Rroxscaffold_5G00358000

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0022728
Physical Location & Seq
Forward (+)
37971449 .. 37983253
11805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00358000.1

Sequence Viewer

Length: 1083 bp
ATGGTGATCATTGAAGAAGCCTCTTCTTCTTCTTCCACCCGTCCATGGAAATACCATGTCTTTTTGAGTTTTAGAGGTGAGGATACACGCTACAATTTCACAGACCATTTGTACAACGCTTTGCGTCGGGAGGGAATCATCACCTTTATGGATGATCAGCTCAGAAGAGGAGAAGAAATATCAACAGAACTTCTCTCCAAGCAATTGATCAGTCAACGATTTCAATCATCGTCTTCTCTAAAAACTATGCATCCTCCAAGTGGTGCTTGGAAGAACTTGTCAAGATCCTCGACTGCAAGAAATCAAATCAACAAGAGGTTCGACCAGTTTTCTACAAAGTCGATCCCTCGGATATACGAAATCACAGAGGTAGCTTTGGGGAGGCACTTGCTAATCATGAATGCAAATTCAAAGATAACCCGGACAAGGTCCGGAAATGGAAGGCAGCTCTTGAAGAAGCGGCAAATTTATCAGGGTGGCCTCTCTTGGATGAGTAAGCATTGTTCTGAATCCAGTGTTATTCATGAAATTGTTGAAGAGATTTCAGAGCAAGTGATAAGCAGTACATATTTGGATGTGGCTAAGTACCCAGTAGGAATAAAACCTCGGGTGCAACAGATCTGTAACCTTTTAGGTGTCGGGGGAAGAGATGTTCGTATGGTAGGGATATGGGGGCCCGGTGGAATAGGCAAGACAACAATTGCCAAAGCTGTCTATAACTCAATTGCTCATAAATTTGATGGGAGTTGTTTTTTGGAAAATGTTAGAGAAAATTCAATGGGTGCCAAAGGCTTTGTCAAACTACAAAAGAAACTTCTTTGTGAGATTCTAAAGGGAACAAAATTGAAGGTGACCAATGTAGCAAGAGGAATCACTATGATTAAGGAAAGGTTGCAATACAAAAGTGTTCTCTTAGTTCTTGATGATGTGAATGACATGGATCAGATAGATAACTTAGCTGGTCAATGTAGTTGGTTTGGAATGGGTAGTAGAATCATCATAACAACAAGGGATAAGCAATTGCTGAGGCATCATAATGTTAATAACTTAATATACGAGGTCCAGAAATTGGGATCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

360

Amino Acids

40.79

Weight (kDa)

9.78

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 17 - 66 8.4e-12 TIR domain
NB-ARC PF00931 204 - 349 2e-17 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022728)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0411741
rosa_multiflora Rmu_co8266463.1_g000001 Rmu_sc0000711.1_g000029
rosa_roxburghii Rroxscaffold_5G00358000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 782
AccB7I CCANNNNNTGG 1 cut(s) 1069
AccIII TCCGGA 1 cut(s) 431
AciI CCGC 1 cut(s) 460
AclWI GGATC 4 cut(s) 279, 337, 948, 1081
AcsI RAATTY 4 cut(s) 406, 465, 734, 772
AfaI GTAC 3 cut(s) 113, 565, 587
AfiI CCNNNNNNNGG 4 cut(s) 45, 260, 426, 1069
AgsI TTSAA 7 cut(s) 14, 224, 411, 454, 536, 777, 847
AloI GAACNNNNNNTCC 2 cut(s) 636, 668
AluBI AGCT 5 cut(s) 160, 374, 448, 710, 959
AluI AGCT 5 cut(s) 160, 374, 448, 710, 959
AlwI GGATC 4 cut(s) 279, 337, 948, 1081
Ama87I CYCGRG 1 cut(s) 606
Aor13HI TCCGGA 1 cut(s) 431
AoxI GGCC 2 cut(s) 478, 674
ApaI GGGCCC 1 cut(s) 678
ApeKI GCWGC 1 cut(s) 445
ApoI RAATTY 4 cut(s) 406, 465, 734, 772
ArsI GACNNNNNNTTYG 2 cut(s) 780, 812
AspS9I GGNCC 4 cut(s) 429, 674, 675, 1060
AsuC2I CCSGG 2 cut(s) 421, 678
AsuHPI GGTGA 4 cut(s) 16, 89, 133, 862
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 2 cut(s) 429, 1060
BaeGI GKGCMC 1 cut(s) 678
BanI GGYRCC 1 cut(s) 782
BanII GRGCYC 1 cut(s) 678
BbsI GAAGAC 1 cut(s) 225
BbvCI CCTCAGC 1 cut(s) 1025
BbvI GCAGC 1 cut(s) 457
BccI CCATC 1 cut(s) 734
BciVI GTATCC 1 cut(s) 76
BclI TGATCA 3 cut(s) 6, 154, 207
BcnI CCSGG 2 cut(s) 421, 678
BfuI GTATCC 1 cut(s) 76
BglII AGATCT 1 cut(s) 618
BisI GCNGC 2 cut(s) 446, 461
BlsI GCNGC 2 cut(s) 447, 462
Bme1390I CCNGG 2 cut(s) 421, 678
Bme18I GGWCC 2 cut(s) 429, 1060
BmeT110I CYCGRG 1 cut(s) 606
BmgT120I GGNCC 4 cut(s) 429, 674, 675, 1060
BmiI GGNNCC 3 cut(s) 675, 676, 784
BmrFI CCNGG 2 cut(s) 421, 678
BmrI ACTGGG 1 cut(s) 584
BmsI GCATC 2 cut(s) 259, 1039
BmuI ACTGGG 1 cut(s) 584
BpiI GAAGAC 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 1025
BpuMI CCSGG 2 cut(s) 421, 678
BsaBI GATNNNNATC 1 cut(s) 223
BsaJI CCNNGG 3 cut(s) 44, 347, 605
BsaWI WCCGGW 1 cut(s) 431
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 260, 426, 1069
Bse1I ACTGG 3 cut(s) 325, 513, 590
Bse8I GATNNNNATC 1 cut(s) 223
BseAI TCCGGA 1 cut(s) 431
BseDI CCNNGG 3 cut(s) 44, 347, 605
BseGI GGATG 4 cut(s) 157, 250, 495, 580
BseJI GATNNNNATC 1 cut(s) 223
BseLI CCNNNNNNNGG 4 cut(s) 45, 260, 426, 1069
BseMII CTCAG 2 cut(s) 175, 1016
BseNI ACTGG 3 cut(s) 325, 513, 590
BseRI GAGGAG 1 cut(s) 183
BseSI GKGCMC 1 cut(s) 678
BseXI GCAGC 1 cut(s) 457
BshFI GGCC 2 cut(s) 480, 676
BshNI GGYRCC 1 cut(s) 782
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 3 cut(s) 421, 432, 678
BslI CCNNNNNNNGG 4 cut(s) 45, 260, 426, 1069
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 2 cut(s) 480, 676
BsoBI CYCGRG 1 cut(s) 606
Bsp120I GGGCCC 1 cut(s) 674
Bsp1286I GDGCHC 1 cut(s) 678
Bsp13I TCCGGA 1 cut(s) 431
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 8 cut(s) 6, 154, 207, 284, 342, 618, 940, 1073
Bsp19I CCATGG 1 cut(s) 44
BspACI CCGC 1 cut(s) 460
BspANI GGCC 2 cut(s) 480, 676
BspCNI CTCAG 2 cut(s) 174, 1017
BspEI TCCGGA 1 cut(s) 431
BspHI TCATGA 3 cut(s) 396, 523, 1079
BspLI GGNNCC 3 cut(s) 675, 676, 784
BspPI GGATC 4 cut(s) 279, 337, 948, 1081
BspT107I GGYRCC 1 cut(s) 782
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 3 cut(s) 325, 513, 590
BssECI CCNNGG 3 cut(s) 44, 347, 605
BssMI GATC 8 cut(s) 6, 154, 207, 284, 342, 618, 940, 1073
BssT1I CCWWGG 1 cut(s) 44
Bst6I CTCTTC 4 cut(s) 28, 160, 531, 640
BstAUI TGTACA 1 cut(s) 111
BstDEI CTNAG 5 cut(s) 161, 582, 913, 955, 1025
BstDSI CCRYGG 1 cut(s) 44
BstEII GGTNACC 1 cut(s) 850
BstF5I GGATG 4 cut(s) 157, 250, 495, 580
BstKTI GATC 8 cut(s) 9, 157, 210, 287, 345, 621, 943, 1076
BstMBI GATC 8 cut(s) 6, 154, 207, 284, 342, 618, 940, 1073
BstPI GGTNACC 1 cut(s) 850
BstSCI CCNGG 2 cut(s) 419, 676
BstSLI GKGCMC 1 cut(s) 678
BstV1I GCAGC 1 cut(s) 457
BstV2I GAAGAC 1 cut(s) 225
BstX2I RGATCY 2 cut(s) 284, 618
BstYI RGATCY 2 cut(s) 284, 618
BsuI GTATCC 1 cut(s) 76
BsuRI GGCC 2 cut(s) 480, 676
BtgI CCRYGG 1 cut(s) 44
BtsCI GGATG 4 cut(s) 157, 250, 495, 580
BtsIMutI CAGTG 1 cut(s) 520
CciI TCATGA 3 cut(s) 396, 523, 1079
Cfr13I GGNCC 4 cut(s) 429, 674, 675, 1060
CseI GACGC 1 cut(s) 113
Csp6I GTAC 3 cut(s) 112, 564, 586
CviAII CATG 6 cut(s) 45, 56, 397, 524, 937, 1080
CviQI GTAC 3 cut(s) 112, 564, 586
DdeI CTNAG 5 cut(s) 161, 582, 913, 955, 1025
DpnI GATC 8 cut(s) 8, 156, 209, 286, 344, 620, 942, 1075
DpnII GATC 8 cut(s) 6, 154, 207, 284, 342, 618, 940, 1073
Eam1104I CTCTTC 4 cut(s) 28, 160, 531, 640
EarI CTCTTC 4 cut(s) 28, 160, 531, 640
Eco130I CCWWGG 1 cut(s) 44
Eco24I GRGCYC 1 cut(s) 678
Eco47I GGWCC 2 cut(s) 429, 1060
Eco88I CYCGRG 1 cut(s) 606
Eco91I GGTNACC 1 cut(s) 850
EcoO109I RGGNCCY 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 850
EcoT14I CCWWGG 1 cut(s) 44
EcoT22I ATGCAT 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 6 cut(s) 48, 59, 400, 527, 940, 1083
FalI AAGNNNNNCTT 2 cut(s) 250, 282
FatI CATG 6 cut(s) 44, 55, 396, 523, 936, 1079
FbaI TGATCA 3 cut(s) 6, 154, 207
Fnu4HI GCNGC 2 cut(s) 446, 461
FokI GGATG 4 cut(s) 164, 237, 502, 587
FriOI GRGCYC 1 cut(s) 678
Fsp4HI GCNGC 2 cut(s) 446, 461
GluI GCNGC 2 cut(s) 446, 461
HaeIII GGCC 2 cut(s) 480, 676
HapII CCGG 3 cut(s) 421, 432, 678
HgaI GACGC 1 cut(s) 113
Hin1II CATG 6 cut(s) 48, 59, 400, 527, 940, 1083
HincII GTYRAC 1 cut(s) 215
HindII GTYRAC 1 cut(s) 215
HinfI GANTC 5 cut(s) 135, 509, 826, 870, 993
HpaII CCGG 3 cut(s) 421, 432, 678
HphI GGTGA 4 cut(s) 16, 89, 133, 862
Hpy166II GTNNAC 1 cut(s) 215
Hpy188I TCNGA 5 cut(s) 164, 351, 508, 547, 945
Hpy188III TCNNGA 9 cut(s) 128, 282, 397, 432, 451, 524, 920, 1063, 1080
Hpy8I GTNNAC 1 cut(s) 215
Hpy99I CGWCG 1 cut(s) 129
HpyAV CCTTC 2 cut(s) 435, 841
HpyCH4V TGCA 5 cut(s) 250, 296, 404, 613, 895
HpyF3I CTNAG 5 cut(s) 161, 582, 913, 955, 1025
Hsp92II CATG 6 cut(s) 48, 59, 400, 527, 940, 1083
Kpn2I TCCGGA 1 cut(s) 431
Ksp22I TGATCA 3 cut(s) 6, 154, 207
Kzo9I GATC 8 cut(s) 6, 154, 207, 284, 342, 618, 940, 1073
LpnPI CCDG 9 cut(s) 338, 434, 445, 458, 526, 603, 691, 945, 1076
Lsp1109I GCAGC 1 cut(s) 457
LweI GCATC 2 cut(s) 259, 1039
MaeIII GTNAC 2 cut(s) 623, 850
MalI GATC 8 cut(s) 8, 156, 209, 286, 344, 620, 942, 1075
MboI GATC 8 cut(s) 6, 154, 207, 284, 342, 618, 940, 1073
MfeI CAATTG 4 cut(s) 203, 699, 723, 1019
MflI RGATCY 2 cut(s) 284, 618
MhlI GDGCHC 1 cut(s) 678
Mph1103I ATGCAT 1 cut(s) 252
MroI TCCGGA 1 cut(s) 431
MseI TTAA 3 cut(s) 882, 1041, 1049
MslI CAYNNNNRTG 2 cut(s) 146, 1035
MspI CCGG 3 cut(s) 421, 432, 678
MspR9I CCNGG 2 cut(s) 421, 678
MunI CAATTG 4 cut(s) 203, 699, 723, 1019
Mva1269I GAATGC 1 cut(s) 406
NciI CCSGG 2 cut(s) 421, 678
NcoI CCATGG 1 cut(s) 44
NdeII GATC 8 cut(s) 6, 154, 207, 284, 342, 618, 940, 1073
NlaIII CATG 6 cut(s) 48, 59, 400, 527, 940, 1083
NlaIV GGNNCC 3 cut(s) 675, 676, 784
NmuCI GTSAC 1 cut(s) 850
NsiI ATGCAT 1 cut(s) 252
PagI TCATGA 3 cut(s) 396, 523, 1079
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 5 cut(s) 135, 509, 826, 870, 993
PflFI GACNNNGTC 1 cut(s) 427
PflMI CCANNNNNTGG 1 cut(s) 1069
PkrI GCNGC 2 cut(s) 447, 462
PspEI GGTNACC 1 cut(s) 850
PspN4I GGNNCC 3 cut(s) 675, 676, 784
PspOMI GGGCCC 1 cut(s) 674
PspPI GGNCC 4 cut(s) 429, 674, 675, 1060
PsrI GAACNNNNNNTAC 2 cut(s) 487, 519
PsuI RGATCY 2 cut(s) 284, 618
PsyI GACNNNGTC 1 cut(s) 427
RsaI GTAC 3 cut(s) 113, 565, 587
RsaNI GTAC 3 cut(s) 112, 564, 586
RseI CAYNNNNRTG 2 cut(s) 146, 1035
SaqAI TTAA 3 cut(s) 882, 1041, 1049
SatI GCNGC 2 cut(s) 446, 461
Sau3AI GATC 8 cut(s) 6, 154, 207, 284, 342, 618, 940, 1073
Sau96I GGNCC 4 cut(s) 429, 674, 675, 1060
ScrFI CCNGG 2 cut(s) 421, 678
SduI GDGCHC 1 cut(s) 678
SfaNI GCATC 2 cut(s) 259, 1039
SinI GGWCC 2 cut(s) 429, 1060
SmiMI CAYNNNNRTG 2 cut(s) 146, 1035
SsiI CCGC 1 cut(s) 460
StyD4I CCNGG 2 cut(s) 419, 676
StyI CCWWGG 1 cut(s) 44
TaqI TCGA 3 cut(s) 290, 321, 341
TatI WGTACW 2 cut(s) 111, 563
TauI GCSGC 1 cut(s) 463
TfiI GAWTC 5 cut(s) 135, 509, 826, 870, 993
Tru1I TTAA 3 cut(s) 882, 1041, 1049
Tru9I TTAA 3 cut(s) 882, 1041, 1049
TscAI CASTG 1 cut(s) 520
TseFI GTSAC 1 cut(s) 850
TseI GCWGC 1 cut(s) 445
Tsp45I GTSAC 1 cut(s) 850
TspDTI ATGAA 3 cut(s) 413, 512, 540
TspRI CASTG 1 cut(s) 520
Tth111I GACNNNGTC 1 cut(s) 427
Van91I CCANNNNNTGG 1 cut(s) 1069
VpaK11BI GGWCC 2 cut(s) 429, 1060
XapI RAATTY 4 cut(s) 406, 465, 734, 772
XcmI CCANNNNNNNNNTGG 1 cut(s) 264
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.