Rorug04G0171200

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009120
Physical Location & Seq
Reverse (-)
30148631 .. 30150428
1798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0171200.1

Sequence Viewer

Length: 822 bp
ATGCCTTGGATGACCCTCGGAGGGATCATCGCTCTCCCTCTGTTTATCTTCTTGAGGGTCAGAACACTAAACTGCCTCGATTATGATCACAAATTGGCACAGAAGACCATCAAATCGGTTCCTCCAATGATTCAGAGGTTCGGGTTTGCGAATAAGGAAGCAGTTGCTAACTCCTTCAGAGCTCAAGGATCGGTTTTCAGACATCCGTTTTGTTCGGTTACCCAATATAAATGGTTTACTTTACAAGTTGTATCCAAAATGATGGTTGAATTGGGTTGTGATGTTGGGCAACTCTTGGGGTTTTGTAGGATCTATCTGGTTGGGGAAACTCTTTCAGGATGTTGGGCAACTGTTGGAGTGTTGATTGAGAAGGGCACTAAAAGAGCTAGTTCTACAGGGAAGAGCTATTCGATATACAAATTCGAGTGTTTGGAGGAAGATACCGTTTCTGTTTTCTTGTTTGGTGATGCTTATGAGAGGAACTGCAATGAGCAGGCTGGAATGGTGTTTGCACTGTTTAATTGTAGCCTACGCAAGGATGCTAAGGGTGCTGGTTTTTCTTTGAGTGTGTTTTCGGCCAATCAAATTGTGGAGATAGGAACTTCAGTTGATTATGGAGTCTGCAGAGGGAAAAGGAAGGATGGAATGGCTTGTACTTTAGTCATAAATAAGTATGTAACATGCTTTTCTTCAATCACCTTATTTCAAAGTCATATAGTTAGAACTACAAGTGCTGTCATTATGTGCATCTCTATTTGTAGAGGTTTTTGTACCTTTTATTTATTAATATTTGTAACAATATTTCTCTCCTCTTATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166
Pfam Domains
Protein Families
TPR_16 TPR_19 TPR_7 TPR_8

Protein Analysis

273

Amino Acids

30.47

Weight (kDa)

8.98

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_MCM10 PF22379 105 - 197 6.7e-35 MCM10 OB-fold
zf-primase PF09329 200 - 226 2.1e-06 Primase zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 32, 196, 317
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 1 cut(s) 419
AcuI CTGAAG 2 cut(s) 160, 588
AfaI GTAC 2 cut(s) 655, 772
AfiI CCNNNNNNNGG 2 cut(s) 21, 535
AgsI TTSAA 3 cut(s) 269, 693, 707
AluBI AGCT 3 cut(s) 182, 386, 405
AluI AGCT 3 cut(s) 182, 386, 405
Alw21I GWGCWC 1 cut(s) 184
AlwI GGATC 3 cut(s) 32, 196, 317
AoxI GGCC 1 cut(s) 576
ApoI RAATTY 1 cut(s) 419
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
AseI ATTAAT 1 cut(s) 785
AsuHPI GGTGA 2 cut(s) 476, 688
BaeGI GKGCMC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 184
BccI CCATC 3 cut(s) 116, 256, 635
BciVI GTATCC 1 cut(s) 262
BclI TGATCA 1 cut(s) 85
BfaI CTAG 1 cut(s) 387
BfmI CTRYAG 2 cut(s) 393, 622
BfuI GTATCC 1 cut(s) 262
BmiI GGNNCC 1 cut(s) 120
BmsI GCATC 3 cut(s) 457, 529, 756
BpiI GAAGAC 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 543
BpuEI CTTGAG 2 cut(s) 73, 168
BsaBI GATNNNNATC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 5, 16
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 535
Bse3DI GCAATG 1 cut(s) 493
Bse8I GATNNNNATC 1 cut(s) 84
BseDI CCNNGG 2 cut(s) 5, 16
BseGI GGATG 5 cut(s) 15, 202, 344, 544, 646
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 2 cut(s) 21, 535
BseMI GCAATG 1 cut(s) 493
BseRI GAGGAG 1 cut(s) 799
BseSI GKGCMC 1 cut(s) 377
BshFI GGCC 1 cut(s) 578
BsiHKAI GWGCWC 1 cut(s) 184
BslI CCNNNNNNNGG 2 cut(s) 21, 535
BsnI GGCC 1 cut(s) 578
Bsp1286I GDGCHC 2 cut(s) 184, 377
Bsp143I GATC 4 cut(s) 24, 85, 188, 309
BspANI GGCC 1 cut(s) 578
BspLI GGNNCC 1 cut(s) 120
BspMAI CTGCAG 1 cut(s) 626
BspPI GGATC 3 cut(s) 32, 196, 317
BspQI GCTCTTC 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 493
BssECI CCNNGG 2 cut(s) 5, 16
BssMI GATC 4 cut(s) 24, 85, 188, 309
BssT1I CCWWGG 1 cut(s) 5
Bst4CI ACNGT 3 cut(s) 352, 445, 516
Bst6I CTCTTC 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 495
BstDEI CTNAG 1 cut(s) 543
BstEII GGTNACC 1 cut(s) 217
BstENI CCTNNNNNAGG 1 cut(s) 533
BstF5I GGATG 5 cut(s) 15, 202, 344, 544, 646
BstKTI GATC 4 cut(s) 27, 88, 191, 312
BstMBI GATC 4 cut(s) 24, 85, 188, 309
BstMWI GCNNNNNNNGC 1 cut(s) 548
BstNSI RCATGY 1 cut(s) 684
BstPI GGTNACC 1 cut(s) 217
BstSFI CTRYAG 2 cut(s) 393, 622
BstSLI GKGCMC 1 cut(s) 377
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 309
BstXI CCANNNNNNTGG 1 cut(s) 262
BstYI RGATCY 1 cut(s) 309
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 1 cut(s) 578
BtgZI GCGATG 1 cut(s) 13
BtsCI GGATG 5 cut(s) 15, 202, 344, 544, 646
BtsIMutI CAGTG 1 cut(s) 512
Cac8I GCNNGC 1 cut(s) 495
Csp6I GTAC 2 cut(s) 654, 771
CviAII CATG 1 cut(s) 681
CviJI RGCY 7 cut(s) 182, 386, 405, 497, 528, 578, 650
CviKI_1 RGCY 7 cut(s) 182, 386, 405, 497, 528, 578, 650
CviQI GTAC 2 cut(s) 654, 771
DdeI CTNAG 1 cut(s) 543
DpnI GATC 4 cut(s) 26, 87, 190, 311
DpnII GATC 4 cut(s) 24, 85, 188, 309
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 1 cut(s) 395
EarI CTCTTC 1 cut(s) 395
Ecl136II GAGCTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 5
Eco24I GRGCYC 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 182
Eco57I CTGAAG 2 cut(s) 160, 588
Eco91I GGTNACC 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 182
EcoNI CCTNNNNNAGG 1 cut(s) 533
EcoO65I GGTNACC 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 5
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 5
FaeI CATG 1 cut(s) 684
FatI CATG 1 cut(s) 680
FbaI TGATCA 1 cut(s) 85
FokI GGATG 5 cut(s) 22, 189, 351, 551, 653
FriOI GRGCYC 1 cut(s) 184
FspBI CTAG 1 cut(s) 387
HaeIII GGCC 1 cut(s) 578
Hin1II CATG 1 cut(s) 684
HinfI GANTC 2 cut(s) 130, 618
HphI GGTGA 2 cut(s) 476, 688
Hpy166II GTNNAC 1 cut(s) 237
Hpy188I TCNGA 5 cut(s) 20, 62, 135, 179, 200
Hpy188III TCNNGA 2 cut(s) 52, 336
Hpy8I GTNNAC 1 cut(s) 237
HpyAV CCTTC 3 cut(s) 184, 364, 631
HpyCH4III ACNGT 3 cut(s) 352, 445, 516
HpyCH4V TGCA 4 cut(s) 486, 512, 624, 747
HpyF10VI GCNNNNNNNGC 1 cut(s) 548
HpyF3I CTNAG 1 cut(s) 543
Hsp92II CATG 1 cut(s) 684
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 4 cut(s) 24, 85, 188, 309
LguI GCTCTTC 1 cut(s) 395
LpnPI CCDG 6 cut(s) 302, 321, 381, 479, 483, 537
LweI GCATC 3 cut(s) 457, 529, 756
MaeI CTAG 1 cut(s) 387
MaeIII GTNAC 3 cut(s) 217, 676, 793
MalI GATC 4 cut(s) 26, 87, 190, 311
MboI GATC 4 cut(s) 24, 85, 188, 309
MboII GAAGA 5 cut(s) 40, 115, 412, 449, 681
MflI RGATCY 1 cut(s) 309
MhlI GDGCHC 2 cut(s) 184, 377
MluCI AATT 5 cut(s) 92, 269, 419, 520, 585
MlyI GAGTC 1 cut(s) 627
MmeI TCCRAC 1 cut(s) 334
MseI TTAA 2 cut(s) 519, 785
MwoI GCNNNNNNNGC 1 cut(s) 548
NdeII GATC 4 cut(s) 24, 85, 188, 309
NlaIII CATG 1 cut(s) 684
NlaIV GGNNCC 1 cut(s) 120
NspI RCATGY 1 cut(s) 684
PciSI GCTCTTC 1 cut(s) 395
PfeI GAWTC 1 cut(s) 130
PleI GAGTC 1 cut(s) 626
PpsI GAGTC 1 cut(s) 626
PshBI ATTAAT 1 cut(s) 785
Psp124BI GAGCTC 1 cut(s) 184
PspEI GGTNACC 1 cut(s) 217
PspN4I GGNNCC 1 cut(s) 120
PstI CTGCAG 1 cut(s) 626
PsuI RGATCY 1 cut(s) 309
RsaI GTAC 2 cut(s) 655, 772
RsaNI GTAC 2 cut(s) 654, 771
SacI GAGCTC 1 cut(s) 184
SapI GCTCTTC 1 cut(s) 395
SaqAI TTAA 2 cut(s) 519, 785
Sau3AI GATC 4 cut(s) 24, 85, 188, 309
SchI GAGTC 1 cut(s) 627
SduI GDGCHC 2 cut(s) 184, 377
SetI ASST 7 cut(s) 140, 184, 388, 407, 701, 766, 776
SfaNI GCATC 3 cut(s) 457, 529, 756
SfcI CTRYAG 2 cut(s) 393, 622
SmlI CTYRAG 2 cut(s) 52, 183
SmoI CTYRAG 2 cut(s) 52, 183
Sse9I AATT 5 cut(s) 92, 269, 419, 520, 585
SspI AATATT 2 cut(s) 789, 801
SspMI CTAG 1 cut(s) 387
SstI GAGCTC 1 cut(s) 184
StyI CCWWGG 1 cut(s) 5
TaaI ACNGT 3 cut(s) 352, 445, 516
TaqI TCGA 3 cut(s) 78, 410, 423
TasI AATT 5 cut(s) 92, 269, 419, 520, 585
TatI WGTACW 1 cut(s) 653
TfiI GAWTC 1 cut(s) 130
Tru1I TTAA 2 cut(s) 519, 785
Tru9I TTAA 2 cut(s) 519, 785
TscAI CASTG 1 cut(s) 519
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 1 cut(s) 519
VspI ATTAAT 1 cut(s) 785
XagI CCTNNNNNAGG 1 cut(s) 533
XapI RAATTY 1 cut(s) 419
XceI RCATGY 1 cut(s) 684
XcmI CCANNNNNNNNNTGG 1 cut(s) 586
XspI CTAG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.