 cut(s) 64, 236, 251, 487, 503, 619
HpyF10VI GCNNNNNNNGC 1 cut(s) 195
HpyF3I CTNAG 3 cut(s) 44, 497, 791
HpySE526I ACGT 1 cut(s) 510
Hsp92II CATG 5 cut(s) 155, 193, 277, 322, 408
Kzo9I GATC 1 cut(s) 736
LmnI GCTCC 3 cut(s) 195, 217, 338
Lsp1109I GCAGC 4 cut(s) 327, 362, 415, 565
LweI GCATC 3 cut(s) 426, 512, 628
MaeI CTAG 2 cut(s) 645, 695
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 1 cut(s) 652
MalI GATC 1 cut(s) 738
MboI GATC 1 cut(s) 736
MboII GAAGA 4 cut(s) 25, 143, 281, 731
MflI RGATCY 1 cut(s) 736
MluCI AATT 8 cut(s) 51, 140, 216, 399, 578, 725, 830, 855
MmeI TCCRAC 1 cut(s) 531
MnlI CCTC 5 cut(s) 108, 296, 524, 631, 727
MroXI GAANNNNTTC 3 cut(s) 32, 54, 400
MseI TTAA 6 cut(s) 138, 219, 297, 543, 828, 837
MslI CAYNNNNRTG 1 cut(s) 498
MspA1I CMGCKG 1 cut(s) 707
MspI CCGG 3 cut(s) 67, 167, 568
MspR9I CCNGG 2 cut(s) 167, 474
MvaI CCWGG 1 cut(s) 474
MwoI GCNNNNNNNGC 1 cut(s) 195
NciI CCSGG 1 cut(s) 167
NdeI CATATG 1 cut(s) 329
NdeII GATC 1 cut(s) 736
NlaIII CATG 5 cut(s) 155, 193, 277, 322, 408
NmeAIII GCCGAG 2 cut(s) 267, 422
NspI RCATGY 1 cut(s) 193
NspV TTCGAA 1 cut(s) 267
PagI TCATGA 1 cut(s) 151
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PdmI GAANNNNTTC 3 cut(s) 32, 54, 400
PfeI GAWTC 2 cut(s) 281, 613
PkrI GCNGC 4 cut(s) 342, 377, 430, 555
Psp6I CCWGG 1 cut(s) 472
PspGI CCWGG 1 cut(s) 472
PspPI GGNCC 1 cut(s) 101
PstNI CAGNNNCTG 1 cut(s) 469
PsuI RGATCY 1 cut(s) 736
PvuII CAGCTG 1 cut(s) 707
RsaI GTAC 4 cut(s) 122, 566, 649, 763
RsaNI GTAC 4 cut(s) 121, 565, 648, 762
RseI CAYNNNNRTG 1 cut(s) 498
SalI GTCGAC 1 cut(s) 12
SaqAI TTAA 6 cut(s) 138, 219, 297, 543, 828, 837
SatI GCNGC 4 cut(s) 341, 376, 429, 554
Sau3AI GATC 1 cut(s) 736
Sau96I GGNCC 1 cut(s) 101
ScaI AGTACT 1 cut(s) 649
ScrFI CCNGG 2 cut(s) 167, 474
SfaNI GCATC 3 cut(s) 426, 512, 628
SfuI TTCGAA 1 cut(s) 267
SgrAI CRCCGGYG 1 cut(s) 66
SinI GGWCC 1 cut(s) 101
SmiMI CAYNNNNRTG 1 cut(s) 498
SmlI CTYRAG 3 cut(s) 112, 183, 548
SmoI CTYRAG 3 cut(s) 112, 183, 548
SpeI ACTAGT 1 cut(s) 644
Sse9I AATT 8 cut(s) 51, 140, 216, 399, 578, 725, 830, 855
SsiI CCGC 4 cut(s) 99, 343, 415, 431
SspI AATATT 2 cut(s) 260, 310
SspMI CTAG 2 cut(s) 645, 695
StyD4I CCNGG 2 cut(s) 165, 472
StyI CCWWGG 1 cut(s) 537
TaaI ACNGT 3 cut(s) 232, 652, 801
TaiI ACGT 1 cut(s) 513
TaqI TCGA 4 cut(s) 13, 267, 381, 519
TasI AATT 8 cut(s) 51, 140, 216, 399, 578, 725, 830, 855
TatI WGTACW 3 cut(s) 120, 647, 761
TfiI GAWTC 2 cut(s) 281, 613
Tru1I TTAA 6 cut(s) 138, 219, 297, 543, 828, 837
Tru9I TTAA 6 cut(s) 138, 219, 297, 543, 828, 837
TscAI CASTG 2 cut(s) 205, 505
TseI GCWGC 4 cut(s) 340, 375, 428, 553
TspDTI ATGAA 4 cut(s) 196, 335, 393, 833
TspRI CASTG 2 cut(s) 205, 505
VpaK11BI GGWCC 1 cut(s) 101
XapI RAATTY 2 cut(s) 51, 399
XbaI TCTAGA 1 cut(s) 694
XceI RCATGY 1 cut(s) 193
XcmI CCANNNNNNNNNTGG 1 cut(s) 470
XmiI GTMKAC 1 cut(s) 13
XmnI GAANNNNTTC 3 cut(s) 32, 54, 400
XspI CTAG 2 cut(s) 645, 695
ZrmI AGTACT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.