RchiOBHm_Chr5g0017751
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0021439
Physical Location & Seq
Reverse (-)
12349518 .. 12350432
915 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29800

Sequence Viewer

Length: 915 bp
ATGTCGTCGACCCAGTTCTCCCTGGCCAAGCTCCGCGCGGCCACCAACAACTTCTCTACTGATAATGTCATTGGAACTGGGAGGAGCGGCCTGATCTACAAGGCCCATCTCTCCAATGGACTCACCGTCGCCATCAAGAACTACGACCCCGATCGATTCCAAGGCTTTCGGGAATTCCGGGCTGAAGCCGAAGCGCTAGGCAAGCTTCGACACCCAAATATCGTTAAGCTTTTGAGTTATCAAGCCTCCGGCTCAGTTATGCTTCTTGTTTATGAGTATGTAGAGAGAGGTAACCTTCACGAACGTCTTCGTGAAAGTTGCTCTTTTTCATGGTTTGACAAGTTTAAGATTCTTAGAGGTGTAGCCAATGGTCTCGCTTATTTGCATGGGTTGGAAAACCCTGTCATTCATAGAGATATTGCGTCTAGAAATGTGCTGGTGGACTCAGACTATGAGCCTCGTATTATTAATTTTGGGCTTTCTAGGACTATTGACAAGTGTCATTCGCTTTCTCATGTGTCAACGGCAGTTGCTGGGTCCGTACCGTACATGGCGCCGGAGTACAGGAGTGGAAGCACCAAAGCCACGGTGAAAGGCGATGTGTATAGTTTTGGCATTTTGATGATGGAGACTGTATTAGGACCCACAAGGTGTAGTAGATATTTCAAAGTTTCAGCTCAAAACAGAGATTTTGATCTTGTAGAATGGGTTAGGGGTATAGAGTTGGACAAGATCATAAGCGACTTTTCTGGCTATTCTGGCTATTTCGTCGATGCTCTATGGCCCGAGTATATTCGGATCGCCTTATTGTGTGCTAATGATAATCCAGAAGACAGGCCTGTGATGAACGAGGTTGTTCAAATGCTAAATCAAATTCTACCTCATACCCCTGCTTCCTCCCGGAGCACCGGTTAA

Protein Analysis

304

Amino Acids

34.09

Weight (kDa)

8.27

Isoelectric Point (pI)

25.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 19 - 287 6.6e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 20 - 289 2.5e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021439)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0017751
rosa_laevigata RLG00000032311
rosa_rugosa Rorug05G0038300
rosa_samantha Rh5BG129300 Rh5CG141700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 553
AccBSI CCGCTC 1 cut(s) 87
AccI GTMKAC 1 cut(s) 8
AccII CGCG 2 cut(s) 36, 38
AciI CCGC 3 cut(s) 34, 38, 87
AclWI GGATC 1 cut(s) 806
AcoI YGGCCR 2 cut(s) 24, 39
AcsI RAATTY 2 cut(s) 173, 873
AcuI CTGAAG 1 cut(s) 204
AcyI GRCGYC 1 cut(s) 554
AdeI CACNNNGTG 1 cut(s) 651
AfaI GTAC 3 cut(s) 543, 548, 563
AfeI AGCGCT 1 cut(s) 195
AgeI ACCGGT 1 cut(s) 908
AgsI TTSAA 2 cut(s) 667, 860
AhdI GACNNNNNGTC 1 cut(s) 125
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 4 cut(s) 31, 205, 229, 677
AluI AGCT 4 cut(s) 31, 205, 229, 677
Alw21I GWGCWC 1 cut(s) 908
Alw26I GTCTC 2 cut(s) 377, 623
AlwI GGATC 1 cut(s) 806
AlwNI CAGNNNCTG 1 cut(s) 533
Ama87I CYCGRG 1 cut(s) 785
Aor51HI AGCGCT 1 cut(s) 195
AoxI GGCC 6 cut(s) 24, 39, 88, 102, 782, 836
ApoI RAATTY 2 cut(s) 173, 873
AseI ATTAAT 1 cut(s) 468
AsiGI ACCGGT 1 cut(s) 908
Asp700I GAANNNNTTC 1 cut(s) 306
AspLEI GCGC 3 cut(s) 38, 196, 556
AspS9I GGNCC 4 cut(s) 103, 537, 641, 783
AsuC2I CCSGG 2 cut(s) 179, 901
AsuHPI GGTGA 2 cut(s) 115, 601
AvaI CYCGRG 1 cut(s) 785
AvaII GGWCC 2 cut(s) 537, 641
BalI TGGCCA 1 cut(s) 26
BanI GGYRCC 1 cut(s) 553
BbsI GAAGAC 2 cut(s) 299, 837
Bbv12I GWGCWC 1 cut(s) 908
BccI CCATC 3 cut(s) 114, 140, 619
BceAI ACGGC 1 cut(s) 540
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 2 cut(s) 179, 901
BcoDI GTCTC 2 cut(s) 377, 623
BfaI CTAG 3 cut(s) 197, 426, 483
BfoI RGCGCY 2 cut(s) 197, 557
BisI GCNGC 2 cut(s) 39, 88
BlsI GCNGC 2 cut(s) 40, 89
Bme1390I CCNGG 3 cut(s) 23, 179, 901
Bme18I GGWCC 2 cut(s) 537, 641
BmeRI GACNNNNNGTC 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 785
BmgT120I GGNCC 4 cut(s) 103, 537, 641, 783
BmiI GGNNCC 3 cut(s) 538, 555, 643
BmrFI CCNGG 3 cut(s) 23, 179, 901
BmrI ACTGGG 2 cut(s) 7, 87
BmsI GCATC 1 cut(s) 763
BmuI ACTGGG 2 cut(s) 7, 87
BoxI GACNNNNGTC 1 cut(s) 498
BpiI GAAGAC 2 cut(s) 299, 837
BpuMI CCSGG 2 cut(s) 179, 901
Bsa29I ATCGAT 1 cut(s) 154
BsaBI GATNNNNATC 1 cut(s) 693
BsaHI GRCGYC 1 cut(s) 554
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 3 cut(s) 21, 160, 585
BsaWI WCCGGW 1 cut(s) 908
BsaXI ACNNNNNCTCC 1 cut(s) 895
Bse118I RCCGGY 1 cut(s) 908
Bse1I ACTGG 2 cut(s) 13, 82
Bse8I GATNNNNATC 1 cut(s) 693
BseBI CCWGG 1 cut(s) 23
BseCI ATCGAT 1 cut(s) 154
BseDI CCNNGG 3 cut(s) 21, 160, 585
BseJI GATNNNNATC 1 cut(s) 693
BseMII CTCAG 2 cut(s) 267, 459
BseNI ACTGG 2 cut(s) 13, 82
BseRI GAGGAG 1 cut(s) 97
BseYI CCCAGC 1 cut(s) 533
Bsh1236I CGCG 2 cut(s) 36, 38
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 6 cut(s) 26, 41, 90, 104, 784, 838
BshNI GGYRCC 1 cut(s) 553
BshTI ACCGGT 1 cut(s) 908
BshVI ATCGAT 1 cut(s) 154
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 908
BsiHKCI CYCGRG 1 cut(s) 785
BsiSI CCGG 5 cut(s) 178, 249, 557, 901, 909
BsmAI GTCTC 2 cut(s) 377, 623
BsnI GGCC 6 cut(s) 26, 41, 90, 104, 784, 838
Bso31I GGTCTC 1 cut(s) 377
BsoBI CYCGRG 1 cut(s) 785
Bsp1286I GDGCHC 1 cut(s) 908
Bsp143I GATC 5 cut(s) 93, 151, 694, 732, 798
BspACI CCGC 3 cut(s) 34, 38, 87
BspANI GGCC 6 cut(s) 26, 41, 90, 104, 784, 838
BspCNI CTCAG 2 cut(s) 266, 458
BspDI ATCGAT 1 cut(s) 154
BspFNI CGCG 2 cut(s) 36, 38
BspLI GGNNCC 3 cut(s) 538, 555, 643
BspPI GGATC 1 cut(s) 806
BspT107I GGYRCC 1 cut(s) 553
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 908
BsrI ACTGG 2 cut(s) 13, 82
BssAI RCCGGY 1 cut(s) 908
BssECI CCNNGG 3 cut(s) 21, 160, 585
BssMI GATC 5 cut(s) 93, 151, 694, 732, 798
BssNI GRCGYC 1 cut(s) 554
BssT1I CCWWGG 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 4 cut(s) 127, 546, 589, 634
BstACI GRCGYC 1 cut(s) 554
BstC8I GCNNGC 1 cut(s) 203
BstDEI CTNAG 3 cut(s) 253, 353, 445
BstDSI CCRYGG 1 cut(s) 585
BstEII GGTNACC 1 cut(s) 290
BstFNI CGCG 2 cut(s) 36, 38
BstH2I RGCGCY 2 cut(s) 197, 557
BstHHI GCGC 3 cut(s) 38, 196, 556
BstKTI GATC 5 cut(s) 96, 154, 697, 735, 801
BstMAI GTCTC 2 cut(s) 377, 623
BstMBI GATC 5 cut(s) 93, 151, 694, 732, 798
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 2 cut(s) 202, 759
BstNI CCWGG 1 cut(s) 23
BstPAI GACNNNNGTC 1 cut(s) 498
BstPI GGTNACC 1 cut(s) 290
BstSCI CCNGG 3 cut(s) 21, 177, 899
BstUI CGCG 2 cut(s) 36, 38
BstV2I GAAGAC 2 cut(s) 299, 837
Bsu15I ATCGAT 1 cut(s) 154
BsuRI GGCC 6 cut(s) 26, 41, 90, 104, 784, 838
BsuTUI ATCGAT 1 cut(s) 154
BtgI CCRYGG 1 cut(s) 585
BtgZI GCGATG 1 cut(s) 612
Cac8I GCNNGC 1 cut(s) 203
CaiI CAGNNNCTG 1 cut(s) 533
CfoI GCGC 3 cut(s) 38, 196, 556
Cfr10I RCCGGY 1 cut(s) 908
Cfr13I GGNCC 4 cut(s) 103, 537, 641, 783
ClaI ATCGAT 1 cut(s) 154
CseI GACGC 1 cut(s) 411
Csp6I GTAC 3 cut(s) 542, 547, 562
CspAI ACCGGT 1 cut(s) 908
CviAII CATG 4 cut(s) 330, 386, 515, 550
CviQI GTAC 3 cut(s) 542, 547, 562
DdeI CTNAG 3 cut(s) 253, 353, 445
DinI GGCGCC 1 cut(s) 555
DpnI GATC 5 cut(s) 95, 153, 696, 734, 800
DpnII GATC 5 cut(s) 93, 151, 694, 732, 798
DraIII CACNNNGTG 1 cut(s) 651
DriI GACNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 2 cut(s) 24, 39
Eam1105I GACNNNNNGTC 1 cut(s) 125
Eco130I CCWWGG 1 cut(s) 160
Eco147I AGGCCT 1 cut(s) 838
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 2 cut(s) 537, 641
Eco47III AGCGCT 1 cut(s) 195
Eco57I CTGAAG 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 785
Eco91I GGTNACC 1 cut(s) 290
EcoO109I RGGNCCY 1 cut(s) 641
EcoO65I GGTNACC 1 cut(s) 290
EcoRI GAATTC 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 160
EgeI GGCGCC 1 cut(s) 555
EheI GGCGCC 1 cut(s) 555
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 4 cut(s) 333, 389, 518, 553
FalI AAGNNNNNCTT 2 cut(s) 307, 339
FatI CATG 4 cut(s) 329, 385, 514, 549
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 2 cut(s) 39, 88
Fsp4HI GCNGC 2 cut(s) 39, 88
FspBI CTAG 3 cut(s) 197, 426, 483
GlaI GCGC 3 cut(s) 37, 195, 555
GluI GCNGC 2 cut(s) 39, 88
GsaI CCCAGC 1 cut(s) 537
HaeII RGCGCY 2 cut(s) 197, 557
HaeIII GGCC 6 cut(s) 26, 41, 90, 104, 784, 838
HapII CCGG 5 cut(s) 178, 249, 557, 901, 909
HgaI GACGC 1 cut(s) 411
HhaI GCGC 3 cut(s) 38, 196, 556
Hin1I GRCGYC 1 cut(s) 554
Hin1II CATG 4 cut(s) 333, 389, 518, 553
Hin6I GCGC 3 cut(s) 36, 194, 554
HinP1I GCGC 3 cut(s) 36, 194, 554
HincII GTYRAC 2 cut(s) 9, 522
HindII GTYRAC 2 cut(s) 9, 522
HindIII AAGCTT 2 cut(s) 203, 227
HinfI GANTC 4 cut(s) 120, 156, 349, 443
HpaII CCGG 5 cut(s) 178, 249, 557, 901, 909
HphI GGTGA 2 cut(s) 115, 601
Hpy166II GTNNAC 3 cut(s) 9, 442, 522
Hpy188I TCNGA 2 cut(s) 448, 798
Hpy188III TCNNGA 6 cut(s) 136, 170, 299, 311, 426, 827
Hpy8I GTNNAC 3 cut(s) 9, 442, 522
Hpy99I CGWCG 3 cut(s) 10, 131, 773
HpyAV CCTTC 1 cut(s) 305
HpyCH4III ACNGT 4 cut(s) 127, 546, 589, 634
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 1 cut(s) 385
HpyF10VI GCNNNNNNNGC 2 cut(s) 202, 759
HpyF3I CTNAG 3 cut(s) 253, 353, 445
HpySE526I ACGT 1 cut(s) 304
Hsp92I GRCGYC 1 cut(s) 554
Hsp92II CATG 4 cut(s) 333, 389, 518, 553
HspAI GCGC 3 cut(s) 36, 194, 554
KasI GGCGCC 1 cut(s) 553
Kzo9I GATC 5 cut(s) 93, 151, 694, 732, 798
LmnI GCTCC 3 cut(s) 36, 84, 903
LweI GCATC 1 cut(s) 763
MaeI CTAG 3 cut(s) 197, 426, 483
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 1 cut(s) 290
MalI GATC 5 cut(s) 95, 153, 696, 734, 800
MbiI CCGCTC 1 cut(s) 87
MboI GATC 5 cut(s) 93, 151, 694, 732, 798
MboII GAAGA 2 cut(s) 299, 842
MhlI GDGCHC 1 cut(s) 908
MlsI TGGCCA 1 cut(s) 26
MluCI AATT 3 cut(s) 173, 469, 873
MluNI TGGCCA 1 cut(s) 26
Mly113I GGCGCC 1 cut(s) 554
MlyI GAGTC 2 cut(s) 114, 437
MmeI TCCRAC 2 cut(s) 372, 705
MnlI CCTC 8 cut(s) 75, 256, 281, 350, 468, 844, 891, 907
Mox20I TGGCCA 1 cut(s) 26
MroXI GAANNNNTTC 1 cut(s) 306
MscI TGGCCA 1 cut(s) 26
MseI TTAA 4 cut(s) 225, 345, 468, 913
MslI CAYNNNNRTG 1 cut(s) 620
Msp20I TGGCCA 1 cut(s) 26
MspI CCGG 5 cut(s) 178, 249, 557, 901, 909
MspR9I CCNGG 3 cut(s) 23, 179, 901
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 2 cut(s) 36, 38
MwoI GCNNNNNNNGC 2 cut(s) 202, 759
NarI GGCGCC 1 cut(s) 554
NciI CCSGG 2 cut(s) 179, 901
NdeII GATC 5 cut(s) 93, 151, 694, 732, 798
NlaIII CATG 4 cut(s) 333, 389, 518, 553
NlaIV GGNNCC 3 cut(s) 538, 555, 643
PceI AGGCCT 1 cut(s) 838
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 2 cut(s) 156, 349
PfoI TCCNGGA 1 cut(s) 899
PinAI ACCGGT 1 cut(s) 908
PkrI GCNGC 2 cut(s) 40, 89
Ple19I CGATCG 1 cut(s) 154
PleI GAGTC 2 cut(s) 114, 437
PluTI GGCGCC 1 cut(s) 557
PpsI GAGTC 2 cut(s) 114, 437
PpuMI RGGWCCY 1 cut(s) 641
PshAI GACNNNNGTC 1 cut(s) 498
PshBI ATTAAT 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 641
Psp6I CCWGG 1 cut(s) 21
PspEI GGTNACC 1 cut(s) 290
PspFI CCCAGC 1 cut(s) 533
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 3 cut(s) 538, 555, 643
PspPI GGNCC 4 cut(s) 103, 537, 641, 783
PspPPI RGGWCCY 1 cut(s) 641
PstNI CAGNNNCTG 1 cut(s) 533
PvuI CGATCG 1 cut(s) 154
RsaI GTAC 3 cut(s) 543, 548, 563
RsaNI GTAC 3 cut(s) 542, 547, 562
RseI CAYNNNNRTG 1 cut(s) 620
SalI GTCGAC 1 cut(s) 7
SaqAI TTAA 4 cut(s) 225, 345, 468, 913
SatI GCNGC 2 cut(s) 39, 88
Sau3AI GATC 5 cut(s) 93, 151, 694, 732, 798
Sau96I GGNCC 4 cut(s) 103, 537, 641, 783
SchI GAGTC 2 cut(s) 114, 437
ScrFI CCNGG 3 cut(s) 23, 179, 901
SduI GDGCHC 1 cut(s) 908
SfaNI GCATC 1 cut(s) 763
SfoI GGCGCC 1 cut(s) 555
SinI GGWCC 2 cut(s) 537, 641
SmiMI CAYNNNNRTG 1 cut(s) 620
Sse9I AATT 3 cut(s) 173, 469, 873
SseBI AGGCCT 1 cut(s) 838
SsiI CCGC 3 cut(s) 34, 38, 87
SspDI GGCGCC 1 cut(s) 553
SspMI CTAG 3 cut(s) 197, 426, 483
StuI AGGCCT 1 cut(s) 838
StyD4I CCNGG 3 cut(s) 21, 177, 899
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 4 cut(s) 127, 546, 589, 634
TaiI ACGT 1 cut(s) 307
TaqI TCGA 4 cut(s) 8, 154, 208, 771
TasI AATT 3 cut(s) 173, 469, 873
TatI WGTACW 1 cut(s) 561
TauI GCSGC 2 cut(s) 41, 90
TfiI GAWTC 2 cut(s) 156, 349
Tru1I TTAA 4 cut(s) 225, 345, 468, 913
Tru9I TTAA 4 cut(s) 225, 345, 468, 913
TspDTI ATGAA 3 cut(s) 318, 398, 860
TspGWI ACGGA 1 cut(s) 529
VpaK11BI GGWCC 2 cut(s) 537, 641
VspI ATTAAT 1 cut(s) 468
XapI RAATTY 2 cut(s) 173, 873
XbaI TCTAGA 1 cut(s) 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 3 cut(s) 197, 426, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.