Rmu_co8266463.1_g000001

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8266463.1
OG0022728
Physical Location & Seq
Forward (+)
182 .. 819
638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8266463.1_g000001.1.cds

Sequence Viewer

Length: 638 bp
atgtccaggcatcattcggaatccagttttattcatgacatcgttggagagatttcaaaggaagtcataagccatacatatttggatgtggccaagtacccagttggaataaaacgtcgtgtgcaaaagatccatgagcttttatatgtcaagggaagagacgttcatatggtagggatatggggggctggtggaattggtaagacaacaaatgctaaagctgtctataactcaattgctcctaaatttgaagggatttgttttctggaaatgggtgccaaaggccttgtcaaacttcaaaagacacttcttcgtgagattctaaaggaaacaaaattgaaggtgggcaatgtagctagaggaattactatgatcaaggaaaggttgcagtacaaaagagttcttttagttcttgatgatgtgaatgacatggatcagttaaacaagttggccggtgaccctagttggtttggtatgggtagtaaaatcatcataacaacaagggataagaaattgcttagaggtcatggtgttaatttgatatacgaggtcaaggcattggaccatgatgaagctctcgaccttttcagtatgaatgcctttaaaagaagtcaaccttcagatggttatgtagaact
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

213

Amino Acids

23.98

Weight (kDa)

9.44

Isoelectric Point (pI)

25.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022728)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0411741
rosa_multiflora Rmu_co8266463.1_g000001 Rmu_sc0000711.1_g000029
rosa_roxburghii Rroxscaffold_5G00358000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 275
AclWI GGATC 2 cut(s) 124, 441
AcoI YGGCCR 2 cut(s) 90, 450
AcsI RAATTY 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 603
AfaI GTAC 2 cut(s) 98, 392
AfiI CCNNNNNNNGG 1 cut(s) 623
AgsI TTSAA 4 cut(s) 57, 251, 299, 340
AjnI CCWGG 1 cut(s) 5
AloI GAACNNNNNNTCC 2 cut(s) 147, 179
AluBI AGCT 4 cut(s) 139, 221, 356, 575
AluI AGCT 4 cut(s) 139, 221, 356, 575
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 2 cut(s) 124, 441
AoxI GGCC 3 cut(s) 90, 283, 450
ApoI RAATTY 1 cut(s) 245
ArsI GACNNNNNNTTYG 2 cut(s) 273, 305
AspS9I GGNCC 1 cut(s) 562
AsuHPI GGTGA 1 cut(s) 467
AvaII GGWCC 1 cut(s) 562
BalI TGGCCA 1 cut(s) 92
BanI GGYRCC 1 cut(s) 275
BccI CCATC 1 cut(s) 617
BciT130I CCWGG 1 cut(s) 7
BclI TGATCA 1 cut(s) 372
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 2 cut(s) 357, 462
Bme1390I CCNGG 1 cut(s) 7
Bme18I GGWCC 1 cut(s) 562
BmgT120I GGNCC 1 cut(s) 562
BmiI GGNNCC 1 cut(s) 277
BmrFI CCNGG 1 cut(s) 7
BmrI ACTGGG 1 cut(s) 95
BmsI GCATC 1 cut(s) 19
BmuI ACTGGG 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 623
Bse118I RCCGGY 1 cut(s) 452
Bse1I ACTGG 2 cut(s) 24, 101
Bse3DI GCAATG 1 cut(s) 355
BseBI CCWGG 1 cut(s) 7
BseGI GGATG 1 cut(s) 91
BseLI CCNNNNNNNGG 1 cut(s) 623
BseMI GCAATG 1 cut(s) 355
BseNI ACTGG 2 cut(s) 24, 101
BshFI GGCC 3 cut(s) 92, 285, 452
BshNI GGYRCC 1 cut(s) 275
BsiSI CCGG 1 cut(s) 453
BslI CCNNNNNNNGG 1 cut(s) 623
BsmAI GTCTC 1 cut(s) 153
BsmBI CGTCTC 1 cut(s) 153
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 3 cut(s) 92, 285, 452
Bsp143I GATC 3 cut(s) 129, 372, 433
BspANI GGCC 3 cut(s) 92, 285, 452
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 277
BspPI GGATC 2 cut(s) 124, 441
BspT107I GGYRCC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 355
BsrFI RCCGGY 1 cut(s) 452
BsrI ACTGG 2 cut(s) 24, 101
BssAI RCCGGY 1 cut(s) 452
BssMI GATC 3 cut(s) 129, 372, 433
Bst2UI CCWGG 1 cut(s) 7
Bst6I CTCTTC 1 cut(s) 151
BstDEI CTNAG 1 cut(s) 518
BstEII GGTNACC 1 cut(s) 455
BstF5I GGATG 1 cut(s) 91
BstKTI GATC 3 cut(s) 132, 375, 436
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 3 cut(s) 129, 372, 433
BstNI CCWGG 1 cut(s) 7
BstPI GGTNACC 1 cut(s) 455
BstSCI CCNGG 1 cut(s) 5
BstX2I RGATCY 1 cut(s) 129
BstYI RGATCY 1 cut(s) 129
BsuRI GGCC 3 cut(s) 92, 285, 452
BtsCI GGATG 1 cut(s) 91
CciI TCATGA 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 1 cut(s) 562
Csp6I GTAC 2 cut(s) 97, 391
CviAII CATG 5 cut(s) 35, 134, 430, 527, 566
CviJI RGCY 9 cut(s) 72, 92, 139, 188, 221, 285, 356, 452, 575
CviKI_1 RGCY 9 cut(s) 72, 92, 139, 188, 221, 285, 356, 452, 575
CviQI GTAC 2 cut(s) 97, 391
DdeI CTNAG 1 cut(s) 518
DpnI GATC 3 cut(s) 131, 374, 435
DpnII GATC 3 cut(s) 129, 372, 433
DraI TTTAAA 1 cut(s) 604
EaeI YGGCCR 2 cut(s) 90, 450
Eam1104I CTCTTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 151
Eco147I AGGCCT 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 562
Eco57I CTGAAG 1 cut(s) 603
Eco91I GGTNACC 1 cut(s) 455
EcoO65I GGTNACC 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 5
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 5 cut(s) 38, 137, 433, 530, 569
FalI AAGNNNNNCTT 2 cut(s) 601, 633
FatI CATG 5 cut(s) 34, 133, 429, 526, 565
FauNDI CATATG 1 cut(s) 168
FbaI TGATCA 1 cut(s) 372
FokI GGATG 1 cut(s) 98
FspBI CTAG 2 cut(s) 357, 462
HaeIII GGCC 3 cut(s) 92, 285, 452
HapII CCGG 1 cut(s) 453
Hin1II CATG 5 cut(s) 38, 137, 433, 530, 569
HincII GTYRAC 1 cut(s) 614
HindII GTYRAC 1 cut(s) 614
HinfI GANTC 2 cut(s) 20, 319
HpaII CCGG 1 cut(s) 453
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 1 cut(s) 614
Hpy188I TCNGA 2 cut(s) 19, 622
Hpy188III TCNNGA 5 cut(s) 35, 266, 314, 413, 578
Hpy8I GTNNAC 1 cut(s) 614
Hpy99I CGWCG 1 cut(s) 120
HpyAV CCTTC 3 cut(s) 245, 334, 627
HpyCH4IV ACGT 2 cut(s) 115, 162
HpyCH4V TGCA 2 cut(s) 124, 388
HpyF3I CTNAG 1 cut(s) 518
HpySE526I ACGT 2 cut(s) 115, 162
Hsp92II CATG 5 cut(s) 38, 137, 433, 530, 569
Ksp22I TGATCA 1 cut(s) 372
Kzo9I GATC 3 cut(s) 129, 372, 433
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 6 cut(s) 19, 37, 114, 174, 251, 466
LweI GCATC 1 cut(s) 19
MaeI CTAG 2 cut(s) 357, 462
MaeII ACGT 2 cut(s) 115, 162
MaeIII GTNAC 1 cut(s) 455
MalI GATC 3 cut(s) 131, 374, 435
MboI GATC 3 cut(s) 129, 372, 433
MboII GAAGA 2 cut(s) 168, 302
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 1 cut(s) 129
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 7 cut(s) 195, 234, 245, 335, 363, 512, 535
MluNI TGGCCA 1 cut(s) 92
MmeI TCCRAC 2 cut(s) 25, 85
MnlI CCTC 3 cut(s) 353, 515, 541
Mox20I TGGCCA 1 cut(s) 92
MscI TGGCCA 1 cut(s) 92
MseI TTAA 3 cut(s) 440, 534, 603
Msp20I TGGCCA 1 cut(s) 92
MspI CCGG 1 cut(s) 453
MspR9I CCNGG 1 cut(s) 7
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 1 cut(s) 601
MvaI CCWGG 1 cut(s) 7
NdeI CATATG 1 cut(s) 168
NdeII GATC 3 cut(s) 129, 372, 433
NlaIII CATG 5 cut(s) 38, 137, 433, 530, 569
NlaIV GGNNCC 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 455
PagI TCATGA 1 cut(s) 34
PceI AGGCCT 1 cut(s) 285
PctI GAATGC 1 cut(s) 601
PfeI GAWTC 2 cut(s) 20, 319
Psp6I CCWGG 1 cut(s) 5
PspEI GGTNACC 1 cut(s) 455
PspGI CCWGG 1 cut(s) 5
PspN4I GGNNCC 1 cut(s) 277
PspPI GGNCC 1 cut(s) 562
PsuI RGATCY 1 cut(s) 129
RsaI GTAC 2 cut(s) 98, 392
RsaNI GTAC 2 cut(s) 97, 391
SaqAI TTAA 3 cut(s) 440, 534, 603
Sau3AI GATC 3 cut(s) 129, 372, 433
Sau96I GGNCC 1 cut(s) 562
ScrFI CCNGG 1 cut(s) 7
SfaNI GCATC 1 cut(s) 19
SinI GGWCC 1 cut(s) 562
Sse9I AATT 7 cut(s) 195, 234, 245, 335, 363, 512, 535
SseBI AGGCCT 1 cut(s) 285
SspMI CTAG 2 cut(s) 357, 462
StuI AGGCCT 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 5
TaiI ACGT 2 cut(s) 118, 165
TaqI TCGA 1 cut(s) 579
TasI AATT 7 cut(s) 195, 234, 245, 335, 363, 512, 535
TatI WGTACW 1 cut(s) 390
TfiI GAWTC 2 cut(s) 20, 319
Tru1I TTAA 3 cut(s) 440, 534, 603
Tru9I TTAA 3 cut(s) 440, 534, 603
TseFI GTSAC 1 cut(s) 455
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 4 cut(s) 23, 155, 585, 608
VpaK11BI GGWCC 1 cut(s) 562
XapI RAATTY 1 cut(s) 245
XspI CTAG 2 cut(s) 357, 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.