RchiOBHm_Chr4g0411741

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0022728
Physical Location & Seq
Forward (+)
35610342 .. 35613002
2661 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38250

Sequence Viewer

Length: 1266 bp
ATGGTGATCATTCACTATGGCTCTTCTTCTTCTTCTTCTTCGTTTTCCAGCCATCCATGGAAATACCATGTCTTTTTGAGTTTTAGAGGTGAGGATACCCGCAATAATTTCACAGGCCATTTGTGCAGCGCTTTGCGTCAGAATAGAATCACCACCTTCATGGATGATCAGCTCAGAAGAGGAGAAGAAATATCAACAGCACTTCTCCAAGCAATTGAACAGTCGAGGATTTCAATCATGGTGTTCTCTAGAAACTATGCATCCTCCAAGTGGTGCTTGGATGAACTTGTCAAGATCCTCGAGTGCAAGAATTCAAATCAACGATTGGTTCGACCAGTTTTCTACAAAGTTGATCCCTCGGATATACGAAATCACAGAGGTAGCTTTGGTAAGGCACTTGCTAATCATGAATGCAGATTCAAGGTTCAGAAATGGAAGGATGCTCTCTCAGAAGCAGCACATTTGTTAGGGTGGCATCTTTTGGATGAGCATTGCTCTAAATCCAGTGTTATTCATGAAATTGTTAAAGAGATTTCAGAGCAAGTTATAAACAGTACATTTATGGATGTGGCCAAGTACCCAGTAGGAATAAAACCTCGGGTGCACAAGATCCATAAGCTTTTAGGTGTTGAGGGAAGAGACGTTCATATGGTAGGGATATGGGGGGTTGGTGGAATAGGTAAGACAACAATTGCCAGAGCTGTCTATAACTCAATTGCTCATAAATTTGATGGGAGTTGTTTTTTGGAAAAAGTTAGAGAAAATTCAATGGGTGCTAAAGGCTTTGTCAAACTACAAAAGAAACTTCTTTGTGAGATTCTAAAGGGAACAAATTTGAAGGTGGCCAATGAAGCTAGAGGAATCACTATGATCAAGGAAATGTTGCAATACAAAAGTGTTCTCTTAGTGCTTGATGATGTGAATGATATTGATCAGTTAAACAATTTAGCCGGTGACTGTAGTTGGTTTGTTAAGGGTAGTAAAATCATCATAACAACAAGGGATAGGCAATTGTTGACCAGTCACGGTGTTAATTTAATATACGAGGTCCAAAAATTTGATCATCATGAAGCTCTCGAGCTTTTTAGTTGGAATGCCTTCAAAAGAAACGGGCCTTTGGATGGTTTTGCGGAACTCATAGAACGTGCAATACACTATGCTCAAGGCCTTCCATTAGTTTTGTGTGTTCTAGGTTCTTATCTATGTGGTGGAAATGTAGACAAATGGCAAGCTGAAGCACAACAATTAGAAAAGTTCTCCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

421

Amino Acids

47.71

Weight (kDa)

8.94

Isoelectric Point (pI)

34.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 184 1.3e-52 TIR domain
TIR_2 PF13676 24 - 119 4.7e-16 TIR domain
NB-ARC PF00931 201 - 369 2.1e-21 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022728)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0411741
rosa_multiflora Rmu_co8266463.1_g000001 Rmu_sc0000711.1_g000029
rosa_roxburghii Rroxscaffold_5G00358000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 548
AccI GTMKAC 1 cut(s) 1218
AciI CCGC 2 cut(s) 100, 1130
AclWI GGATC 3 cut(s) 289, 347, 604
AcoI YGGCCR 2 cut(s) 570, 843
AcsI RAATTY 5 cut(s) 310, 725, 763, 832, 1055
AcuI CTGAAG 1 cut(s) 1254
AfaI GTAC 2 cut(s) 556, 578
AfeI AGCGCT 1 cut(s) 130
AfiI CCNNNNNNNGG 2 cut(s) 270, 1121
AgsI TTSAA 7 cut(s) 218, 234, 315, 421, 768, 838, 1102
AloI GAACNNNNNNTCC 2 cut(s) 627, 659
AluBI AGCT 8 cut(s) 172, 384, 619, 701, 854, 1073, 1081, 1232
AluI AGCT 8 cut(s) 172, 384, 619, 701, 854, 1073, 1081, 1232
Alw21I GWGCWC 1 cut(s) 606
Alw26I GTCTC 1 cut(s) 633
Alw44I GTGCAC 1 cut(s) 602
AlwI GGATC 3 cut(s) 289, 347, 604
Ama87I CYCGRG 3 cut(s) 299, 597, 1076
Aor51HI AGCGCT 1 cut(s) 130
AoxI GGCC 5 cut(s) 115, 570, 843, 1112, 1165
ApaLI GTGCAC 1 cut(s) 602
ApeKI GCWGC 2 cut(s) 126, 455
ApoI RAATTY 5 cut(s) 310, 725, 763, 832, 1055
Asp700I GAANNNNTTC 1 cut(s) 1097
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 1048, 1112
AsuHPI GGTGA 4 cut(s) 16, 101, 142, 965
AvaI CYCGRG 3 cut(s) 299, 597, 1076
AvaII GGWCC 1 cut(s) 1048
BaeGI GKGCMC 1 cut(s) 606
BalI TGGCCA 2 cut(s) 572, 845
Bbv12I GWGCWC 1 cut(s) 606
BbvI GCAGC 2 cut(s) 138, 467
BccI CCATC 3 cut(s) 60, 725, 1115
BciVI GTATCC 1 cut(s) 88
BclI TGATCA 5 cut(s) 6, 166, 870, 931, 1060
BcoDI GTCTC 1 cut(s) 633
BfaI CTAG 3 cut(s) 249, 855, 1190
BfmI CTRYAG 1 cut(s) 958
BfoI RGCGCY 1 cut(s) 132
BfuI GTATCC 1 cut(s) 88
BisI GCNGC 2 cut(s) 127, 456
BlsI GCNGC 2 cut(s) 128, 457
Bme18I GGWCC 1 cut(s) 1048
BmeT110I CYCGRG 3 cut(s) 299, 597, 1076
BmgT120I GGNCC 2 cut(s) 1048, 1112
BmrI ACTGGG 1 cut(s) 575
BmsI GCATC 3 cut(s) 269, 430, 484
BmuI ACTGGG 1 cut(s) 575
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpuEI CTTGAG 1 cut(s) 1146
BsaBI GATNNNNATC 2 cut(s) 233, 930
BsaJI CCNNGG 3 cut(s) 56, 357, 596
Bsc4I CCNNNNNNNGG 2 cut(s) 270, 1121
Bse118I RCCGGY 1 cut(s) 950
Bse1I ACTGG 4 cut(s) 335, 504, 581, 1020
Bse3DI GCAATG 1 cut(s) 490
Bse8I GATNNNNATC 2 cut(s) 233, 930
BseDI CCNNGG 3 cut(s) 56, 357, 596
BseGI GGATG 8 cut(s) 52, 169, 260, 286, 445, 490, 571, 1126
BseJI GATNNNNATC 2 cut(s) 233, 930
BseLI CCNNNNNNNGG 2 cut(s) 270, 1121
BseMI GCAATG 1 cut(s) 490
BseMII CTCAG 2 cut(s) 187, 462
BseNI ACTGG 4 cut(s) 335, 504, 581, 1020
BseRI GAGGAG 1 cut(s) 195
BseSI GKGCMC 1 cut(s) 606
BseXI GCAGC 2 cut(s) 138, 467
BsgI GTGCAG 1 cut(s) 145
BshFI GGCC 5 cut(s) 117, 572, 845, 1114, 1167
BsiHKAI GWGCWC 1 cut(s) 606
BsiHKCI CYCGRG 3 cut(s) 299, 597, 1076
BsiSI CCGG 1 cut(s) 951
BslI CCNNNNNNNGG 2 cut(s) 270, 1121
BsmAI GTCTC 1 cut(s) 633
BsmBI CGTCTC 1 cut(s) 633
BsmI GAATGC 2 cut(s) 416, 1099
BsnI GGCC 5 cut(s) 117, 572, 845, 1114, 1167
BsoBI CYCGRG 3 cut(s) 299, 597, 1076
Bsp1286I GDGCHC 1 cut(s) 606
Bsp143I GATC 8 cut(s) 6, 166, 294, 352, 609, 870, 931, 1060
Bsp19I CCATGG 1 cut(s) 56
BspACI CCGC 2 cut(s) 100, 1130
BspANI GGCC 5 cut(s) 117, 572, 845, 1114, 1167
BspCNI CTCAG 2 cut(s) 186, 461
BspHI TCATGA 3 cut(s) 406, 514, 1066
BspPI GGATC 3 cut(s) 289, 347, 604
BspQI GCTCTTC 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 490
BsrFI RCCGGY 1 cut(s) 950
BsrI ACTGG 4 cut(s) 335, 504, 581, 1020
BssAI RCCGGY 1 cut(s) 950
BssECI CCNNGG 3 cut(s) 56, 357, 596
BssMI GATC 8 cut(s) 6, 166, 294, 352, 609, 870, 931, 1060
BssT1I CCWWGG 1 cut(s) 56
Bst4CI ACNGT 4 cut(s) 222, 554, 959, 1028
Bst6I CTCTTC 3 cut(s) 28, 172, 631
BstC8I GCNNGC 1 cut(s) 1230
BstDEI CTNAG 3 cut(s) 173, 448, 904
BstDSI CCRYGG 1 cut(s) 56
BstF5I GGATG 8 cut(s) 52, 169, 260, 286, 445, 490, 571, 1126
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 8 cut(s) 9, 169, 297, 355, 612, 873, 934, 1063
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 8 cut(s) 6, 166, 294, 352, 609, 870, 931, 1060
BstMWI GCNNNNNNNGC 2 cut(s) 123, 851
BstSFI CTRYAG 1 cut(s) 958
BstSLI GKGCMC 1 cut(s) 606
BstV1I GCAGC 2 cut(s) 138, 467
BstX2I RGATCY 2 cut(s) 294, 609
BstXI CCANNNNNNTGG 1 cut(s) 160
BstYI RGATCY 2 cut(s) 294, 609
BsuI GTATCC 1 cut(s) 88
BsuRI GGCC 5 cut(s) 117, 572, 845, 1114, 1167
BtgI CCRYGG 1 cut(s) 56
BtsCI GGATG 8 cut(s) 52, 169, 260, 286, 445, 490, 571, 1126
BtsIMutI CAGTG 1 cut(s) 511
Cac8I GCNNGC 1 cut(s) 1230
CciI TCATGA 3 cut(s) 406, 514, 1066
CfoI GCGC 1 cut(s) 131
Cfr10I RCCGGY 1 cut(s) 950
Cfr13I GGNCC 2 cut(s) 1048, 1112
CseI GACGC 1 cut(s) 125
Csp6I GTAC 2 cut(s) 555, 577
CviAII CATG 7 cut(s) 57, 68, 160, 238, 407, 515, 1067
CviQI GTAC 2 cut(s) 555, 577
DdeI CTNAG 3 cut(s) 173, 448, 904
DpnI GATC 8 cut(s) 8, 168, 296, 354, 611, 872, 933, 1062
DpnII GATC 8 cut(s) 6, 166, 294, 352, 609, 870, 931, 1060
EaeI YGGCCR 2 cut(s) 570, 843
Eam1104I CTCTTC 3 cut(s) 28, 172, 631
EarI CTCTTC 3 cut(s) 28, 172, 631
Eco130I CCWWGG 1 cut(s) 56
Eco147I AGGCCT 1 cut(s) 1167
Eco47I GGWCC 1 cut(s) 1048
Eco47III AGCGCT 1 cut(s) 130
Eco57I CTGAAG 1 cut(s) 1254
Eco88I CYCGRG 3 cut(s) 299, 597, 1076
EcoRI GAATTC 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 56
EcoT22I ATGCAT 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 56
Esp3I CGTCTC 1 cut(s) 633
FaeI CATG 7 cut(s) 60, 71, 163, 241, 410, 518, 1070
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FatI CATG 7 cut(s) 56, 67, 159, 237, 406, 514, 1066
FauI CCCGC 1 cut(s) 107
FauNDI CATATG 1 cut(s) 648
FbaI TGATCA 5 cut(s) 6, 166, 870, 931, 1060
FblI GTMKAC 1 cut(s) 1218
Fnu4HI GCNGC 2 cut(s) 127, 456
FokI GGATG 8 cut(s) 39, 176, 247, 293, 452, 497, 578, 1133
Fsp4HI GCNGC 2 cut(s) 127, 456
FspBI CTAG 3 cut(s) 249, 855, 1190
GlaI GCGC 1 cut(s) 130
GluI GCNGC 2 cut(s) 127, 456
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 5 cut(s) 117, 572, 845, 1114, 1167
HapII CCGG 1 cut(s) 951
HgaI GACGC 1 cut(s) 125
HhaI GCGC 1 cut(s) 131
Hin1II CATG 7 cut(s) 60, 71, 163, 241, 410, 518, 1070
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 1017
HindII GTYRAC 1 cut(s) 1017
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 4 cut(s) 147, 417, 817, 861
HpaII CCGG 1 cut(s) 951
HphI GGTGA 4 cut(s) 16, 101, 142, 965
Hpy166II GTNNAC 3 cut(s) 604, 1017, 1219
Hpy188I TCNGA 6 cut(s) 141, 176, 361, 429, 451, 538
Hpy188III TCNNGA 6 cut(s) 249, 292, 407, 515, 1067, 1076
Hpy8I GTNNAC 3 cut(s) 604, 1017, 1219
HpyAV CCTTC 5 cut(s) 166, 430, 832, 1108, 1178
HpyCH4III ACNGT 4 cut(s) 222, 554, 959, 1028
HpyCH4IV ACGT 2 cut(s) 642, 1144
HpyCH4V TGCA 7 cut(s) 126, 260, 306, 414, 604, 886, 1148
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 851
HpyF3I CTNAG 3 cut(s) 173, 448, 904
HpySE526I ACGT 2 cut(s) 642, 1144
Hsp92II CATG 7 cut(s) 60, 71, 163, 241, 410, 518, 1070
HspAI GCGC 1 cut(s) 129
Ksp22I TGATCA 5 cut(s) 6, 166, 870, 931, 1060
Kzo9I GATC 8 cut(s) 6, 166, 294, 352, 609, 870, 931, 1060
LguI GCTCTTC 1 cut(s) 28
LpnPI CCDG 8 cut(s) 61, 99, 348, 517, 594, 709, 964, 1033
Lsp1109I GCAGC 2 cut(s) 138, 467
LweI GCATC 3 cut(s) 269, 430, 484
MaeI CTAG 3 cut(s) 249, 855, 1190
MaeII ACGT 2 cut(s) 642, 1144
MaeIII GTNAC 2 cut(s) 953, 1022
MalI GATC 8 cut(s) 8, 168, 296, 354, 611, 872, 933, 1062
MboI GATC 8 cut(s) 6, 166, 294, 352, 609, 870, 931, 1060
MboII GAAGA 9 cut(s) 15, 18, 21, 24, 27, 30, 189, 197, 648
MfeI CAATTG 4 cut(s) 213, 690, 714, 1010
MflI RGATCY 2 cut(s) 294, 609
MhlI GDGCHC 1 cut(s) 606
MlsI TGGCCA 2 cut(s) 572, 845
MluNI TGGCCA 2 cut(s) 572, 845
MmeI TCCRAC 1 cut(s) 1070
Mox20I TGGCCA 2 cut(s) 572, 845
Mph1103I ATGCAT 1 cut(s) 262
MroXI GAANNNNTTC 1 cut(s) 1097
MscI TGGCCA 2 cut(s) 572, 845
MseI TTAA 5 cut(s) 525, 938, 972, 1032, 1037
MslI CAYNNNNRTG 1 cut(s) 158
Msp20I TGGCCA 2 cut(s) 572, 845
MspI CCGG 1 cut(s) 951
MunI CAATTG 4 cut(s) 213, 690, 714, 1010
Mva1269I GAATGC 2 cut(s) 416, 1099
MwoI GCNNNNNNNGC 2 cut(s) 123, 851
NcoI CCATGG 1 cut(s) 56
NdeI CATATG 1 cut(s) 648
NdeII GATC 8 cut(s) 6, 166, 294, 352, 609, 870, 931, 1060
NlaIII CATG 7 cut(s) 60, 71, 163, 241, 410, 518, 1070
NmuCI GTSAC 2 cut(s) 953, 1022
NsiI ATGCAT 1 cut(s) 262
PaeR7I CTCGAG 2 cut(s) 299, 1076
PagI TCATGA 3 cut(s) 406, 514, 1066
PceI AGGCCT 1 cut(s) 1167
PciSI GCTCTTC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 328
PctI GAATGC 2 cut(s) 416, 1099
PdmI GAANNNNTTC 1 cut(s) 1097
PfeI GAWTC 4 cut(s) 147, 417, 817, 861
PkrI GCNGC 2 cut(s) 128, 457
PsiI TTATAA 1 cut(s) 548
PspPI GGNCC 2 cut(s) 1048, 1112
PspXI VCTCGAGB 1 cut(s) 299
PsrI GAACNNNNNNTAC 2 cut(s) 1134, 1166
PsuI RGATCY 2 cut(s) 294, 609
RsaI GTAC 2 cut(s) 556, 578
RsaNI GTAC 2 cut(s) 555, 577
RseI CAYNNNNRTG 1 cut(s) 158
SapI GCTCTTC 1 cut(s) 28
SaqAI TTAA 5 cut(s) 525, 938, 972, 1032, 1037
SatI GCNGC 2 cut(s) 127, 456
Sau3AI GATC 8 cut(s) 6, 166, 294, 352, 609, 870, 931, 1060
Sau96I GGNCC 2 cut(s) 1048, 1112
SduI GDGCHC 1 cut(s) 606
SfaNI GCATC 3 cut(s) 269, 430, 484
SfcI CTRYAG 1 cut(s) 958
Sfr274I CTCGAG 2 cut(s) 299, 1076
SinI GGWCC 1 cut(s) 1048
SlaI CTCGAG 2 cut(s) 299, 1076
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 3 cut(s) 299, 1076, 1161
SmoI CTYRAG 3 cut(s) 299, 1076, 1161
SseBI AGGCCT 1 cut(s) 1167
SsiI CCGC 2 cut(s) 100, 1130
SspMI CTAG 3 cut(s) 249, 855, 1190
StuI AGGCCT 1 cut(s) 1167
StyI CCWWGG 1 cut(s) 56
TaaI ACNGT 4 cut(s) 222, 554, 959, 1028
TaiI ACGT 2 cut(s) 645, 1147
TaqI TCGA 4 cut(s) 224, 300, 331, 1077
TatI WGTACW 1 cut(s) 554
TfiI GAWTC 4 cut(s) 147, 417, 817, 861
Tru1I TTAA 5 cut(s) 525, 938, 972, 1032, 1037
Tru9I TTAA 5 cut(s) 525, 938, 972, 1032, 1037
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 2 cut(s) 953, 1022
TseI GCWGC 2 cut(s) 126, 455
Tsp45I GTSAC 2 cut(s) 953, 1022
TspDTI ATGAA 8 cut(s) 148, 297, 423, 503, 531, 635, 864, 1083
TspRI CASTG 1 cut(s) 511
VneI GTGCAC 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 1048
XapI RAATTY 5 cut(s) 310, 725, 763, 832, 1055
XbaI TCTAGA 1 cut(s) 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 274
XhoI CTCGAG 2 cut(s) 299, 1076
XmiI GTMKAC 1 cut(s) 1218
XmnI GAANNNNTTC 1 cut(s) 1097
XspI CTAG 3 cut(s) 249, 855, 1190
Zsp2I ATGCAT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.