RLG00000032148

threonine-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0021435
Physical Location & Seq
Forward (+)
11410655 .. 11412157
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032148

Sequence Viewer

Length: 1152 bp
ATGAATTCATCTAGGTTAACACTACGTCAAAACCCTCTTCGTCTCGTCGTTACGTGGCAGTCGCAGCTCACTTCTAGGTATTCTCTTGATAAGCAAGTGACAGGGACAACAACCTTGACTCTTATTGGTGAAGAAGTGATATATGTGGTCACTGACGAGAGATCTAGTAGCCAGCCACCTCCTTACATGATATTGAACGATAATGTCTCAAAAACTTTCGTTATTTCAACAAATGTGTTAGCTACAATAGCTGGAAATCCGGATCTTTGTTCGACCATGCTGGCACATGTAAAAGCGACAGTCAGTCAAGAATTAGCTTATGATCAACTTGATAGCAGCAATGTTGTGCATATTGCAGCAAATTATGTCCATGAATGTGTCACTGTATGGGAGCGTGATAACCCAAACATGCACTTCCCGTCTGCAACACAAATAGTTGGTTGGTGCAACGGTGAACCTTCAGTATATGACATTCAATGTGTTGACCCACCACAGGTGACACATGAGCACGGAAGCGGATTTGTCCATGGATCCGGTGAGAAATCTGTTCAAGATTACTATAAGAAAGAGGACCCAATGCCATACAAGCGTGGCCGTCCACGTAGATCAGATATTGAACTTGCCCAAATTGGAAAAAGAGCAGTAGTGTTTTCAGCGTTGAATGAGCATTATACTGGTGGAAGAGTGTGTTCAGTGGAGGTGAGACCAGATATGATTCATAAATCAGATTCAGAAGAGATTCTCAAGGTACTACATGATGATTATGACTACTTTAAAACCTACTTATCACATTTTCTTTTCTGTCTTTACCGCAAAAGTGATTTTGAATATACGATCGACACTGAGGCAATGTTGAGTCAAGTAATCCACACTCGATTCACTTCTGGGATTCAAAGGTCTCATGTTATTCAAATCAAAGAAAAATATTTTATCCGCATCTTACAATTTCCGGATGGAGAAGTTGCTAAAGAAGAATTTCAGGCTGTAAAGTCAGAGTGGAAACAACAGCTGGGCTGCGGGCATACCCAAGATGTTGATGTGCATCCCCATATTCGTTCATTGCCGTGGATCGAGCTTCATCAATTGCAGGGTGCTCCACTTGTCTTTGCTATGGCCACAAAAAAGTATATTAGTCTTTTTCTTGATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 ko03050 map03008 map03050
Pfam Domains
Protein Families
No domains found.

Protein Analysis

384

Amino Acids

43.7

Weight (kDa)

6.01

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 34 - 227 1.1e-07 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021435)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0014851
rosa_laevigata RLG00000032148
rosa_roxburghii Rroxscaffold_1G00061720
rosa_samantha Rh5BG113600 Rh5CG125300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 259, 949
AciI CCGC 4 cut(s) 516, 811, 934, 1017
AclWI GGATC 4 cut(s) 270, 525, 538, 1076
AcoI YGGCCR 2 cut(s) 592, 1113
AcsI RAATTY 2 cut(s) 4, 974
AcuI CTGAAG 1 cut(s) 444
AfaI GTAC 1 cut(s) 750
AfiI CCNNNNNNNGG 1 cut(s) 493
AflIII ACRYGT 1 cut(s) 286
AgsI TTSAA 9 cut(s) 196, 228, 476, 551, 617, 661, 827, 893, 911
AhdI GACNNNNNGTC 1 cut(s) 303
AluBI AGCT 6 cut(s) 67, 242, 251, 317, 1009, 1075
AluI AGCT 6 cut(s) 67, 242, 251, 317, 1009, 1075
Alw21I GWGCWC 2 cut(s) 510, 1096
Alw26I GTCTC 4 cut(s) 47, 211, 697, 903
AlwI GGATC 4 cut(s) 270, 525, 538, 1076
Aor13HI TCCGGA 2 cut(s) 259, 949
AoxI GGCC 2 cut(s) 592, 1113
ApeKI GCWGC 4 cut(s) 64, 336, 356, 1014
ApoI RAATTY 2 cut(s) 4, 974
Asp700I GAANNNNTTC 2 cut(s) 738, 975
AspS9I GGNCC 1 cut(s) 571
AsuHPI GGTGA 5 cut(s) 140, 464, 508, 548, 712
AvaII GGWCC 1 cut(s) 571
BalI TGGCCA 1 cut(s) 1115
BamHI GGATCC 1 cut(s) 530
Bbv12I GWGCWC 2 cut(s) 510, 1096
BbvI GCAGC 4 cut(s) 76, 348, 368, 1001
BccI CCATC 1 cut(s) 947
BceAI ACGGC 2 cut(s) 579, 1048
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 4 cut(s) 47, 211, 697, 903
BfaI CTAG 3 cut(s) 12, 75, 165
BglII AGATCT 1 cut(s) 161
BisI GCNGC 4 cut(s) 65, 337, 357, 1015
BlsI GCNGC 4 cut(s) 66, 338, 358, 1016
Bme18I GGWCC 1 cut(s) 571
BmeRI GACNNNNNGTC 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 571
BmiI GGNNCC 2 cut(s) 532, 573
BmsI GCATC 2 cut(s) 945, 1051
BpuEI CTTGAG 1 cut(s) 728
BsaAI YACGTR 2 cut(s) 54, 602
BsaBI GATNNNNATC 1 cut(s) 1041
BsaI GGTCTC 2 cut(s) 697, 903
BsaJI CCNNGG 2 cut(s) 526, 1064
BsaWI WCCGGW 3 cut(s) 259, 533, 949
Bsc4I CCNNNNNNNGG 1 cut(s) 493
Bse1I ACTGG 1 cut(s) 679
Bse3DI GCAATG 3 cut(s) 346, 855, 1058
Bse8I GATNNNNATC 1 cut(s) 1041
BseAI TCCGGA 2 cut(s) 259, 949
BseDI CCNNGG 2 cut(s) 526, 1064
BseGI GGATG 2 cut(s) 958, 1042
BseJI GATNNNNATC 1 cut(s) 1041
BseLI CCNNNNNNNGG 1 cut(s) 493
BseMI GCAATG 3 cut(s) 346, 855, 1058
BseMII CTCAG 1 cut(s) 834
BseNI ACTGG 1 cut(s) 679
BseXI GCAGC 4 cut(s) 76, 348, 368, 1001
BseYI CCCAGC 1 cut(s) 1009
Bsh1285I CGRYCG 1 cut(s) 837
BshFI GGCC 2 cut(s) 594, 1115
BsiEI CGRYCG 1 cut(s) 837
BsiHKAI GWGCWC 2 cut(s) 510, 1096
BsiSI CCGG 3 cut(s) 260, 534, 950
BslFI GGGAC 1 cut(s) 118
BslI CCNNNNNNNGG 1 cut(s) 493
BsmAI GTCTC 4 cut(s) 47, 211, 697, 903
BsmBI CGTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 118
BsnI GGCC 2 cut(s) 594, 1115
Bso31I GGTCTC 2 cut(s) 697, 903
Bsp1286I GDGCHC 2 cut(s) 510, 1096
Bsp13I TCCGGA 2 cut(s) 259, 949
Bsp143I GATC 8 cut(s) 161, 262, 322, 530, 605, 834, 1068, 1144
Bsp19I CCATGG 1 cut(s) 526
BspACI CCGC 4 cut(s) 516, 811, 934, 1017
BspANI GGCC 2 cut(s) 594, 1115
BspCNI CTCAG 1 cut(s) 835
BspEI TCCGGA 2 cut(s) 259, 949
BspLI GGNNCC 2 cut(s) 532, 573
BspPI GGATC 4 cut(s) 270, 525, 538, 1076
BspTNI GGTCTC 2 cut(s) 697, 903
BsrDI GCAATG 3 cut(s) 346, 855, 1058
BsrI ACTGG 1 cut(s) 679
BssECI CCNNGG 2 cut(s) 526, 1064
BssMI GATC 8 cut(s) 161, 262, 322, 530, 605, 834, 1068, 1144
BssT1I CCWWGG 1 cut(s) 526
Bst4CI ACNGT 3 cut(s) 301, 385, 452
Bst6I CTCTTC 3 cut(s) 42, 676, 729
BstBAI YACGTR 2 cut(s) 54, 602
BstC8I GCNNGC 3 cut(s) 173, 282, 1019
BstDEI CTNAG 1 cut(s) 843
BstDSI CCRYGG 2 cut(s) 526, 1064
BstF5I GGATG 2 cut(s) 958, 1042
BstKTI GATC 8 cut(s) 164, 265, 325, 533, 608, 837, 1071, 1147
BstMAI GTCTC 4 cut(s) 47, 211, 697, 903
BstMBI GATC 8 cut(s) 161, 262, 322, 530, 605, 834, 1068, 1144
BstMCI CGRYCG 1 cut(s) 837
BstMWI GCNNNNNNNGC 3 cut(s) 64, 248, 586
BstNSI RCATGY 2 cut(s) 290, 412
BstV1I GCAGC 4 cut(s) 76, 348, 368, 1001
BstX2I RGATCY 3 cut(s) 161, 262, 530
BstYI RGATCY 3 cut(s) 161, 262, 530
BsuRI GGCC 2 cut(s) 594, 1115
BtgI CCRYGG 2 cut(s) 526, 1064
BtsCI GGATG 2 cut(s) 958, 1042
BtsIMutI CAGTG 4 cut(s) 150, 381, 699, 840
Cac8I GCNNGC 3 cut(s) 173, 282, 1019
Cfr13I GGNCC 1 cut(s) 571
Csp6I GTAC 1 cut(s) 749
CviAII CATG 9 cut(s) 187, 277, 287, 371, 409, 503, 527, 755, 902
CviQI GTAC 1 cut(s) 749
DdeI CTNAG 1 cut(s) 843
DpnI GATC 8 cut(s) 163, 264, 324, 532, 607, 836, 1070, 1146
DpnII GATC 8 cut(s) 161, 262, 322, 530, 605, 834, 1068, 1144
DraI TTTAAA 1 cut(s) 775
DriI GACNNNNNGTC 1 cut(s) 303
EaeI YGGCCR 2 cut(s) 592, 1113
Eam1104I CTCTTC 3 cut(s) 42, 676, 729
Eam1105I GACNNNNNGTC 1 cut(s) 303
EarI CTCTTC 3 cut(s) 42, 676, 729
Eco130I CCWWGG 1 cut(s) 526
Eco31I GGTCTC 2 cut(s) 697, 903
Eco47I GGWCC 1 cut(s) 571
Eco57I CTGAAG 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 571
EcoRI GAATTC 1 cut(s) 4
EcoT14I CCWWGG 1 cut(s) 526
ErhI CCWWGG 1 cut(s) 526
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 9 cut(s) 190, 280, 290, 374, 412, 506, 530, 758, 905
FaqI GGGAC 1 cut(s) 118
FatI CATG 9 cut(s) 186, 276, 286, 370, 408, 502, 526, 754, 901
FauI CCCGC 1 cut(s) 1010
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 4 cut(s) 65, 337, 357, 1015
FokI GGATG 2 cut(s) 965, 1029
Fsp4HI GCNGC 4 cut(s) 65, 337, 357, 1015
FspBI CTAG 3 cut(s) 12, 75, 165
GluI GCNGC 4 cut(s) 65, 337, 357, 1015
GsaI CCCAGC 1 cut(s) 1013
HaeIII GGCC 2 cut(s) 594, 1115
HapII CCGG 3 cut(s) 260, 534, 950
Hin1II CATG 9 cut(s) 190, 280, 290, 374, 412, 506, 530, 758, 905
HincII GTYRAC 2 cut(s) 18, 484
HindII GTYRAC 2 cut(s) 18, 484
HinfI GANTC 7 cut(s) 118, 715, 728, 739, 856, 876, 889
HpaI GTTAAC 1 cut(s) 18
HpaII CCGG 3 cut(s) 260, 534, 950
HphI GGTGA 5 cut(s) 140, 464, 508, 548, 712
Hpy166II GTNNAC 4 cut(s) 18, 455, 484, 599
Hpy188I TCNGA 4 cut(s) 610, 727, 733, 994
Hpy188III TCNNGA 6 cut(s) 86, 260, 308, 551, 950, 1142
Hpy8I GTNNAC 4 cut(s) 18, 455, 484, 599
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 1 cut(s) 468
HpyCH4III ACNGT 3 cut(s) 301, 385, 452
HpyCH4IV ACGT 3 cut(s) 25, 53, 601
HpyCH4V TGCA 7 cut(s) 349, 356, 412, 425, 447, 1042, 1087
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 248, 586
HpyF3I CTNAG 1 cut(s) 843
HpySE526I ACGT 3 cut(s) 25, 53, 601
Hsp92II CATG 9 cut(s) 190, 280, 290, 374, 412, 506, 530, 758, 905
Kpn2I TCCGGA 2 cut(s) 259, 949
Ksp22I TGATCA 1 cut(s) 322
KspAI GTTAAC 1 cut(s) 18
Kzo9I GATC 8 cut(s) 161, 262, 322, 530, 605, 834, 1068, 1144
LmnI GCTCC 2 cut(s) 391, 1099
Lsp1109I GCAGC 4 cut(s) 76, 348, 368, 1001
LweI GCATC 2 cut(s) 945, 1051
MaeI CTAG 3 cut(s) 12, 75, 165
MaeII ACGT 3 cut(s) 25, 53, 601
MaeIII GTNAC 5 cut(s) 49, 97, 148, 379, 496
MalI GATC 8 cut(s) 163, 264, 324, 532, 607, 836, 1070, 1146
MboI GATC 8 cut(s) 161, 262, 322, 530, 605, 834, 1068, 1144
MboII GAAGA 5 cut(s) 29, 143, 693, 746, 983
MfeI CAATTG 1 cut(s) 1082
MflI RGATCY 3 cut(s) 161, 262, 530
MhlI GDGCHC 2 cut(s) 510, 1096
MlsI TGGCCA 1 cut(s) 1115
MluCI AATT 7 cut(s) 4, 311, 361, 627, 944, 974, 1082
MluNI TGGCCA 1 cut(s) 1115
MlyI GAGTC 2 cut(s) 112, 865
MnlI CCTC 5 cut(s) 45, 189, 562, 691, 838
Mox20I TGGCCA 1 cut(s) 1115
MroI TCCGGA 2 cut(s) 259, 949
MroXI GAANNNNTTC 2 cut(s) 738, 975
MscI TGGCCA 1 cut(s) 1115
MseI TTAA 3 cut(s) 17, 774, 1150
MslI CAYNNNNRTG 2 cut(s) 375, 1063
Msp20I TGGCCA 1 cut(s) 1115
MspA1I CMGCKG 1 cut(s) 1009
MspI CCGG 3 cut(s) 260, 534, 950
MunI CAATTG 1 cut(s) 1082
MwoI GCNNNNNNNGC 3 cut(s) 64, 248, 586
NcoI CCATGG 1 cut(s) 526
NdeII GATC 8 cut(s) 161, 262, 322, 530, 605, 834, 1068, 1144
NlaIII CATG 9 cut(s) 190, 280, 290, 374, 412, 506, 530, 758, 905
NlaIV GGNNCC 2 cut(s) 532, 573
NmuCI GTSAC 4 cut(s) 97, 148, 379, 496
NspI RCATGY 2 cut(s) 290, 412
PciI ACATGT 1 cut(s) 286
PdmI GAANNNNTTC 2 cut(s) 738, 975
PfeI GAWTC 5 cut(s) 715, 728, 739, 876, 889
PkrI GCNGC 4 cut(s) 66, 338, 358, 1016
Ple19I CGATCG 1 cut(s) 837
PleI GAGTC 2 cut(s) 112, 864
PpsI GAGTC 2 cut(s) 112, 864
Ppu21I YACGTR 2 cut(s) 54, 602
PpuMI RGGWCCY 1 cut(s) 571
PscI ACATGT 1 cut(s) 286
Psp5II RGGWCCY 1 cut(s) 571
PspFI CCCAGC 1 cut(s) 1009
PspN4I GGNNCC 2 cut(s) 532, 573
PspPI GGNCC 1 cut(s) 571
PspPPI RGGWCCY 1 cut(s) 571
PsuI RGATCY 3 cut(s) 161, 262, 530
PvuI CGATCG 1 cut(s) 837
PvuII CAGCTG 1 cut(s) 1009
RsaI GTAC 1 cut(s) 750
RsaNI GTAC 1 cut(s) 749
RseI CAYNNNNRTG 2 cut(s) 375, 1063
SaqAI TTAA 3 cut(s) 17, 774, 1150
SatI GCNGC 4 cut(s) 65, 337, 357, 1015
Sau3AI GATC 8 cut(s) 161, 262, 322, 530, 605, 834, 1068, 1144
Sau96I GGNCC 1 cut(s) 571
SchI GAGTC 2 cut(s) 112, 865
SduI GDGCHC 2 cut(s) 510, 1096
SfaNI GCATC 2 cut(s) 945, 1051
SinI GGWCC 1 cut(s) 571
SmiMI CAYNNNNRTG 2 cut(s) 375, 1063
SmlI CTYRAG 1 cut(s) 743
SmoI CTYRAG 1 cut(s) 743
Sse9I AATT 7 cut(s) 4, 311, 361, 627, 944, 974, 1082
SsiI CCGC 4 cut(s) 516, 811, 934, 1017
SspI AATATT 1 cut(s) 926
SspMI CTAG 3 cut(s) 12, 75, 165
StyI CCWWGG 1 cut(s) 526
TaaI ACNGT 3 cut(s) 301, 385, 452
TaiI ACGT 3 cut(s) 28, 56, 604
TaqI TCGA 4 cut(s) 272, 837, 874, 1071
TasI AATT 7 cut(s) 4, 311, 361, 627, 944, 974, 1082
TfiI GAWTC 5 cut(s) 715, 728, 739, 876, 889
Tru1I TTAA 3 cut(s) 17, 774, 1150
Tru9I TTAA 3 cut(s) 17, 774, 1150
TscAI CASTG 4 cut(s) 157, 388, 699, 847
TseFI GTSAC 4 cut(s) 97, 148, 379, 496
TseI GCWGC 4 cut(s) 64, 336, 356, 1014
Tsp45I GTSAC 4 cut(s) 97, 148, 379, 496
TspDTI ATGAA 5 cut(s) 17, 387, 707, 1047, 1067
TspGWI ACGGA 1 cut(s) 525
TspRI CASTG 4 cut(s) 157, 388, 699, 847
VpaK11BI GGWCC 1 cut(s) 571
XapI RAATTY 2 cut(s) 4, 974
XceI RCATGY 2 cut(s) 290, 412
XmnI GAANNNNTTC 2 cut(s) 738, 975
XspI CTAG 3 cut(s) 12, 75, 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.