Rw7G021990

protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0017119
Physical Location & Seq
Forward (+)
25660045 .. 25662547
2503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G021990.1

Sequence Viewer

Length: 1128 bp
ATGACATTAGTGAGAGAGAGAAGGCATCAGCAAGGACTAAGGCTCTCTCCACCGCCACCACCAGTGACTGCAACAGCTGACTTCACCCACCGGAGCTTTTTTCCGGCACTGTCCCCAGCCTCCAGTCCTGACTCTCCAGCCATTATTGAAAGCCTCTCCGATCTCGAGAAAGTTGAGGTTCTTGGCCACGGGGATGGCGGCACTGTCTATAAAGTACGTCACAAGAAAAGCTCATGCATTTACGCCTTGAAAGTTCTGCGGTTCGACAACAATGCCGCCACCGGTATCTTGCAACAGGTGGCGCGTGAGGCGGAGATCCTGAAACTGGTTGACTCACCCTACGTTATAAGATGTCATGGGGTTTTTGACAGTGGGGCGTTCATGAGTCCGGATCATAGTAATAATGAGGGTGGAGGAGATTTGTGTTTTGTAATGGAGTACATGGAAGGAGGCTCACTGCACGATGTACTGCGAGCACGTCAGAGATTACCCGAGCAGCTAATCTCTCGCGTGGCAAAATGTGTCCTCCAAGGACTGCGCTATCTACATGCCATGCAGATAGTGCATAGGGACATAAAGCCTTCAAACCTCCTCATAAACGGTAGAGGGGAGATAAAGATTGCAGATTTTGGGGTCAGTCACATGGTTGCGGGTGCCCATGAGGCATGTGACTTGCACATGGGTACGTACGCTTACATGAGCCCGGAGAGATTTGATCCAGAGAGGTGGGGCGGCCGCAATGCAGATGGTTTTGCTGGAGATGTGTGGTCACTTGGATTGGTGGTGTTGCAATGCTACGTTGGCAGGTTTCCGCTAATTGGACCGGGGCAGAAACCTGACTGGCCGACGTTGATGTGTGTGATTTGCTTTGGAGATCAGAGGCTGGAGATGCCGGAAACGGCCTCGCCAGAGTTCCGGAGTTTCATTTGGAGGTGTCTTGAGAAGGATTGGAGGAAGAGAGCAAAAGTTGATGAGCTTCTTGACCACCCTTTTGCCTCGACCTCAGGACAACAAACCCAAGGGAAAGGTTGTTGTTCATCTTATTCAAGACCAAGATTACTATGCGGTGACAAAACCAGATCACACCAACTTATTCAAGGATACCGCATCCGTAAGTCCCAGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000187 GO:0001932 GO:0001934 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006521 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0009605 GO:0009607 GO:0009626 GO:0009791 GO:0009814 GO:0009838 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009966 GO:0009967 GO:0009987 GO:0010227 GO:0010229 GO:0010364 GO:0010365 GO:0010562 GO:0010565 GO:0010604 GO:0010646 GO:0010647 GO:0010817 GO:0012501 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031335 GO:0031337 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0033238 GO:0033240 GO:0033554 GO:0033674 GO:0034050 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042762 GO:0043085 GO:0043170 GO:0043207 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0045764 GO:0045859 GO:0045860 GO:0045937 GO:0046885 GO:0046886 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051176 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1900908 GO:1900910 GO:1900911 GO:1900913 GO:1901564 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

375

Amino Acids

41.97

Weight (kDa)

8.55

Isoelectric Point (pI)

55.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 56 - 331 1.1e-59 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 56 - 327 3.1e-32 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 143 - 216 5.1e-08 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017119)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32320
fragaria_vesca FvH4_5g22730
prunus_persica Prupe.5G031000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0211301
rosa_laevigata RLG00000003011
rosa_multiflora Rmu_sc0000006.1_g000001
rosa_roxburghii Rroxscaffold_3G00247940
rosa_rugosa Rorug07G0124900
rosa_samantha Rh7AG257300 Rh7BG251400 Rh7CG274100 Rh7DG264500
rosa_wichuraiana Rw7G021990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 347
Acc36I ACCTGC 1 cut(s) 795
AccB1I GGYRCC 1 cut(s) 653
AccII CGCG 2 cut(s) 304, 510
AccIII TCCGGA 2 cut(s) 388, 915
AclWI GGATC 3 cut(s) 310, 399, 710
AcoI YGGCCR 3 cut(s) 184, 733, 842
AfaI GTAC 5 cut(s) 216, 440, 468, 685, 689
AfiI CCNNNNNNNGG 2 cut(s) 325, 818
AgeI ACCGGT 1 cut(s) 281
AgsI TTSAA 5 cut(s) 149, 250, 585, 1047, 1097
AjiI CACGTC 1 cut(s) 479
AluBI AGCT 5 cut(s) 77, 96, 231, 499, 976
AluI AGCT 5 cut(s) 77, 96, 231, 499, 976
Alw21I GWGCWC 1 cut(s) 478
AlwI GGATC 3 cut(s) 310, 399, 710
AlwNI CAGNNNCTG 2 cut(s) 68, 883
Ama87I CYCGRG 2 cut(s) 164, 491
Aor13HI TCCGGA 2 cut(s) 388, 915
AoxI GGCC 4 cut(s) 184, 733, 842, 900
ApeKI GCWGC 1 cut(s) 496
ArsI GACNNNNNNTTYG 2 cut(s) 974, 1006
AsiGI ACCGGT 1 cut(s) 281
AspLEI GCGC 2 cut(s) 304, 540
AspS9I GGNCC 1 cut(s) 821
AsuC2I CCSGG 2 cut(s) 704, 825
AsuHPI GGTGA 3 cut(s) 76, 327, 1079
AvaI CYCGRG 2 cut(s) 164, 491
AvaII GGWCC 1 cut(s) 821
AxyI CCTNAGG 1 cut(s) 1003
BaeGI GKGCMC 1 cut(s) 658
BalI TGGCCA 1 cut(s) 186
BanI GGYRCC 1 cut(s) 653
BanII GRGCYC 1 cut(s) 704
Bbv12I GWGCWC 1 cut(s) 478
BbvI GCAGC 1 cut(s) 508
BccI CCATC 2 cut(s) 188, 740
BceAI ACGGC 1 cut(s) 915
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciVI GTATCC 1 cut(s) 1094
BcnI CCSGG 2 cut(s) 704, 825
BfuAI ACCTGC 1 cut(s) 795
BfuI GTATCC 1 cut(s) 1094
BglI GCCNNNNNGGC 1 cut(s) 662
BisI GCNGC 5 cut(s) 199, 276, 497, 733, 736
BlsI GCNGC 5 cut(s) 200, 277, 498, 734, 737
Bme1390I CCNGG 2 cut(s) 704, 825
Bme18I GGWCC 1 cut(s) 821
BmeT110I CYCGRG 2 cut(s) 164, 491
BmgBI CACGTC 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 821
BmiI GGNNCC 1 cut(s) 655
BmrFI CCNGG 2 cut(s) 704, 825
BmrI ACTGGG 1 cut(s) 1114
BmsI GCATC 3 cut(s) 34, 879, 1116
BmuI ACTGGG 1 cut(s) 1114
BpmI CTGGAG 4 cut(s) 106, 120, 777, 905
BpuEI CTTGAG 1 cut(s) 959
BpuMI CCSGG 2 cut(s) 704, 825
BsaAI YACGTR 1 cut(s) 687
BsaJI CCNNGG 4 cut(s) 187, 529, 824, 1018
BsaWI WCCGGW 4 cut(s) 90, 281, 388, 915
Bsc4I CCNNNNNNNGG 2 cut(s) 325, 818
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 5 cut(s) 62, 123, 330, 845, 1120
Bse21I CCTNAGG 1 cut(s) 1003
Bse3DI GCAATG 2 cut(s) 745, 797
BseAI TCCGGA 2 cut(s) 388, 915
BseDI CCNNGG 4 cut(s) 187, 529, 824, 1018
BseGI GGATG 2 cut(s) 199, 1107
BseLI CCNNNNNNNGG 2 cut(s) 325, 818
BseMI GCAATG 2 cut(s) 745, 797
BseMII CTCAG 1 cut(s) 1017
BseNI ACTGG 5 cut(s) 62, 123, 330, 845, 1120
BseRI GAGGAG 2 cut(s) 429, 581
BseSI GKGCMC 1 cut(s) 658
BseX3I CGGCCG 1 cut(s) 733
BseXI GCAGC 1 cut(s) 508
BseYI CCCAGC 1 cut(s) 115
BsgI GTGCAG 1 cut(s) 443
Bsh1236I CGCG 2 cut(s) 304, 510
Bsh1285I CGRYCG 1 cut(s) 736
BshFI GGCC 4 cut(s) 186, 735, 844, 902
BshNI GGYRCC 1 cut(s) 653
BshTI ACCGGT 1 cut(s) 281
BsiEI CGRYCG 1 cut(s) 736
BsiHKAI GWGCWC 1 cut(s) 478
BsiHKCI CYCGRG 2 cut(s) 164, 491
BsiSI CCGG 8 cut(s) 91, 104, 282, 389, 704, 824, 893, 916
BsiWI CGTACG 1 cut(s) 687
BslFI GGGAC 3 cut(s) 97, 584, 1102
BslI CCNNNNNNNGG 2 cut(s) 325, 818
BsmFI GGGAC 3 cut(s) 97, 584, 1102
BsnI GGCC 4 cut(s) 186, 735, 844, 902
BsoBI CYCGRG 2 cut(s) 164, 491
Bsp1286I GDGCHC 3 cut(s) 478, 658, 704
Bsp13I TCCGGA 2 cut(s) 388, 915
Bsp143I GATC 6 cut(s) 160, 315, 391, 715, 874, 1079
BspANI GGCC 4 cut(s) 186, 735, 844, 902
BspCNI CTCAG 1 cut(s) 1016
BspEI TCCGGA 2 cut(s) 388, 915
BspFNI CGCG 2 cut(s) 304, 510
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 1 cut(s) 655
BspMI ACCTGC 1 cut(s) 795
BspPI GGATC 3 cut(s) 310, 399, 710
BspT107I GGYRCC 1 cut(s) 653
BsrDI GCAATG 2 cut(s) 745, 797
BsrFI RCCGGY 1 cut(s) 281
BsrI ACTGG 5 cut(s) 62, 123, 330, 845, 1120
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 4 cut(s) 187, 529, 824, 1018
BssMI GATC 6 cut(s) 160, 315, 391, 715, 874, 1079
BssT1I CCWWGG 2 cut(s) 529, 1018
Bst4CI ACNGT 4 cut(s) 111, 205, 371, 602
Bst6I CTCTTC 1 cut(s) 950
BstAPI GCANNNNNTGC 1 cut(s) 562
BstBAI YACGTR 1 cut(s) 687
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 2 cut(s) 38, 1003
BstDSI CCRYGG 1 cut(s) 187
BstF5I GGATG 2 cut(s) 199, 1107
BstFNI CGCG 2 cut(s) 304, 510
BstHHI GCGC 2 cut(s) 304, 540
BstKTI GATC 6 cut(s) 163, 318, 394, 718, 877, 1082
BstMBI GATC 6 cut(s) 160, 315, 391, 715, 874, 1079
BstMCI CGRYCG 1 cut(s) 736
BstMWI GCNNNNNNNGC 5 cut(s) 308, 562, 662, 801, 889
BstNSI RCATGY 2 cut(s) 551, 669
BstSCI CCNGG 2 cut(s) 702, 823
BstSLI GKGCMC 1 cut(s) 658
BstSNI TACGTA 1 cut(s) 687
BstUI CGCG 2 cut(s) 304, 510
BstV1I GCAGC 1 cut(s) 508
BstX2I RGATCY 1 cut(s) 315
BstXI CCANNNNNNTGG 2 cut(s) 194, 726
BstYI RGATCY 1 cut(s) 315
BstZI CGGCCG 1 cut(s) 733
Bsu36I CCTNAGG 1 cut(s) 1003
BsuI GTATCC 1 cut(s) 1094
BsuRI GGCC 4 cut(s) 186, 735, 844, 902
BtgI CCRYGG 1 cut(s) 187
BtrI CACGTC 1 cut(s) 479
BtsCI GGATG 2 cut(s) 199, 1107
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 6 cut(s) 69, 107, 201, 376, 455, 1127
BveI ACCTGC 1 cut(s) 795
Cac8I GCNNGC 1 cut(s) 474
CaiI CAGNNNCTG 2 cut(s) 68, 883
CciI TCATGA 1 cut(s) 381
CciNI GCGGCCGC 1 cut(s) 733
CfoI GCGC 2 cut(s) 304, 540
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 1 cut(s) 821
Csp6I GTAC 5 cut(s) 215, 439, 467, 684, 688
CspAI ACCGGT 1 cut(s) 281
CviQI GTAC 5 cut(s) 215, 439, 467, 684, 688
DdeI CTNAG 2 cut(s) 38, 1003
DpnI GATC 6 cut(s) 162, 317, 393, 717, 876, 1081
DpnII GATC 6 cut(s) 160, 315, 391, 715, 874, 1079
EaeI YGGCCR 3 cut(s) 184, 733, 842
EagI CGGCCG 1 cut(s) 733
Eam1104I CTCTTC 1 cut(s) 950
EarI CTCTTC 1 cut(s) 950
EciI GGCGGA 1 cut(s) 326
EclXI CGGCCG 1 cut(s) 733
Eco105I TACGTA 1 cut(s) 687
Eco130I CCWWGG 2 cut(s) 529, 1018
Eco24I GRGCYC 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 821
Eco52I CGGCCG 1 cut(s) 733
Eco81I CCTNAGG 1 cut(s) 1003
Eco88I CYCGRG 2 cut(s) 164, 491
EcoT14I CCWWGG 2 cut(s) 529, 1018
EcoT22I ATGCAT 1 cut(s) 239
EcoT38I GRGCYC 1 cut(s) 704
ErhI CCWWGG 2 cut(s) 529, 1018
FaqI GGGAC 3 cut(s) 97, 584, 1102
FauI CCCGC 1 cut(s) 643
Fnu4HI GCNGC 5 cut(s) 199, 276, 497, 733, 736
FokI GGATG 2 cut(s) 206, 1094
FriOI GRGCYC 1 cut(s) 704
Fsp4HI GCNGC 5 cut(s) 199, 276, 497, 733, 736
GlaI GCGC 2 cut(s) 303, 539
GluI GCNGC 5 cut(s) 199, 276, 497, 733, 736
GsaI CCCAGC 1 cut(s) 119
GsuI CTGGAG 4 cut(s) 106, 120, 777, 905
HaeIII GGCC 4 cut(s) 186, 735, 844, 902
HapII CCGG 8 cut(s) 91, 104, 282, 389, 704, 824, 893, 916
HhaI GCGC 2 cut(s) 304, 540
Hin6I GCGC 2 cut(s) 302, 538
HinP1I GCGC 2 cut(s) 302, 538
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 3 cut(s) 131, 332, 385
HpaII CCGG 8 cut(s) 91, 104, 282, 389, 704, 824, 893, 916
HphI GGTGA 3 cut(s) 76, 327, 1079
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 3 cut(s) 160, 483, 879
Hpy8I GTNNAC 1 cut(s) 331
Hpy99I CGWCG 1 cut(s) 850
HpyAV CCTTC 4 cut(s) 15, 440, 591, 937
HpyCH4III ACNGT 4 cut(s) 111, 205, 371, 602
HpyCH4IV ACGT 6 cut(s) 217, 342, 478, 686, 798, 848
HpyF10VI GCNNNNNNNGC 5 cut(s) 308, 562, 662, 801, 889
HpyF3I CTNAG 2 cut(s) 38, 1003
HpySE526I ACGT 6 cut(s) 217, 342, 478, 686, 798, 848
HspAI GCGC 2 cut(s) 302, 538
Kpn2I TCCGGA 2 cut(s) 388, 915
Kzo9I GATC 6 cut(s) 160, 315, 391, 715, 874, 1079
LmnI GCTCC 1 cut(s) 93
Lsp1109I GCAGC 1 cut(s) 508
LweI GCATC 3 cut(s) 34, 879, 1116
MaeII ACGT 6 cut(s) 217, 342, 478, 686, 798, 848
MaeIII GTNAC 6 cut(s) 64, 218, 638, 668, 768, 1067
MalI GATC 6 cut(s) 162, 317, 393, 717, 876, 1081
MboI GATC 6 cut(s) 160, 315, 391, 715, 874, 1079
MboII GAAGA 1 cut(s) 967
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 3 cut(s) 478, 658, 704
MlsI TGGCCA 1 cut(s) 186
MluCI AATT 1 cut(s) 816
MluNI TGGCCA 1 cut(s) 186
MlyI GAGTC 3 cut(s) 125, 326, 394
Mox20I TGGCCA 1 cut(s) 186
Mph1103I ATGCAT 1 cut(s) 239
MroI TCCGGA 2 cut(s) 388, 915
MscI TGGCCA 1 cut(s) 186
MslI CAYNNNNRTG 1 cut(s) 192
Msp20I TGGCCA 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 8 cut(s) 91, 104, 282, 389, 704, 824, 893, 916
MspR9I CCNGG 2 cut(s) 704, 825
MvnI CGCG 2 cut(s) 304, 510
MwoI GCNNNNNNNGC 5 cut(s) 308, 562, 662, 801, 889
NciI CCSGG 2 cut(s) 704, 825
NdeII GATC 6 cut(s) 160, 315, 391, 715, 874, 1079
NlaIV GGNNCC 1 cut(s) 655
NmuCI GTSAC 6 cut(s) 64, 218, 638, 668, 768, 1067
NotI GCGGCCGC 1 cut(s) 733
NsiI ATGCAT 1 cut(s) 239
NspI RCATGY 2 cut(s) 551, 669
PaeR7I CTCGAG 1 cut(s) 164
PagI TCATGA 1 cut(s) 381
Pfl23II CGTACG 1 cut(s) 687
PinAI ACCGGT 1 cut(s) 281
PkrI GCNGC 5 cut(s) 200, 277, 498, 734, 737
PleI GAGTC 3 cut(s) 125, 326, 393
PpsI GAGTC 3 cut(s) 125, 326, 393
Ppu21I YACGTR 1 cut(s) 687
PsiI TTATAA 1 cut(s) 347
PspFI CCCAGC 1 cut(s) 115
PspLI CGTACG 1 cut(s) 687
PspN4I GGNNCC 1 cut(s) 655
PspPI GGNCC 1 cut(s) 821
PstNI CAGNNNCTG 2 cut(s) 68, 883
PsuI RGATCY 1 cut(s) 315
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 5 cut(s) 216, 440, 468, 685, 689
RsaNI GTAC 5 cut(s) 215, 439, 467, 684, 688
RseI CAYNNNNRTG 1 cut(s) 192
SatI GCNGC 5 cut(s) 199, 276, 497, 733, 736
Sau3AI GATC 6 cut(s) 160, 315, 391, 715, 874, 1079
Sau96I GGNCC 1 cut(s) 821
SchI GAGTC 3 cut(s) 125, 326, 394
ScrFI CCNGG 2 cut(s) 704, 825
SduI GDGCHC 3 cut(s) 478, 658, 704
SfaNI GCATC 3 cut(s) 34, 879, 1116
Sfr274I CTCGAG 1 cut(s) 164
SinI GGWCC 1 cut(s) 821
SlaI CTCGAG 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 192
SmlI CTYRAG 2 cut(s) 164, 938
SmoI CTYRAG 2 cut(s) 164, 938
SnaBI TACGTA 1 cut(s) 687
Sse9I AATT 1 cut(s) 816
StyD4I CCNGG 2 cut(s) 702, 823
StyI CCWWGG 2 cut(s) 529, 1018
TaaI ACNGT 4 cut(s) 111, 205, 371, 602
TaiI ACGT 6 cut(s) 220, 345, 481, 689, 801, 851
TaqI TCGA 3 cut(s) 165, 264, 998
TasI AATT 1 cut(s) 816
TatI WGTACW 2 cut(s) 438, 466
TauI GCSGC 4 cut(s) 201, 278, 735, 738
TscAI CASTG 6 cut(s) 69, 114, 208, 376, 462, 1127
TseFI GTSAC 6 cut(s) 64, 218, 638, 668, 768, 1067
TseI GCWGC 1 cut(s) 496
Tsp45I GTSAC 6 cut(s) 64, 218, 638, 668, 768, 1067
TspDTI ATGAA 3 cut(s) 370, 913, 1026
TspGWI ACGGA 1 cut(s) 1100
TspRI CASTG 6 cut(s) 69, 114, 208, 376, 462, 1127
VpaK11BI GGWCC 1 cut(s) 821
XceI RCATGY 2 cut(s) 551, 669
XhoI CTCGAG 1 cut(s) 164
Zsp2I ATGCAT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.