RchiOBHm_Chr2g0117071

5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0021364
Physical Location & Seq
Reverse (-)
29375398 .. 29376595
1198 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49006

Sequence Viewer

Length: 477 bp
ATGACTACAACAAGGTATGGAACTCTACGTTCCGAAGCTGAAGCTGATACTAGTGTACACGAGTTCTTCAAAACTTCGTATTTCAAGACTGGAATCCAACCACTCCTGGGTGCTCAGGAGGCACTTCGTAAGTTGTCAGCAAGTTGTAACATGTCAGTTGTAACTTTGCGGCAGAACATTCTTAAGCAGCAGACATTTCAATGGCTTGAAGAGCATTATCCTGGACTGTTTCAAGGGATTCACTTTGGCAACCAGTATGGAGCAAAGGTTTTAATTGACGACAATCCAAAATATGCAATTGACAGTGCCAAGGTTGGGATTAGGGTAGTTCTACTTTTCGATTATGAGAACTCATATACATGGTCTAAGACGGAGTCTATTAATCAACCCCCCTTGATCACCAAGGTTCATAAATGGGAAGCAGTGGAGAAGCAGTTAACGGGATTAATACACAAGACTAGTGATCCTTATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT5C

Protein Analysis

158

Amino Acids

18.11

Weight (kDa)

7.83

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT5C PF06941 13 - 103 1.2e-09 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021364)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0117071
rosa_multiflora Rmu_sc0027521.1_g000002
rosa_roxburghii Rroxscaffold_2G00126740
rosa_samantha Rh2BG277900 Rh2DG292200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 169
AclWI GGATC 1 cut(s) 458
AcuI CTGAAG 1 cut(s) 60
AfaI GTAC 1 cut(s) 57
AfiI CCNNNNNNNGG 2 cut(s) 107, 315
AflII CTTAAG 1 cut(s) 182
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 5 cut(s) 70, 85, 200, 209, 233
AhlI ACTAGT 2 cut(s) 50, 458
AjnI CCWGG 2 cut(s) 105, 220
AluBI AGCT 2 cut(s) 38, 44
AluI AGCT 2 cut(s) 38, 44
Alw21I GWGCWC 1 cut(s) 115
AlwI GGATC 1 cut(s) 458
ApeKI GCWGC 1 cut(s) 187
AseI ATTAAT 2 cut(s) 381, 446
AsuHPI GGTGA 1 cut(s) 391
BaeI ACNNNNGTAYC 2 cut(s) 39, 72
BauI CACGAG 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 115
BbvI GCAGC 1 cut(s) 199
BciT130I CCWGG 2 cut(s) 107, 222
BclI TGATCA 1 cut(s) 396
BcuI ACTAGT 2 cut(s) 50, 458
BfaI CTAG 2 cut(s) 51, 459
BfrI CTTAAG 1 cut(s) 182
BisI GCNGC 2 cut(s) 170, 188
BlsI GCNGC 2 cut(s) 171, 189
Bme1390I CCNGG 2 cut(s) 107, 222
BmrFI CCNGG 2 cut(s) 107, 222
Bpu10I CCTNAGC 1 cut(s) 114
BsaJI CCNNGG 3 cut(s) 106, 309, 402
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 419, 449
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 315
Bse1I ACTGG 2 cut(s) 94, 253
BseBI CCWGG 2 cut(s) 107, 222
BseDI CCNNGG 3 cut(s) 106, 309, 402
BseLI CCNNNNNNNGG 2 cut(s) 107, 315
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 2 cut(s) 94, 253
BseXI GCAGC 1 cut(s) 199
BsiHKAI GWGCWC 1 cut(s) 115
BslI CCNNNNNNNGG 2 cut(s) 107, 315
Bsp1286I GDGCHC 1 cut(s) 115
Bsp1407I TGTACA 1 cut(s) 55
Bsp143I GATC 2 cut(s) 396, 463
BspACI CCGC 1 cut(s) 169
BspCNI CTCAG 1 cut(s) 127
BspPI GGATC 1 cut(s) 458
BspQI GCTCTTC 1 cut(s) 204
BspTI CTTAAG 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 55
BsrI ACTGG 2 cut(s) 94, 253
BssECI CCNNGG 3 cut(s) 106, 309, 402
BssMI GATC 2 cut(s) 396, 463
BssSI CACGAG 1 cut(s) 59
BssT1I CCWWGG 2 cut(s) 309, 402
Bst2BI CACGAG 1 cut(s) 59
Bst2UI CCWGG 2 cut(s) 107, 222
Bst4CI ACNGT 2 cut(s) 228, 305
Bst6I CTCTTC 1 cut(s) 204
BstAFI CTTAAG 1 cut(s) 182
BstAUI TGTACA 1 cut(s) 55
BstDEI CTNAG 2 cut(s) 114, 366
BstKTI GATC 2 cut(s) 399, 466
BstMBI GATC 2 cut(s) 396, 463
BstMWI GCNNNNNNNGC 2 cut(s) 119, 211
BstNI CCWGG 2 cut(s) 107, 222
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 2 cut(s) 105, 220
BstV1I GCAGC 1 cut(s) 199
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 2 cut(s) 310, 429
Csp6I GTAC 1 cut(s) 56
CviAII CATG 2 cut(s) 151, 360
CviJI RGCY 3 cut(s) 38, 44, 205
CviKI_1 RGCY 3 cut(s) 38, 44, 205
CviQI GTAC 1 cut(s) 56
DdeI CTNAG 2 cut(s) 114, 366
DpnI GATC 2 cut(s) 398, 465
DpnII GATC 2 cut(s) 396, 463
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Eco130I CCWWGG 2 cut(s) 309, 402
Eco57I CTGAAG 1 cut(s) 60
EcoRII CCWGG 2 cut(s) 105, 220
EcoT14I CCWWGG 2 cut(s) 309, 402
ErhI CCWWGG 2 cut(s) 309, 402
FaeI CATG 2 cut(s) 154, 363
FaiI YATR 9 cut(s) 18, 152, 258, 294, 345, 355, 357, 361, 411
FatI CATG 2 cut(s) 150, 359
FbaI TGATCA 1 cut(s) 396
Fnu4HI GCNGC 2 cut(s) 170, 188
Fsp4HI GCNGC 2 cut(s) 170, 188
FspBI CTAG 2 cut(s) 51, 459
GluI GCNGC 2 cut(s) 170, 188
Hin1II CATG 2 cut(s) 154, 363
HincII GTYRAC 1 cut(s) 438
HindII GTYRAC 1 cut(s) 438
HinfI GANTC 3 cut(s) 93, 238, 374
HpaI GTTAAC 1 cut(s) 438
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 3 cut(s) 56, 58, 438
Hpy188I TCNGA 1 cut(s) 34
Hpy188III TCNNGA 2 cut(s) 85, 116
Hpy8I GTNNAC 3 cut(s) 56, 58, 438
HpyCH4III ACNGT 2 cut(s) 228, 305
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 1 cut(s) 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 211
HpyF3I CTNAG 2 cut(s) 114, 366
HpySE526I ACGT 1 cut(s) 28
Hsp92II CATG 2 cut(s) 154, 363
Ksp22I TGATCA 1 cut(s) 396
KspAI GTTAAC 1 cut(s) 438
Kzo9I GATC 2 cut(s) 396, 463
LguI GCTCTTC 1 cut(s) 204
LmnI GCTCC 1 cut(s) 260
LpnPI CCDG 7 cut(s) 75, 92, 101, 119, 207, 234, 266
Lsp1109I GCAGC 1 cut(s) 199
MaeI CTAG 2 cut(s) 51, 459
MaeII ACGT 1 cut(s) 28
MaeIII GTNAC 2 cut(s) 146, 160
MalI GATC 2 cut(s) 398, 465
MboI GATC 2 cut(s) 396, 463
MboII GAAGA 2 cut(s) 58, 221
MfeI CAATTG 1 cut(s) 297
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 2 cut(s) 273, 297
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 1 cut(s) 121
MnlI CCTC 1 cut(s) 112
MseI TTAA 5 cut(s) 183, 272, 381, 437, 446
MslI CAYNNNNRTG 2 cut(s) 199, 358
MspCI CTTAAG 1 cut(s) 182
MspR9I CCNGG 2 cut(s) 107, 222
MunI CAATTG 1 cut(s) 297
MvaI CCWGG 2 cut(s) 107, 222
MwoI GCNNNNNNNGC 2 cut(s) 119, 211
NdeII GATC 2 cut(s) 396, 463
NlaIII CATG 2 cut(s) 154, 363
NspI RCATGY 1 cut(s) 154
PciI ACATGT 1 cut(s) 150
PciSI GCTCTTC 1 cut(s) 204
PfeI GAWTC 2 cut(s) 93, 238
PflFI GACNNNGTC 1 cut(s) 373
PfoI TCCNGGA 1 cut(s) 220
PkrI GCNGC 2 cut(s) 171, 189
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PscI ACATGT 1 cut(s) 150
PshBI ATTAAT 2 cut(s) 381, 446
Psp6I CCWGG 2 cut(s) 105, 220
PspGI CCWGG 2 cut(s) 105, 220
PsyI GACNNNGTC 1 cut(s) 373
RsaI GTAC 1 cut(s) 57
RsaNI GTAC 1 cut(s) 56
RseI CAYNNNNRTG 2 cut(s) 199, 358
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 5 cut(s) 183, 272, 381, 437, 446
SatI GCNGC 2 cut(s) 170, 188
Sau3AI GATC 2 cut(s) 396, 463
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 2 cut(s) 107, 222
SduI GDGCHC 1 cut(s) 115
SetI ASST 7 cut(s) 17, 31, 40, 46, 270, 315, 408
SmiMI CAYNNNNRTG 2 cut(s) 199, 358
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SpeI ACTAGT 2 cut(s) 50, 458
Sse9I AATT 2 cut(s) 273, 297
SsiI CCGC 1 cut(s) 169
SspMI CTAG 2 cut(s) 51, 459
StyD4I CCNGG 2 cut(s) 105, 220
StyI CCWWGG 2 cut(s) 309, 402
TaaI ACNGT 2 cut(s) 228, 305
TaiI ACGT 1 cut(s) 31
TaqI TCGA 1 cut(s) 339
TasI AATT 2 cut(s) 273, 297
TatI WGTACW 1 cut(s) 55
TauI GCSGC 1 cut(s) 172
TfiI GAWTC 2 cut(s) 93, 238
Tru1I TTAA 5 cut(s) 183, 272, 381, 437, 446
Tru9I TTAA 5 cut(s) 183, 272, 381, 437, 446
TscAI CASTG 2 cut(s) 310, 429
TseI GCWGC 1 cut(s) 187
TspDTI ATGAA 1 cut(s) 398
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 2 cut(s) 310, 429
Tth111I GACNNNGTC 1 cut(s) 373
Vha464I CTTAAG 1 cut(s) 182
VspI ATTAAT 2 cut(s) 381, 446
XceI RCATGY 1 cut(s) 154
XspI CTAG 2 cut(s) 51, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.