Rroxscaffold_1G00061720

threonine-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0021435
Physical Location & Seq
Reverse (-)
83786423 .. 83790041
3619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061720.1

Sequence Viewer

Length: 1215 bp
ATGAATCTCTGCACTCCAACAACTCGTTTCGGCGGGCTTCCAAAAAAATCAAAGAATTCTGAGGCCAATCAGGAGGTTAACAATATGTCCAAACCTTCATCCTCTCATCATTCACATGGTCGTTGCAGCTCACCTCTAGGTTTGCAAGTGACAGGGACGACAACCTTGGCTCTCATCGGTGAAGATGTGATATATGTGGTTACTGACCAGAGAAGCACTTACAAGCCACCTCCTTTCATGATAGCGGATGAAAGTTGCTCAAAAACTTTTGTCATTTCAAGCAATGTGTTGGCTACATGGGCTGGAAATCCGCGTATTTGTTCGGACTTGTGTATAGATGTAAAGTCGCATGTCAATCAAGATATAATTGCTGGTAAATTACAGAGCGGCAATGTTGCGCGTGCTGCAGCAACTTATGGTCGTAAGTGGATCACTAGATGGGAGGATCAGCGTCCGAACATGACCTTTTCGTGTCAAATAATGATAGTTGGTTGGTCTGATGGTAAATGTGTAGCCTTTGACCTTCAATCTCCCAAATTCCATCAACTACATGTCAAACATGCTCATGGAGATGGATTTACTCATGGATCCGGTGCGGATTATGTTAAAGCTTATTATCGAATTGAGAACCCAATGCCATACAATCAAAGTCTTCCTCTTCCACATAGATCAAACAACGAACTTGCCGAAATTGGAAAAAGAGCGGTGGTGTATTCAGCATTACATGTTCGATCTGCTGGAGGAAGAGTTGGTTTGGTGGAGGTGGGACAAACTATGATTCATGAGTCAGATCCTAAAGAGATTCTTGAGGTACTACGTGATGATTATGACAAGTTTAAAACCTGCTTATCATGTTTCCTCTTCTGTCTTTACCGCATGAGTGATTTTGAATATGCATCCGACACTGAGGCACTTTTGGCTCAAGAAATCTACAATCGATTCACTCCTAAGATTTTAAGCTCCCATGTGATTCAGATCAAGGAAAAATACTTTATCCGCATCTTACAATTTGAGAATGAAAAATTTGCTAAAGAAGAATATCAGGCTATAAATTTGGAGTGGAAACAACAGCGGGGCTACGGGCATACTCAAGATGTGGATGTGCATCCCAATATTCGAGCATTGCCGTGGATCGAGCTTCATCGATTGCAGGGTACTCCATTTGTCTTTGCTATGCCCACTAAAAAGTATGTTAGTCTTATTCTTGATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 ko03050 map03008 map03050
Pfam Domains
Protein Families
No domains found.

Protein Analysis

404

Amino Acids

45.74

Weight (kDa)

8.23

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021435)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0014851
rosa_laevigata RLG00000032148
rosa_roxburghii Rroxscaffold_1G00061720
rosa_samantha Rh5BG113600 Rh5CG125300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 851
AccBSI CCGCTC 2 cut(s) 387, 704
AccII CGCG 2 cut(s) 313, 400
AciI CCGC 9 cut(s) 33, 245, 311, 387, 596, 704, 874, 997, 1072
AclWI GGATC 6 cut(s) 437, 453, 582, 595, 785, 1139
AcsI RAATTY 4 cut(s) 55, 536, 1022, 1051
AfaI GTAC 2 cut(s) 813, 1156
AflIII ACRYGT 2 cut(s) 550, 724
AgsI TTSAA 3 cut(s) 279, 527, 890
AluBI AGCT 4 cut(s) 129, 611, 960, 1138
AluI AGCT 4 cut(s) 129, 611, 960, 1138
AlwI GGATC 6 cut(s) 437, 453, 582, 595, 785, 1139
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 3 cut(s) 126, 404, 407
ApoI RAATTY 4 cut(s) 55, 536, 1022, 1051
AspLEI GCGC 1 cut(s) 400
AsuHPI GGTGA 2 cut(s) 123, 191
BamHI GGATCC 1 cut(s) 587
BbsI GAAGAC 1 cut(s) 644
BbvI GCAGC 3 cut(s) 138, 391, 419
BccI CCATC 4 cut(s) 432, 494, 549, 566
BceAI ACGGC 1 cut(s) 1111
BfaI CTAG 2 cut(s) 137, 435
BfmI CTRYAG 1 cut(s) 405
BfuAI ACCTGC 1 cut(s) 851
BisI GCNGC 4 cut(s) 127, 388, 405, 408
BlsI GCNGC 4 cut(s) 128, 389, 406, 409
BmiI GGNNCC 1 cut(s) 589
BmsI GCATC 3 cut(s) 905, 1008, 1114
BpiI GAAGAC 1 cut(s) 644
BpmI CTGGAG 1 cut(s) 759
BpuEI CTTGAG 3 cut(s) 827, 906, 1074
Bsa29I ATCGAT 2 cut(s) 937, 1144
BsaAI YACGTR 1 cut(s) 818
BsaBI GATNNNNATC 2 cut(s) 974, 1104
BsaJI CCNNGG 2 cut(s) 165, 1127
BsaWI WCCGGW 1 cut(s) 590
BsaXI ACNNNNNCTCC 2 cut(s) 732, 762
Bse3DI GCAATG 3 cut(s) 289, 397, 1121
Bse8I GATNNNNATC 2 cut(s) 974, 1104
BseCI ATCGAT 2 cut(s) 937, 1144
BseDI CCNNGG 2 cut(s) 165, 1127
BseGI GGATG 5 cut(s) 98, 253, 896, 1105, 1105
BseJI GATNNNNATC 2 cut(s) 974, 1104
BseMI GCAATG 3 cut(s) 289, 397, 1121
BseMII CTCAG 2 cut(s) 51, 897
BseXI GCAGC 3 cut(s) 138, 391, 419
Bsh1236I CGCG 2 cut(s) 313, 400
BshFI GGCC 1 cut(s) 65
BshVI ATCGAT 2 cut(s) 937, 1144
BsiSI CCGG 1 cut(s) 591
BslFI GGGAC 2 cut(s) 169, 780
BsmFI GGGAC 2 cut(s) 169, 780
BsnI GGCC 1 cut(s) 65
Bsp143I GATC 9 cut(s) 429, 445, 587, 668, 731, 790, 975, 1131, 1207
BspACI CCGC 9 cut(s) 33, 245, 311, 387, 596, 704, 874, 997, 1072
BspANI GGCC 1 cut(s) 65
BspCNI CTCAG 2 cut(s) 52, 898
BspDI ATCGAT 2 cut(s) 937, 1144
BspFNI CGCG 2 cut(s) 313, 400
BspHI TCATGA 2 cut(s) 237, 781
BspLI GGNNCC 1 cut(s) 589
BspMAI CTGCAG 1 cut(s) 409
BspMI ACCTGC 1 cut(s) 851
BspPI GGATC 6 cut(s) 437, 453, 582, 595, 785, 1139
BsrBI CCGCTC 2 cut(s) 387, 704
BsrDI GCAATG 3 cut(s) 289, 397, 1121
BssECI CCNNGG 2 cut(s) 165, 1127
BssMI GATC 9 cut(s) 429, 445, 587, 668, 731, 790, 975, 1131, 1207
BssT1I CCWWGG 1 cut(s) 165
Bst6I CTCTTC 3 cut(s) 663, 739, 866
BstBAI YACGTR 1 cut(s) 818
BstC8I GCNNGC 2 cut(s) 35, 402
BstDEI CTNAG 3 cut(s) 60, 906, 948
BstDSI CCRYGG 1 cut(s) 1127
BstF5I GGATG 5 cut(s) 98, 253, 896, 1105, 1105
BstFNI CGCG 2 cut(s) 313, 400
BstHHI GCGC 1 cut(s) 400
BstKTI GATC 9 cut(s) 432, 448, 590, 671, 734, 793, 978, 1134, 1210
BstMBI GATC 9 cut(s) 429, 445, 587, 668, 731, 790, 975, 1131, 1207
BstMWI GCNNNNNNNGC 3 cut(s) 299, 404, 917
BstNSI RCATGY 4 cut(s) 353, 554, 563, 728
BstSFI CTRYAG 1 cut(s) 405
BstUI CGCG 2 cut(s) 313, 400
BstV1I GCAGC 3 cut(s) 138, 391, 419
BstV2I GAAGAC 1 cut(s) 644
BstX2I RGATCY 2 cut(s) 587, 790
BstYI RGATCY 2 cut(s) 587, 790
Bsu15I ATCGAT 2 cut(s) 937, 1144
BsuRI GGCC 1 cut(s) 65
BsuTUI ATCGAT 2 cut(s) 937, 1144
BtgI CCRYGG 1 cut(s) 1127
BtsCI GGATG 5 cut(s) 98, 253, 896, 1105, 1105
BtsIMutI CAGTG 1 cut(s) 903
BveI ACCTGC 1 cut(s) 851
Cac8I GCNNGC 2 cut(s) 35, 402
CciI TCATGA 2 cut(s) 237, 781
CfoI GCGC 1 cut(s) 400
ClaI ATCGAT 2 cut(s) 937, 1144
CseI GACGC 1 cut(s) 440
Csp6I GTAC 2 cut(s) 812, 1155
CviQI GTAC 2 cut(s) 812, 1155
DdeI CTNAG 3 cut(s) 60, 906, 948
DpnI GATC 9 cut(s) 431, 447, 589, 670, 733, 792, 977, 1133, 1209
DpnII GATC 9 cut(s) 429, 445, 587, 668, 731, 790, 975, 1131, 1207
DraI TTTAAA 1 cut(s) 838
Eam1104I CTCTTC 3 cut(s) 663, 739, 866
EarI CTCTTC 3 cut(s) 663, 739, 866
Eco130I CCWWGG 1 cut(s) 165
EcoRI GAATTC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 165
EcoT22I ATGCAT 1 cut(s) 898
ErhI CCWWGG 1 cut(s) 165
FalI AAGNNNNNCTT 2 cut(s) 789, 821
FaqI GGGAC 2 cut(s) 169, 780
FauI CCCGC 2 cut(s) 26, 1065
Fnu4HI GCNGC 4 cut(s) 127, 388, 405, 408
FokI GGATG 5 cut(s) 85, 260, 883, 1092, 1112
Fsp4HI GCNGC 4 cut(s) 127, 388, 405, 408
FspBI CTAG 2 cut(s) 137, 435
GlaI GCGC 1 cut(s) 399
GluI GCNGC 4 cut(s) 127, 388, 405, 408
GsuI CTGGAG 1 cut(s) 759
HaeIII GGCC 1 cut(s) 65
HapII CCGG 1 cut(s) 591
HgaI GACGC 1 cut(s) 440
HhaI GCGC 1 cut(s) 400
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 1 cut(s) 609
HinfI GANTC 6 cut(s) 4, 778, 785, 802, 939, 970
HpaI GTTAAC 1 cut(s) 79
HpaII CCGG 1 cut(s) 591
HphI GGTGA 2 cut(s) 123, 191
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 7 cut(s) 61, 325, 456, 499, 790, 901, 975
Hpy188III TCNNGA 8 cut(s) 71, 238, 359, 782, 806, 923, 1091, 1205
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 2 cut(s) 105, 533
HpyCH4IV ACGT 1 cut(s) 817
HpyCH4V TGCA 7 cut(s) 12, 126, 145, 407, 896, 1105, 1150
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 404, 917
HpyF3I CTNAG 3 cut(s) 60, 906, 948
HpySE526I ACGT 1 cut(s) 817
HspAI GCGC 1 cut(s) 398
KspAI GTTAAC 1 cut(s) 79
Kzo9I GATC 9 cut(s) 429, 445, 587, 668, 731, 790, 975, 1131, 1207
LmnI GCTCC 1 cut(s) 965
Lsp1109I GCAGC 3 cut(s) 138, 391, 419
LweI GCATC 3 cut(s) 905, 1008, 1114
MaeI CTAG 2 cut(s) 137, 435
MaeII ACGT 1 cut(s) 817
MaeIII GTNAC 2 cut(s) 148, 199
MalI GATC 9 cut(s) 431, 447, 589, 670, 733, 792, 977, 1133, 1209
MbiI CCGCTC 2 cut(s) 387, 704
MboI GATC 9 cut(s) 429, 445, 587, 668, 731, 790, 975, 1131, 1207
MboII GAAGA 6 cut(s) 194, 644, 650, 756, 853, 1046
MflI RGATCY 2 cut(s) 587, 790
MluCI AATT 9 cut(s) 55, 366, 377, 536, 621, 690, 1007, 1022, 1051
MlyI GAGTC 1 cut(s) 794
MmeI TCCRAC 2 cut(s) 41, 924
Mph1103I ATGCAT 1 cut(s) 898
MseI TTAA 4 cut(s) 78, 606, 837, 956
MslI CAYNNNNRTG 4 cut(s) 114, 564, 570, 1126
MspA1I CMGCKG 1 cut(s) 1072
MspI CCGG 1 cut(s) 591
MvnI CGCG 2 cut(s) 313, 400
MwoI GCNNNNNNNGC 3 cut(s) 299, 404, 917
NdeII GATC 9 cut(s) 429, 445, 587, 668, 731, 790, 975, 1131, 1207
NlaIV GGNNCC 1 cut(s) 589
NmuCI GTSAC 1 cut(s) 148
NsiI ATGCAT 1 cut(s) 898
NspI RCATGY 4 cut(s) 353, 554, 563, 728
PagI TCATGA 2 cut(s) 237, 781
PciI ACATGT 2 cut(s) 550, 724
PcsI WCGNNNNNNNCGW 1 cut(s) 684
PfeI GAWTC 5 cut(s) 4, 778, 802, 939, 970
PkrI GCNGC 4 cut(s) 128, 389, 406, 409
PleI GAGTC 1 cut(s) 793
PpsI GAGTC 1 cut(s) 793
Ppu21I YACGTR 1 cut(s) 818
PscI ACATGT 2 cut(s) 550, 724
PspN4I GGNNCC 1 cut(s) 589
PstI CTGCAG 1 cut(s) 409
PsuI RGATCY 2 cut(s) 587, 790
RsaI GTAC 2 cut(s) 813, 1156
RsaNI GTAC 2 cut(s) 812, 1155
RseI CAYNNNNRTG 4 cut(s) 114, 564, 570, 1126
SaqAI TTAA 4 cut(s) 78, 606, 837, 956
SatI GCNGC 4 cut(s) 127, 388, 405, 408
Sau3AI GATC 9 cut(s) 429, 445, 587, 668, 731, 790, 975, 1131, 1207
SchI GAGTC 1 cut(s) 794
SfaNI GCATC 3 cut(s) 905, 1008, 1114
SfcI CTRYAG 1 cut(s) 405
SmiMI CAYNNNNRTG 4 cut(s) 114, 564, 570, 1126
SmlI CTYRAG 3 cut(s) 806, 921, 1089
SmoI CTYRAG 3 cut(s) 806, 921, 1089
Sse9I AATT 9 cut(s) 55, 366, 377, 536, 621, 690, 1007, 1022, 1051
SsiI CCGC 9 cut(s) 33, 245, 311, 387, 596, 704, 874, 997, 1072
SspI AATATT 1 cut(s) 1114
SspMI CTAG 2 cut(s) 137, 435
StyI CCWWGG 1 cut(s) 165
TaiI ACGT 1 cut(s) 820
TaqI TCGA 6 cut(s) 619, 730, 937, 1117, 1134, 1144
TasI AATT 9 cut(s) 55, 366, 377, 536, 621, 690, 1007, 1022, 1051
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 5 cut(s) 4, 778, 802, 939, 970
Tru1I TTAA 4 cut(s) 78, 606, 837, 956
Tru9I TTAA 4 cut(s) 78, 606, 837, 956
TscAI CASTG 1 cut(s) 910
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 3 cut(s) 126, 404, 407
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 7 cut(s) 17, 87, 226, 264, 770, 1032, 1130
TspRI CASTG 1 cut(s) 910
XapI RAATTY 4 cut(s) 55, 536, 1022, 1051
XceI RCATGY 4 cut(s) 353, 554, 563, 728
XspI CTAG 2 cut(s) 137, 435
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.