RLG00000000385

NADH-ubiquinone/plastoquinone oxidoreductase chain 4L

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0019174
Physical Location & Seq
Forward (+)
782616 .. 783515
900 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000385

Sequence Viewer

Length: 636 bp
ATGGGTCAATCATTTGCTTCATTGGTTCCAACGGTGGCAGCTGCGGAATCTGCTATTGGGTTAGCCATTTTCGTTATTACTTTCCGAGTCCGAGGGACTATTGCTGTGGAATCTATTAATAGCATTCAAGGGTCAGGGCCTAGAGCGCATCCGATTCGAAAGTCCTTTCCTAAACCACTTCCCGTTCAGTTGAGTTTAGGGGATACTTTCAAAATGACTCTCGACGGTAGAATCCAGGCTATTCAGGAAGAATCGCAGCAATTCCCCAATCCTAACGAAGTAGTTCCTCTGGAATCCAATGAACAACAACGATTCCTTAGGATCAGTTTACGAATTTGTGGCACCGTAGTAGAATCATTACCAATTGCACGCTGTGCGCCTAAGTGCGAAAAGACAGTGCAAGCTTTGTTATGCCGAAACCTAAATGTTAAGTCAGCAACACTTCCAAATGCCATTTCTTCCCGTCGCATCCGTCTTCAGGACGATCTAGTCACAGGGTTTCATTTCTCAGTGAGTGAAAGATTTGTCCCTGGGTGTACGTTGAAAGCTTCTATAGTAGAACTCATTCGAGAGGGCTTGGTGGTCTTAAGAATGGTTCTGGTGGGGGGTTCCCTTAAGAATAAAGAAGACGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mt_ATP-synt_B Oxidored_q2

Protein Analysis

212

Amino Acids

23.05

Weight (kDa)

9.33

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_q2 PF00420 3 - 43 2e-06 NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
Mt_ATP-synt_B PF05405 64 - 196 2.3e-29 Mitochondrial ATP synthase B chain precursor (ATP-synt_B)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019174)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00640
rosa_chinensis RchiOBHm_MTg0498351
rosa_laevigata RLG00000000385 RLG00000000401
rosa_multiflora Rmu_sc0022700.1_g000004
rosa_roxburghii Rroxscaffold_2G00119440
rosa_samantha Rh1BG019400 Rh4BG135100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 488
AccB1I GGYRCC 1 cut(s) 341
AciI CCGC 1 cut(s) 44
AclWI GGATC 1 cut(s) 329
AcsI RAATTY 1 cut(s) 333
AcuI CTGAAG 1 cut(s) 461
AdeI CACNNNGTG 1 cut(s) 374
AfaI GTAC 1 cut(s) 538
AfiI CCNNNNNNNGG 1 cut(s) 478
AflII CTTAAG 2 cut(s) 586, 614
AgsI TTSAA 3 cut(s) 128, 211, 544
AjnI CCWGG 2 cut(s) 234, 529
AjuI GAANNNNNNNTTGG 4 cut(s) 39, 71, 439, 471
AluBI AGCT 3 cut(s) 41, 404, 548
AluI AGCT 3 cut(s) 41, 404, 548
AlwI GGATC 1 cut(s) 329
AoxI GGCC 1 cut(s) 137
ApeKI GCWGC 3 cut(s) 38, 41, 256
ApoI RAATTY 1 cut(s) 333
AseI ATTAAT 1 cut(s) 117
Asp700I GAANNNNTTC 2 cut(s) 282, 564
AspLEI GCGC 2 cut(s) 148, 379
AspS9I GGNCC 1 cut(s) 137
AsuII TTCGAA 1 cut(s) 157
AxyI CCTNAGG 1 cut(s) 317
BanI GGYRCC 1 cut(s) 341
BbsI GAAGAC 1 cut(s) 467
BbvI GCAGC 3 cut(s) 28, 50, 268
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 2 cut(s) 236, 531
BciVI GTATCC 1 cut(s) 196
BfaI CTAG 2 cut(s) 141, 488
BfmI CTRYAG 1 cut(s) 552
BfrI CTTAAG 2 cut(s) 586, 614
BfuI GTATCC 1 cut(s) 196
BisI GCNGC 3 cut(s) 39, 42, 257
BlsI GCNGC 3 cut(s) 40, 43, 258
Bme1390I CCNGG 2 cut(s) 236, 531
BmgT120I GGNCC 1 cut(s) 137
BmiI GGNNCC 3 cut(s) 27, 343, 610
BmrFI CCNGG 2 cut(s) 236, 531
BmsI GCATC 2 cut(s) 157, 477
BpiI GAAGAC 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 157
BsaJI CCNNGG 3 cut(s) 91, 529, 530
Bsc4I CCNNNNNNNGG 1 cut(s) 478
Bse21I CCTNAGG 1 cut(s) 317
BseBI CCWGG 2 cut(s) 236, 531
BseDI CCNNGG 3 cut(s) 91, 529, 530
BseGI GGATG 2 cut(s) 148, 468
BseLI CCNNNNNNNGG 1 cut(s) 478
BseMII CTCAG 1 cut(s) 522
BseXI GCAGC 3 cut(s) 28, 50, 268
BshFI GGCC 1 cut(s) 139
BshNI GGYRCC 1 cut(s) 341
BslFI GGGAC 2 cut(s) 109, 512
BslI CCNNNNNNNGG 1 cut(s) 478
BsmFI GGGAC 2 cut(s) 109, 512
BsmI GAATGC 1 cut(s) 123
BsnI GGCC 1 cut(s) 139
Bsp119I TTCGAA 1 cut(s) 157
Bsp143I GATC 2 cut(s) 321, 484
BspACI CCGC 1 cut(s) 44
BspANI GGCC 1 cut(s) 139
BspCNI CTCAG 1 cut(s) 521
BspLI GGNNCC 3 cut(s) 27, 343, 610
BspPI GGATC 1 cut(s) 329
BspT104I TTCGAA 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 341
BspTI CTTAAG 2 cut(s) 586, 614
BssECI CCNNGG 3 cut(s) 91, 529, 530
BssMI GATC 2 cut(s) 321, 484
Bst2UI CCWGG 2 cut(s) 236, 531
Bst4CI ACNGT 4 cut(s) 34, 227, 346, 397
BstAFI CTTAAG 2 cut(s) 586, 614
BstAPI GCANNNNNTGC 1 cut(s) 374
BstBI TTCGAA 1 cut(s) 157
BstC8I GCNNGC 2 cut(s) 370, 402
BstDEI CTNAG 3 cut(s) 317, 381, 508
BstF5I GGATG 2 cut(s) 148, 468
BstHHI GCGC 2 cut(s) 148, 379
BstKTI GATC 2 cut(s) 324, 487
BstMBI GATC 2 cut(s) 321, 484
BstMWI GCNNNNNNNGC 3 cut(s) 50, 145, 374
BstNI CCWGG 2 cut(s) 236, 531
BstSCI CCNGG 2 cut(s) 234, 529
BstSFI CTRYAG 1 cut(s) 552
BstV1I GCAGC 3 cut(s) 28, 50, 268
BstV2I GAAGAC 1 cut(s) 467
Bsu36I CCTNAGG 1 cut(s) 317
BsuI GTATCC 1 cut(s) 196
BsuRI GGCC 1 cut(s) 139
BtsCI GGATG 2 cut(s) 148, 468
BtsIMutI CAGTG 2 cut(s) 402, 516
Cac8I GCNNGC 2 cut(s) 370, 402
CfoI GCGC 2 cut(s) 148, 379
Cfr13I GGNCC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 537
CviJI RGCY 7 cut(s) 41, 65, 139, 239, 404, 548, 576
CviKI_1 RGCY 7 cut(s) 41, 65, 139, 239, 404, 548, 576
CviQI GTAC 1 cut(s) 537
DdeI CTNAG 3 cut(s) 317, 381, 508
DpnI GATC 2 cut(s) 323, 486
DpnII GATC 2 cut(s) 321, 484
DraIII CACNNNGTG 1 cut(s) 374
DrdI GACNNNNNNGTC 1 cut(s) 488
DseDI GACNNNNNNGTC 1 cut(s) 488
Eco57I CTGAAG 1 cut(s) 461
Eco81I CCTNAGG 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 137
EcoRII CCWGG 2 cut(s) 234, 529
FaiI YATR 2 cut(s) 412, 554
FaqI GGGAC 2 cut(s) 109, 512
Fnu4HI GCNGC 3 cut(s) 39, 42, 257
FokI GGATG 2 cut(s) 135, 455
Fsp4HI GCNGC 3 cut(s) 39, 42, 257
FspBI CTAG 2 cut(s) 141, 488
GlaI GCGC 2 cut(s) 147, 378
GluI GCNGC 3 cut(s) 39, 42, 257
HaeIII GGCC 1 cut(s) 139
HhaI GCGC 2 cut(s) 148, 379
Hin6I GCGC 2 cut(s) 146, 377
HinP1I GCGC 2 cut(s) 146, 377
HindIII AAGCTT 2 cut(s) 402, 546
Hpy166II GTNNAC 2 cut(s) 329, 537
Hpy188I TCNGA 3 cut(s) 86, 92, 153
Hpy188III TCNNGA 5 cut(s) 221, 245, 290, 479, 569
Hpy8I GTNNAC 2 cut(s) 329, 537
Hpy99I CGWCG 2 cut(s) 227, 468
HpyCH4III ACNGT 4 cut(s) 34, 227, 346, 397
HpyCH4IV ACGT 1 cut(s) 539
HpyCH4V TGCA 2 cut(s) 368, 400
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 145, 374
HpyF3I CTNAG 3 cut(s) 317, 381, 508
HpySE526I ACGT 1 cut(s) 539
HspAI GCGC 2 cut(s) 146, 377
Kzo9I GATC 2 cut(s) 321, 484
Lsp1109I GCAGC 3 cut(s) 28, 50, 268
LweI GCATC 2 cut(s) 157, 477
MaeI CTAG 2 cut(s) 141, 488
MaeII ACGT 1 cut(s) 539
MaeIII GTNAC 1 cut(s) 490
MalI GATC 2 cut(s) 323, 486
MboI GATC 2 cut(s) 321, 484
MboII GAAGA 3 cut(s) 260, 450, 467
MfeI CAATTG 1 cut(s) 363
MluCI AATT 3 cut(s) 260, 333, 363
MlyI GAGTC 2 cut(s) 96, 211
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 3 cut(s) 86, 297, 565
MroXI GAANNNNTTC 2 cut(s) 282, 564
MseI TTAA 4 cut(s) 117, 429, 587, 615
MspA1I CMGCKG 1 cut(s) 41
MspCI CTTAAG 2 cut(s) 586, 614
MspR9I CCNGG 2 cut(s) 236, 531
MunI CAATTG 1 cut(s) 363
Mva1269I GAATGC 1 cut(s) 123
MvaI CCWGG 2 cut(s) 236, 531
MwoI GCNNNNNNNGC 3 cut(s) 50, 145, 374
NdeII GATC 2 cut(s) 321, 484
NlaIV GGNNCC 3 cut(s) 27, 343, 610
NmuCI GTSAC 1 cut(s) 490
NspV TTCGAA 1 cut(s) 157
PasI CCCWGGG 1 cut(s) 530
PctI GAATGC 1 cut(s) 123
PdmI GAANNNNTTC 2 cut(s) 282, 564
PfeI GAWTC 8 cut(s) 47, 110, 154, 231, 251, 293, 312, 353
PkrI GCNGC 3 cut(s) 40, 43, 258
PleI GAGTC 2 cut(s) 95, 211
PpsI GAGTC 2 cut(s) 95, 211
PshBI ATTAAT 1 cut(s) 117
Psp6I CCWGG 2 cut(s) 234, 529
PspGI CCWGG 2 cut(s) 234, 529
PspN4I GGNNCC 3 cut(s) 27, 343, 610
PspPI GGNCC 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 1 cut(s) 538
RsaNI GTAC 1 cut(s) 537
SaqAI TTAA 4 cut(s) 117, 429, 587, 615
SatI GCNGC 3 cut(s) 39, 42, 257
Sau3AI GATC 2 cut(s) 321, 484
Sau96I GGNCC 1 cut(s) 137
SchI GAGTC 2 cut(s) 96, 211
ScrFI CCNGG 2 cut(s) 236, 531
SetI ASST 5 cut(s) 43, 406, 423, 542, 550
SfaNI GCATC 2 cut(s) 157, 477
SfcI CTRYAG 1 cut(s) 552
SfuI TTCGAA 1 cut(s) 157
SmlI CTYRAG 2 cut(s) 586, 614
SmoI CTYRAG 2 cut(s) 586, 614
Sse9I AATT 3 cut(s) 260, 333, 363
SsiI CCGC 1 cut(s) 44
SspMI CTAG 2 cut(s) 141, 488
StyD4I CCNGG 2 cut(s) 234, 529
TaaI ACNGT 4 cut(s) 34, 227, 346, 397
TaiI ACGT 1 cut(s) 542
TaqI TCGA 3 cut(s) 157, 222, 568
TasI AATT 3 cut(s) 260, 333, 363
TfiI GAWTC 8 cut(s) 47, 110, 154, 231, 251, 293, 312, 353
Tru1I TTAA 4 cut(s) 117, 429, 587, 615
Tru9I TTAA 4 cut(s) 117, 429, 587, 615
TscAI CASTG 2 cut(s) 402, 516
TseFI GTSAC 1 cut(s) 490
TseI GCWGC 3 cut(s) 38, 41, 256
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 3 cut(s) 9, 315, 491
TspGWI ACGGA 1 cut(s) 461
TspRI CASTG 2 cut(s) 402, 516
Vha464I CTTAAG 2 cut(s) 586, 614
VspI ATTAAT 1 cut(s) 117
XapI RAATTY 1 cut(s) 333
XmnI GAANNNNTTC 2 cut(s) 282, 564
XspI CTAG 2 cut(s) 141, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.