RchiOBHm_Chr4g0421441

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009120
Physical Location & Seq
Reverse (-)
47104594 .. 47111770
7177 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39109

Sequence Viewer

Length: 1683 bp
ATGGACGTCCCCAAGGATCAAATCGCTACTCTTATGGAGCATGGCCTCTACACCTCGGCTCAAATGCTCGGTTGCTTTCTTGTTTCTTCACCAGCTGCTAATGCCGAAGTCACTCCGCACCTCAAAGCTGATAGCCTGGTGCTTCTTGGAGATTCGCTGTTTCGGGAAAGGGAGTTTCGCAGAGCAATTCATACATACAAGCAAGCATTGCAAGTTTGCAAGCTTATTTCCAAAACAAATCCAACATCGTCGAGAAGTTCATTGGTCTCAAATCGGTCATCGTCTCCAAATGTTTTTAACATTTCAGGAATTAATGAGAATGAGGTGAAGTTCAAAATTGCGTCTTGTCAATGTGCGCTTAGTGAGTACGGAGCAGCCCTTGCTGAGATGGATAATATTCCAAGCAAAATGAGAAACTTGCAGATGAATTTGTTGATGGGAAAACTCTATCGGTATTCTAGACAGAATCGTTCTGCTGTTTCTTGTTTCAAAGAATGTCTAAGGTCTTGCCCTTATGTCTTCGAGGCTATCATAGCTTTGGCAGAATTAGGAGTTTCTGCTAAAGACATTATTTCCTTGATTCCGCAGACTCCAAATAGAAGTGGGAGGGCATCATTTGATCATTTGGACTCGAGTCGTTGGCTGCCACGCTATGTTGAGGCCCAGTGTTCCATTGCTTCTAATGATTACAAAGGTGGTCTGGAACTCTTTACAGATCTTTTGCAACGTTTCCCGAACAATTTACACTTGCTACTTGAAATTGCAAAGGTTGAAGCTATTGTTGGGAAGAATGATGAGGCTATAATGAATTTTGATAAGGCTCGATTAATTGATCCATATGTTGTGACATATATGGATGAGTATGCAATGCTTCTGAAGATAAAGTCTGACTTTTCAAAGCTAAACAAATTAGTGCATGATTTGTTGATCACTGACTCTATGAGGCCAGAAGTTTTTGTGGCCTTATCTGTGCTTTGGGAAAGAAAAGATGAGAGAGGAGCTTTATCTTATGCTGAGAAGAGCATACGTATCGACGAGAGGCATATACCAGGTTATATAATGAAGGGTAACCTGCTGTTATCAATGAAGCGAGCAGAAGCAGCTGTAAGTGCCTTTAGGGCAGCTCAAGAGTTGAGACCTGATATTCGTTCATATCAAGGTTTGGTTCAGTCTTATCTAGCTCTCTCTAAAATTAAAGAGGCTTTATATGCTTCAAGGGAGGCTATGAAGGCCATGCCTCAATCTGCGAAGGCTCTAAAATTAGTAGGGGATGTACATGCGAGTAACTCTGGTGGCAGGGAGAAGGCAAAAAAGTTCTATGAGTCAGCCCTTAGGCTGGAACCTGGTTATCTCGGAGCTGCATTAGCTCTGGCTGAGCTCCATGTTATTGAAGGCCGTAACGGGGATGCTGTGTCTCTGCTAGAGCGATATCTCAAGGATTGGGCCGATGACTCTCTACATGTCAAGCTCGCTCAAGTATTTGCTGCAACAAATATGCTGCAAGATGCATTATCACATTTTCAAGCTGCATTGAGGATAAATGCACAGAATGAAGCTGCAAAAAAAGGTTTAGAGCGCTTAGAGAAACAAATGAAGGGGGTGGATCCTGATGCACCTGAAGAAGATGAAGATAACGAGGTCGATGATGCTGATGGAGACCAAGATGAGACTGAACTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166
Pfam Domains
Protein Families
TPR_16 TPR_19 TPR_7 TPR_8

Protein Analysis

560

Amino Acids

62.48

Weight (kDa)

6.08

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 9
Acc36I ACCTGC 1 cut(s) 1080
AciI CCGC 2 cut(s) 116, 584
AclI AACGTT 1 cut(s) 727
AclWI GGATC 4 cut(s) 24, 827, 1598, 1611
AcsI RAATTY 2 cut(s) 427, 808
AcuI CTGAAG 2 cut(s) 896, 1638
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 2 cut(s) 368, 1275
AfeI AGCGCT 1 cut(s) 1577
AfiI CCNNNNNNNGG 2 cut(s) 1336, 1402
AflIII ACRYGT 1 cut(s) 1459
AgsI TTSAA 8 cut(s) 334, 490, 758, 773, 897, 1215, 1391, 1523
AjnI CCWGG 3 cut(s) 135, 1048, 1342
AjuI GAANNNNNNNTTGG 2 cut(s) 765, 797
Alw21I GWGCWC 1 cut(s) 1380
Alw26I GTCTC 6 cut(s) 271, 288, 1129, 1419, 1650, 1661
AlwI GGATC 4 cut(s) 24, 827, 1598, 1611
Ama87I CYCGRG 1 cut(s) 631
Aor51HI AGCGCT 1 cut(s) 1577
AoxI GGCC 7 cut(s) 43, 660, 944, 960, 1230, 1393, 1443
ApoI RAATTY 2 cut(s) 427, 808
ArsI GACNNNNNNTTYG 2 cut(s) 1128, 1160
AseI ATTAAT 2 cut(s) 312, 827
AspLEI GCGC 2 cut(s) 358, 1578
AspS9I GGNCC 2 cut(s) 661, 1443
AsuHPI GGTGA 2 cut(s) 81, 337
AvaI CYCGRG 1 cut(s) 631
AxyI CCTNAGG 1 cut(s) 1331
BamHI GGATCC 1 cut(s) 1603
BanII GRGCYC 1 cut(s) 1380
BbsI GAAGAC 1 cut(s) 511
Bbv12I GWGCWC 1 cut(s) 1380
BccI CCATC 3 cut(s) 382, 430, 1646
BceAI ACGGC 1 cut(s) 1380
BcgI CGANNNNNNTGC 2 cut(s) 1012, 1046
BciT130I CCWGG 3 cut(s) 137, 1050, 1344
BclI TGATCA 2 cut(s) 619, 927
BcoDI GTCTC 6 cut(s) 271, 288, 1129, 1419, 1650, 1661
BfaI CTAG 3 cut(s) 459, 1178, 1421
BfoI RGCGCY 1 cut(s) 1579
BfuAI ACCTGC 1 cut(s) 1080
BglI GCCNNNNNGGC 1 cut(s) 1118
BglII AGATCT 1 cut(s) 715
BlpI GCTNAGC 1 cut(s) 1374
Bme1390I CCNGG 3 cut(s) 137, 1050, 1344
BmeT110I CYCGRG 1 cut(s) 631
BmgT120I GGNCC 2 cut(s) 661, 1443
BmiI GGNNCC 2 cut(s) 1341, 1605
BmrFI CCNGG 3 cut(s) 137, 1050, 1344
BmrI ACTGGG 1 cut(s) 658
BmsI GCATC 5 cut(s) 620, 1396, 1495, 1600, 1636
BmuI ACTGGG 1 cut(s) 658
BoxI GACNNNNGTC 1 cut(s) 633
BpiI GAAGAC 1 cut(s) 511
Bpu1102I GCTNAGC 1 cut(s) 1374
BpuEI CTTGAG 3 cut(s) 1110, 1418, 1458
BsaAI YACGTR 1 cut(s) 1028
BsaHI GRCGYC 1 cut(s) 6
BsaI GGTCTC 3 cut(s) 271, 1129, 1650
BsaJI CCNNGG 2 cut(s) 12, 54
Bsc4I CCNNNNNNNGG 2 cut(s) 1336, 1402
Bse1I ACTGG 1 cut(s) 664
Bse21I CCTNAGG 1 cut(s) 1331
Bse3DI GCAATG 3 cut(s) 206, 672, 873
BseBI CCWGG 3 cut(s) 137, 1050, 1344
BseDI CCNNGG 2 cut(s) 12, 54
BseGI GGATG 3 cut(s) 862, 1276, 1411
BseLI CCNNNNNNNGG 2 cut(s) 1336, 1402
BseMI GCAATG 3 cut(s) 206, 672, 873
BseMII CTCAG 3 cut(s) 375, 1005, 1365
BseNI ACTGG 1 cut(s) 664
BseRI GAGGAG 1 cut(s) 1011
BshFI GGCC 7 cut(s) 45, 662, 946, 962, 1232, 1395, 1445
BsiHKAI GWGCWC 1 cut(s) 1380
BsiHKCI CYCGRG 1 cut(s) 631
BslI CCNNNNNNNGG 2 cut(s) 1336, 1402
BsmAI GTCTC 6 cut(s) 271, 288, 1129, 1419, 1650, 1661
BsmBI CGTCTC 1 cut(s) 288
BsnI GGCC 7 cut(s) 45, 662, 946, 962, 1232, 1395, 1445
Bso31I GGTCTC 3 cut(s) 271, 1129, 1650
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 1 cut(s) 1380
Bsp1407I TGTACA 1 cut(s) 1273
Bsp143I GATC 6 cut(s) 16, 619, 715, 832, 927, 1603
Bsp1720I GCTNAGC 1 cut(s) 1374
BspACI CCGC 2 cut(s) 116, 584
BspANI GGCC 7 cut(s) 45, 662, 946, 962, 1232, 1395, 1445
BspCNI CTCAG 3 cut(s) 376, 1006, 1366
BspLI GGNNCC 2 cut(s) 1341, 1605
BspMI ACCTGC 1 cut(s) 1080
BspPI GGATC 4 cut(s) 24, 827, 1598, 1611
BspQI GCTCTTC 1 cut(s) 1013
BspTNI GGTCTC 3 cut(s) 271, 1129, 1650
BsrDI GCAATG 3 cut(s) 206, 672, 873
BsrGI TGTACA 1 cut(s) 1273
BsrI ACTGG 1 cut(s) 664
BssECI CCNNGG 2 cut(s) 12, 54
BssMI GATC 6 cut(s) 16, 619, 715, 832, 927, 1603
BssNI GRCGYC 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 3 cut(s) 137, 1050, 1344
Bst6I CTCTTC 1 cut(s) 1013
BstACI GRCGYC 1 cut(s) 6
BstAPI GCANNNNNTGC 2 cut(s) 208, 380
BstAUI TGTACA 1 cut(s) 1273
BstBAI YACGTR 1 cut(s) 1028
BstC8I GCNNGC 4 cut(s) 204, 221, 1092, 1470
BstDEI CTNAG 7 cut(s) 359, 384, 500, 1014, 1331, 1374, 1579
BstEII GGTNACC 1 cut(s) 1067
BstF5I GGATG 3 cut(s) 862, 1276, 1411
BstH2I RGCGCY 1 cut(s) 1579
BstHHI GCGC 2 cut(s) 358, 1578
BstKTI GATC 6 cut(s) 19, 622, 718, 835, 930, 1606
BstMAI GTCTC 6 cut(s) 271, 288, 1129, 1419, 1650, 1661
BstMBI GATC 6 cut(s) 16, 619, 715, 832, 927, 1603
BstNI CCWGG 3 cut(s) 137, 1050, 1344
BstNSI RCATGY 2 cut(s) 1280, 1463
BstPAI GACNNNNGTC 1 cut(s) 633
BstPI GGTNACC 1 cut(s) 1067
BstSCI CCNGG 3 cut(s) 135, 1048, 1342
BstSNI TACGTA 1 cut(s) 1028
BstV2I GAAGAC 1 cut(s) 511
BstX2I RGATCY 2 cut(s) 715, 1603
BstYI RGATCY 2 cut(s) 715, 1603
Bsu36I CCTNAGG 1 cut(s) 1331
BsuRI GGCC 7 cut(s) 45, 662, 946, 962, 1232, 1395, 1445
BtsCI GGATG 3 cut(s) 862, 1276, 1411
BtsIMutI CAGTG 2 cut(s) 671, 930
BveI ACCTGC 1 cut(s) 1080
Cac8I GCNNGC 4 cut(s) 204, 221, 1092, 1470
CfoI GCGC 2 cut(s) 358, 1578
Cfr13I GGNCC 2 cut(s) 661, 1443
CseI GACGC 1 cut(s) 330
CsiI ACCWGGT 2 cut(s) 1048, 1342
Csp6I GTAC 2 cut(s) 367, 1274
CviAII CATG 6 cut(s) 41, 917, 1234, 1277, 1382, 1460
CviQI GTAC 2 cut(s) 367, 1274
DdeI CTNAG 7 cut(s) 359, 384, 500, 1014, 1331, 1374, 1579
DpnI GATC 6 cut(s) 18, 621, 717, 834, 929, 1605
DpnII GATC 6 cut(s) 16, 619, 715, 832, 927, 1603
Eam1104I CTCTTC 1 cut(s) 1013
EarI CTCTTC 1 cut(s) 1013
Ecl136II GAGCTC 1 cut(s) 1378
Eco105I TACGTA 1 cut(s) 1028
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 1380
Eco31I GGTCTC 3 cut(s) 271, 1129, 1650
Eco32I GATATC 1 cut(s) 1430
Eco47III AGCGCT 1 cut(s) 1577
Eco53kI GAGCTC 1 cut(s) 1378
Eco57I CTGAAG 2 cut(s) 896, 1638
Eco81I CCTNAGG 1 cut(s) 1331
Eco88I CYCGRG 1 cut(s) 631
Eco91I GGTNACC 1 cut(s) 1067
EcoICRI GAGCTC 1 cut(s) 1378
EcoO65I GGTNACC 1 cut(s) 1067
EcoRII CCWGG 3 cut(s) 135, 1048, 1342
EcoRV GATATC 1 cut(s) 1430
EcoT14I CCWWGG 1 cut(s) 12
EcoT22I ATGCAT 1 cut(s) 1510
EcoT38I GRGCYC 1 cut(s) 1380
ErhI CCWWGG 1 cut(s) 12
Esp3I CGTCTC 1 cut(s) 288
FaeI CATG 6 cut(s) 44, 920, 1237, 1280, 1385, 1463
FalI AAGNNNNNCTT 2 cut(s) 875, 907
FatI CATG 6 cut(s) 40, 916, 1233, 1276, 1381, 1459
FauNDI CATATG 1 cut(s) 838
FbaI TGATCA 2 cut(s) 619, 927
FokI GGATG 3 cut(s) 869, 1283, 1418
FriOI GRGCYC 1 cut(s) 1380
FspBI CTAG 3 cut(s) 459, 1178, 1421
GlaI GCGC 2 cut(s) 357, 1577
HaeII RGCGCY 1 cut(s) 1579
HaeIII GGCC 7 cut(s) 45, 662, 946, 962, 1232, 1395, 1445
HgaI GACGC 1 cut(s) 330
HhaI GCGC 2 cut(s) 358, 1578
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 6 cut(s) 44, 920, 1237, 1280, 1385, 1463
Hin6I GCGC 2 cut(s) 356, 1576
HinP1I GCGC 2 cut(s) 356, 1576
HindIII AAGCTT 1 cut(s) 221
HinfI GANTC 9 cut(s) 152, 466, 580, 589, 629, 634, 935, 1322, 1451
HphI GGTGA 2 cut(s) 81, 337
Hpy188I TCNGA 3 cut(s) 876, 889, 1355
Hpy188III TCNNGA 8 cut(s) 164, 252, 306, 459, 701, 733, 1127, 1607
Hpy99I CGWCG 2 cut(s) 253, 1037
HpyAV CCTTC 6 cut(s) 1057, 1222, 1243, 1297, 1385, 1588
HpyCH4IV ACGT 3 cut(s) 6, 727, 1027
HpyF3I CTNAG 7 cut(s) 359, 384, 500, 1014, 1331, 1374, 1579
HpySE526I ACGT 3 cut(s) 6, 727, 1027
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 6 cut(s) 44, 920, 1237, 1280, 1385, 1463
HspAI GCGC 2 cut(s) 356, 1576
Ksp22I TGATCA 2 cut(s) 619, 927
Kzo9I GATC 6 cut(s) 16, 619, 715, 832, 927, 1603
LguI GCTCTTC 1 cut(s) 1013
LmnI GCTCC 5 cut(s) 37, 371, 998, 1355, 1383
LweI GCATC 5 cut(s) 620, 1396, 1495, 1600, 1636
MabI ACCWGGT 2 cut(s) 1048, 1342
MaeI CTAG 3 cut(s) 459, 1178, 1421
MaeII ACGT 3 cut(s) 6, 727, 1027
MaeIII GTNAC 5 cut(s) 109, 844, 1067, 1283, 1397
MalI GATC 6 cut(s) 18, 621, 717, 834, 929, 1605
MboI GATC 6 cut(s) 16, 619, 715, 832, 927, 1603
MboII GAAGA 8 cut(s) 78, 511, 799, 889, 1030, 1631, 1634, 1640
MflI RGATCY 2 cut(s) 715, 1603
MhlI GDGCHC 1 cut(s) 1380
MlyI GAGTC 6 cut(s) 583, 623, 643, 929, 1331, 1445
MmeI TCCRAC 1 cut(s) 266
Mph1103I ATGCAT 1 cut(s) 1510
MseI TTAA 4 cut(s) 297, 312, 827, 1194
MspA1I CMGCKG 2 cut(s) 95, 1103
MspR9I CCNGG 3 cut(s) 137, 1050, 1344
MvaI CCWGG 3 cut(s) 137, 1050, 1344
NdeI CATATG 1 cut(s) 838
NdeII GATC 6 cut(s) 16, 619, 715, 832, 927, 1603
NlaIII CATG 6 cut(s) 44, 920, 1237, 1280, 1385, 1463
NlaIV GGNNCC 2 cut(s) 1341, 1605
NmeAIII GCCGAG 1 cut(s) 35
NmuCI GTSAC 2 cut(s) 109, 844
NsiI ATGCAT 1 cut(s) 1510
NspI RCATGY 2 cut(s) 1280, 1463
PaeR7I CTCGAG 1 cut(s) 631
PciI ACATGT 1 cut(s) 1459
PciSI GCTCTTC 1 cut(s) 1013
PfeI GAWTC 3 cut(s) 152, 466, 580
PleI GAGTC 6 cut(s) 583, 623, 642, 929, 1330, 1445
PpsI GAGTC 6 cut(s) 583, 623, 642, 929, 1330, 1445
Ppu21I YACGTR 1 cut(s) 1028
PscI ACATGT 1 cut(s) 1459
PshAI GACNNNNGTC 1 cut(s) 633
PshBI ATTAAT 2 cut(s) 312, 827
Psp124BI GAGCTC 1 cut(s) 1380
Psp1406I AACGTT 1 cut(s) 727
Psp6I CCWGG 3 cut(s) 135, 1048, 1342
PspEI GGTNACC 1 cut(s) 1067
PspGI CCWGG 3 cut(s) 135, 1048, 1342
PspN4I GGNNCC 2 cut(s) 1341, 1605
PspPI GGNCC 2 cut(s) 661, 1443
PspXI VCTCGAGB 1 cut(s) 631
PsuI RGATCY 2 cut(s) 715, 1603
PvuII CAGCTG 2 cut(s) 95, 1103
RsaI GTAC 2 cut(s) 368, 1275
RsaNI GTAC 2 cut(s) 367, 1274
SacI GAGCTC 1 cut(s) 1380
SapI GCTCTTC 1 cut(s) 1013
SaqAI TTAA 4 cut(s) 297, 312, 827, 1194
Sau3AI GATC 6 cut(s) 16, 619, 715, 832, 927, 1603
Sau96I GGNCC 2 cut(s) 661, 1443
SchI GAGTC 6 cut(s) 583, 623, 643, 929, 1331, 1445
ScrFI CCNGG 3 cut(s) 137, 1050, 1344
SduI GDGCHC 1 cut(s) 1380
SexAI ACCWGGT 2 cut(s) 1048, 1342
SfaNI GCATC 5 cut(s) 620, 1396, 1495, 1600, 1636
Sfr274I CTCGAG 1 cut(s) 631
SlaI CTCGAG 1 cut(s) 631
SmlI CTYRAG 4 cut(s) 631, 1125, 1433, 1473
SmoI CTYRAG 4 cut(s) 631, 1125, 1433, 1473
SnaBI TACGTA 1 cut(s) 1028
SsiI CCGC 2 cut(s) 116, 584
SspI AATATT 1 cut(s) 397
SspMI CTAG 3 cut(s) 459, 1178, 1421
SstI GAGCTC 1 cut(s) 1380
StyD4I CCNGG 3 cut(s) 135, 1048, 1342
StyI CCWWGG 1 cut(s) 12
TaiI ACGT 3 cut(s) 9, 730, 1030
TaqI TCGA 6 cut(s) 251, 522, 632, 823, 1032, 1641
TaqII GACCGA 1 cut(s) 264
TatI WGTACW 1 cut(s) 1273
TfiI GAWTC 3 cut(s) 152, 466, 580
Tru1I TTAA 4 cut(s) 297, 312, 827, 1194
Tru9I TTAA 4 cut(s) 297, 312, 827, 1194
TscAI CASTG 2 cut(s) 671, 937
TseFI GTSAC 2 cut(s) 109, 844
Tsp45I GTSAC 2 cut(s) 109, 844
TspGWI ACGGA 1 cut(s) 384
TspRI CASTG 2 cut(s) 671, 937
VspI ATTAAT 2 cut(s) 312, 827
XapI RAATTY 2 cut(s) 427, 808
XbaI TCTAGA 1 cut(s) 458
XceI RCATGY 2 cut(s) 1280, 1463
XhoI CTCGAG 1 cut(s) 631
XspI CTAG 3 cut(s) 459, 1178, 1421
ZraI GACGTC 1 cut(s) 7
Zsp2I ATGCAT 1 cut(s) 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.