Rh6AG514500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0022172
Physical Location & Seq
Reverse (-)
68476382 .. 68481142
4761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG514500.1

Sequence Viewer

Length: 783 bp
ATGTCCATCCATACCACCAATGGCCGAAACGGCCAAGTAAGTAGTACCTCGGATCATCCTGAAAATGAGAAGCAGGCATGTAGTAAACACAGCGACCACTATCCCTCCAAAGATGATGAACGGCGTGGGAATGCAGTCACATCTCTGGGTCTCCTCAGTGCTAGTGCAGACACACGTACTTCTCGAATTGCACCGAATGCGACCTCCGCCGGCTCTCCCGCCTCAGCCTTTTCACCCACCTCCGCCGTTTCCCCAGCTCCGGTGGACAAGATCACAGTTGAGAGTCATGCGTCGACAATCCGGAGCCGACGCAGGACCACAAATGACGGCAGCCGTGCTGTTAGCGCTATCGTGGCCTCACTTCTCAAAGCGCAAGAGAAGAGGTACGTTAAGCATCAGGATGCATTTCCCCTTCAATCCAGCGAAGTCCCCCCCCGCCACAGCAGTCCCCTTCATCTCGTCAACAGTGTACTAATGGCCATGTCCGACATGGTCAAGATGCTGGCCCTTATGATGATGACTGTAATCCAGATGCTTGCAGCTCCACCTGAGGATATGTTTTCATCTCCTCATCATGGGCTGGCGTGCACTTGCACCGGTGTTGGGGAATCTGAAGATTGTCAGTGCCTCCCGATTCTATTTATCATCTTTCCGGGAGTAATGTCAACCGCGTTCACATATGAACACATGCAACATTACTCCAAAGTTTCAAGATTTGTTTGGTATCACTCATATTTGTCCCTTCACTTTCACACTTGGACTCTTTTAAGTGGTTTAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

28.4

Weight (kDa)

8.29

Isoelectric Point (pI)

61.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022172)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30650
rosa_chinensis RchiOBHm_Chr6g0312311
rosa_samantha Rh6AG512200 Rh6AG514500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 293
AccII CGCG 1 cut(s) 671
AccIII TCCGGA 1 cut(s) 300
AciI CCGC 5 cut(s) 207, 219, 243, 436, 669
AclWI GGATC 1 cut(s) 60
AcoI YGGCCR 3 cut(s) 22, 31, 477
AcuI CTGAAG 1 cut(s) 633
AfaI GTAC 4 cut(s) 46, 178, 386, 471
AfeI AGCGCT 1 cut(s) 346
AfiI CCNNNNNNNGG 3 cut(s) 259, 575, 603
AflIII ACRYGT 1 cut(s) 173
AgeI ACCGGT 1 cut(s) 596
AgsI TTSAA 2 cut(s) 416, 711
AluBI AGCT 2 cut(s) 257, 542
AluI AGCT 2 cut(s) 257, 542
Alw21I GWGCWC 1 cut(s) 590
Alw26I GTCTC 1 cut(s) 155
Alw44I GTGCAC 1 cut(s) 586
AlwI GGATC 1 cut(s) 60
Aor13HI TCCGGA 1 cut(s) 300
Aor51HI AGCGCT 1 cut(s) 346
AoxI GGCC 5 cut(s) 22, 31, 354, 477, 504
ApaLI GTGCAC 1 cut(s) 586
ApeKI GCWGC 2 cut(s) 330, 539
AsiGI ACCGGT 1 cut(s) 596
AspLEI GCGC 2 cut(s) 347, 373
AspS9I GGNCC 2 cut(s) 315, 505
AsuC2I CCSGG 1 cut(s) 654
AsuHPI GGTGA 1 cut(s) 225
AvaII GGWCC 1 cut(s) 315
AxyI CCTNAGG 1 cut(s) 549
BaeGI GKGCMC 1 cut(s) 590
BalI TGGCCA 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 590
BbvCI CCTCAGC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 342, 551
BccI CCATC 1 cut(s) 14
BceAI ACGGC 5 cut(s) 46, 137, 230, 318, 343
BcnI CCSGG 1 cut(s) 654
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 1 cut(s) 162
BfoI RGCGCY 1 cut(s) 348
BglI GCCNNNNNGGC 1 cut(s) 30
BisI GCNGC 2 cut(s) 331, 540
BlsI GCNGC 2 cut(s) 332, 541
Bme1390I CCNGG 1 cut(s) 654
Bme18I GGWCC 1 cut(s) 315
BmgT120I GGNCC 2 cut(s) 315, 505
BmiI GGNNCC 1 cut(s) 305
BmrFI CCNGG 1 cut(s) 654
BmsI GCATC 4 cut(s) 391, 403, 489, 522
Bpu10I CCTNAGC 1 cut(s) 223
BpuMI CCSGG 1 cut(s) 654
BsaAI YACGTR 1 cut(s) 176
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 48
BsaWI WCCGGW 3 cut(s) 259, 300, 596
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 3 cut(s) 259, 575, 603
Bse118I RCCGGY 2 cut(s) 209, 596
Bse21I CCTNAGG 1 cut(s) 549
BseAI TCCGGA 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 3 cut(s) 6, 55, 406
BseLI CCNNNNNNNGG 3 cut(s) 259, 575, 603
BseMII CTCAG 3 cut(s) 169, 237, 540
BseRI GAGGAG 2 cut(s) 143, 558
BseSI GKGCMC 1 cut(s) 590
BseXI GCAGC 2 cut(s) 342, 551
BseYI CCCAGC 1 cut(s) 253
BsgI GTGCAG 1 cut(s) 186
Bsh1236I CGCG 1 cut(s) 671
BshFI GGCC 5 cut(s) 24, 33, 356, 479, 506
BshTI ACCGGT 1 cut(s) 596
BsiHKAI GWGCWC 1 cut(s) 590
BsiSI CCGG 5 cut(s) 210, 260, 301, 597, 653
BslFI GGGAC 3 cut(s) 413, 432, 724
BslI CCNNNNNNNGG 3 cut(s) 259, 575, 603
BsmAI GTCTC 1 cut(s) 155
BsmFI GGGAC 3 cut(s) 413, 432, 724
BsmI GAATGC 2 cut(s) 136, 202
BsnI GGCC 5 cut(s) 24, 33, 356, 479, 506
Bso31I GGTCTC 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 590
Bsp13I TCCGGA 1 cut(s) 300
Bsp143I GATC 2 cut(s) 52, 270
BspACI CCGC 5 cut(s) 207, 219, 243, 436, 669
BspANI GGCC 5 cut(s) 24, 33, 356, 479, 506
BspCNI CTCAG 3 cut(s) 168, 236, 541
BspEI TCCGGA 1 cut(s) 300
BspFNI CGCG 1 cut(s) 671
BspLI GGNNCC 1 cut(s) 305
BspPI GGATC 1 cut(s) 60
BspTNI GGTCTC 1 cut(s) 155
BsrFI RCCGGY 2 cut(s) 209, 596
BssAI RCCGGY 2 cut(s) 209, 596
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 2 cut(s) 52, 270
Bst4CI ACNGT 3 cut(s) 277, 467, 523
Bst6I CTCTTC 1 cut(s) 374
BstAPI GCANNNNNTGC 1 cut(s) 197
BstBAI YACGTR 1 cut(s) 176
BstC8I GCNNGC 6 cut(s) 75, 211, 504, 537, 582, 586
BstDEI CTNAG 3 cut(s) 155, 223, 549
BstF5I GGATG 3 cut(s) 6, 55, 406
BstFNI CGCG 1 cut(s) 671
BstH2I RGCGCY 1 cut(s) 348
BstHHI GCGC 2 cut(s) 347, 373
BstKTI GATC 2 cut(s) 55, 273
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 2 cut(s) 52, 270
BstMWI GCNNNNNNNGC 5 cut(s) 30, 197, 206, 344, 353
BstNSI RCATGY 2 cut(s) 81, 691
BstSCI CCNGG 1 cut(s) 652
BstSLI GKGCMC 1 cut(s) 590
BstUI CGCG 1 cut(s) 671
BstV1I GCAGC 2 cut(s) 342, 551
Bsu36I CCTNAGG 1 cut(s) 549
BsuRI GGCC 5 cut(s) 24, 33, 356, 479, 506
BtsCI GGATG 3 cut(s) 6, 55, 406
BtsIMutI CAGTG 3 cut(s) 163, 472, 629
Cac8I GCNNGC 6 cut(s) 75, 211, 504, 537, 582, 586
CfoI GCGC 2 cut(s) 347, 373
Cfr10I RCCGGY 2 cut(s) 209, 596
Cfr13I GGNCC 2 cut(s) 315, 505
CseI GACGC 2 cut(s) 279, 318
Csp6I GTAC 4 cut(s) 45, 177, 385, 470
CspAI ACCGGT 1 cut(s) 596
CviAII CATG 6 cut(s) 78, 287, 481, 490, 575, 688
CviQI GTAC 4 cut(s) 45, 177, 385, 470
DdeI CTNAG 3 cut(s) 155, 223, 549
DpnI GATC 2 cut(s) 54, 272
DpnII GATC 2 cut(s) 52, 270
DraI TTTAAA 1 cut(s) 777
EaeI YGGCCR 3 cut(s) 22, 31, 477
Eam1104I CTCTTC 1 cut(s) 374
EarI CTCTTC 1 cut(s) 374
EciI GGCGGA 2 cut(s) 196, 232
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 1 cut(s) 315
Eco47III AGCGCT 1 cut(s) 346
Eco57I CTGAAG 1 cut(s) 633
Eco81I CCTNAGG 1 cut(s) 549
EcoT22I ATGCAT 1 cut(s) 406
FaeI CATG 6 cut(s) 81, 290, 484, 493, 578, 691
FaqI GGGAC 3 cut(s) 413, 432, 724
FatI CATG 6 cut(s) 77, 286, 480, 489, 574, 687
FauI CCCGC 2 cut(s) 226, 443
FauNDI CATATG 1 cut(s) 679
FblI GTMKAC 1 cut(s) 293
Fnu4HI GCNGC 2 cut(s) 331, 540
FokI GGATG 2 cut(s) 42, 413
Fsp4HI GCNGC 2 cut(s) 331, 540
FspBI CTAG 1 cut(s) 162
GlaI GCGC 2 cut(s) 346, 372
GluI GCNGC 2 cut(s) 331, 540
GsaI CCCAGC 1 cut(s) 257
HaeII RGCGCY 1 cut(s) 348
HaeIII GGCC 5 cut(s) 24, 33, 356, 479, 506
HapII CCGG 5 cut(s) 210, 260, 301, 597, 653
HgaI GACGC 2 cut(s) 279, 318
HhaI GCGC 2 cut(s) 347, 373
Hin1II CATG 6 cut(s) 81, 290, 484, 493, 578, 691
Hin6I GCGC 2 cut(s) 345, 371
HinP1I GCGC 2 cut(s) 345, 371
HincII GTYRAC 3 cut(s) 294, 463, 666
HindII GTYRAC 3 cut(s) 294, 463, 666
HinfI GANTC 4 cut(s) 283, 608, 634, 760
HpaII CCGG 5 cut(s) 210, 260, 301, 597, 653
HphI GGTGA 1 cut(s) 225
Hpy166II GTNNAC 8 cut(s) 86, 265, 294, 463, 470, 588, 666, 675
Hpy188I TCNGA 3 cut(s) 52, 487, 613
Hpy188III TCNNGA 8 cut(s) 59, 183, 301, 398, 496, 529, 631, 711
Hpy8I GTNNAC 8 cut(s) 86, 265, 294, 463, 470, 588, 666, 675
Hpy99I CGWCG 2 cut(s) 295, 312
HpyAV CCTTC 3 cut(s) 422, 461, 752
HpyCH4III ACNGT 3 cut(s) 277, 467, 523
HpyCH4IV ACGT 2 cut(s) 175, 387
HpyCH4V TGCA 8 cut(s) 134, 167, 191, 404, 539, 588, 594, 691
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 197, 206, 344, 353
HpyF3I CTNAG 3 cut(s) 155, 223, 549
HpySE526I ACGT 2 cut(s) 175, 387
Hsp92II CATG 6 cut(s) 81, 290, 484, 493, 578, 691
HspAI GCGC 2 cut(s) 345, 371
Kpn2I TCCGGA 1 cut(s) 300
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Kzo9I GATC 2 cut(s) 52, 270
LmnI GCTCC 3 cut(s) 262, 303, 547
Lsp1109I GCAGC 2 cut(s) 342, 551
LweI GCATC 4 cut(s) 391, 403, 489, 522
MaeI CTAG 1 cut(s) 162
MaeII ACGT 2 cut(s) 175, 387
MaeIII GTNAC 1 cut(s) 136
MalI GATC 2 cut(s) 54, 272
MboI GATC 2 cut(s) 52, 270
MboII GAAGA 2 cut(s) 391, 626
MhlI GDGCHC 1 cut(s) 590
MlsI TGGCCA 1 cut(s) 479
MluCI AATT 1 cut(s) 186
MluNI TGGCCA 1 cut(s) 479
MlyI GAGTC 2 cut(s) 292, 754
MmeI TCCRAC 1 cut(s) 510
Mox20I TGGCCA 1 cut(s) 479
Mph1103I ATGCAT 1 cut(s) 406
MroI TCCGGA 1 cut(s) 300
MroNI GCCGGC 1 cut(s) 209
MscI TGGCCA 1 cut(s) 479
MseI TTAA 3 cut(s) 390, 767, 776
MslI CAYNNNNRTG 1 cut(s) 399
Msp20I TGGCCA 1 cut(s) 479
MspI CCGG 5 cut(s) 210, 260, 301, 597, 653
MspR9I CCNGG 1 cut(s) 654
Mva1269I GAATGC 2 cut(s) 136, 202
MvnI CGCG 1 cut(s) 671
MwoI GCNNNNNNNGC 5 cut(s) 30, 197, 206, 344, 353
NaeI GCCGGC 1 cut(s) 211
NciI CCSGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 679
NdeII GATC 2 cut(s) 52, 270
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 6 cut(s) 81, 290, 484, 493, 578, 691
NlaIV GGNNCC 1 cut(s) 305
NmuCI GTSAC 1 cut(s) 136
NsiI ATGCAT 1 cut(s) 406
NspI RCATGY 2 cut(s) 81, 691
PcsI WCGNNNNNNNCGW 1 cut(s) 181
PctI GAATGC 2 cut(s) 136, 202
PdiI GCCGGC 1 cut(s) 211
PfeI GAWTC 2 cut(s) 608, 634
PflFI GACNNNGTC 1 cut(s) 491
PfoI TCCNGGA 1 cut(s) 652
PinAI ACCGGT 1 cut(s) 596
PkrI GCNGC 2 cut(s) 332, 541
PleI GAGTC 2 cut(s) 291, 754
PpsI GAGTC 2 cut(s) 291, 754
Ppu21I YACGTR 1 cut(s) 176
PspFI CCCAGC 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 305
PspPI GGNCC 2 cut(s) 315, 505
PsyI GACNNNGTC 1 cut(s) 491
RsaI GTAC 4 cut(s) 46, 178, 386, 471
RsaNI GTAC 4 cut(s) 45, 177, 385, 470
RseI CAYNNNNRTG 1 cut(s) 399
SalI GTCGAC 1 cut(s) 292
SaqAI TTAA 3 cut(s) 390, 767, 776
SatI GCNGC 2 cut(s) 331, 540
Sau3AI GATC 2 cut(s) 52, 270
Sau96I GGNCC 2 cut(s) 315, 505
SchI GAGTC 2 cut(s) 292, 754
ScrFI CCNGG 1 cut(s) 654
SduI GDGCHC 1 cut(s) 590
SetI ASST 9 cut(s) 50, 178, 206, 242, 259, 386, 390, 544, 550
SfaNI GCATC 4 cut(s) 391, 403, 489, 522
SfiI GGCCNNNNNGGCC 1 cut(s) 30
SgrAI CRCCGGYG 1 cut(s) 596
SinI GGWCC 1 cut(s) 315
SmiMI CAYNNNNRTG 1 cut(s) 399
Sse9I AATT 1 cut(s) 186
SsiI CCGC 5 cut(s) 207, 219, 243, 436, 669
SspMI CTAG 1 cut(s) 162
StyD4I CCNGG 1 cut(s) 652
TaaI ACNGT 3 cut(s) 277, 467, 523
TaiI ACGT 2 cut(s) 178, 390
TaqI TCGA 2 cut(s) 184, 293
TasI AATT 1 cut(s) 186
TatI WGTACW 1 cut(s) 469
TfiI GAWTC 2 cut(s) 608, 634
Tru1I TTAA 3 cut(s) 390, 767, 776
Tru9I TTAA 3 cut(s) 390, 767, 776
TscAI CASTG 3 cut(s) 163, 472, 629
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 2 cut(s) 330, 539
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 4 cut(s) 132, 443, 552, 696
TspRI CASTG 3 cut(s) 163, 472, 629
Tth111I GACNNNGTC 1 cut(s) 491
VneI GTGCAC 1 cut(s) 586
VpaK11BI GGWCC 1 cut(s) 315
XceI RCATGY 2 cut(s) 81, 691
XcmI CCANNNNNNNNNTGG 2 cut(s) 17, 487
XmiI GTMKAC 1 cut(s) 293
XspI CTAG 1 cut(s) 162
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.