RchiOBHm_Chr5g0014851

threonine-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0021435
Physical Location & Seq
Forward (+)
10248262 .. 10250414
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29528

Sequence Viewer

Length: 1119 bp
ATGTCAAAATCCTCTTCGTCTCGTCGTTCACGTGGCAGTCGCAGCTCACTTCTAGATAAGCAAGTGACAGGGACAACAACCTTGACTCTTATTGGTGAAGAAGTGATATATGTGGTCACTGACGAGAGGTCTAGTACCGAGCCACCTCCTTACATGATATTGAACGATAATGCCTCAAAAACTTACGCTATTTCAACAAATGTGTTGGCTACAATAGCTGGAAATCCGGATCTTTGTTCGACCATGCTGGCACATGTAAAAGCGACAGTCAGTCAAGAATTAGTTTCTGATCAACTTGATAGCAACAATGTTGTGCATATTGCAGCAAATTATGTCCATGAATGTGTCACTGTATGGGAGCGTGATAACCCAAACATGCACTTCCCGTCTGCAACACAAATAGTTGGTTGGTGCAATGGTGAACCTTCAGTATATGGCATTCAATGTGCTAACCCACCACAGGTGACACATGAGCACGGAAGTGGATTTGTCCATGGATCCGGTGAGAAATCTGTTCAAGATTACTATAAGAAAGAGGACCCAATGCCATACAAGCGCGGCCGTCCACGTAGATCAGATATTGAACTTGCCCAAATTGGAAAAAGAGCAGTAGTGTTTTCAGCGTTGAATGAGCGTTATACTGGTGGAAGAGTGTGTTCAGTGGAGGTGAGACCAGATATGATTCATGAATCAGGTTCAGAAGAGATTCTCAAGGTACTACATGATGATTATGACTACTTTAAAACCTACTTATCACATTTTCTTTTCTGTCTTTACCGCAAGAGTGATTTTGAATATACGATCGACACTGAGGCAATGTTGAGTCAAGTAATCCACACTCGATTCACTTCTGGGATTCAAAGGTCTCATGTTATTCAAATCAAAGAAAAATATTTTATCCGCATCTTACAATTTCCGGATGGAGAAGTTGCTAAAGAAGAATTTCAGGCTGTAAAGTCAGAGTGGAAACAACAGCTGGGCTACGGGCATACCCAAGATGTTGATGTGCATCCCCATATTCGTTCATTGCCGTGGATCGAGCTTCATCAATTGCAGGGTGCTCCACTTGTCTTTGCTATGGCCACAAAAAAGTATATTAGTCTTTTTCTTGATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 ko03050 map03008 map03050
Pfam Domains
Protein Families
No domains found.

Protein Analysis

372

Amino Acids

42.11

Weight (kDa)

6.15

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 23 - 216 9.3e-07 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021435)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0014851
rosa_laevigata RLG00000032148
rosa_roxburghii Rroxscaffold_1G00061720
rosa_samantha Rh5BG113600 Rh5CG125300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 558
AccIII TCCGGA 2 cut(s) 226, 916
AciI CCGC 3 cut(s) 558, 778, 901
AclWI GGATC 4 cut(s) 237, 492, 505, 1043
AcoI YGGCCR 2 cut(s) 559, 1080
AcsI RAATTY 1 cut(s) 941
AcuI CTGAAG 1 cut(s) 411
AcvI CACGTG 1 cut(s) 32
AfaI GTAC 2 cut(s) 136, 717
AfiI CCNNNNNNNGG 1 cut(s) 460
AflIII ACRYGT 1 cut(s) 253
AgsI TTSAA 9 cut(s) 163, 195, 443, 518, 584, 628, 794, 860, 878
AhdI GACNNNNNGTC 2 cut(s) 127, 270
AleI CACNNNNGTG 1 cut(s) 480
AluBI AGCT 4 cut(s) 45, 218, 976, 1042
AluI AGCT 4 cut(s) 45, 218, 976, 1042
Alw21I GWGCWC 2 cut(s) 477, 1063
Alw26I GTCTC 3 cut(s) 24, 664, 870
AlwI GGATC 4 cut(s) 237, 492, 505, 1043
Aor13HI TCCGGA 2 cut(s) 226, 916
AoxI GGCC 2 cut(s) 559, 1080
ApeKI GCWGC 2 cut(s) 42, 323
ApoI RAATTY 1 cut(s) 941
Asp700I GAANNNNTTC 2 cut(s) 705, 942
AspLEI GCGC 1 cut(s) 558
AspS9I GGNCC 1 cut(s) 538
AsuHPI GGTGA 5 cut(s) 107, 431, 475, 515, 679
AvaII GGWCC 1 cut(s) 538
BalI TGGCCA 1 cut(s) 1082
BamHI GGATCC 1 cut(s) 497
BbrPI CACGTG 1 cut(s) 32
Bbv12I GWGCWC 2 cut(s) 477, 1063
BbvI GCAGC 2 cut(s) 54, 335
BccI CCATC 1 cut(s) 914
BceAI ACGGC 2 cut(s) 546, 1015
BclI TGATCA 1 cut(s) 289
BcoDI GTCTC 3 cut(s) 24, 664, 870
BfaI CTAG 2 cut(s) 53, 132
BisI GCNGC 3 cut(s) 43, 324, 559
BlsI GCNGC 3 cut(s) 44, 325, 560
Bme18I GGWCC 1 cut(s) 538
BmeRI GACNNNNNGTC 2 cut(s) 127, 270
BmgT120I GGNCC 1 cut(s) 538
BmiI GGNNCC 2 cut(s) 499, 540
BmsI GCATC 2 cut(s) 912, 1018
BpuEI CTTGAG 1 cut(s) 695
BsaAI YACGTR 2 cut(s) 32, 569
BsaBI GATNNNNATC 1 cut(s) 1008
BsaI GGTCTC 2 cut(s) 664, 870
BsaJI CCNNGG 2 cut(s) 493, 1031
BsaWI WCCGGW 3 cut(s) 226, 500, 916
Bsc4I CCNNNNNNNGG 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 646
Bse3DI GCAATG 3 cut(s) 421, 822, 1025
Bse8I GATNNNNATC 1 cut(s) 1008
BseAI TCCGGA 2 cut(s) 226, 916
BseDI CCNNGG 2 cut(s) 493, 1031
BseGI GGATG 2 cut(s) 925, 1009
BseJI GATNNNNATC 1 cut(s) 1008
BseLI CCNNNNNNNGG 1 cut(s) 460
BseMI GCAATG 3 cut(s) 421, 822, 1025
BseMII CTCAG 1 cut(s) 801
BseNI ACTGG 1 cut(s) 646
BseX3I CGGCCG 1 cut(s) 559
BseXI GCAGC 2 cut(s) 54, 335
BseYI CCCAGC 1 cut(s) 976
Bsh1236I CGCG 1 cut(s) 558
Bsh1285I CGRYCG 2 cut(s) 562, 804
BshFI GGCC 2 cut(s) 561, 1082
BsiEI CGRYCG 2 cut(s) 562, 804
BsiHKAI GWGCWC 2 cut(s) 477, 1063
BsiSI CCGG 3 cut(s) 227, 501, 917
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 1 cut(s) 460
BsmAI GTCTC 3 cut(s) 24, 664, 870
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 85
BsmI GAATGC 1 cut(s) 438
BsnI GGCC 2 cut(s) 561, 1082
Bso31I GGTCTC 2 cut(s) 664, 870
Bsp1286I GDGCHC 2 cut(s) 477, 1063
Bsp13I TCCGGA 2 cut(s) 226, 916
Bsp143I GATC 7 cut(s) 229, 289, 497, 572, 801, 1035, 1111
Bsp19I CCATGG 1 cut(s) 493
BspACI CCGC 3 cut(s) 558, 778, 901
BspANI GGCC 2 cut(s) 561, 1082
BspCNI CTCAG 1 cut(s) 802
BspEI TCCGGA 2 cut(s) 226, 916
BspFNI CGCG 1 cut(s) 558
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 2 cut(s) 499, 540
BspPI GGATC 4 cut(s) 237, 492, 505, 1043
BspTNI GGTCTC 2 cut(s) 664, 870
BsrDI GCAATG 3 cut(s) 421, 822, 1025
BsrI ACTGG 1 cut(s) 646
BssECI CCNNGG 2 cut(s) 493, 1031
BssMI GATC 7 cut(s) 229, 289, 497, 572, 801, 1035, 1111
BssT1I CCWWGG 1 cut(s) 493
Bst4CI ACNGT 2 cut(s) 268, 352
Bst6I CTCTTC 3 cut(s) 19, 643, 696
BstBAI YACGTR 2 cut(s) 32, 569
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 1 cut(s) 810
BstDSI CCRYGG 2 cut(s) 493, 1031
BstF5I GGATG 2 cut(s) 925, 1009
BstFNI CGCG 1 cut(s) 558
BstHHI GCGC 1 cut(s) 558
BstKTI GATC 7 cut(s) 232, 292, 500, 575, 804, 1038, 1114
BstMAI GTCTC 3 cut(s) 24, 664, 870
BstMBI GATC 7 cut(s) 229, 289, 497, 572, 801, 1035, 1111
BstMCI CGRYCG 2 cut(s) 562, 804
BstMWI GCNNNNNNNGC 3 cut(s) 42, 215, 553
BstNSI RCATGY 2 cut(s) 257, 379
BstUI CGCG 1 cut(s) 558
BstV1I GCAGC 2 cut(s) 54, 335
BstX2I RGATCY 2 cut(s) 229, 497
BstYI RGATCY 2 cut(s) 229, 497
BstZI CGGCCG 1 cut(s) 559
BsuRI GGCC 2 cut(s) 561, 1082
BtgI CCRYGG 2 cut(s) 493, 1031
BtsCI GGATG 2 cut(s) 925, 1009
BtsIMutI CAGTG 4 cut(s) 117, 348, 666, 807
Cac8I GCNNGC 1 cut(s) 249
CciI TCATGA 1 cut(s) 685
CfoI GCGC 1 cut(s) 558
Cfr13I GGNCC 1 cut(s) 538
Csp6I GTAC 2 cut(s) 135, 716
CviQI GTAC 2 cut(s) 135, 716
DdeI CTNAG 1 cut(s) 810
DpnI GATC 7 cut(s) 231, 291, 499, 574, 803, 1037, 1113
DpnII GATC 7 cut(s) 229, 289, 497, 572, 801, 1035, 1111
DraI TTTAAA 1 cut(s) 742
DriI GACNNNNNGTC 2 cut(s) 127, 270
EaeI YGGCCR 2 cut(s) 559, 1080
EagI CGGCCG 1 cut(s) 559
Eam1104I CTCTTC 3 cut(s) 19, 643, 696
Eam1105I GACNNNNNGTC 2 cut(s) 127, 270
EarI CTCTTC 3 cut(s) 19, 643, 696
EclXI CGGCCG 1 cut(s) 559
Eco130I CCWWGG 1 cut(s) 493
Eco31I GGTCTC 2 cut(s) 664, 870
Eco47I GGWCC 1 cut(s) 538
Eco52I CGGCCG 1 cut(s) 559
Eco57I CTGAAG 1 cut(s) 411
Eco72I CACGTG 1 cut(s) 32
EcoO109I RGGNCCY 1 cut(s) 538
EcoT14I CCWWGG 1 cut(s) 493
ErhI CCWWGG 1 cut(s) 493
Esp3I CGTCTC 1 cut(s) 24
FaqI GGGAC 1 cut(s) 85
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 3 cut(s) 43, 324, 559
FokI GGATG 2 cut(s) 932, 996
Fsp4HI GCNGC 3 cut(s) 43, 324, 559
FspBI CTAG 2 cut(s) 53, 132
GlaI GCGC 1 cut(s) 557
GluI GCNGC 3 cut(s) 43, 324, 559
GsaI CCCAGC 1 cut(s) 980
HaeIII GGCC 2 cut(s) 561, 1082
HapII CCGG 3 cut(s) 227, 501, 917
HhaI GCGC 1 cut(s) 558
Hin6I GCGC 1 cut(s) 556
HinP1I GCGC 1 cut(s) 556
HinfI GANTC 7 cut(s) 85, 682, 689, 706, 823, 843, 856
HpaII CCGG 3 cut(s) 227, 501, 917
HphI GGTGA 5 cut(s) 107, 431, 475, 515, 679
Hpy166II GTNNAC 3 cut(s) 29, 422, 566
Hpy188I TCNGA 4 cut(s) 289, 577, 700, 961
Hpy188III TCNNGA 7 cut(s) 53, 227, 275, 518, 686, 917, 1109
Hpy8I GTNNAC 3 cut(s) 29, 422, 566
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 1 cut(s) 435
HpyCH4III ACNGT 2 cut(s) 268, 352
HpyCH4IV ACGT 2 cut(s) 31, 568
HpyCH4V TGCA 7 cut(s) 316, 323, 379, 392, 414, 1009, 1054
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 215, 553
HpyF3I CTNAG 1 cut(s) 810
HpySE526I ACGT 2 cut(s) 31, 568
HspAI GCGC 1 cut(s) 556
Kpn2I TCCGGA 2 cut(s) 226, 916
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 7 cut(s) 229, 289, 497, 572, 801, 1035, 1111
LmnI GCTCC 2 cut(s) 358, 1066
Lsp1109I GCAGC 2 cut(s) 54, 335
LweI GCATC 2 cut(s) 912, 1018
MaeI CTAG 2 cut(s) 53, 132
MaeII ACGT 2 cut(s) 31, 568
MaeIII GTNAC 4 cut(s) 64, 115, 346, 463
MalI GATC 7 cut(s) 231, 291, 499, 574, 803, 1037, 1113
MboI GATC 7 cut(s) 229, 289, 497, 572, 801, 1035, 1111
MboII GAAGA 5 cut(s) 6, 110, 660, 713, 950
MfeI CAATTG 1 cut(s) 1049
MflI RGATCY 2 cut(s) 229, 497
MhlI GDGCHC 2 cut(s) 477, 1063
MlsI TGGCCA 1 cut(s) 1082
MluCI AATT 6 cut(s) 278, 328, 594, 911, 941, 1049
MluNI TGGCCA 1 cut(s) 1082
MlyI GAGTC 2 cut(s) 79, 832
MnlI CCTC 7 cut(s) 22, 120, 156, 184, 529, 658, 805
Mox20I TGGCCA 1 cut(s) 1082
MroI TCCGGA 2 cut(s) 226, 916
MroXI GAANNNNTTC 2 cut(s) 705, 942
MscI TGGCCA 1 cut(s) 1082
MseI TTAA 2 cut(s) 741, 1117
MslI CAYNNNNRTG 3 cut(s) 342, 480, 1030
Msp20I TGGCCA 1 cut(s) 1082
MspA1I CMGCKG 1 cut(s) 976
MspI CCGG 3 cut(s) 227, 501, 917
MunI CAATTG 1 cut(s) 1049
Mva1269I GAATGC 1 cut(s) 438
MvnI CGCG 1 cut(s) 558
MwoI GCNNNNNNNGC 3 cut(s) 42, 215, 553
NcoI CCATGG 1 cut(s) 493
NdeII GATC 7 cut(s) 229, 289, 497, 572, 801, 1035, 1111
NlaIV GGNNCC 2 cut(s) 499, 540
NmuCI GTSAC 4 cut(s) 64, 115, 346, 463
NspI RCATGY 2 cut(s) 257, 379
OliI CACNNNNGTG 1 cut(s) 480
PagI TCATGA 1 cut(s) 685
PciI ACATGT 1 cut(s) 253
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PctI GAATGC 1 cut(s) 438
PdmI GAANNNNTTC 2 cut(s) 705, 942
PfeI GAWTC 5 cut(s) 682, 689, 706, 843, 856
PkrI GCNGC 3 cut(s) 44, 325, 560
Ple19I CGATCG 1 cut(s) 804
PleI GAGTC 2 cut(s) 79, 831
PmaCI CACGTG 1 cut(s) 32
PmlI CACGTG 1 cut(s) 32
PpsI GAGTC 2 cut(s) 79, 831
Ppu21I YACGTR 2 cut(s) 32, 569
PpuMI RGGWCCY 1 cut(s) 538
PscI ACATGT 1 cut(s) 253
Psp5II RGGWCCY 1 cut(s) 538
PspCI CACGTG 1 cut(s) 32
PspFI CCCAGC 1 cut(s) 976
PspN4I GGNNCC 2 cut(s) 499, 540
PspPI GGNCC 1 cut(s) 538
PspPPI RGGWCCY 1 cut(s) 538
PsuI RGATCY 2 cut(s) 229, 497
PvuI CGATCG 1 cut(s) 804
PvuII CAGCTG 1 cut(s) 976
RsaI GTAC 2 cut(s) 136, 717
RsaNI GTAC 2 cut(s) 135, 716
RseI CAYNNNNRTG 3 cut(s) 342, 480, 1030
SaqAI TTAA 2 cut(s) 741, 1117
SatI GCNGC 3 cut(s) 43, 324, 559
Sau3AI GATC 7 cut(s) 229, 289, 497, 572, 801, 1035, 1111
Sau96I GGNCC 1 cut(s) 538
SchI GAGTC 2 cut(s) 79, 832
SduI GDGCHC 2 cut(s) 477, 1063
SfaNI GCATC 2 cut(s) 912, 1018
SinI GGWCC 1 cut(s) 538
SmiMI CAYNNNNRTG 3 cut(s) 342, 480, 1030
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
Sse9I AATT 6 cut(s) 278, 328, 594, 911, 941, 1049
SsiI CCGC 3 cut(s) 558, 778, 901
SspI AATATT 1 cut(s) 893
SspMI CTAG 2 cut(s) 53, 132
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 2 cut(s) 268, 352
TaiI ACGT 2 cut(s) 34, 571
TaqI TCGA 4 cut(s) 239, 804, 841, 1038
TasI AATT 6 cut(s) 278, 328, 594, 911, 941, 1049
TauI GCSGC 1 cut(s) 561
TfiI GAWTC 5 cut(s) 682, 689, 706, 843, 856
Tru1I TTAA 2 cut(s) 741, 1117
Tru9I TTAA 2 cut(s) 741, 1117
TscAI CASTG 4 cut(s) 124, 355, 666, 814
TseFI GTSAC 4 cut(s) 64, 115, 346, 463
TseI GCWGC 2 cut(s) 42, 323
Tsp45I GTSAC 4 cut(s) 64, 115, 346, 463
TspDTI ATGAA 5 cut(s) 354, 674, 702, 1014, 1034
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 4 cut(s) 124, 355, 666, 814
VpaK11BI GGWCC 1 cut(s) 538
XapI RAATTY 1 cut(s) 941
XbaI TCTAGA 1 cut(s) 52
XceI RCATGY 2 cut(s) 257, 379
XmnI GAANNNNTTC 2 cut(s) 705, 942
XspI CTAG 2 cut(s) 53, 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.