RchiOBHm_Chr3g0474391

1-aminocyclopropane-1-carboxylate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0021380
Physical Location & Seq
Forward (+)
20053530 .. 20054691
1162 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44001

Sequence Viewer

Length: 531 bp
ATGTATGCCAGCAATAACGACTTGTACGGTACAAAGGCGGCGAGCTGGCATGACTCGTTGCAGGTCTGGATGGGCCCACAACGTTCCGAGGCAGAGGAGATCCTAGAGATGTGCAGGAATGAGGTGGTTGCGTGGGATTTTCATGCAACCAAGGTGGGTGAAACTGTGATGGAGTTGCTTTCAGAAGGGTTAGGGTTGGAAGCCGGGAAGTTCAAGGAGCTTACGTTTTCGGATGCGAGGGTGATTGTTGGGCACTGCTATCCCTATTGTCCTCAACCCGATCTCACAAACCATGTACCTGGGTTGGAAGTTAGGCATCAAAATGAGTGGGTGGATGTCAAGCCTGTGCTTGGAGGTTTGGTTATCAACATTGGAGATCTTCTTCAGATAATCTCTAATGGGGAGTATAAAAGTGTGCAACATCGTGTGTTGGCAAACTCCTGGAAGGAAGCTCGAATTTCAATTGTGATATTTTACAACCTAGCCAAATGGAGGGAAGATGGATATTGTGGACCTCTCCCAGAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

176

Amino Acids

19.9

Weight (kDa)

5.09

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 96 - 160 8e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021380)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0474391
rosa_samantha Rh3AG191300 Rh3BG220500 Rh3CG215800 Rh3DG216200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 52
AciI CCGC 1 cut(s) 38
AclI AACGTT 1 cut(s) 82
AclWI GGATC 1 cut(s) 94
AcsI RAATTY 1 cut(s) 456
AcuI CTGAAG 1 cut(s) 368
AfaI GTAC 3 cut(s) 26, 31, 297
AfiI CCNNNNNNNGG 2 cut(s) 350, 492
AgsI TTSAA 2 cut(s) 214, 462
AjnI CCWGG 2 cut(s) 298, 440
AluBI AGCT 3 cut(s) 45, 220, 452
AluI AGCT 3 cut(s) 45, 220, 452
AlwI GGATC 1 cut(s) 94
AoxI GGCC 1 cut(s) 73
ApaI GGGCCC 1 cut(s) 77
ApoI RAATTY 1 cut(s) 456
AspS9I GGNCC 3 cut(s) 73, 74, 512
AsuC2I CCSGG 1 cut(s) 205
AsuHPI GGTGA 2 cut(s) 170, 253
AvaII GGWCC 1 cut(s) 512
BaeGI GKGCMC 2 cut(s) 77, 255
BanII GRGCYC 1 cut(s) 77
BccI CCATC 3 cut(s) 64, 163, 494
BciT130I CCWGG 2 cut(s) 300, 442
BcnI CCSGG 1 cut(s) 205
BfaI CTAG 2 cut(s) 104, 482
BfuAI ACCTGC 1 cut(s) 52
BglII AGATCT 1 cut(s) 376
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme1390I CCNGG 3 cut(s) 205, 300, 442
Bme18I GGWCC 1 cut(s) 512
BmgT120I GGNCC 3 cut(s) 73, 74, 512
BmiI GGNNCC 1 cut(s) 75
BmrFI CCNGG 3 cut(s) 205, 300, 442
BmsI GCATC 2 cut(s) 223, 325
BpuMI CCSGG 1 cut(s) 205
BsaJI CCNNGG 3 cut(s) 87, 150, 299
BsaXI ACNNNNNCTCC 4 cut(s) 209, 239, 345, 375
Bsc4I CCNNNNNNNGG 2 cut(s) 350, 492
BseBI CCWGG 2 cut(s) 300, 442
BseDI CCNNGG 3 cut(s) 87, 150, 299
BseGI GGATG 3 cut(s) 75, 238, 340
BseLI CCNNNNNNNGG 2 cut(s) 350, 492
BseRI GAGGAG 1 cut(s) 110
BseSI GKGCMC 2 cut(s) 77, 255
BsgI GTGCAG 1 cut(s) 133
BshFI GGCC 1 cut(s) 75
BsiSI CCGG 1 cut(s) 204
BslI CCNNNNNNNGG 2 cut(s) 350, 492
BsnI GGCC 1 cut(s) 75
Bsp120I GGGCCC 1 cut(s) 73
Bsp1286I GDGCHC 2 cut(s) 77, 255
Bsp143I GATC 3 cut(s) 99, 280, 376
BspACI CCGC 1 cut(s) 38
BspANI GGCC 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 75
BspMI ACCTGC 1 cut(s) 52
BspPI GGATC 1 cut(s) 94
BssECI CCNNGG 3 cut(s) 87, 150, 299
BssMI GATC 3 cut(s) 99, 280, 376
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 2 cut(s) 300, 442
Bst4CI ACNGT 2 cut(s) 29, 166
BstC8I GCNNGC 3 cut(s) 10, 43, 47
BstF5I GGATG 3 cut(s) 75, 238, 340
BstKTI GATC 3 cut(s) 102, 283, 379
BstMBI GATC 3 cut(s) 99, 280, 376
BstNI CCWGG 2 cut(s) 300, 442
BstSCI CCNGG 3 cut(s) 203, 298, 440
BstSLI GKGCMC 2 cut(s) 77, 255
BstX2I RGATCY 2 cut(s) 99, 376
BstXI CCANNNNNNTGG 1 cut(s) 299
BstYI RGATCY 2 cut(s) 99, 376
BsuRI GGCC 1 cut(s) 75
BtsCI GGATG 3 cut(s) 75, 238, 340
BtsI GCAGTG 1 cut(s) 253
BtsIMutI CAGTG 1 cut(s) 253
BveI ACCTGC 1 cut(s) 52
Cac8I GCNNGC 3 cut(s) 10, 43, 47
Cfr13I GGNCC 3 cut(s) 73, 74, 512
Csp6I GTAC 3 cut(s) 25, 30, 296
CspCI CAANNNNNGTGG 4 cut(s) 135, 170, 308, 343
CviAII CATG 3 cut(s) 50, 143, 293
CviJI RGCY 7 cut(s) 45, 75, 203, 220, 343, 452, 485
CviKI_1 RGCY 7 cut(s) 45, 75, 203, 220, 343, 452, 485
CviQI GTAC 3 cut(s) 25, 30, 296
DpnI GATC 3 cut(s) 101, 282, 378
DpnII GATC 3 cut(s) 99, 280, 376
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 77
Eco47I GGWCC 1 cut(s) 512
Eco57I CTGAAG 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 298, 440
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 77
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 3 cut(s) 53, 146, 296
FaiI YATR 5 cut(s) 6, 51, 144, 294, 408
FatI CATG 3 cut(s) 49, 142, 292
Fnu4HI GCNGC 1 cut(s) 39
FokI GGATG 3 cut(s) 82, 245, 347
FriOI GRGCYC 1 cut(s) 77
Fsp4HI GCNGC 1 cut(s) 39
FspBI CTAG 2 cut(s) 104, 482
GluI GCNGC 1 cut(s) 39
HaeIII GGCC 1 cut(s) 75
HapII CCGG 1 cut(s) 204
Hin1II CATG 3 cut(s) 53, 146, 296
HinfI GANTC 1 cut(s) 53
HpaII CCGG 1 cut(s) 204
HphI GGTGA 2 cut(s) 170, 253
Hpy166II GTNNAC 1 cut(s) 512
Hpy188I TCNGA 4 cut(s) 88, 184, 232, 387
Hpy188III TCNNGA 1 cut(s) 67
Hpy8I GTNNAC 1 cut(s) 512
HpyAV CCTTC 2 cut(s) 179, 439
HpyCH4III ACNGT 2 cut(s) 29, 166
HpyCH4IV ACGT 2 cut(s) 82, 224
HpyCH4V TGCA 4 cut(s) 61, 114, 146, 418
HpySE526I ACGT 2 cut(s) 82, 224
Hsp92II CATG 3 cut(s) 53, 146, 296
Kzo9I GATC 3 cut(s) 99, 280, 376
LmnI GCTCC 1 cut(s) 217
LweI GCATC 2 cut(s) 223, 325
MaeI CTAG 2 cut(s) 104, 482
MaeII ACGT 2 cut(s) 82, 224
MalI GATC 3 cut(s) 101, 282, 378
MboI GATC 3 cut(s) 99, 280, 376
MboII GAAGA 3 cut(s) 371, 374, 509
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 2 cut(s) 99, 376
MhlI GDGCHC 2 cut(s) 77, 255
MluCI AATT 2 cut(s) 456, 462
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 2 cut(s) 177, 285
MnlI CCTC 8 cut(s) 82, 88, 115, 231, 282, 347, 486, 525
MslI CAYNNNNRTG 1 cut(s) 321
MspI CCGG 1 cut(s) 204
MspR9I CCNGG 3 cut(s) 205, 300, 442
MunI CAATTG 1 cut(s) 462
MvaI CCWGG 2 cut(s) 300, 442
NciI CCSGG 1 cut(s) 205
NdeII GATC 3 cut(s) 99, 280, 376
NlaIII CATG 3 cut(s) 53, 146, 296
NlaIV GGNNCC 1 cut(s) 75
PfoI TCCNGGA 1 cut(s) 440
PkrI GCNGC 1 cut(s) 40
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
Psp1406I AACGTT 1 cut(s) 82
Psp6I CCWGG 2 cut(s) 298, 440
PspGI CCWGG 2 cut(s) 298, 440
PspN4I GGNNCC 1 cut(s) 75
PspOMI GGGCCC 1 cut(s) 73
PspPI GGNCC 3 cut(s) 73, 74, 512
PsuI RGATCY 2 cut(s) 99, 376
RsaI GTAC 3 cut(s) 26, 31, 297
RsaNI GTAC 3 cut(s) 25, 30, 296
RseI CAYNNNNRTG 1 cut(s) 321
SatI GCNGC 1 cut(s) 39
Sau3AI GATC 3 cut(s) 99, 280, 376
Sau96I GGNCC 3 cut(s) 73, 74, 512
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 3 cut(s) 205, 300, 442
SduI GDGCHC 2 cut(s) 77, 255
SfaNI GCATC 2 cut(s) 223, 325
SinI GGWCC 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 321
Sse9I AATT 2 cut(s) 456, 462
SsiI CCGC 1 cut(s) 38
SspMI CTAG 2 cut(s) 104, 482
StyD4I CCNGG 3 cut(s) 203, 298, 440
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 29, 166
TaiI ACGT 2 cut(s) 85, 227
TaqI TCGA 1 cut(s) 454
TasI AATT 2 cut(s) 456, 462
TauI GCSGC 1 cut(s) 41
TscAI CASTG 1 cut(s) 260
TspDTI ATGAA 1 cut(s) 131
TspRI CASTG 1 cut(s) 260
VpaK11BI GGWCC 1 cut(s) 512
XapI RAATTY 1 cut(s) 456
XspI CTAG 2 cut(s) 104, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.