RchiOBHm_Chr6g0264761

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser Thr consensus motif in nascent polypeptide chains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015438
Physical Location & Seq
Reverse (-)
20098467 .. 20105270
6804 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23746

Sequence Viewer

Length: 1107 bp
ATGCAGGCTGACATTGAGCTCCTAGTGTTTCAGGATACCGCACACTACCTCTCTCATGCAGTCAAGGCACAAACAATCAGTGTTAAATTGCCTGATGCAAGGATTGAATCTTATACGAGAGTAGACAATACAAAGACTGTTGGTTCAGAGATCAAATATGGTCCTTATGAGAATCTCCCTCCCTTTTCATACTCACCTATTGTTGTTCATTTTGAGAGCAATCAACCCTTTGCTGTTGCTGAAGAGTTGGTGCGAGAGATAGAGATTTCCCATTGGGGTAATGTTCAAGTGACAGAGCATTACAAATTTGTCCATGGAGGTGCCCAAAGCAAAGGAGAATTTTCGAGGCTTGATTATCAAGCCGGACCTCACGTGAGAGGTCAGTATGCCATTAGGCGTCTTGTTGCAAAGTTGCCACCAAGAGCTCATTCTGTCTATTATAGAGACGAAATTGGTAATATTTCAACATCTAACTTATGGAGTGATTCTAAGAAGACAGAACTAGAAATTGAGCCTCGGTATCCTGTGATTGGTGGTTGGAAAACTGCATTTACCATTGGATATGGTTTGCCACTTCATGACTTTTTATTTCAGTCTGATGGAAAACGCCTCATTGGCTTAACTTTTGGTTGCCCTATGAATGAGGTGGTCATCAACACTCTCATTGTGAAGGTTTTTCTGCCTGAGGGTTCTAGAGATATATCTACAAAGTTTTCCCACTTGGATATTGCTGGTAGGCCAGTGGTTGTACTGGAAAAGACTAATGCTGTGCCAGAGCATAATCAGAAATTCCAGGTCTATTACAAGTTCAACAGCCTTGCGATGCTTATGGAGCCTTTGATGTTGATTCCTGGATTTTTCTTCCTGTTTGTTGCCGGCATTGTCTGCATGCATGTAGACTTGTCCATCTCCAAATCTTCTGCTTCTTATCTGGCAAAACTACAGTGGGATGAAGTGCAAGCAACAATTCAGCAGATTCAACATATTATCAACCGAGGCCTCGTGATACATGATAAACTGGAAGCATCATTATGTGATCTTTCTCGGACAGGGGACATCCAAGCCTGTAAAGCAGCACGAAAAGCAGCTGATGGTTCGTTGAAATAG

Protein Analysis

368

Amino Acids

41.37

Weight (kDa)

6.93

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribophorin_I PF04597 2 - 295 1.2e-102 Ribophorin I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015438)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76400 AT1G76400
fragaria_vesca FvH4_5g25900
malus_domestica MD06G1005600.v1.1
prunus_persica Prupe.5G006900_v2.0.a1
pyrus_communis pycom06g00520
rosa_chinensis RchiOBHm_Chr6g0264761 RchiOBHm_Chr7g0216411
rosa_laevigata RLG00000002573
rosa_multiflora Rmu_sc0000692.1_g000009
rosa_roxburghii Rroxscaffold_3G00243260
rosa_rugosa Rorug07G0160300 Rorug07G0160300
rosa_samantha Rh7AG299200 Rh7DG300500
rosa_wichuraiana Rw7G025430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 320
AccI GTMKAC 2 cut(s) 123, 897
AciI CCGC 1 cut(s) 39
AcsI RAATTY 3 cut(s) 305, 338, 788
AcuI CTGAAG 1 cut(s) 261
AcvI CACGTG 1 cut(s) 373
AcyI GRCGYC 1 cut(s) 397
AfaI GTAC 1 cut(s) 750
AfiI CCNNNNNNNGG 1 cut(s) 530
AgsI TTSAA 6 cut(s) 107, 287, 465, 811, 980, 1102
AjnI CCWGG 2 cut(s) 792, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 412, 444
AluBI AGCT 3 cut(s) 19, 425, 1088
AluI AGCT 3 cut(s) 19, 425, 1088
Alw21I GWGCWC 2 cut(s) 21, 427
Alw26I GTCTC 1 cut(s) 438
AoxI GGCC 2 cut(s) 737, 997
ApeKI GCWGC 2 cut(s) 1073, 1085
ApoI RAATTY 3 cut(s) 305, 338, 788
AspS9I GGNCC 2 cut(s) 161, 365
AsuHPI GGTGA 1 cut(s) 186
AvaII GGWCC 2 cut(s) 161, 365
AxyI CCTNAGG 1 cut(s) 684
BaeGI GKGCMC 1 cut(s) 325
BanI GGYRCC 1 cut(s) 320
BanII GRGCYC 2 cut(s) 21, 427
BauI CACGAG 1 cut(s) 1001
BbrPI CACGTG 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 2 cut(s) 21, 427
BbvI GCAGC 2 cut(s) 1085, 1097
BccI CCATC 3 cut(s) 593, 914, 1085
BciT130I CCWGG 2 cut(s) 794, 852
BciVI GTATCC 2 cut(s) 28, 531
BcoDI GTCTC 1 cut(s) 438
BfaI CTAG 3 cut(s) 23, 503, 693
BfmI CTRYAG 1 cut(s) 941
BfuI GTATCC 2 cut(s) 28, 531
BglI GCCNNNNNGGC 1 cut(s) 615
BisI GCNGC 2 cut(s) 1074, 1086
BlsI GCNGC 2 cut(s) 1075, 1087
Bme1390I CCNGG 2 cut(s) 794, 852
Bme18I GGWCC 2 cut(s) 161, 365
BmgT120I GGNCC 2 cut(s) 161, 365
BmiI GGNNCC 2 cut(s) 322, 834
BmrFI CCNGG 2 cut(s) 794, 852
BmsI GCATC 3 cut(s) 85, 813, 1034
BpiI GAAGAC 1 cut(s) 500
BsaAI YACGTR 1 cut(s) 373
BsaHI GRCGYC 1 cut(s) 397
BsaJI CCNNGG 3 cut(s) 313, 515, 994
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse118I RCCGGY 1 cut(s) 875
Bse1I ACTGG 3 cut(s) 740, 756, 1023
Bse21I CCTNAGG 1 cut(s) 684
BseBI CCWGG 2 cut(s) 794, 852
BseDI CCNNGG 3 cut(s) 313, 515, 994
BseGI GGATG 2 cut(s) 955, 1056
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMII CTCAG 1 cut(s) 675
BseNI ACTGG 3 cut(s) 740, 756, 1023
BseSI GKGCMC 1 cut(s) 325
BseXI GCAGC 2 cut(s) 1085, 1097
BshFI GGCC 2 cut(s) 739, 999
BshNI GGYRCC 1 cut(s) 320
BsiHKAI GWGCWC 2 cut(s) 21, 427
BsiSI CCGG 2 cut(s) 363, 876
BslFI GGGAC 1 cut(s) 1067
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 1 cut(s) 438
BsmBI CGTCTC 1 cut(s) 438
BsmFI GGGAC 1 cut(s) 1067
BsnI GGCC 2 cut(s) 739, 999
Bsp1286I GDGCHC 3 cut(s) 21, 325, 427
Bsp143I GATC 2 cut(s) 150, 1036
Bsp19I CCATGG 1 cut(s) 313
BspACI CCGC 1 cut(s) 39
BspANI GGCC 2 cut(s) 739, 999
BspCNI CTCAG 1 cut(s) 676
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 2 cut(s) 322, 834
BspT107I GGYRCC 1 cut(s) 320
BsrFI RCCGGY 1 cut(s) 875
BsrI ACTGG 3 cut(s) 740, 756, 1023
BssAI RCCGGY 1 cut(s) 875
BssECI CCNNGG 3 cut(s) 313, 515, 994
BssMI GATC 2 cut(s) 150, 1036
BssNI GRCGYC 1 cut(s) 397
BssSI CACGAG 1 cut(s) 1001
BssT1I CCWWGG 1 cut(s) 313
Bst2BI CACGAG 1 cut(s) 1001
Bst2UI CCWGG 2 cut(s) 794, 852
Bst4CI ACNGT 2 cut(s) 139, 945
Bst6I CTCTTC 1 cut(s) 237
BstACI GRCGYC 1 cut(s) 397
BstAPI GCANNNNNTGC 1 cut(s) 885
BstBAI YACGTR 1 cut(s) 373
BstC8I GCNNGC 4 cut(s) 6, 877, 890, 960
BstDEI CTNAG 2 cut(s) 489, 684
BstDSI CCRYGG 1 cut(s) 313
BstF5I GGATG 2 cut(s) 955, 1056
BstKTI GATC 2 cut(s) 153, 1039
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 2 cut(s) 150, 1036
BstMWI GCNNNNNNNGC 6 cut(s) 65, 615, 832, 885, 1070, 1082
BstNI CCWGG 2 cut(s) 794, 852
BstNSI RCATGY 2 cut(s) 892, 896
BstSCI CCNGG 2 cut(s) 792, 850
BstSFI CTRYAG 1 cut(s) 941
BstSLI GKGCMC 1 cut(s) 325
BstV1I GCAGC 2 cut(s) 1085, 1097
BstV2I GAAGAC 1 cut(s) 500
Bsu36I CCTNAGG 1 cut(s) 684
BsuI GTATCC 2 cut(s) 28, 531
BsuRI GGCC 2 cut(s) 739, 999
BtgI CCRYGG 1 cut(s) 313
BtgZI GCGATG 1 cut(s) 836
BtsCI GGATG 2 cut(s) 955, 1056
BtsIMutI CAGTG 3 cut(s) 85, 747, 950
Cac8I GCNNGC 4 cut(s) 6, 877, 890, 960
CciI TCATGA 1 cut(s) 577
Cfr10I RCCGGY 1 cut(s) 875
Cfr13I GGNCC 2 cut(s) 161, 365
CseI GACGC 1 cut(s) 386
Csp6I GTAC 1 cut(s) 749
CviAII CATG 6 cut(s) 56, 314, 578, 889, 893, 1010
CviQI GTAC 1 cut(s) 749
DdeI CTNAG 2 cut(s) 489, 684
DpnI GATC 2 cut(s) 152, 1038
DpnII GATC 2 cut(s) 150, 1036
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Ecl136II GAGCTC 2 cut(s) 19, 425
Eco130I CCWWGG 1 cut(s) 313
Eco147I AGGCCT 1 cut(s) 999
Eco24I GRGCYC 2 cut(s) 21, 427
Eco47I GGWCC 2 cut(s) 161, 365
Eco53kI GAGCTC 2 cut(s) 19, 425
Eco57I CTGAAG 1 cut(s) 261
Eco72I CACGTG 1 cut(s) 373
Eco81I CCTNAGG 1 cut(s) 684
EcoICRI GAGCTC 2 cut(s) 19, 425
EcoRII CCWGG 2 cut(s) 792, 850
EcoT14I CCWWGG 1 cut(s) 313
EcoT22I ATGCAT 1 cut(s) 894
EcoT38I GRGCYC 2 cut(s) 21, 427
ErhI CCWWGG 1 cut(s) 313
Esp3I CGTCTC 1 cut(s) 438
FaeI CATG 6 cut(s) 59, 317, 581, 892, 896, 1013
FaqI GGGAC 1 cut(s) 1067
FatI CATG 6 cut(s) 55, 313, 577, 888, 892, 1009
FblI GTMKAC 2 cut(s) 123, 897
Fnu4HI GCNGC 2 cut(s) 1074, 1086
FokI GGATG 2 cut(s) 962, 1043
FriOI GRGCYC 2 cut(s) 21, 427
Fsp4HI GCNGC 2 cut(s) 1074, 1086
FspBI CTAG 3 cut(s) 23, 503, 693
GluI GCNGC 2 cut(s) 1074, 1086
HaeIII GGCC 2 cut(s) 739, 999
HapII CCGG 2 cut(s) 363, 876
HgaI GACGC 1 cut(s) 386
Hin1I GRCGYC 1 cut(s) 397
Hin1II CATG 6 cut(s) 59, 317, 581, 892, 896, 1013
HinfI GANTC 5 cut(s) 107, 172, 485, 847, 976
HpaII CCGG 2 cut(s) 363, 876
HphI GGTGA 1 cut(s) 186
Hpy166II GTNNAC 2 cut(s) 124, 898
Hpy188I TCNGA 4 cut(s) 148, 598, 786, 1047
Hpy188III TCNNGA 4 cut(s) 32, 578, 693, 1003
Hpy8I GTNNAC 2 cut(s) 124, 898
HpyAV CCTTC 1 cut(s) 664
HpyCH4III ACNGT 2 cut(s) 139, 945
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 8 cut(s) 4, 59, 98, 407, 548, 888, 892, 958
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 615, 832, 885, 1070, 1082
HpyF3I CTNAG 2 cut(s) 489, 684
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 397
Hsp92II CATG 6 cut(s) 59, 317, 581, 892, 896, 1013
KroI GCCGGC 1 cut(s) 875
KroNI GCCGGC 1 cut(s) 877
Kzo9I GATC 2 cut(s) 150, 1036
LmnI GCTCC 2 cut(s) 24, 832
Lsp1109I GCAGC 2 cut(s) 1085, 1097
LweI GCATC 3 cut(s) 85, 813, 1034
MaeI CTAG 3 cut(s) 23, 503, 693
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 289
MalI GATC 2 cut(s) 152, 1038
MboI GATC 2 cut(s) 150, 1036
MboII GAAGA 4 cut(s) 254, 505, 853, 909
MhlI GDGCHC 3 cut(s) 21, 325, 427
MluCI AATT 7 cut(s) 86, 305, 338, 450, 507, 788, 966
MmeI TCCRAC 1 cut(s) 518
Mph1103I ATGCAT 1 cut(s) 894
MroNI GCCGGC 1 cut(s) 875
MseI TTAA 2 cut(s) 84, 620
MslI CAYNNNNRTG 2 cut(s) 318, 1030
MspA1I CMGCKG 1 cut(s) 1088
MspI CCGG 2 cut(s) 363, 876
MspR9I CCNGG 2 cut(s) 794, 852
MvaI CCWGG 2 cut(s) 794, 852
MwoI GCNNNNNNNGC 6 cut(s) 65, 615, 832, 885, 1070, 1082
NaeI GCCGGC 1 cut(s) 877
NcoI CCATGG 1 cut(s) 313
NdeII GATC 2 cut(s) 150, 1036
NgoMIV GCCGGC 1 cut(s) 875
NlaIII CATG 6 cut(s) 59, 317, 581, 892, 896, 1013
NlaIV GGNNCC 2 cut(s) 322, 834
NmuCI GTSAC 1 cut(s) 289
NsiI ATGCAT 1 cut(s) 894
NspI RCATGY 2 cut(s) 892, 896
PaeI GCATGC 1 cut(s) 892
PagI TCATGA 1 cut(s) 577
PceI AGGCCT 1 cut(s) 999
PdiI GCCGGC 1 cut(s) 877
PfeI GAWTC 5 cut(s) 107, 172, 485, 847, 976
PfoI TCCNGGA 1 cut(s) 850
PkrI GCNGC 2 cut(s) 1075, 1087
PmaCI CACGTG 1 cut(s) 373
PmlI CACGTG 1 cut(s) 373
Ppu21I YACGTR 1 cut(s) 373
Psp124BI GAGCTC 2 cut(s) 21, 427
Psp6I CCWGG 2 cut(s) 792, 850
PspCI CACGTG 1 cut(s) 373
PspGI CCWGG 2 cut(s) 792, 850
PspN4I GGNNCC 2 cut(s) 322, 834
PspPI GGNCC 2 cut(s) 161, 365
PvuII CAGCTG 1 cut(s) 1088
RsaI GTAC 1 cut(s) 750
RsaNI GTAC 1 cut(s) 749
RseI CAYNNNNRTG 2 cut(s) 318, 1030
SacI GAGCTC 2 cut(s) 21, 427
SaqAI TTAA 2 cut(s) 84, 620
SatI GCNGC 2 cut(s) 1074, 1086
Sau3AI GATC 2 cut(s) 150, 1036
Sau96I GGNCC 2 cut(s) 161, 365
ScrFI CCNGG 2 cut(s) 794, 852
SduI GDGCHC 3 cut(s) 21, 325, 427
SfaNI GCATC 3 cut(s) 85, 813, 1034
SfcI CTRYAG 1 cut(s) 941
SinI GGWCC 2 cut(s) 161, 365
SmiMI CAYNNNNRTG 2 cut(s) 318, 1030
SphI GCATGC 1 cut(s) 892
Sse9I AATT 7 cut(s) 86, 305, 338, 450, 507, 788, 966
SseBI AGGCCT 1 cut(s) 999
SsiI CCGC 1 cut(s) 39
SspI AATATT 1 cut(s) 460
SspMI CTAG 3 cut(s) 23, 503, 693
SstI GAGCTC 2 cut(s) 21, 427
StuI AGGCCT 1 cut(s) 999
StyD4I CCNGG 2 cut(s) 792, 850
StyI CCWWGG 1 cut(s) 313
TaaI ACNGT 2 cut(s) 139, 945
TaiI ACGT 1 cut(s) 375
TaqI TCGA 1 cut(s) 344
TasI AATT 7 cut(s) 86, 305, 338, 450, 507, 788, 966
TatI WGTACW 1 cut(s) 748
TfiI GAWTC 5 cut(s) 107, 172, 485, 847, 976
Tru1I TTAA 2 cut(s) 84, 620
Tru9I TTAA 2 cut(s) 84, 620
TscAI CASTG 3 cut(s) 85, 747, 950
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 2 cut(s) 1073, 1085
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 5 cut(s) 177, 197, 566, 653, 966
TspRI CASTG 3 cut(s) 85, 747, 950
VpaK11BI GGWCC 2 cut(s) 161, 365
XapI RAATTY 3 cut(s) 305, 338, 788
XbaI TCTAGA 1 cut(s) 692
XceI RCATGY 2 cut(s) 892, 896
XmiI GTMKAC 2 cut(s) 123, 897
XspI CTAG 3 cut(s) 23, 503, 693
Zsp2I ATGCAT 1 cut(s) 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.