RLG00000003011

protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017119
Physical Location & Seq
Reverse (-)
43022977 .. 43024035
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003011

Sequence Viewer

Length: 1059 bp
ATGACATTAGTGAGAGAGAGAAGGCATCAGCAAGGACTAAGGCTCTCTCCACCGCCACCACCAGTGACTGCAACAGCTGACTTCACCCACCGGAGCTTTTTTCCGGCACTGTCCCCAGCCTCCAGTCCTGACTCTCCAGCCATTATTGAAAACCTCTCCGATCTCGAGAAAGTTGAGGTTCTTGGCCACGGGGATGGCGGCACTGTCTATAAAGTACGTCACAAGAAAAGCTCATGCATTTACGCCTTGAAAGTTCTGCGGTTCGACAACAATGCCGCCACCGGTATCTTGCAACAGGTGGCGCGTGAGGCGGATATCCTCAAACTGGTTGACTCGCCCTACGTCATAAGATGTCATGGGGTTTTCGACAATGGGGCGTTCATGATCAGTCCGGATCATAGTAATAATGAGGGTGGCGGAGATTTGTGTTTTGTAATGGAGTACATGGAAGGAGGCTCACTGCACGATATACTGCAAGCACGTCAGAGATTACCCGAGCAGCTAATCTCTCGCGTGGCAAAATGTGTCCTCCAAGGACTGCGCTATCTACATGCCATGCAGATAGTGCATAGGGACATAAAGCCTTCAAACCTCCTCATAAACGGTAGAGGGGAGATAAAGATTGCAGATTTTGGGGTCAGTCACATGGTTGCGGGTGCCCATGAGGCATGTGACTTGCACATGGGTACGTACGCTTACATGAGCCCGGAGAGATTTGATCCAGAGAGGTGGGGCGGCCGCAATGCAGATGGTTTTGCTGGAGATGTGTGGTCACTTGGATTAGTGGTGTTGCAATGCTACGTTGGCCGGTTTCCGCTCATTGGACCGGGGCAGAAACCGGACTGGCCGACGTTGATGTGTGTGATTTGCTTTGGGGAGAGGCTAGAGATGCCGGAAACGGCCTCGCCGGAGTTCCGGAGTTTCATTTGGAGGTGTCTTGAGAAGGATTGGAGGAAGAGAGCAAAAGTTGATGAGCTTCTTGACCACCCTTTTGTGAATAAAACTTGTTGCGCTTCTACCGATCAAGAGCTCGTTAATTTTGTTTTCCCTGTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000187 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009966 GO:0009967 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010646 GO:0010647 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

353

Amino Acids

39.36

Weight (kDa)

6.23

Isoelectric Point (pI)

54.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 56 - 332 5.5e-60 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 56 - 327 2.5e-32 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 144 - 217 4.1e-08 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017119)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32320
fragaria_vesca FvH4_5g22730
prunus_persica Prupe.5G031000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0211301
rosa_laevigata RLG00000003011
rosa_multiflora Rmu_sc0000006.1_g000001
rosa_roxburghii Rroxscaffold_3G00247940
rosa_rugosa Rorug07G0124900
rosa_samantha Rh7AG257300 Rh7BG251400 Rh7CG274100 Rh7DG264500
rosa_wichuraiana Rw7G021990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 656
AccBSI CCGCTC 1 cut(s) 817
AccII CGCG 2 cut(s) 304, 513
AccIII TCCGGA 2 cut(s) 391, 915
AclWI GGATC 2 cut(s) 402, 713
AcoI YGGCCR 4 cut(s) 184, 736, 805, 845
AfaI GTAC 4 cut(s) 216, 443, 688, 692
AfiI CCNNNNNNNGG 2 cut(s) 325, 821
AgeI ACCGGT 1 cut(s) 281
AgsI TTSAA 3 cut(s) 149, 250, 588
AjiI CACGTC 1 cut(s) 482
AluBI AGCT 6 cut(s) 77, 96, 231, 502, 976, 1030
AluI AGCT 6 cut(s) 77, 96, 231, 502, 976, 1030
Alw21I GWGCWC 1 cut(s) 1032
AlwI GGATC 2 cut(s) 402, 713
AlwNI CAGNNNCTG 1 cut(s) 68
Ama87I CYCGRG 2 cut(s) 164, 494
Aor13HI TCCGGA 2 cut(s) 391, 915
AoxI GGCC 5 cut(s) 184, 736, 805, 845, 900
ApeKI GCWGC 1 cut(s) 499
ArsI GACNNNNNNTTYG 2 cut(s) 974, 1006
AsiGI ACCGGT 1 cut(s) 281
AspLEI GCGC 3 cut(s) 304, 543, 1013
AspS9I GGNCC 1 cut(s) 824
AsuC2I CCSGG 2 cut(s) 707, 828
AsuHPI GGTGA 1 cut(s) 76
AvaI CYCGRG 2 cut(s) 164, 494
AvaII GGWCC 1 cut(s) 824
BaeGI GKGCMC 1 cut(s) 661
BalI TGGCCA 1 cut(s) 186
BanI GGYRCC 1 cut(s) 656
BanII GRGCYC 2 cut(s) 707, 1032
Bbv12I GWGCWC 1 cut(s) 1032
BbvI GCAGC 1 cut(s) 511
BccI CCATC 2 cut(s) 188, 743
BceAI ACGGC 1 cut(s) 915
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BclI TGATCA 1 cut(s) 384
BcnI CCSGG 2 cut(s) 707, 828
BfaI CTAG 1 cut(s) 884
BglI GCCNNNNNGGC 1 cut(s) 665
BisI GCNGC 5 cut(s) 199, 276, 500, 736, 739
BlsI GCNGC 5 cut(s) 200, 277, 501, 737, 740
Bme1390I CCNGG 2 cut(s) 707, 828
Bme18I GGWCC 1 cut(s) 824
BmeT110I CYCGRG 2 cut(s) 164, 494
BmgBI CACGTC 1 cut(s) 482
BmgT120I GGNCC 1 cut(s) 824
BmiI GGNNCC 1 cut(s) 658
BmrFI CCNGG 2 cut(s) 707, 828
BmsI GCATC 2 cut(s) 34, 879
BpmI CTGGAG 3 cut(s) 106, 120, 780
BpuEI CTTGAG 1 cut(s) 959
BpuMI CCSGG 2 cut(s) 707, 828
BsaAI YACGTR 1 cut(s) 690
BsaJI CCNNGG 3 cut(s) 187, 532, 827
BsaWI WCCGGW 5 cut(s) 90, 281, 391, 838, 915
Bsc4I CCNNNNNNNGG 2 cut(s) 325, 821
Bse118I RCCGGY 2 cut(s) 281, 807
Bse1I ACTGG 4 cut(s) 62, 123, 330, 848
Bse3DI GCAATG 2 cut(s) 748, 800
BseAI TCCGGA 2 cut(s) 391, 915
BseDI CCNNGG 3 cut(s) 187, 532, 827
BseGI GGATG 1 cut(s) 199
BseLI CCNNNNNNNGG 2 cut(s) 325, 821
BseMI GCAATG 2 cut(s) 748, 800
BseNI ACTGG 4 cut(s) 62, 123, 330, 848
BseRI GAGGAG 1 cut(s) 584
BseSI GKGCMC 1 cut(s) 661
BseX3I CGGCCG 1 cut(s) 736
BseXI GCAGC 1 cut(s) 511
BseYI CCCAGC 1 cut(s) 115
BsgI GTGCAG 1 cut(s) 446
Bsh1236I CGCG 2 cut(s) 304, 513
Bsh1285I CGRYCG 1 cut(s) 739
BshFI GGCC 5 cut(s) 186, 738, 807, 847, 902
BshNI GGYRCC 1 cut(s) 656
BshTI ACCGGT 1 cut(s) 281
BsiEI CGRYCG 1 cut(s) 739
BsiHKAI GWGCWC 1 cut(s) 1032
BsiHKCI CYCGRG 2 cut(s) 164, 494
BsiWI CGTACG 1 cut(s) 690
BslFI GGGAC 2 cut(s) 97, 587
BslI CCNNNNNNNGG 2 cut(s) 325, 821
BsmFI GGGAC 2 cut(s) 97, 587
BsnI GGCC 5 cut(s) 186, 738, 807, 847, 902
BsoBI CYCGRG 2 cut(s) 164, 494
Bsp1286I GDGCHC 3 cut(s) 661, 707, 1032
Bsp13I TCCGGA 2 cut(s) 391, 915
Bsp143I GATC 5 cut(s) 160, 384, 394, 718, 1021
BspANI GGCC 5 cut(s) 186, 738, 807, 847, 902
BspEI TCCGGA 2 cut(s) 391, 915
BspFNI CGCG 2 cut(s) 304, 513
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 1 cut(s) 658
BspPI GGATC 2 cut(s) 402, 713
BspT107I GGYRCC 1 cut(s) 656
BsrBI CCGCTC 1 cut(s) 817
BsrDI GCAATG 2 cut(s) 748, 800
BsrFI RCCGGY 2 cut(s) 281, 807
BsrI ACTGG 4 cut(s) 62, 123, 330, 848
BssAI RCCGGY 2 cut(s) 281, 807
BssECI CCNNGG 3 cut(s) 187, 532, 827
BssMI GATC 5 cut(s) 160, 384, 394, 718, 1021
BssT1I CCWWGG 1 cut(s) 532
Bst4CI ACNGT 3 cut(s) 111, 205, 605
Bst6I CTCTTC 1 cut(s) 950
BstAPI GCANNNNNTGC 1 cut(s) 565
BstBAI YACGTR 1 cut(s) 690
BstC8I GCNNGC 1 cut(s) 477
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 1 cut(s) 187
BstF5I GGATG 1 cut(s) 199
BstFNI CGCG 2 cut(s) 304, 513
BstHHI GCGC 3 cut(s) 304, 543, 1013
BstKTI GATC 5 cut(s) 163, 387, 397, 721, 1024
BstMBI GATC 5 cut(s) 160, 384, 394, 718, 1021
BstMCI CGRYCG 1 cut(s) 739
BstMWI GCNNNNNNNGC 5 cut(s) 308, 565, 665, 804, 889
BstNSI RCATGY 2 cut(s) 554, 672
BstSCI CCNGG 2 cut(s) 705, 826
BstSLI GKGCMC 1 cut(s) 661
BstSNI TACGTA 1 cut(s) 690
BstUI CGCG 2 cut(s) 304, 513
BstV1I GCAGC 1 cut(s) 511
BstXI CCANNNNNNTGG 2 cut(s) 194, 729
BstZI CGGCCG 1 cut(s) 736
BsuRI GGCC 5 cut(s) 186, 738, 807, 847, 902
BtgI CCRYGG 1 cut(s) 187
BtrI CACGTC 1 cut(s) 482
BtsCI GGATG 1 cut(s) 199
BtsI GCAGTG 1 cut(s) 458
BtsIMutI CAGTG 4 cut(s) 69, 107, 201, 458
Cac8I GCNNGC 1 cut(s) 477
CaiI CAGNNNCTG 1 cut(s) 68
CciI TCATGA 1 cut(s) 381
CciNI GCGGCCGC 1 cut(s) 736
CfoI GCGC 3 cut(s) 304, 543, 1013
Cfr10I RCCGGY 2 cut(s) 281, 807
Cfr13I GGNCC 1 cut(s) 824
Csp6I GTAC 4 cut(s) 215, 442, 687, 691
CspAI ACCGGT 1 cut(s) 281
CviQI GTAC 4 cut(s) 215, 442, 687, 691
DdeI CTNAG 1 cut(s) 38
DpnI GATC 5 cut(s) 162, 386, 396, 720, 1023
DpnII GATC 5 cut(s) 160, 384, 394, 718, 1021
EaeI YGGCCR 4 cut(s) 184, 736, 805, 845
EagI CGGCCG 1 cut(s) 736
Eam1104I CTCTTC 1 cut(s) 950
EarI CTCTTC 1 cut(s) 950
EciI GGCGGA 2 cut(s) 326, 432
Ecl136II GAGCTC 1 cut(s) 1030
EclXI CGGCCG 1 cut(s) 736
Eco105I TACGTA 1 cut(s) 690
Eco130I CCWWGG 1 cut(s) 532
Eco24I GRGCYC 2 cut(s) 707, 1032
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 824
Eco52I CGGCCG 1 cut(s) 736
Eco53kI GAGCTC 1 cut(s) 1030
Eco88I CYCGRG 2 cut(s) 164, 494
EcoICRI GAGCTC 1 cut(s) 1030
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 1 cut(s) 532
EcoT22I ATGCAT 1 cut(s) 239
EcoT38I GRGCYC 2 cut(s) 707, 1032
ErhI CCWWGG 1 cut(s) 532
FaqI GGGAC 2 cut(s) 97, 587
FauI CCCGC 1 cut(s) 646
FbaI TGATCA 1 cut(s) 384
Fnu4HI GCNGC 5 cut(s) 199, 276, 500, 736, 739
FokI GGATG 1 cut(s) 206
FriOI GRGCYC 2 cut(s) 707, 1032
Fsp4HI GCNGC 5 cut(s) 199, 276, 500, 736, 739
FspBI CTAG 1 cut(s) 884
GlaI GCGC 3 cut(s) 303, 542, 1012
GluI GCNGC 5 cut(s) 199, 276, 500, 736, 739
GsaI CCCAGC 1 cut(s) 119
GsuI CTGGAG 3 cut(s) 106, 120, 780
HaeIII GGCC 5 cut(s) 186, 738, 807, 847, 902
HhaI GCGC 3 cut(s) 304, 543, 1013
Hin6I GCGC 3 cut(s) 302, 541, 1011
HinP1I GCGC 3 cut(s) 302, 541, 1011
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 2 cut(s) 131, 332
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 2 cut(s) 160, 486
Hpy8I GTNNAC 1 cut(s) 331
Hpy99I CGWCG 1 cut(s) 853
HpyAV CCTTC 4 cut(s) 15, 443, 594, 937
HpyCH4III ACNGT 3 cut(s) 111, 205, 605
HpyCH4IV ACGT 6 cut(s) 217, 342, 481, 689, 801, 851
HpyF10VI GCNNNNNNNGC 5 cut(s) 308, 565, 665, 804, 889
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 6 cut(s) 217, 342, 481, 689, 801, 851
HspAI GCGC 3 cut(s) 302, 541, 1011
Kpn2I TCCGGA 2 cut(s) 391, 915
Ksp22I TGATCA 1 cut(s) 384
Kzo9I GATC 5 cut(s) 160, 384, 394, 718, 1021
LmnI GCTCC 1 cut(s) 93
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 2 cut(s) 34, 879
MaeI CTAG 1 cut(s) 884
MaeII ACGT 6 cut(s) 217, 342, 481, 689, 801, 851
MaeIII GTNAC 5 cut(s) 64, 218, 641, 671, 771
MalI GATC 5 cut(s) 162, 386, 396, 720, 1023
MbiI CCGCTC 1 cut(s) 817
MboI GATC 5 cut(s) 160, 384, 394, 718, 1021
MboII GAAGA 1 cut(s) 967
MhlI GDGCHC 3 cut(s) 661, 707, 1032
MlsI TGGCCA 1 cut(s) 186
MluCI AATT 1 cut(s) 1036
MluNI TGGCCA 1 cut(s) 186
MlyI GAGTC 2 cut(s) 125, 326
Mox20I TGGCCA 1 cut(s) 186
Mph1103I ATGCAT 1 cut(s) 239
MroI TCCGGA 2 cut(s) 391, 915
MscI TGGCCA 1 cut(s) 186
MseI TTAA 1 cut(s) 1035
MslI CAYNNNNRTG 1 cut(s) 192
Msp20I TGGCCA 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 77
MspR9I CCNGG 2 cut(s) 707, 828
MvnI CGCG 2 cut(s) 304, 513
MwoI GCNNNNNNNGC 5 cut(s) 308, 565, 665, 804, 889
NciI CCSGG 2 cut(s) 707, 828
NdeII GATC 5 cut(s) 160, 384, 394, 718, 1021
NlaIV GGNNCC 1 cut(s) 658
NmuCI GTSAC 5 cut(s) 64, 218, 641, 671, 771
NotI GCGGCCGC 1 cut(s) 736
NsiI ATGCAT 1 cut(s) 239
NspI RCATGY 2 cut(s) 554, 672
PaeR7I CTCGAG 1 cut(s) 164
PagI TCATGA 1 cut(s) 381
Pfl23II CGTACG 1 cut(s) 690
PinAI ACCGGT 1 cut(s) 281
PkrI GCNGC 5 cut(s) 200, 277, 501, 737, 740
PleI GAGTC 2 cut(s) 125, 326
PpsI GAGTC 2 cut(s) 125, 326
Ppu21I YACGTR 1 cut(s) 690
Psp124BI GAGCTC 1 cut(s) 1032
PspFI CCCAGC 1 cut(s) 115
PspLI CGTACG 1 cut(s) 690
PspN4I GGNNCC 1 cut(s) 658
PspPI GGNCC 1 cut(s) 824
PstNI CAGNNNCTG 1 cut(s) 68
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 4 cut(s) 216, 443, 688, 692
RsaNI GTAC 4 cut(s) 215, 442, 687, 691
RseI CAYNNNNRTG 1 cut(s) 192
SacI GAGCTC 1 cut(s) 1032
SaqAI TTAA 1 cut(s) 1035
SatI GCNGC 5 cut(s) 199, 276, 500, 736, 739
Sau3AI GATC 5 cut(s) 160, 384, 394, 718, 1021
Sau96I GGNCC 1 cut(s) 824
SchI GAGTC 2 cut(s) 125, 326
ScrFI CCNGG 2 cut(s) 707, 828
SduI GDGCHC 3 cut(s) 661, 707, 1032
SfaNI GCATC 2 cut(s) 34, 879
Sfr274I CTCGAG 1 cut(s) 164
SinI GGWCC 1 cut(s) 824
SlaI CTCGAG 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 192
SmlI CTYRAG 2 cut(s) 164, 938
SmoI CTYRAG 2 cut(s) 164, 938
SnaBI TACGTA 1 cut(s) 690
Sse9I AATT 1 cut(s) 1036
SspMI CTAG 1 cut(s) 884
SstI GAGCTC 1 cut(s) 1032
StyD4I CCNGG 2 cut(s) 705, 826
StyI CCWWGG 1 cut(s) 532
TaaI ACNGT 3 cut(s) 111, 205, 605
TaiI ACGT 6 cut(s) 220, 345, 484, 692, 804, 854
TaqI TCGA 3 cut(s) 165, 264, 366
TasI AATT 1 cut(s) 1036
TatI WGTACW 1 cut(s) 441
TauI GCSGC 4 cut(s) 201, 278, 738, 741
Tru1I TTAA 1 cut(s) 1035
Tru9I TTAA 1 cut(s) 1035
TscAI CASTG 4 cut(s) 69, 114, 208, 465
TseFI GTSAC 5 cut(s) 64, 218, 641, 671, 771
TseI GCWGC 1 cut(s) 499
Tsp45I GTSAC 5 cut(s) 64, 218, 641, 671, 771
TspDTI ATGAA 2 cut(s) 370, 913
TspRI CASTG 4 cut(s) 69, 114, 208, 465
VpaK11BI GGWCC 1 cut(s) 824
XceI RCATGY 2 cut(s) 554, 672
XhoI CTCGAG 1 cut(s) 164
XspI CTAG 1 cut(s) 884
Zsp2I ATGCAT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.