RCHIOBHM_MTG0498351

ATP synthase protein MI25-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Mt
OG0019174
Physical Location & Seq
Forward (+)
60920 .. 61683
764 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15607

Sequence Viewer

Length: 597 bp
ATGAGATTTACTTCCACGAATACGCATGCTAGAAAGATGCTATTTGCTGCAATTCCATCTATTTGTGCATTAAGTTCGAAGAAGATCTCAATCTATAATGAAGAAATGATAGTAGCTCTTTGTTTTATAGGCTTTATCATATTGAGTCGTAAGAGTTTAGGGGATACTTTCAAAATGACTCTCGACGGTAGAATCCAGGCTATTCAGGAAGAATCGCAGCAATTCCCCAATCCTAACGAAGTAGTTCCTCTGGAATCCAATGAACAACAACGATTCCTTAGGATCAGTTTACGAATTTGTGGCACCGTAGTAGAATCATTACCAATTGCACGCTGTGCGCCTAAGTGCGAAAAGACAGTGCAAGCTTTGTTATGCCGAAACCTAAATGTTAAGTCAGCAACACTTCCAAATGCCATTTCTTCCCGTCGCATCCGTCTTCAGGACGATCTAGTCACAGGGTTTCATTTCTCAGTGAGTGAAAGATTTGTCCCTGGGTGTACGTTGAAAGCTTCTATAGTAGAACTCATTCGAGAGGGCTTGGTGGTCTTAAGAATGGTTCTGGTGGGGGGTTCCCTTAAGAATAAAGAAGACGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mt_ATP-synt_B

Protein Analysis

198

Amino Acids

22.11

Weight (kDa)

9.21

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mt_ATP-synt_B PF05405 20 - 183 9.8e-49 Mitochondrial ATP synthase B chain precursor (ATP-synt_B)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019174)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00640
rosa_chinensis RchiOBHm_MTg0498351
rosa_laevigata RLG00000000385 RLG00000000401
rosa_multiflora Rmu_sc0022700.1_g000004
rosa_roxburghii Rroxscaffold_2G00119440
rosa_samantha Rh1BG019400 Rh4BG135100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 449
AccB1I GGYRCC 1 cut(s) 302
AclWI GGATC 1 cut(s) 290
AcsI RAATTY 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 422
AdeI CACNNNGTG 1 cut(s) 335
AfaI GTAC 1 cut(s) 499
AfiI CCNNNNNNNGG 1 cut(s) 439
AflII CTTAAG 2 cut(s) 547, 575
AgsI TTSAA 2 cut(s) 172, 505
AjnI CCWGG 2 cut(s) 195, 490
AjuI GAANNNNNNNTTGG 2 cut(s) 400, 432
AluBI AGCT 3 cut(s) 116, 365, 509
AluI AGCT 3 cut(s) 116, 365, 509
AlwI GGATC 1 cut(s) 290
ApeKI GCWGC 2 cut(s) 47, 217
ApoI RAATTY 1 cut(s) 294
Asp700I GAANNNNTTC 2 cut(s) 243, 525
AspLEI GCGC 1 cut(s) 340
AsuII TTCGAA 1 cut(s) 77
AxyI CCTNAGG 1 cut(s) 278
BanI GGYRCC 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 428
BbvI GCAGC 2 cut(s) 34, 229
BccI CCATC 1 cut(s) 64
BcgI CGANNNNNNTGC 2 cut(s) 57, 91
BciT130I CCWGG 2 cut(s) 197, 492
BciVI GTATCC 1 cut(s) 157
BfaI CTAG 2 cut(s) 30, 449
BfmI CTRYAG 1 cut(s) 513
BfrI CTTAAG 2 cut(s) 547, 575
BfuI GTATCC 1 cut(s) 157
BglII AGATCT 1 cut(s) 84
BisI GCNGC 2 cut(s) 48, 218
BlsI GCNGC 2 cut(s) 49, 219
Bme1390I CCNGG 2 cut(s) 197, 492
BmiI GGNNCC 2 cut(s) 304, 571
BmrFI CCNGG 2 cut(s) 197, 492
BmsI GCATC 2 cut(s) 27, 438
BpiI GAAGAC 1 cut(s) 428
Bpu14I TTCGAA 1 cut(s) 77
BsaBI GATNNNNATC 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 490, 491
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse21I CCTNAGG 1 cut(s) 278
Bse8I GATNNNNATC 1 cut(s) 89
BseBI CCWGG 2 cut(s) 197, 492
BseDI CCNNGG 2 cut(s) 490, 491
BseGI GGATG 1 cut(s) 429
BseJI GATNNNNATC 1 cut(s) 89
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMII CTCAG 1 cut(s) 483
BseXI GCAGC 2 cut(s) 34, 229
BshNI GGYRCC 1 cut(s) 302
BslFI GGGAC 1 cut(s) 473
BslI CCNNNNNNNGG 1 cut(s) 439
BsmFI GGGAC 1 cut(s) 473
Bsp119I TTCGAA 1 cut(s) 77
Bsp143I GATC 3 cut(s) 84, 282, 445
BspCNI CTCAG 1 cut(s) 482
BspLI GGNNCC 2 cut(s) 304, 571
BspPI GGATC 1 cut(s) 290
BspT104I TTCGAA 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 302
BspTI CTTAAG 2 cut(s) 547, 575
BssECI CCNNGG 2 cut(s) 490, 491
BssMI GATC 3 cut(s) 84, 282, 445
Bst2UI CCWGG 2 cut(s) 197, 492
Bst4CI ACNGT 3 cut(s) 188, 307, 358
BstAFI CTTAAG 2 cut(s) 547, 575
BstAPI GCANNNNNTGC 1 cut(s) 335
BstBI TTCGAA 1 cut(s) 77
BstC8I GCNNGC 3 cut(s) 27, 331, 363
BstDEI CTNAG 3 cut(s) 278, 342, 469
BstF5I GGATG 1 cut(s) 429
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 3 cut(s) 87, 285, 448
BstMBI GATC 3 cut(s) 84, 282, 445
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstNI CCWGG 2 cut(s) 197, 492
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 2 cut(s) 195, 490
BstSFI CTRYAG 1 cut(s) 513
BstV1I GCAGC 2 cut(s) 34, 229
BstV2I GAAGAC 1 cut(s) 428
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
Bsu36I CCTNAGG 1 cut(s) 278
BsuI GTATCC 1 cut(s) 157
BtsCI GGATG 1 cut(s) 429
BtsIMutI CAGTG 2 cut(s) 363, 477
Cac8I GCNNGC 3 cut(s) 27, 331, 363
CfoI GCGC 1 cut(s) 340
Csp6I GTAC 1 cut(s) 498
CviAII CATG 1 cut(s) 26
CviJI RGCY 6 cut(s) 116, 132, 200, 365, 509, 537
CviKI_1 RGCY 6 cut(s) 116, 132, 200, 365, 509, 537
CviQI GTAC 1 cut(s) 498
DdeI CTNAG 3 cut(s) 278, 342, 469
DpnI GATC 3 cut(s) 86, 284, 447
DpnII GATC 3 cut(s) 84, 282, 445
DraIII CACNNNGTG 1 cut(s) 335
DrdI GACNNNNNNGTC 1 cut(s) 449
DseDI GACNNNNNNGTC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 422
Eco81I CCTNAGG 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 195, 490
FaeI CATG 1 cut(s) 29
FaiI YATR 6 cut(s) 27, 96, 128, 140, 373, 515
FaqI GGGAC 1 cut(s) 473
FatI CATG 1 cut(s) 25
Fnu4HI GCNGC 2 cut(s) 48, 218
FokI GGATG 1 cut(s) 416
Fsp4HI GCNGC 2 cut(s) 48, 218
FspBI CTAG 2 cut(s) 30, 449
GlaI GCGC 1 cut(s) 339
GluI GCNGC 2 cut(s) 48, 218
HhaI GCGC 1 cut(s) 340
Hin1II CATG 1 cut(s) 29
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HindIII AAGCTT 2 cut(s) 363, 507
HinfI GANTC 7 cut(s) 145, 178, 192, 212, 254, 273, 314
Hpy166II GTNNAC 2 cut(s) 290, 498
Hpy188III TCNNGA 5 cut(s) 182, 206, 251, 440, 530
Hpy8I GTNNAC 2 cut(s) 290, 498
Hpy99I CGWCG 2 cut(s) 188, 429
HpyCH4III ACNGT 3 cut(s) 188, 307, 358
HpyCH4IV ACGT 1 cut(s) 500
HpyCH4V TGCA 4 cut(s) 50, 68, 329, 361
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
HpyF3I CTNAG 3 cut(s) 278, 342, 469
HpySE526I ACGT 1 cut(s) 500
Hsp92II CATG 1 cut(s) 29
HspAI GCGC 1 cut(s) 338
Kzo9I GATC 3 cut(s) 84, 282, 445
LpnPI CCDG 9 cut(s) 182, 191, 209, 236, 425, 441, 477, 504, 545
Lsp1109I GCAGC 2 cut(s) 34, 229
LweI GCATC 2 cut(s) 27, 438
MaeI CTAG 2 cut(s) 30, 449
MaeII ACGT 1 cut(s) 500
MaeIII GTNAC 1 cut(s) 451
MalI GATC 3 cut(s) 86, 284, 447
MboI GATC 3 cut(s) 84, 282, 445
MboII GAAGA 6 cut(s) 91, 94, 113, 221, 411, 428
MfeI CAATTG 1 cut(s) 324
MflI RGATCY 1 cut(s) 84
MluCI AATT 4 cut(s) 51, 221, 294, 324
MlyI GAGTC 2 cut(s) 154, 172
MnlI CCTC 2 cut(s) 258, 526
MroXI GAANNNNTTC 2 cut(s) 243, 525
MseI TTAA 4 cut(s) 71, 390, 548, 576
MspCI CTTAAG 2 cut(s) 547, 575
MspR9I CCNGG 2 cut(s) 197, 492
MunI CAATTG 1 cut(s) 324
MvaI CCWGG 2 cut(s) 197, 492
MwoI GCNNNNNNNGC 1 cut(s) 335
NdeII GATC 3 cut(s) 84, 282, 445
NlaIII CATG 1 cut(s) 29
NlaIV GGNNCC 2 cut(s) 304, 571
NmuCI GTSAC 1 cut(s) 451
NspI RCATGY 1 cut(s) 29
NspV TTCGAA 1 cut(s) 77
PaeI GCATGC 1 cut(s) 29
PasI CCCWGGG 1 cut(s) 491
PdmI GAANNNNTTC 2 cut(s) 243, 525
PfeI GAWTC 5 cut(s) 192, 212, 254, 273, 314
PkrI GCNGC 2 cut(s) 49, 219
PleI GAGTC 2 cut(s) 153, 172
PpsI GAGTC 2 cut(s) 153, 172
Psp6I CCWGG 2 cut(s) 195, 490
PspGI CCWGG 2 cut(s) 195, 490
PspN4I GGNNCC 2 cut(s) 304, 571
PsuI RGATCY 1 cut(s) 84
RsaI GTAC 1 cut(s) 499
RsaNI GTAC 1 cut(s) 498
SaqAI TTAA 4 cut(s) 71, 390, 548, 576
SatI GCNGC 2 cut(s) 48, 218
Sau3AI GATC 3 cut(s) 84, 282, 445
SchI GAGTC 2 cut(s) 154, 172
ScrFI CCNGG 2 cut(s) 197, 492
SetI ASST 5 cut(s) 118, 367, 384, 503, 511
SfaNI GCATC 2 cut(s) 27, 438
SfcI CTRYAG 1 cut(s) 513
SfuI TTCGAA 1 cut(s) 77
SmlI CTYRAG 2 cut(s) 547, 575
SmoI CTYRAG 2 cut(s) 547, 575
SphI GCATGC 1 cut(s) 29
Sse9I AATT 4 cut(s) 51, 221, 294, 324
SspMI CTAG 2 cut(s) 30, 449
StyD4I CCNGG 2 cut(s) 195, 490
TaaI ACNGT 3 cut(s) 188, 307, 358
TaiI ACGT 1 cut(s) 503
TaqI TCGA 3 cut(s) 77, 183, 529
TasI AATT 4 cut(s) 51, 221, 294, 324
TfiI GAWTC 5 cut(s) 192, 212, 254, 273, 314
Tru1I TTAA 4 cut(s) 71, 390, 548, 576
Tru9I TTAA 4 cut(s) 71, 390, 548, 576
TscAI CASTG 2 cut(s) 363, 477
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 2 cut(s) 47, 217
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 3 cut(s) 114, 276, 452
TspGWI ACGGA 1 cut(s) 422
TspRI CASTG 2 cut(s) 363, 477
Vha464I CTTAAG 2 cut(s) 547, 575
XapI RAATTY 1 cut(s) 294
XceI RCATGY 1 cut(s) 29
XmnI GAANNNNTTC 2 cut(s) 243, 525
XspI CTAG 2 cut(s) 30, 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.