RchiOBHm_Chr6g0309041

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0021271
Physical Location & Seq
Reverse (-)
66974249 .. 66975254
1006 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27789

Sequence Viewer

Length: 807 bp
ATGAAGTACATCCGCCTCATTATTATAACAGACTTCACCATACCCATCTCCATGGAAACTTCAACCTACTACTTCATCTTGTTCTTCATCATCTTTCTTTTTCTTAAGAACAATTTGCAGAGAATTAACAAAAGATTCCCACCAAGCCCCGGTCTCTGTCTCCCCATAAAAAAACCTCTATATAGAACACTTGCAGAGTTGTCCGACAAATATGGTCCAATTCTTTACGTTAGATATGGCTCACGGCCTGTTATTCTTGTATCCTCCCCTTCTGCAGCTGAGGAATGCTTCACCAAGCACGACGTCGCTTTTGCAAATCGTCCAAAGCTGCTTGCTGGCAAATACCTTGGCTATAACTATACCACTATTAGCTGGGCTTCTTATGGCTCTCACTGGCACAACTTGAGACGCATAGCTTCCGTTAAATTATTGTCATCCAACAGCCTTCAGATGTTTCATGGTATATGTGCAGATGAAATTTGGGAGGAAGCTAAAAGGTTTCAACAAATTGTAGCAGAGTCAATTCAATTGACTGGAGTTACCAACATTGGAGATTTCATACCAGTTTTGAACTATGTGGGAGTAAGTAGACTTGAGAAGAAGTTGGTGATATTGCAGAAGAAGAAGGATAAGTTCCTGCAAGATTTGATCAATCAACATAGACAAAAGCAGAGTGACTCTGCTTCTGAACAGATGAGCAAGACAATGGTGGATGTCTTGTTGTCACTGCAAGGAACTGAACCTGAATACCATACCGATGAAATCATAAGGGGCATGATGCATGTAAGCTATCTAACCACACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

268

Amino Acids

30.93

Weight (kDa)

9.41

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 57 - 155 1.6e-10 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021271)

Species Orthologous Gene IDs
pyrus_communis pycom08g07300
rosa_chinensis RchiOBHm_Chr6g0309041
rosa_rugosa Rorug06G0369300
rosa_samantha Rh6BG490700 Rh6DG482800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 26
AatII GACGTC 1 cut(s) 306
AccI GTMKAC 1 cut(s) 589
AciI CCGC 1 cut(s) 13
AcsI RAATTY 1 cut(s) 477
AcuI CTGAAG 1 cut(s) 431
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 1 cut(s) 149
AflII CTTAAG 1 cut(s) 104
AgsI TTSAA 4 cut(s) 63, 503, 527, 571
AluBI AGCT 6 cut(s) 278, 328, 372, 416, 491, 789
AluI AGCT 6 cut(s) 278, 328, 372, 416, 491, 789
Alw26I GTCTC 3 cut(s) 158, 164, 400
AoxI GGCC 1 cut(s) 245
ApeKI GCWGC 2 cut(s) 275, 328
ApoI RAATTY 1 cut(s) 477
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
AspS9I GGNCC 1 cut(s) 215
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 3 cut(s) 28, 283, 619
AvaII GGWCC 1 cut(s) 215
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 287, 315
BccI CCATC 1 cut(s) 53
BceAI ACGGC 1 cut(s) 260
BciVI GTATCC 1 cut(s) 271
BclI TGATCA 1 cut(s) 648
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 3 cut(s) 158, 164, 400
BfmI CTRYAG 1 cut(s) 273
BfrI CTTAAG 1 cut(s) 104
BfuI GTATCC 1 cut(s) 271
BisI GCNGC 2 cut(s) 276, 329
BlsI GCNGC 2 cut(s) 277, 330
Bme1390I CCNGG 1 cut(s) 150
Bme18I GGWCC 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 215
BmrFI CCNGG 1 cut(s) 150
BmsI GCATC 1 cut(s) 768
BpmI CTGGAG 1 cut(s) 555
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 2 cut(s) 424, 614
BpuMI CCSGG 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 3 cut(s) 51, 148, 346
Bsc4I CCNNNNNNNGG 1 cut(s) 149
Bse1I ACTGG 3 cut(s) 398, 538, 563
BseDI CCNNGG 3 cut(s) 51, 148, 346
BseGI GGATG 3 cut(s) 9, 434, 718
BseLI CCNNNNNNNGG 1 cut(s) 149
BseMII CTCAG 1 cut(s) 270
BseNI ACTGG 3 cut(s) 398, 538, 563
BseXI GCAGC 2 cut(s) 287, 315
BseYI CCCAGC 1 cut(s) 372
BsgI GTGCAG 1 cut(s) 489
BshFI GGCC 1 cut(s) 247
BsiSI CCGG 1 cut(s) 150
BslI CCNNNNNNNGG 1 cut(s) 149
BsmAI GTCTC 3 cut(s) 158, 164, 400
BsmBI CGTCTC 1 cut(s) 400
BsmI GAATGC 1 cut(s) 290
BsnI GGCC 1 cut(s) 247
Bso31I GGTCTC 1 cut(s) 158
Bsp143I GATC 1 cut(s) 648
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 1 cut(s) 13
BspANI GGCC 1 cut(s) 247
BspCNI CTCAG 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 277
BspTI CTTAAG 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 158
BsrI ACTGG 3 cut(s) 398, 538, 563
BssECI CCNNGG 3 cut(s) 51, 148, 346
BssMI GATC 1 cut(s) 648
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 2 cut(s) 51, 346
BstACI GRCGYC 1 cut(s) 303
BstAFI CTTAAG 1 cut(s) 104
BstC8I GCNNGC 2 cut(s) 333, 337
BstDEI CTNAG 1 cut(s) 279
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 3 cut(s) 9, 434, 718
BstKTI GATC 1 cut(s) 651
BstMAI GTCTC 3 cut(s) 158, 164, 400
BstMBI GATC 1 cut(s) 648
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 1 cut(s) 148
BstSFI CTRYAG 1 cut(s) 273
BstV1I GCAGC 2 cut(s) 287, 315
BstXI CCANNNNNNTGG 1 cut(s) 52
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 1 cut(s) 247
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 3 cut(s) 9, 434, 718
BtsI GCAGTG 1 cut(s) 725
BtsIMutI CAGTG 2 cut(s) 391, 725
Cac8I GCNNGC 2 cut(s) 333, 337
Cfr13I GGNCC 1 cut(s) 215
CseI GACGC 1 cut(s) 417
Csp6I GTAC 1 cut(s) 7
CviAII CATG 4 cut(s) 52, 458, 775, 782
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 1 cut(s) 279
DpnI GATC 1 cut(s) 650
DpnII GATC 1 cut(s) 648
Eco130I CCWWGG 2 cut(s) 51, 346
Eco31I GGTCTC 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 431
EcoT14I CCWWGG 2 cut(s) 51, 346
EcoT22I ATGCAT 1 cut(s) 783
ErhI CCWWGG 2 cut(s) 51, 346
Esp3I CGTCTC 1 cut(s) 400
FaeI CATG 4 cut(s) 55, 461, 778, 785
FatI CATG 4 cut(s) 51, 457, 774, 781
FbaI TGATCA 1 cut(s) 648
FblI GTMKAC 1 cut(s) 589
Fnu4HI GCNGC 2 cut(s) 276, 329
FokI GGATG 2 cut(s) 421, 725
Fsp4HI GCNGC 2 cut(s) 276, 329
GluI GCNGC 2 cut(s) 276, 329
GsaI CCCAGC 1 cut(s) 376
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 1 cut(s) 247
HapII CCGG 1 cut(s) 150
HgaI GACGC 1 cut(s) 417
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 4 cut(s) 55, 461, 778, 785
HinfI GANTC 3 cut(s) 135, 518, 677
HpaII CCGG 1 cut(s) 150
HphI GGTGA 3 cut(s) 28, 283, 619
Hpy166II GTNNAC 1 cut(s) 590
Hpy188I TCNGA 3 cut(s) 205, 450, 688
Hpy8I GTNNAC 1 cut(s) 590
Hpy99I CGWCG 2 cut(s) 305, 308
HpyAV CCTTC 3 cut(s) 279, 455, 619
HpyCH4IV ACGT 2 cut(s) 228, 303
HpyCH4V TGCA 9 cut(s) 118, 194, 275, 314, 470, 616, 640, 730, 781
HpyF3I CTNAG 1 cut(s) 279
HpySE526I ACGT 2 cut(s) 228, 303
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 4 cut(s) 55, 461, 778, 785
Ksp22I TGATCA 1 cut(s) 648
Kzo9I GATC 1 cut(s) 648
LpnPI CCDG 9 cut(s) 163, 261, 321, 358, 379, 519, 576, 650, 756
Lsp1109I GCAGC 2 cut(s) 287, 315
LweI GCATC 1 cut(s) 768
MaeII ACGT 2 cut(s) 228, 303
MaeIII GTNAC 3 cut(s) 538, 674, 723
MalI GATC 1 cut(s) 650
MboI GATC 1 cut(s) 648
MboII GAAGA 4 cut(s) 76, 610, 631, 634
MfeI CAATTG 1 cut(s) 527
MluCI AATT 8 cut(s) 112, 123, 219, 425, 477, 507, 522, 527
MlyI GAGTC 2 cut(s) 527, 671
MmeI TCCRAC 2 cut(s) 228, 462
MnlI CCTC 5 cut(s) 26, 186, 274, 274, 478
Mph1103I ATGCAT 1 cut(s) 783
MseI TTAA 3 cut(s) 105, 126, 423
MslI CAYNNNNRTG 2 cut(s) 50, 756
MspA1I CMGCKG 1 cut(s) 278
MspCI CTTAAG 1 cut(s) 104
MspI CCGG 1 cut(s) 150
MspR9I CCNGG 1 cut(s) 150
MunI CAATTG 1 cut(s) 527
Mva1269I GAATGC 1 cut(s) 290
NciI CCSGG 1 cut(s) 150
NcoI CCATGG 1 cut(s) 51
NdeII GATC 1 cut(s) 648
NlaIII CATG 4 cut(s) 55, 461, 778, 785
NmuCI GTSAC 2 cut(s) 674, 723
NsiI ATGCAT 1 cut(s) 783
NspI RCATGY 1 cut(s) 785
PctI GAATGC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 135
PkrI GCNGC 2 cut(s) 277, 330
PleI GAGTC 2 cut(s) 526, 671
PpsI GAGTC 2 cut(s) 526, 671
PsiI TTATAA 1 cut(s) 26
PspFI CCCAGC 1 cut(s) 372
PspPI GGNCC 1 cut(s) 215
PstI CTGCAG 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 278
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
RseI CAYNNNNRTG 2 cut(s) 50, 756
SaqAI TTAA 3 cut(s) 105, 126, 423
SatI GCNGC 2 cut(s) 276, 329
Sau3AI GATC 1 cut(s) 648
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 2 cut(s) 527, 671
ScrFI CCNGG 1 cut(s) 150
SfaNI GCATC 1 cut(s) 768
SfcI CTRYAG 1 cut(s) 273
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 2 cut(s) 50, 756
SmlI CTYRAG 3 cut(s) 104, 403, 593
SmoI CTYRAG 3 cut(s) 104, 403, 593
Sse9I AATT 8 cut(s) 112, 123, 219, 425, 477, 507, 522, 527
SsiI CCGC 1 cut(s) 13
StyD4I CCNGG 1 cut(s) 148
StyI CCWWGG 2 cut(s) 51, 346
TaiI ACGT 2 cut(s) 231, 306
TasI AATT 8 cut(s) 112, 123, 219, 425, 477, 507, 522, 527
TatI WGTACW 1 cut(s) 6
TfiI GAWTC 1 cut(s) 135
Tru1I TTAA 3 cut(s) 105, 126, 423
Tru9I TTAA 3 cut(s) 105, 126, 423
TscAI CASTG 2 cut(s) 398, 732
TseFI GTSAC 2 cut(s) 674, 723
TseI GCWGC 2 cut(s) 275, 328
Tsp45I GTSAC 2 cut(s) 674, 723
TspDTI ATGAA 7 cut(s) 17, 64, 76, 446, 489, 547, 774
TspGWI ACGGA 1 cut(s) 409
TspRI CASTG 2 cut(s) 398, 732
Vha464I CTTAAG 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 1 cut(s) 477
XceI RCATGY 1 cut(s) 785
XmiI GTMKAC 1 cut(s) 589
ZraI GACGTC 1 cut(s) 304
Zsp2I ATGCAT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.