Rh6BG490700

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0021271
Physical Location & Seq
Reverse (-)
69436411 .. 69438643
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG490700.1

Sequence Viewer

Length: 984 bp
ATGGAAACTTCAACCTACTCCTACATCTTGCTCTTCATCATCTTTCTTTTCCTCAAGAGTTATTTGCAGAAAAATAAGAAGAGATTGCCACCAAGCCCTGCTCTCTCTCTCCCCATAATAGGCCATCTCTATCTTTTTAAGAAACCCCTCCACAGAACACTTACAAAGTTATCCGACAAATATGGTCCAATTGTATACATCAAATTTGGCTCCCGCCCTGTCATTCTTGTATCCTCCCCTTCTGCAGCTGAAGAATGCTTCACCAAGCACGATGTCGCTTTTGCAAACCGTCCAAAGCTGCTTGCCGGCAAACACCTTGGGTATAACTATACCACAGTTACCTGGGCTTCTTATGGCTCCCACTGGCGCAACTTGAGACGCATAGCTTCCCTTGAACTATTGTCATCCAACAGCCTTCAGATGTTTCATGGCATACGTGCAGATGAAATCAGGTCACTCGTTTGCCAGCTTTTTCGAGGCTGTAATGGTGGTGAGTTTCAGGGTGTAGAGATGAAGTCAAAGTTTTTTCAGCTAACTCTCAATGTTATAATGAGAATGGTTGCTGGGAAAAGATACTATGGGGAAGATATAGCAGATTTGGAGGAAGCTAAAATGTTTCAACAAATTGTTACAGAGGGGTTTCAATTGACTGGGGTTACTAACGTTGGAGATTTCATACCAGTTTTAAACTACGTAGGAGTAAGTAAGCTTGAGAAGAAGTTGATAATATTGCATAAGAAGAGGGATAAGTTCCTGCAAGATTTGATTGATCAACATAGAAAAATGCAGAGCGACTCTGCTTTGGAAAAGAGAAGCAAGACCATGGTGGATGTCATGTTGTCACTGCAAGGAACTGAAGCCGAATACTATACTGATGAAATCATAAGGGGCATGATGCATAAAGGCTCAGACCCACAAGCTGCTAGCATCCAGTTACATAAACATCATGTCCTTCAAGCCAACATCGCATTCTCCAAGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00905 ko00943 ko01100 ko01110 ko04913 ko04917 ko04927 ko04934 map00140 map00905 map00943 map01100 map01110 map04913 map04917 map04927 map04934
Pfam Domains
Protein Families
p450

Protein Analysis

327

Amino Acids

37.33

Weight (kDa)

9.58

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 31 - 295 1.5e-28 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021271)

Species Orthologous Gene IDs
pyrus_communis pycom08g07300
rosa_chinensis RchiOBHm_Chr6g0309041
rosa_rugosa Rorug06G0369300
rosa_samantha Rh6BG490700 Rh6DG482800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 548
AccI GTMKAC 1 cut(s) 195
AciI CCGC 1 cut(s) 214
AclI AACGTT 1 cut(s) 663
AcsI RAATTY 1 cut(s) 203
AcuI CTGAAG 3 cut(s) 270, 401, 876
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 5 cut(s) 12, 395, 620, 644, 956
AjnI CCWGG 1 cut(s) 341
AjuI GAANNNNNNNTTGG 1 cut(s) 953
AluBI AGCT 8 cut(s) 248, 298, 386, 469, 532, 608, 709, 920
AluI AGCT 8 cut(s) 248, 298, 386, 469, 532, 608, 709, 920
Alw26I GTCTC 1 cut(s) 370
AoxI GGCC 1 cut(s) 121
ApeKI GCWGC 3 cut(s) 245, 298, 920
ApoI RAATTY 1 cut(s) 203
AspLEI GCGC 1 cut(s) 369
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 253, 503
AsuNHI GCTAGC 1 cut(s) 923
AvaII GGWCC 1 cut(s) 185
BbvI GCAGC 3 cut(s) 257, 285, 907
BccI CCATC 1 cut(s) 132
BciT130I CCWGG 1 cut(s) 343
BciVI GTATCC 1 cut(s) 241
BclI TGATCA 1 cut(s) 769
BcoDI GTCTC 1 cut(s) 370
BfaI CTAG 1 cut(s) 924
BfmI CTRYAG 1 cut(s) 243
BfuI GTATCC 1 cut(s) 241
BisI GCNGC 3 cut(s) 246, 299, 921
BlsI GCNGC 3 cut(s) 247, 300, 922
Bme1390I CCNGG 1 cut(s) 343
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 2 cut(s) 211, 358
BmrFI CCNGG 1 cut(s) 343
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 2 cut(s) 885, 936
BmtI GCTAGC 1 cut(s) 927
BmuI ACTGGG 1 cut(s) 660
BpuEI CTTGAG 3 cut(s) 38, 394, 731
BsaAI YACGTR 2 cut(s) 437, 694
BsaJI CCNNGG 3 cut(s) 316, 342, 822
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 4 cut(s) 368, 655, 680, 931
BseBI CCWGG 1 cut(s) 343
BseDI CCNNGG 3 cut(s) 316, 342, 822
BseGI GGATG 3 cut(s) 404, 835, 927
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMII CTCAG 1 cut(s) 921
BseNI ACTGG 4 cut(s) 368, 655, 680, 931
BseXI GCAGC 3 cut(s) 257, 285, 907
BseYI CCCAGC 1 cut(s) 563
BsgI GTGCAG 1 cut(s) 459
BshFI GGCC 1 cut(s) 123
BsiSI CCGG 1 cut(s) 306
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 1 cut(s) 370
BsmBI CGTCTC 1 cut(s) 370
BsmI GAATGC 2 cut(s) 260, 968
BsnI GGCC 1 cut(s) 123
Bsp143I GATC 1 cut(s) 769
Bsp19I CCATGG 1 cut(s) 822
BspACI CCGC 1 cut(s) 214
BspANI GGCC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 920
BspLI GGNNCC 2 cut(s) 211, 358
BspMAI CTGCAG 1 cut(s) 247
BspOI GCTAGC 1 cut(s) 927
BspQI GCTCTTC 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 4 cut(s) 368, 655, 680, 931
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 3 cut(s) 316, 342, 822
BssMI GATC 1 cut(s) 769
BssNAI GTATAC 1 cut(s) 196
BssT1I CCWWGG 2 cut(s) 316, 822
Bst1107I GTATAC 1 cut(s) 196
Bst2UI CCWGG 1 cut(s) 343
Bst4CI ACNGT 2 cut(s) 290, 337
Bst6I CTCTTC 3 cut(s) 38, 74, 734
BstBAI YACGTR 2 cut(s) 437, 694
BstC8I GCNNGC 4 cut(s) 303, 307, 467, 925
BstDEI CTNAG 1 cut(s) 907
BstDSI CCRYGG 1 cut(s) 822
BstF5I GGATG 3 cut(s) 404, 835, 927
BstHHI GCGC 1 cut(s) 369
BstKTI GATC 1 cut(s) 772
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 1 cut(s) 769
BstMWI GCNNNNNNNGC 1 cut(s) 965
BstNI CCWGG 1 cut(s) 343
BstSCI CCNGG 1 cut(s) 341
BstSFI CTRYAG 1 cut(s) 243
BstSNI TACGTA 1 cut(s) 694
BstV1I GCAGC 3 cut(s) 257, 285, 907
BstZ17I GTATAC 1 cut(s) 196
BsuI GTATCC 1 cut(s) 241
BsuRI GGCC 1 cut(s) 123
BtgI CCRYGG 1 cut(s) 822
BtgZI GCGATG 1 cut(s) 949
BtsCI GGATG 3 cut(s) 404, 835, 927
BtsI GCAGTG 1 cut(s) 842
BtsIMutI CAGTG 2 cut(s) 361, 842
Cac8I GCNNGC 4 cut(s) 303, 307, 467, 925
CfoI GCGC 1 cut(s) 369
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 1 cut(s) 185
CseI GACGC 1 cut(s) 387
CviAII CATG 5 cut(s) 428, 823, 835, 892, 947
DdeI CTNAG 1 cut(s) 907
DpnI GATC 1 cut(s) 771
DpnII GATC 1 cut(s) 769
DraI TTTAAA 1 cut(s) 687
Eam1104I CTCTTC 3 cut(s) 38, 74, 734
EarI CTCTTC 3 cut(s) 38, 74, 734
Eco105I TACGTA 1 cut(s) 694
Eco130I CCWWGG 2 cut(s) 316, 822
Eco47I GGWCC 1 cut(s) 185
Eco57I CTGAAG 3 cut(s) 270, 401, 876
EcoRII CCWGG 1 cut(s) 341
EcoT14I CCWWGG 2 cut(s) 316, 822
EcoT22I ATGCAT 1 cut(s) 900
ErhI CCWWGG 2 cut(s) 316, 822
Esp3I CGTCTC 1 cut(s) 370
FaeI CATG 5 cut(s) 431, 826, 838, 895, 950
FatI CATG 5 cut(s) 427, 822, 834, 891, 946
FauI CCCGC 1 cut(s) 221
FbaI TGATCA 1 cut(s) 769
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 3 cut(s) 246, 299, 921
FokI GGATG 3 cut(s) 391, 842, 914
Fsp4HI GCNGC 3 cut(s) 246, 299, 921
FspBI CTAG 1 cut(s) 924
GlaI GCGC 1 cut(s) 368
GluI GCNGC 3 cut(s) 246, 299, 921
GsaI CCCAGC 1 cut(s) 567
HaeIII GGCC 1 cut(s) 123
HapII CCGG 1 cut(s) 306
HgaI GACGC 1 cut(s) 387
HhaI GCGC 1 cut(s) 369
Hin1II CATG 5 cut(s) 431, 826, 838, 895, 950
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 1 cut(s) 794
HpaII CCGG 1 cut(s) 306
HphI GGTGA 2 cut(s) 253, 503
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 3 cut(s) 175, 420, 910
Hpy188III TCNNGA 2 cut(s) 55, 981
Hpy8I GTNNAC 1 cut(s) 196
HpyAV CCTTC 3 cut(s) 249, 425, 962
HpyCH4III ACNGT 2 cut(s) 290, 337
HpyCH4IV ACGT 3 cut(s) 436, 663, 693
HpyCH4V TGCA 9 cut(s) 67, 245, 284, 440, 733, 757, 787, 847, 898
HpyF10VI GCNNNNNNNGC 1 cut(s) 965
HpyF3I CTNAG 1 cut(s) 907
HpySE526I ACGT 3 cut(s) 436, 663, 693
Hsp92II CATG 5 cut(s) 431, 826, 838, 895, 950
HspAI GCGC 1 cut(s) 367
KroI GCCGGC 1 cut(s) 305
KroNI GCCGGC 1 cut(s) 307
Ksp22I TGATCA 1 cut(s) 769
Kzo9I GATC 1 cut(s) 769
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 2 cut(s) 215, 362
Lsp1109I GCAGC 3 cut(s) 257, 285, 907
LweI GCATC 2 cut(s) 885, 936
MaeI CTAG 1 cut(s) 924
MaeII ACGT 3 cut(s) 436, 663, 693
MaeIII GTNAC 6 cut(s) 337, 453, 628, 655, 840, 933
MalI GATC 1 cut(s) 771
MboI GATC 1 cut(s) 769
MboII GAAGA 6 cut(s) 25, 91, 263, 596, 727, 751
MfeI CAATTG 2 cut(s) 189, 644
MluCI AATT 4 cut(s) 189, 203, 624, 644
MlyI GAGTC 1 cut(s) 788
MmeI TCCRAC 3 cut(s) 198, 432, 646
MnlI CCTC 7 cut(s) 62, 158, 244, 470, 595, 628, 735
Mph1103I ATGCAT 1 cut(s) 900
MroNI GCCGGC 1 cut(s) 305
MseI TTAA 2 cut(s) 138, 686
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 1 cut(s) 306
MspR9I CCNGG 1 cut(s) 343
MunI CAATTG 2 cut(s) 189, 644
Mva1269I GAATGC 2 cut(s) 260, 968
MvaI CCWGG 1 cut(s) 343
MwoI GCNNNNNNNGC 1 cut(s) 965
NaeI GCCGGC 1 cut(s) 307
NcoI CCATGG 1 cut(s) 822
NdeII GATC 1 cut(s) 769
NgoMIV GCCGGC 1 cut(s) 305
NheI GCTAGC 1 cut(s) 923
NlaIII CATG 5 cut(s) 431, 826, 838, 895, 950
NlaIV GGNNCC 2 cut(s) 211, 358
NmuCI GTSAC 2 cut(s) 453, 840
NsiI ATGCAT 1 cut(s) 900
PciSI GCTCTTC 1 cut(s) 38
PctI GAATGC 2 cut(s) 260, 968
PdiI GCCGGC 1 cut(s) 307
PkrI GCNGC 3 cut(s) 247, 300, 922
PleI GAGTC 1 cut(s) 788
PpsI GAGTC 1 cut(s) 788
Ppu21I YACGTR 2 cut(s) 437, 694
PsiI TTATAA 1 cut(s) 548
Psp1406I AACGTT 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 341
PspFI CCCAGC 1 cut(s) 563
PspGI CCWGG 1 cut(s) 341
PspN4I GGNNCC 2 cut(s) 211, 358
PspPI GGNCC 1 cut(s) 185
PstI CTGCAG 1 cut(s) 247
PvuII CAGCTG 1 cut(s) 248
SapI GCTCTTC 1 cut(s) 38
SaqAI TTAA 2 cut(s) 138, 686
SatI GCNGC 3 cut(s) 246, 299, 921
Sau3AI GATC 1 cut(s) 769
Sau96I GGNCC 1 cut(s) 185
SchI GAGTC 1 cut(s) 788
ScrFI CCNGG 1 cut(s) 343
SfaNI GCATC 2 cut(s) 885, 936
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 1 cut(s) 185
SmlI CTYRAG 3 cut(s) 53, 373, 710
SmoI CTYRAG 3 cut(s) 53, 373, 710
SnaBI TACGTA 1 cut(s) 694
Sse9I AATT 4 cut(s) 189, 203, 624, 644
SsiI CCGC 1 cut(s) 214
SspI AATATT 1 cut(s) 729
SspMI CTAG 1 cut(s) 924
StyD4I CCNGG 1 cut(s) 341
StyI CCWWGG 2 cut(s) 316, 822
TaaI ACNGT 2 cut(s) 290, 337
TaiI ACGT 3 cut(s) 439, 666, 696
TaqI TCGA 1 cut(s) 475
TasI AATT 4 cut(s) 189, 203, 624, 644
Tru1I TTAA 2 cut(s) 138, 686
Tru9I TTAA 2 cut(s) 138, 686
TscAI CASTG 2 cut(s) 368, 849
TseFI GTSAC 2 cut(s) 453, 840
TseI GCWGC 3 cut(s) 245, 298, 920
Tsp45I GTSAC 2 cut(s) 453, 840
TspDTI ATGAA 6 cut(s) 25, 416, 459, 527, 664, 891
TspRI CASTG 2 cut(s) 368, 849
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 1 cut(s) 924
Zsp2I ATGCAT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.