RchiOBHm_Chr5g0081271

NO-associated protein 1, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010230
Physical Location & Seq
Forward (+)
87251466 .. 87252464
999 bp
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UTR
Exon/CDS
Intron
PRQ35552

Sequence Viewer

Length: 450 bp
ATGAGGATGGATATAGAGGAATCATCGGTAGATTCTGTTTATGTTACAGCATGGGCGTCTCCTTATCTTCCATTACACATGGGGAAAATGGAAAACGCATCCGAAATGGTAGAGAAACACTTTGGTCGTCAGTTACATCCACCAATCGGAGAGAATAGAGTGAAGGAGCTTGGACAGTGGGTGAAAAAGGAGTTTCACATCAGTGGAAACGGCTGGGATTCAAGTTCGGTGGACATTGCTGCTGCAGGTCTTGGCTGGTTTGCTGTTGGACTTAAAGGAGAGGCAGTTTTGAGTGTTTGGAATTATGATGGAATCGATGTTGTTCTTCGCAATTCATTGCTTCCTAATAGATCATACAATTTTGAAGTTTCCGGGTTTACAGTTGATAGGAGCATATTTATGCTACGTTATTATTATTTAACCCTATGTTTTTCTTTGCTAGTTTTCTGA

Protein Analysis

149

Amino Acids

16.93

Weight (kDa)

5.51

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YqeH-like_C PF21516 16 - 113 2.2e-14 YqeH-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 236
AcyI GRCGYC 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 2 cut(s) 222, 365
AleI CACNNNNGTG 1 cut(s) 201
AluBI AGCT 1 cut(s) 169
AluI AGCT 1 cut(s) 169
Alw26I GTCTC 1 cut(s) 63
ApeKI GCWGC 2 cut(s) 239, 242
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 1 cut(s) 193
BbvI GCAGC 2 cut(s) 226, 229
BccI CCATC 1 cut(s) 302
BceAI ACGGC 1 cut(s) 226
BcnI CCSGG 1 cut(s) 373
BcoDI GTCTC 1 cut(s) 63
BfaI CTAG 1 cut(s) 440
BfmI CTRYAG 1 cut(s) 243
BfuAI ACCTGC 1 cut(s) 236
BisI GCNGC 2 cut(s) 240, 243
BlsI GCNGC 2 cut(s) 241, 244
Bme1390I CCNGG 1 cut(s) 373
BmrFI CCNGG 1 cut(s) 373
BmsI GCATC 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 373
Bsa29I ATCGAT 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 270, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse3DI GCAATG 2 cut(s) 234, 335
BseCI ATCGAT 1 cut(s) 315
BseGI GGATG 3 cut(s) 12, 98, 136
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 2 cut(s) 234, 335
BseXI GCAGC 2 cut(s) 226, 229
BseYI CCCAGC 1 cut(s) 213
BshVI ATCGAT 1 cut(s) 315
BsiSI CCGG 1 cut(s) 372
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 63
BsmBI CGTCTC 1 cut(s) 63
Bsp143I GATC 1 cut(s) 350
BspDI ATCGAT 1 cut(s) 315
BspMAI CTGCAG 1 cut(s) 247
BspMI ACCTGC 1 cut(s) 236
BsrDI GCAATG 2 cut(s) 234, 335
BssMI GATC 1 cut(s) 350
BssNI GRCGYC 1 cut(s) 56
Bst4CI ACNGT 2 cut(s) 177, 382
BstACI GRCGYC 1 cut(s) 56
BstF5I GGATG 3 cut(s) 12, 98, 136
BstKTI GATC 1 cut(s) 353
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 1 cut(s) 350
BstSCI CCNGG 1 cut(s) 371
BstSFI CTRYAG 1 cut(s) 243
BstV1I GCAGC 2 cut(s) 226, 229
Bsu15I ATCGAT 1 cut(s) 315
BsuTUI ATCGAT 1 cut(s) 315
BtsCI GGATG 3 cut(s) 12, 98, 136
BtsIMutI CAGTG 2 cut(s) 182, 208
BveI ACCTGC 1 cut(s) 236
ClaI ATCGAT 1 cut(s) 315
CseI GACGC 1 cut(s) 45
CspCI CAANNNNNGTGG 2 cut(s) 210, 245
CviAII CATG 2 cut(s) 51, 79
CviJI RGCY 3 cut(s) 169, 213, 255
CviKI_1 RGCY 3 cut(s) 169, 213, 255
DpnI GATC 1 cut(s) 352
DpnII GATC 1 cut(s) 350
Esp3I CGTCTC 1 cut(s) 63
FaeI CATG 2 cut(s) 54, 82
FaiI YATR 9 cut(s) 14, 42, 52, 80, 306, 355, 395, 401, 427
FatI CATG 2 cut(s) 50, 78
Fnu4HI GCNGC 2 cut(s) 240, 243
FokI GGATG 3 cut(s) 19, 85, 123
Fsp4HI GCNGC 2 cut(s) 240, 243
FspBI CTAG 1 cut(s) 440
GluI GCNGC 2 cut(s) 240, 243
GsaI CCCAGC 1 cut(s) 217
HapII CCGG 1 cut(s) 372
HgaI GACGC 1 cut(s) 45
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 2 cut(s) 54, 82
HinfI GANTC 4 cut(s) 20, 32, 218, 312
HpaII CCGG 1 cut(s) 372
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 2 cut(s) 232, 378
Hpy188I TCNGA 3 cut(s) 103, 149, 449
Hpy8I GTNNAC 2 cut(s) 232, 378
HpyAV CCTTC 1 cut(s) 157
HpyCH4III ACNGT 2 cut(s) 177, 382
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 1 cut(s) 245
HpySE526I ACGT 1 cut(s) 406
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 2 cut(s) 54, 82
Kzo9I GATC 1 cut(s) 350
LmnI GCTCC 2 cut(s) 166, 390
LpnPI CCDG 4 cut(s) 199, 231, 241, 385
Lsp1109I GCAGC 2 cut(s) 226, 229
LweI GCATC 1 cut(s) 107
MaeI CTAG 1 cut(s) 440
MaeII ACGT 1 cut(s) 406
MaeIII GTNAC 2 cut(s) 43, 132
MalI GATC 1 cut(s) 352
MboI GATC 1 cut(s) 350
MboII GAAGA 2 cut(s) 59, 317
MluCI AATT 3 cut(s) 301, 331, 358
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 2 cut(s) 10, 274
MseI TTAA 2 cut(s) 273, 419
MslI CAYNNNNRTG 2 cut(s) 201, 398
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 1 cut(s) 373
NciI CCSGG 1 cut(s) 373
NdeII GATC 1 cut(s) 350
NlaIII CATG 2 cut(s) 54, 82
OliI CACNNNNGTG 1 cut(s) 201
PfeI GAWTC 4 cut(s) 20, 32, 218, 312
PkrI GCNGC 2 cut(s) 241, 244
PspFI CCCAGC 1 cut(s) 213
PstI CTGCAG 1 cut(s) 247
RseI CAYNNNNRTG 2 cut(s) 201, 398
SaqAI TTAA 2 cut(s) 273, 419
SatI GCNGC 2 cut(s) 240, 243
Sau3AI GATC 1 cut(s) 350
ScrFI CCNGG 1 cut(s) 373
SetI ASST 3 cut(s) 171, 250, 409
SfaNI GCATC 1 cut(s) 107
SfcI CTRYAG 1 cut(s) 243
SmiMI CAYNNNNRTG 2 cut(s) 201, 398
Sse9I AATT 3 cut(s) 301, 331, 358
SspMI CTAG 1 cut(s) 440
StyD4I CCNGG 1 cut(s) 371
TaaI ACNGT 2 cut(s) 177, 382
TaiI ACGT 1 cut(s) 409
TaqI TCGA 1 cut(s) 315
TasI AATT 3 cut(s) 301, 331, 358
TfiI GAWTC 4 cut(s) 20, 32, 218, 312
Tru1I TTAA 2 cut(s) 273, 419
Tru9I TTAA 2 cut(s) 273, 419
TscAI CASTG 2 cut(s) 182, 208
TseI GCWGC 2 cut(s) 239, 242
TspDTI ATGAA 1 cut(s) 324
TspRI CASTG 2 cut(s) 182, 208
XspI CTAG 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.