Rh3AG191300

1-aminocyclopropane-1-carboxylate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0021380
Physical Location & Seq
Forward (+)
18298973 .. 18315871
16899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG191300.1

Sequence Viewer

Length: 678 bp
ATGATCGGACTAAATGACCTAGGGGGTGGCCTGATCAATCTCGCCTCGAAAACATGGGGTTTCTTCCGAGTAATCAACCACAACGTACCCGTTTCGATACTCGATGAGACGTTGAAGGCTGTTAAAGCGTTTCACGACCAGCCCCACGAGGTGAAGGCGAAGTACTACAAGCGAGAAGAGGGAAAGGGTGTCATGTATGCCAGCAATAACGACTTGTACGGTACAAAGGCGGCGAGCTGGCATGACTCGTTGCAGGTCTGGATGGGCCCACAACGTTCCGAGGCAGAGGAGATCCTAGAGATGTGCAGGAATGAGGTGGTTGCGTGGGATTTTCATGCAACCAAGGTGGGTGAAACTGTGATGGAGTTGCTTTCAGAAGGGTTAGGGTTGGAAGCCGGGAAGTTCAAGGAGCTTACGTTTTCGGATGCGAGGAACCATGTACCTGGGTTGGAAGTTAGGCATCAAAATGAGTGGGTGGATGTCAAGCCTGTGCTTGGAGGTTTGGTTATCAACATTGGAGATCTTCTTCAGATAATCTCTAATGGGGAGTATAAAAGTGTGCAACATCGTGTGTTGGCAAACTCCTGGAAGGAAGCTCGAATTTCAATTGCAACTTCTTCAACCTTTGTAAATGCCTTCAAATTACTTTGGAAGGCTATTCTGGACACCACAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

225

Amino Acids

25.21

Weight (kDa)

6.07

Isoelectric Point (pI)

33.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 12 - 89 8e-13 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 145 - 205 5.9e-15 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021380)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0474391
rosa_samantha Rh3AG191300 Rh3BG220500 Rh3CG215800 Rh3DG216200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 244
AciI CCGC 1 cut(s) 230
AclI AACGTT 1 cut(s) 274
AclWI GGATC 1 cut(s) 286
AcsI RAATTY 1 cut(s) 600
AcuI CTGAAG 1 cut(s) 512
AdeI CACNNNGTG 1 cut(s) 151
AfaI GTAC 5 cut(s) 87, 164, 218, 223, 441
AfiI CCNNNNNNNGG 1 cut(s) 494
AgsI TTSAA 5 cut(s) 115, 406, 606, 621, 640
AjnI CCWGG 2 cut(s) 442, 584
AluBI AGCT 3 cut(s) 237, 412, 596
AluI AGCT 3 cut(s) 237, 412, 596
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 1 cut(s) 286
AoxI GGCC 2 cut(s) 28, 265
ApaI GGGCCC 1 cut(s) 269
ApoI RAATTY 1 cut(s) 600
AspA2I CCTAGG 1 cut(s) 19
AspS9I GGNCC 2 cut(s) 265, 266
AsuC2I CCSGG 1 cut(s) 397
AsuHPI GGTGA 2 cut(s) 163, 362
AvrII CCTAGG 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 269
BarI GAAGNNNNNNTAC 2 cut(s) 146, 178
BauI CACGAG 1 cut(s) 146
BccI CCATC 2 cut(s) 256, 355
BciT130I CCWGG 2 cut(s) 444, 586
BclI TGATCA 1 cut(s) 33
BcnI CCSGG 1 cut(s) 397
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 2 cut(s) 20, 296
BfuAI ACCTGC 1 cut(s) 244
BglII AGATCT 1 cut(s) 520
BisI GCNGC 1 cut(s) 231
BlnI CCTAGG 1 cut(s) 19
BlsI GCNGC 1 cut(s) 232
BmcAI AGTACT 1 cut(s) 164
Bme1390I CCNGG 3 cut(s) 397, 444, 586
BmgT120I GGNCC 2 cut(s) 265, 266
BmiI GGNNCC 2 cut(s) 267, 434
BmrFI CCNGG 3 cut(s) 397, 444, 586
BmsI GCATC 2 cut(s) 415, 469
BpuMI CCSGG 1 cut(s) 397
BsaJI CCNNGG 4 cut(s) 19, 279, 342, 443
BsaXI ACNNNNNCTCC 4 cut(s) 401, 431, 489, 519
Bsc4I CCNNNNNNNGG 1 cut(s) 494
BseBI CCWGG 2 cut(s) 444, 586
BseDI CCNNGG 4 cut(s) 19, 279, 342, 443
BseGI GGATG 3 cut(s) 267, 430, 484
BseLI CCNNNNNNNGG 1 cut(s) 494
BseRI GAGGAG 1 cut(s) 302
BseSI GKGCMC 1 cut(s) 269
BsgI GTGCAG 1 cut(s) 325
BshFI GGCC 2 cut(s) 30, 267
BsiSI CCGG 1 cut(s) 396
BslI CCNNNNNNNGG 1 cut(s) 494
BsmAI GTCTC 1 cut(s) 101
BsmBI CGTCTC 1 cut(s) 101
BsnI GGCC 2 cut(s) 30, 267
Bsp120I GGGCCC 1 cut(s) 265
Bsp1286I GDGCHC 1 cut(s) 269
Bsp143I GATC 4 cut(s) 3, 33, 291, 520
BspACI CCGC 1 cut(s) 230
BspANI GGCC 2 cut(s) 30, 267
BspLI GGNNCC 2 cut(s) 267, 434
BspMI ACCTGC 1 cut(s) 244
BspPI GGATC 1 cut(s) 286
BssECI CCNNGG 4 cut(s) 19, 279, 342, 443
BssMI GATC 4 cut(s) 3, 33, 291, 520
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 2 cut(s) 19, 342
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 2 cut(s) 444, 586
Bst4CI ACNGT 2 cut(s) 221, 358
Bst6I CTCTTC 1 cut(s) 171
BstC8I GCNNGC 3 cut(s) 202, 235, 239
BstF5I GGATG 3 cut(s) 267, 430, 484
BstKTI GATC 4 cut(s) 6, 36, 294, 523
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 4 cut(s) 3, 33, 291, 520
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNI CCWGG 2 cut(s) 444, 586
BstSCI CCNGG 3 cut(s) 395, 442, 584
BstSLI GKGCMC 1 cut(s) 269
BstX2I RGATCY 2 cut(s) 291, 520
BstXI CCANNNNNNTGG 1 cut(s) 443
BstYI RGATCY 2 cut(s) 291, 520
BsuRI GGCC 2 cut(s) 30, 267
BtsCI GGATG 3 cut(s) 267, 430, 484
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 3 cut(s) 202, 235, 239
Cfr13I GGNCC 2 cut(s) 265, 266
Csp6I GTAC 5 cut(s) 86, 163, 217, 222, 440
CspCI CAANNNNNGTGG 4 cut(s) 327, 362, 452, 487
CviAII CATG 5 cut(s) 54, 193, 242, 335, 437
CviQI GTAC 5 cut(s) 86, 163, 217, 222, 440
DpnI GATC 4 cut(s) 5, 35, 293, 522
DpnII GATC 4 cut(s) 3, 33, 291, 520
DraIII CACNNNGTG 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 19, 342
Eco24I GRGCYC 1 cut(s) 269
Eco57I CTGAAG 1 cut(s) 512
EcoRII CCWGG 2 cut(s) 442, 584
EcoT14I CCWWGG 2 cut(s) 19, 342
EcoT38I GRGCYC 1 cut(s) 269
ErhI CCWWGG 2 cut(s) 19, 342
Esp3I CGTCTC 1 cut(s) 101
FaeI CATG 5 cut(s) 57, 196, 245, 338, 440
FaiI YATR 7 cut(s) 55, 194, 198, 243, 336, 438, 552
FatI CATG 5 cut(s) 53, 192, 241, 334, 436
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 3 cut(s) 274, 437, 491
FriOI GRGCYC 1 cut(s) 269
Fsp4HI GCNGC 1 cut(s) 231
FspBI CTAG 2 cut(s) 20, 296
GluI GCNGC 1 cut(s) 231
HaeIII GGCC 2 cut(s) 30, 267
HapII CCGG 1 cut(s) 396
Hin1II CATG 5 cut(s) 57, 196, 245, 338, 440
HinfI GANTC 1 cut(s) 245
HpaII CCGG 1 cut(s) 396
HphI GGTGA 2 cut(s) 163, 362
Hpy188I TCNGA 6 cut(s) 8, 68, 280, 376, 424, 531
Hpy188III TCNNGA 3 cut(s) 134, 259, 662
HpyAV CCTTC 6 cut(s) 109, 148, 371, 583, 646, 646
HpyCH4III ACNGT 2 cut(s) 221, 358
HpyCH4IV ACGT 4 cut(s) 84, 110, 274, 416
HpyCH4V TGCA 5 cut(s) 253, 306, 338, 562, 611
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpySE526I ACGT 4 cut(s) 84, 110, 274, 416
Hsp92II CATG 5 cut(s) 57, 196, 245, 338, 440
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 4 cut(s) 3, 33, 291, 520
LmnI GCTCC 1 cut(s) 409
LweI GCATC 2 cut(s) 415, 469
MaeI CTAG 2 cut(s) 20, 296
MaeII ACGT 4 cut(s) 84, 110, 274, 416
MalI GATC 4 cut(s) 5, 35, 293, 522
MboI GATC 4 cut(s) 3, 33, 291, 520
MboII GAAGA 5 cut(s) 55, 188, 515, 518, 609
MfeI CAATTG 1 cut(s) 606
MflI RGATCY 2 cut(s) 291, 520
MhlI GDGCHC 1 cut(s) 269
MluCI AATT 4 cut(s) 600, 606, 641, 672
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 2 cut(s) 369, 429
MnlI CCTC 8 cut(s) 55, 142, 172, 274, 280, 307, 423, 491
MseI TTAA 1 cut(s) 123
MslI CAYNNNNRTG 1 cut(s) 465
MspI CCGG 1 cut(s) 396
MspR9I CCNGG 3 cut(s) 397, 444, 586
MunI CAATTG 1 cut(s) 606
MvaI CCWGG 2 cut(s) 444, 586
MwoI GCNNNNNNNGC 1 cut(s) 125
NciI CCSGG 1 cut(s) 397
NdeII GATC 4 cut(s) 3, 33, 291, 520
NlaIII CATG 5 cut(s) 57, 196, 245, 338, 440
NlaIV GGNNCC 2 cut(s) 267, 434
PfoI TCCNGGA 1 cut(s) 584
PkrI GCNGC 1 cut(s) 232
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
Psp1406I AACGTT 1 cut(s) 274
Psp6I CCWGG 2 cut(s) 442, 584
PspGI CCWGG 2 cut(s) 442, 584
PspN4I GGNNCC 2 cut(s) 267, 434
PspOMI GGGCCC 1 cut(s) 265
PspPI GGNCC 2 cut(s) 265, 266
PsuI RGATCY 2 cut(s) 291, 520
RsaI GTAC 5 cut(s) 87, 164, 218, 223, 441
RsaNI GTAC 5 cut(s) 86, 163, 217, 222, 440
RseI CAYNNNNRTG 1 cut(s) 465
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 1 cut(s) 231
Sau3AI GATC 4 cut(s) 3, 33, 291, 520
Sau96I GGNCC 2 cut(s) 265, 266
ScaI AGTACT 1 cut(s) 164
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 3 cut(s) 397, 444, 586
SduI GDGCHC 1 cut(s) 269
SfaNI GCATC 2 cut(s) 415, 469
SmiMI CAYNNNNRTG 1 cut(s) 465
Sse9I AATT 4 cut(s) 600, 606, 641, 672
SsiI CCGC 1 cut(s) 230
SspMI CTAG 2 cut(s) 20, 296
StyD4I CCNGG 3 cut(s) 395, 442, 584
StyI CCWWGG 2 cut(s) 19, 342
TaaI ACNGT 2 cut(s) 221, 358
TaiI ACGT 4 cut(s) 87, 113, 277, 419
TaqI TCGA 4 cut(s) 47, 95, 102, 598
TasI AATT 4 cut(s) 600, 606, 641, 672
TatI WGTACW 1 cut(s) 162
TauI GCSGC 1 cut(s) 233
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TspDTI ATGAA 1 cut(s) 323
XapI RAATTY 1 cut(s) 600
XmaJI CCTAGG 1 cut(s) 19
XspI CTAG 2 cut(s) 20, 296
ZrmI AGTACT 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.