RchiOBHm_Chr6g0312311

Belongs to the peptidase C19 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0022172
Physical Location & Seq
Forward (+)
68975854 .. 68977952
2099 bp
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UTR
Exon/CDS
Intron
PRQ28094

Sequence Viewer

Length: 951 bp
ATGAAGCAAGTTGCTGAGGATGCCATTGCAGGGAGGTGTTCAGATGGAACATACTATGTTTCTAGATCATGGTTGCAGCAGTGGCTTAAAAGAAAAATTCTTGATGCTCCATCTGAAGCTGATGCAGGACCAACAGTTTCAATCAGGTGTCCTCATGGACAGCTTTTGCCTGAGCAAGCCCCTGGTGCTAAGAGATTGCTTATTCCAGAGATCCTCTGGCTCTTCATTTATCAAGATGCTTTAACAGTAAAACCTGCTGAGGACCTGGGTTGTTCAACTTTTCTCTCAGACTCTCTACAGTGTTCTGAGTGCAGTGATGAACTATCTGAAGTTGCATGCATGGAGGATTCTTTAAGATTGGTCAGGGAAAAACAGCGCCAGACTCATGATAAATTGGCTGTCGGGAAAAGTATTCCTCTTTCTTTGCATTGCAAGTATTACTTGATACCCTATTCTTGGCTTTCAAAATGGAGAACCTACATCAACGCAGCCGGAAGGAATATTTCTTCAGTAGAGAAACCGGAAACATTATATGGCATTATGGATTTGATCAAGTGTGAAAAGCACTCACGACTTCTAGAAAGGCCTGTTGATCTGGTCTGTAAACGTGGTTTGATTTCACCAAAGTCTTCCCCTGTGGATGGGTTAATATTCATTACTGAGAGTGACTGGAAATCCTTCTGTGAGGAATGGGGTGGTATTGAGGAAAAAGGTGTATCTGCTGAAATCAAGTTAAGTAATACTGAAGGAAATAACTTGGCAGAGTCCTCTGATGATGTGCAAATCTGTGAGGAGGACTTGAGTACTCCAAATCTCGTGAATAGTGAAGTTGATTCTAGATCGCTTGTGATTAGGACTTTCCCTGAGATATGTGAGGACTGCATTGGAGAGAAAGAAAGCAGGGAGCTAATGCGAAAGCTTGAGTACTGCAATGAGGATATATATGTATAA

Protein Analysis

316

Amino Acids

35.68

Weight (kDa)

4.88

Isoelectric Point (pI)

56.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUSP PF06337 145 - 234 8.6e-06 DUSP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022172)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30650
rosa_chinensis RchiOBHm_Chr6g0312311
rosa_samantha Rh6AG512200 Rh6AG514500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 262
AclWI GGATC 1 cut(s) 205
AcsI RAATTY 1 cut(s) 96
AcuI CTGAAG 4 cut(s) 135, 348, 492, 765
AfaI GTAC 2 cut(s) 805, 926
AfiI CCNNNNNNNGG 3 cut(s) 30, 456, 641
AgsI TTSAA 3 cut(s) 141, 276, 465
AjnI CCWGG 2 cut(s) 181, 264
AluBI AGCT 4 cut(s) 119, 163, 907, 919
AluI AGCT 4 cut(s) 119, 163, 907, 919
AlwI GGATC 1 cut(s) 205
AoxI GGCC 1 cut(s) 584
ApeKI GCWGC 2 cut(s) 76, 488
ApoI RAATTY 1 cut(s) 96
Asp700I GAANNNNTTC 1 cut(s) 677
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 2 cut(s) 128, 262
AsuHPI GGTGA 1 cut(s) 612
AvaII GGWCC 2 cut(s) 128, 262
BauI CACGAG 1 cut(s) 815
BbsI GAAGAC 1 cut(s) 621
BbvCI CCTCAGC 2 cut(s) 15, 258
BbvI GCAGC 2 cut(s) 88, 500
BccI CCATC 3 cut(s) 38, 118, 635
BciT130I CCWGG 2 cut(s) 183, 266
BclI TGATCA 1 cut(s) 549
BfaI CTAG 3 cut(s) 63, 578, 837
BfmI CTRYAG 1 cut(s) 296
BfoI RGCGCY 1 cut(s) 379
BfuAI ACCTGC 1 cut(s) 262
BisI GCNGC 2 cut(s) 77, 489
BlsI GCNGC 2 cut(s) 78, 490
BmcAI AGTACT 2 cut(s) 805, 926
Bme1390I CCNGG 2 cut(s) 183, 266
Bme18I GGWCC 2 cut(s) 128, 262
BmgT120I GGNCC 2 cut(s) 128, 262
BmrFI CCNGG 2 cut(s) 183, 266
BmsI GCATC 4 cut(s) 10, 94, 112, 226
BpiI GAAGAC 1 cut(s) 621
Bpu10I CCTNAGC 3 cut(s) 15, 171, 258
BpuEI CTTGAG 2 cut(s) 820, 941
BsaJI CCNNGG 2 cut(s) 181, 265
BsaWI WCCGGW 1 cut(s) 520
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 456, 641
Bse1I ACTGG 1 cut(s) 674
Bse3DI GCAATG 3 cut(s) 24, 427, 937
BseBI CCWGG 2 cut(s) 183, 266
BseDI CCNNGG 2 cut(s) 181, 265
BseGI GGATG 2 cut(s) 25, 646
BseLI CCNNNNNNNGG 3 cut(s) 30, 456, 641
BseMI GCAATG 3 cut(s) 24, 427, 937
BseMII CTCAG 7 cut(s) 6, 162, 249, 297, 300, 651, 855
BseNI ACTGG 1 cut(s) 674
BseRI GAGGAG 1 cut(s) 806
BseXI GCAGC 2 cut(s) 88, 500
BsgI GTGCAG 1 cut(s) 331
BshFI GGCC 1 cut(s) 586
BsiSI CCGG 2 cut(s) 492, 521
BslI CCNNNNNNNGG 3 cut(s) 30, 456, 641
BsnI GGCC 1 cut(s) 586
Bsp143I GATC 5 cut(s) 65, 210, 549, 592, 839
BspANI GGCC 1 cut(s) 586
BspCNI CTCAG 7 cut(s) 7, 163, 250, 298, 299, 652, 856
BspHI TCATGA 1 cut(s) 385
BspMI ACCTGC 1 cut(s) 262
BspPI GGATC 1 cut(s) 205
BspQI GCTCTTC 1 cut(s) 227
BsrDI GCAATG 3 cut(s) 24, 427, 937
BsrI ACTGG 1 cut(s) 674
BssECI CCNNGG 2 cut(s) 181, 265
BssMI GATC 5 cut(s) 65, 210, 549, 592, 839
BssSI CACGAG 1 cut(s) 815
Bst2BI CACGAG 1 cut(s) 815
Bst2UI CCWGG 2 cut(s) 183, 266
Bst4CI ACNGT 3 cut(s) 136, 247, 300
Bst6I CTCTTC 1 cut(s) 227
BstC8I GCNNGC 2 cut(s) 177, 337
BstDEI CTNAG 8 cut(s) 15, 171, 189, 258, 286, 306, 660, 864
BstF5I GGATG 2 cut(s) 25, 646
BstH2I RGCGCY 1 cut(s) 379
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 5 cut(s) 68, 213, 552, 595, 842
BstMBI GATC 5 cut(s) 65, 210, 549, 592, 839
BstMWI GCNNNNNNNGC 3 cut(s) 20, 82, 185
BstNI CCWGG 2 cut(s) 183, 266
BstNSI RCATGY 1 cut(s) 339
BstSCI CCNGG 2 cut(s) 181, 264
BstSFI CTRYAG 1 cut(s) 296
BstV1I GCAGC 2 cut(s) 88, 500
BstV2I GAAGAC 1 cut(s) 621
BstX2I RGATCY 1 cut(s) 210
BstYI RGATCY 1 cut(s) 210
BsuRI GGCC 1 cut(s) 586
BtsCI GGATG 2 cut(s) 25, 646
BtsI GCAGTG 2 cut(s) 86, 319
BtsIMutI CAGTG 3 cut(s) 86, 305, 319
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 2 cut(s) 177, 337
CciI TCATGA 1 cut(s) 385
CfoI GCGC 1 cut(s) 378
Cfr13I GGNCC 2 cut(s) 128, 262
Csp6I GTAC 2 cut(s) 804, 925
CviAII CATG 5 cut(s) 69, 155, 336, 340, 386
CviQI GTAC 2 cut(s) 804, 925
DdeI CTNAG 8 cut(s) 15, 171, 189, 258, 286, 306, 660, 864
DpnI GATC 5 cut(s) 67, 212, 551, 594, 841
DpnII GATC 5 cut(s) 65, 210, 549, 592, 839
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco147I AGGCCT 1 cut(s) 586
Eco47I GGWCC 2 cut(s) 128, 262
Eco57I CTGAAG 4 cut(s) 135, 348, 492, 765
EcoO109I RGGNCCY 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 181, 264
EcoT22I ATGCAT 1 cut(s) 341
FaeI CATG 5 cut(s) 72, 158, 339, 343, 389
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FatI CATG 5 cut(s) 68, 154, 335, 339, 385
FbaI TGATCA 1 cut(s) 549
Fnu4HI GCNGC 2 cut(s) 77, 489
FokI GGATG 2 cut(s) 32, 653
Fsp4HI GCNGC 2 cut(s) 77, 489
FspBI CTAG 3 cut(s) 63, 578, 837
GlaI GCGC 1 cut(s) 377
GluI GCNGC 2 cut(s) 77, 489
HaeII RGCGCY 1 cut(s) 379
HaeIII GGCC 1 cut(s) 586
HapII CCGG 2 cut(s) 492, 521
HhaI GCGC 1 cut(s) 378
Hin1II CATG 5 cut(s) 72, 158, 339, 343, 389
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 917
HinfI GANTC 5 cut(s) 290, 347, 382, 764, 833
HpaII CCGG 2 cut(s) 492, 521
HphI GGTGA 1 cut(s) 612
Hpy166II GTNNAC 1 cut(s) 605
Hpy188I TCNGA 6 cut(s) 43, 115, 289, 307, 328, 772
Hpy8I GTNNAC 1 cut(s) 605
HpyAV CCTTC 3 cut(s) 489, 688, 740
HpyCH4III ACNGT 3 cut(s) 136, 247, 300
HpyCH4IV ACGT 1 cut(s) 607
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 82, 185
HpyF3I CTNAG 8 cut(s) 15, 171, 189, 258, 286, 306, 660, 864
HpySE526I ACGT 1 cut(s) 607
Hsp92II CATG 5 cut(s) 72, 158, 339, 343, 389
HspAI GCGC 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 5 cut(s) 65, 210, 549, 592, 839
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 2 cut(s) 112, 904
Lsp1109I GCAGC 2 cut(s) 88, 500
LweI GCATC 4 cut(s) 10, 94, 112, 226
MaeI CTAG 3 cut(s) 63, 578, 837
MaeII ACGT 1 cut(s) 607
MaeIII GTNAC 1 cut(s) 665
MalI GATC 5 cut(s) 67, 212, 551, 594, 841
MboI GATC 5 cut(s) 65, 210, 549, 592, 839
MboII GAAGA 3 cut(s) 214, 498, 621
MflI RGATCY 1 cut(s) 210
MluCI AATT 2 cut(s) 96, 392
MlyI GAGTC 3 cut(s) 284, 376, 773
Mph1103I ATGCAT 1 cut(s) 341
MroXI GAANNNNTTC 1 cut(s) 677
MseI TTAA 5 cut(s) 87, 242, 353, 647, 734
MspI CCGG 2 cut(s) 492, 521
MspR9I CCNGG 2 cut(s) 183, 266
MvaI CCWGG 2 cut(s) 183, 266
MwoI GCNNNNNNNGC 3 cut(s) 20, 82, 185
NdeII GATC 5 cut(s) 65, 210, 549, 592, 839
NlaIII CATG 5 cut(s) 72, 158, 339, 343, 389
NmuCI GTSAC 1 cut(s) 665
NsiI ATGCAT 1 cut(s) 341
NspI RCATGY 1 cut(s) 339
PaeI GCATGC 1 cut(s) 339
PagI TCATGA 1 cut(s) 385
PceI AGGCCT 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 677
PfeI GAWTC 2 cut(s) 347, 833
PkrI GCNGC 2 cut(s) 78, 490
PleI GAGTC 3 cut(s) 284, 376, 772
PpsI GAGTC 3 cut(s) 284, 376, 772
PpuMI RGGWCCY 1 cut(s) 262
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 2 cut(s) 181, 264
PspGI CCWGG 2 cut(s) 181, 264
PspPI GGNCC 2 cut(s) 128, 262
PspPPI RGGWCCY 1 cut(s) 262
PsuI RGATCY 1 cut(s) 210
RsaI GTAC 2 cut(s) 805, 926
RsaNI GTAC 2 cut(s) 804, 925
SapI GCTCTTC 1 cut(s) 227
SaqAI TTAA 5 cut(s) 87, 242, 353, 647, 734
SatI GCNGC 2 cut(s) 77, 489
Sau3AI GATC 5 cut(s) 65, 210, 549, 592, 839
Sau96I GGNCC 2 cut(s) 128, 262
ScaI AGTACT 2 cut(s) 805, 926
SchI GAGTC 3 cut(s) 284, 376, 773
ScrFI CCNGG 2 cut(s) 183, 266
SfaNI GCATC 4 cut(s) 10, 94, 112, 226
SfcI CTRYAG 1 cut(s) 296
SinI GGWCC 2 cut(s) 128, 262
SmlI CTYRAG 2 cut(s) 799, 920
SmoI CTYRAG 2 cut(s) 799, 920
SphI GCATGC 1 cut(s) 339
Sse9I AATT 2 cut(s) 96, 392
SseBI AGGCCT 1 cut(s) 586
SspI AATATT 2 cut(s) 502, 651
SspMI CTAG 3 cut(s) 63, 578, 837
StuI AGGCCT 1 cut(s) 586
StyD4I CCNGG 2 cut(s) 181, 264
TaaI ACNGT 3 cut(s) 136, 247, 300
TaiI ACGT 1 cut(s) 610
TasI AATT 2 cut(s) 96, 392
TatI WGTACW 2 cut(s) 803, 924
TfiI GAWTC 2 cut(s) 347, 833
Tru1I TTAA 5 cut(s) 87, 242, 353, 647, 734
Tru9I TTAA 5 cut(s) 87, 242, 353, 647, 734
TscAI CASTG 3 cut(s) 86, 305, 319
TseFI GTSAC 1 cut(s) 665
TseI GCWGC 2 cut(s) 76, 488
Tsp45I GTSAC 1 cut(s) 665
TspDTI ATGAA 4 cut(s) 17, 214, 333, 643
TspRI CASTG 3 cut(s) 86, 305, 319
VpaK11BI GGWCC 2 cut(s) 128, 262
XapI RAATTY 1 cut(s) 96
XbaI TCTAGA 3 cut(s) 62, 577, 836
XceI RCATGY 1 cut(s) 339
XcmI CCANNNNNNNNNTGG 1 cut(s) 213
XmnI GAANNNNTTC 1 cut(s) 677
XspI CTAG 3 cut(s) 63, 578, 837
ZrmI AGTACT 2 cut(s) 805, 926
Zsp2I ATGCAT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.