RLG00000005197

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017181
Physical Location & Seq
Reverse (-)
65830379 .. 65832310
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005197

Sequence Viewer

Length: 1713 bp
ATGAACACCTGTTCACACCTTTGCTCTTCTTTGTTAAACAAATGCAAGACCCTCAAAACCGTGAAGCAAGTCCACGCCATTGTCCACAAGACCGGGCTCGACACCGACCCATTGGTATTCGGCAAGCTCCTCCTCCACTGCGCCGTCACAATCTCCGACGCACTCGACTACGCTCGCCGCCTCTTTCTTCATTTTCCATACCCAGATGCGTTCATGTACAACACCCTTATCCGCGGACTCGCCGACTCCGATAACCCACACAATGCACTTCTCCTGTTCCATCACATGCGTCGGAAATATGTAAGACCGATCGATAGCTTCTCTTTCGCTTTCACACTTAAGGCGGCGGCGAATTGCGGGTCGCTGAGGGAAGGGATGCAACTGCATTGTCAGGGTTTGTTGCATGGGCTACATACTCATGTTTTCGTTGGGACAACTCTGGTTAGTATGTATGGTGAATGTGGGTGTATAGGTTTTGCACGGAAGGTGTTCGATGAAATGCCTGAACCGAATGTGGTCGCGTGGAACGCGGTGCTTACGGCGTGTTTTAAGTGTGGGGATGTGGAGGGTGCGGAGGAAGTGTTTGGATCAATGCCTTGGAAAGATTTGACTTCGTGGAATATTGTGGTTGCGGGATACGTGAAGGCGGATGAGCTTGAGCTGGCGAAGGAGGCGTTTTGGAGGATGCCGGTGAAGGATGATGTGTCTTGGAGTACTATGATTGTTGGGTTTGCGCAGAGCGGTTGTTTTGATGAGGCTTTTGGGTTTTTCGGGGAGTTACAGCGGAAGGGTGTTAGGCCAAATGAGGCTAGCTTGAGTGGGGTGCTGTCTGCATGTGCGCAGGCTGGAGCGGTTGAGTTTGGTAGAATTTTTCATGGTTTGGTGGAGAAGGCTGGATTCCTTTCGATGATTTCAGTCAATAATGCACTCCTGGATATGTACTCTAAGTCTGGAAATGTGGAGATGGCTCGGTTGGTCTTTGAAAGAATGCCGGAGAGAAAGAGTGTTGTTTCTTGGACTTCGATGATAGCAGCACTTGCAATGCATGGCTATGGCAAAGAAGCAATACAAGTTTTCCGGAGGATGGAAGCGTCTGGAGTTAGGCCTGATGGGATCGCATTCATTTCTGTTTTGTATGCTTGTAGTCATGCTGGGTTGGTTAACGAAGGATGTGAATATTTCTCTAAGATGAGAGATTTGTATGGTATAGAACCAGCTATTGACCATTACGGTTGTATGGTAGACCTGTATGGTCGAGCTGGAAAACTGCAGAAAGCCTATGATTTTAAGATTAAGAAAACTCCAGGTTGGAGCGTGATTGAAGTTGACAAGGTCATGTATAGCTTTGTGGCAGGCGATAAATCAAACAAGATCACAGAAGAGGCTTATGAGAAATTAAGGGAGATAATGCTGAAACTTCGTGTTGGTGGAGGTTATTTGCCGGAGGTTGGGAGTGTTCTGCATGACATAGAAGCGGAGGACAAAGAAGATTCAGTGTTCATGCACAGTGAGAAACTTGCAGTAGCTTTTGGGTTGGCAAGATTGCCTGAGGGAAGGATGATAAGGATAGTGAAGAATTTGCGGGTTTGTAGAGACTGTCATACAGTGATGAAGATGATTTCTAAGGTCTATAGGTCGGAGATAGTGGTGAGGGATAGAAGCCGCTTTCACTCCTTCAAAGATGGCTCTTGTTCATGCAACGATTACTGGTGA

Protein Analysis

571

Amino Acids

63.77

Weight (kDa)

7.8

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 170 - 199 1e-05 PPR repeat family
PPR_2 PF13041 233 - 280 1.6e-08 PPR repeat family
PPR PF01535 235 - 265 5e-07 PPR repeat
PPR_2 PF13041 335 - 382 8.3e-08 PPR repeat family
PPR PF01535 337 - 367 6.6e-07 PPR repeat
DYW_deaminase PF14432 478 - 570 7.4e-39 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017181)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74630 AT1G74630
fragaria_vesca FvH4_5g14980
malus_domestica MD14G1199700.v1.1
prunus_persica Prupe.5G193000_v2.0.a1
pyrus_communis pycom14g16550
rosa_chinensis RchiOBHm_Chr7g0181341
rosa_laevigata RLG00000005197
rosa_rugosa Rorug06G0440400
rosa_samantha Rh7AG042300 Rh7BG041200 Rh7CG043600
rosa_wichuraiana Rw7G003490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 735, 840
AccBSI CCGCTC 2 cut(s) 741, 851
AccI GTMKAC 1 cut(s) 1242
AccII CGCG 3 cut(s) 234, 521, 530
AccIII TCCGGA 1 cut(s) 1077
AclWI GGATC 2 cut(s) 595, 1121
AcsI RAATTY 2 cut(s) 867, 1576
AfaI GTAC 3 cut(s) 218, 715, 941
AfiI CCNNNNNNNGG 2 cut(s) 1084, 1448
AflII CTTAAG 1 cut(s) 338
AgsI TTSAA 3 cut(s) 983, 1322, 1678
AjnI CCWGG 2 cut(s) 930, 1303
AluBI AGCT 9 cut(s) 127, 318, 655, 661, 813, 1217, 1259, 1344, 1526
AluI AGCT 9 cut(s) 127, 318, 655, 661, 813, 1217, 1259, 1344, 1526
Alw26I GTCTC 1 cut(s) 1587
AlwI GGATC 2 cut(s) 595, 1121
Aor13HI TCCGGA 1 cut(s) 1077
AoxI GGCC 2 cut(s) 797, 1103
ApeKI GCWGC 1 cut(s) 1031
ApoI RAATTY 2 cut(s) 867, 1576
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
Asp700I GAANNNNTTC 1 cut(s) 488
AspLEI GCGC 3 cut(s) 143, 736, 841
AsuC2I CCSGG 1 cut(s) 94
AsuHPI GGTGA 3 cut(s) 467, 703, 1660
AsuNHI GCTAGC 1 cut(s) 809
AxyI CCTNAGG 1 cut(s) 1548
BanII GRGCYC 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 365
BbvI GCAGC 1 cut(s) 1043
BccI CCATC 5 cut(s) 288, 958, 1078, 1103, 1676
BceAI ACGGC 2 cut(s) 128, 555
BciT130I CCWGG 2 cut(s) 932, 1305
BciVI GTATCC 1 cut(s) 629
BcnI CCSGG 1 cut(s) 94
BcoDI GTCTC 1 cut(s) 1587
BfaI CTAG 1 cut(s) 810
BfmI CTRYAG 2 cut(s) 1268, 1630
BfrI CTTAAG 1 cut(s) 338
BfuI GTATCC 1 cut(s) 629
BisI GCNGC 5 cut(s) 178, 345, 348, 1032, 1663
BlsI GCNGC 5 cut(s) 179, 346, 349, 1033, 1664
BmcAI AGTACT 1 cut(s) 715
Bme1390I CCNGG 3 cut(s) 94, 932, 1305
BmrFI CCNGG 3 cut(s) 94, 932, 1305
BmsI GCATC 3 cut(s) 196, 366, 675
BmtI GCTAGC 1 cut(s) 813
BpmI CTGGAG 3 cut(s) 867, 1116, 1287
Bpu10I CCTNAGC 1 cut(s) 365
BpuEI CTTGAG 2 cut(s) 677, 835
BpuMI CCSGG 1 cut(s) 94
Bsa29I ATCGAT 1 cut(s) 312
BsaAI YACGTR 1 cut(s) 640
BsaJI CCNNGG 2 cut(s) 232, 596
BsaWI WCCGGW 1 cut(s) 1077
BsaXI ACNNNNNCTCC 2 cut(s) 566, 596
Bsc4I CCNNNNNNNGG 2 cut(s) 1084, 1448
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 1 cut(s) 1712
Bse21I CCTNAGG 1 cut(s) 1548
Bse3DI GCAATG 1 cut(s) 1047
BseAI TCCGGA 1 cut(s) 1077
BseBI CCWGG 2 cut(s) 932, 1305
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 2 cut(s) 232, 596
BseGI GGATG 8 cut(s) 381, 565, 655, 690, 703, 1089, 1175, 1563
BseLI CCNNNNNNNGG 2 cut(s) 1084, 1448
BseMI GCAATG 1 cut(s) 1047
BseMII CTCAG 2 cut(s) 356, 1539
BseNI ACTGG 1 cut(s) 1712
BseRI GAGGAG 2 cut(s) 119, 122
BseXI GCAGC 1 cut(s) 1043
BseYI CCCAGC 1 cut(s) 1151
Bsh1236I CGCG 3 cut(s) 234, 521, 530
Bsh1285I CGRYCG 1 cut(s) 312
BshFI GGCC 2 cut(s) 799, 1105
BshVI ATCGAT 1 cut(s) 312
BsiEI CGRYCG 1 cut(s) 312
BsiSI CCGG 5 cut(s) 93, 689, 992, 1078, 1442
BslFI GGGAC 1 cut(s) 445
BslI CCNNNNNNNGG 2 cut(s) 1084, 1448
BsmAI GTCTC 1 cut(s) 1587
BsmFI GGGAC 1 cut(s) 445
BsmI GAATGC 2 cut(s) 993, 1118
BsnI GGCC 2 cut(s) 799, 1105
Bsp1286I GDGCHC 1 cut(s) 99
Bsp13I TCCGGA 1 cut(s) 1077
Bsp1407I TGTACA 1 cut(s) 216
Bsp143I GATC 4 cut(s) 309, 587, 1113, 1371
BspANI GGCC 2 cut(s) 799, 1105
BspCNI CTCAG 2 cut(s) 357, 1540
BspDI ATCGAT 1 cut(s) 312
BspEI TCCGGA 1 cut(s) 1077
BspFNI CGCG 3 cut(s) 234, 521, 530
BspMAI CTGCAG 1 cut(s) 1272
BspOI GCTAGC 1 cut(s) 813
BspPI GGATC 2 cut(s) 595, 1121
BspQI GCTCTTC 1 cut(s) 31
BspTI CTTAAG 1 cut(s) 338
BsrBI CCGCTC 2 cut(s) 741, 851
BsrDI GCAATG 1 cut(s) 1047
BsrFI RCCGGY 1 cut(s) 688
BsrGI TGTACA 1 cut(s) 216
BsrI ACTGG 1 cut(s) 1712
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 2 cut(s) 232, 596
BssMI GATC 4 cut(s) 309, 587, 1113, 1371
BssT1I CCWWGG 1 cut(s) 596
Bst2UI CCWGG 2 cut(s) 932, 1305
Bst4CI ACNGT 5 cut(s) 61, 1232, 1508, 1598, 1606
Bst6I CTCTTC 2 cut(s) 31, 1374
BstAFI CTTAAG 1 cut(s) 338
BstAPI GCANNNNNTGC 1 cut(s) 1037
BstAUI TGTACA 1 cut(s) 216
BstBAI YACGTR 1 cut(s) 640
BstC8I GCNNGC 6 cut(s) 125, 175, 663, 811, 843, 1354
BstDEI CTNAG 5 cut(s) 365, 945, 1185, 1548, 1623
BstDSI CCRYGG 1 cut(s) 232
BstF5I GGATG 8 cut(s) 381, 565, 655, 690, 703, 1089, 1175, 1563
BstFNI CGCG 3 cut(s) 234, 521, 530
BstHHI GCGC 3 cut(s) 143, 736, 841
BstKTI GATC 4 cut(s) 312, 590, 1116, 1374
BstMAI GTCTC 1 cut(s) 1587
BstMBI GATC 4 cut(s) 309, 587, 1113, 1371
BstMCI CGRYCG 1 cut(s) 312
BstMWI GCNNNNNNNGC 3 cut(s) 527, 671, 1037
BstNI CCWGG 2 cut(s) 932, 1305
BstNSI RCATGY 2 cut(s) 289, 837
BstSCI CCNGG 3 cut(s) 92, 930, 1303
BstSFI CTRYAG 2 cut(s) 1268, 1630
BstUI CGCG 3 cut(s) 234, 521, 530
BstV1I GCAGC 1 cut(s) 1043
Bsu15I ATCGAT 1 cut(s) 312
Bsu36I CCTNAGG 1 cut(s) 1548
BsuI GTATCC 1 cut(s) 629
BsuRI GGCC 2 cut(s) 799, 1105
BsuTUI ATCGAT 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 232
BtsCI GGATG 8 cut(s) 381, 565, 655, 690, 703, 1089, 1175, 1563
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 4 cut(s) 136, 1500, 1513, 1611
Cac8I GCNNGC 6 cut(s) 125, 175, 663, 811, 843, 1354
CfoI GCGC 3 cut(s) 143, 736, 841
Cfr10I RCCGGY 1 cut(s) 688
Cfr42I CCGCGG 1 cut(s) 235
ClaI ATCGAT 1 cut(s) 312
CseI GACGC 3 cut(s) 167, 278, 1080
Csp6I GTAC 3 cut(s) 217, 714, 940
CviQI GTAC 3 cut(s) 217, 714, 940
DdeI CTNAG 5 cut(s) 365, 945, 1185, 1548, 1623
DpnI GATC 4 cut(s) 311, 589, 1115, 1373
DpnII GATC 4 cut(s) 309, 587, 1113, 1371
Eam1104I CTCTTC 2 cut(s) 31, 1374
EarI CTCTTC 2 cut(s) 31, 1374
EciI GGCGGA 1 cut(s) 662
Eco130I CCWWGG 1 cut(s) 596
Eco147I AGGCCT 1 cut(s) 1105
Eco24I GRGCYC 1 cut(s) 99
Eco81I CCTNAGG 1 cut(s) 1548
EcoRII CCWGG 2 cut(s) 930, 1303
EcoT14I CCWWGG 1 cut(s) 596
EcoT22I ATGCAT 1 cut(s) 1047
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 596
FaqI GGGAC 1 cut(s) 445
FauI CCCGC 3 cut(s) 350, 625, 1575
FblI GTMKAC 1 cut(s) 1242
Fnu4HI GCNGC 5 cut(s) 178, 345, 348, 1032, 1663
FokI GGATG 8 cut(s) 388, 572, 662, 697, 710, 1096, 1182, 1570
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 5 cut(s) 178, 345, 348, 1032, 1663
FspBI CTAG 1 cut(s) 810
FspI TGCGCA 2 cut(s) 735, 840
GlaI GCGC 3 cut(s) 142, 735, 840
GluI GCNGC 5 cut(s) 178, 345, 348, 1032, 1663
GsaI CCCAGC 1 cut(s) 1155
GsuI CTGGAG 3 cut(s) 867, 1116, 1287
HaeIII GGCC 2 cut(s) 799, 1105
HapII CCGG 5 cut(s) 93, 689, 992, 1078, 1442
HgaI GACGC 3 cut(s) 167, 278, 1080
HhaI GCGC 3 cut(s) 143, 736, 841
Hin6I GCGC 3 cut(s) 141, 734, 839
HinP1I GCGC 3 cut(s) 141, 734, 839
HincII GTYRAC 2 cut(s) 1162, 1327
HindII GTYRAC 2 cut(s) 1162, 1327
HinfI GANTC 4 cut(s) 237, 245, 897, 1490
HpaI GTTAAC 1 cut(s) 1162
HpaII CCGG 5 cut(s) 93, 689, 992, 1078, 1442
HphI GGTGA 3 cut(s) 467, 703, 1660
Hpy166II GTNNAC 6 cut(s) 14, 73, 85, 1162, 1243, 1327
Hpy188I TCNGA 4 cut(s) 157, 250, 294, 1639
Hpy188III TCNNGA 3 cut(s) 951, 1078, 1095
Hpy8I GTNNAC 6 cut(s) 14, 73, 85, 1162, 1243, 1327
Hpy99I CGWCG 2 cut(s) 161, 294
HpyCH4III ACNGT 5 cut(s) 61, 1232, 1508, 1598, 1606
HpyCH4IV ACGT 1 cut(s) 639
HpyF10VI GCNNNNNNNGC 3 cut(s) 527, 671, 1037
HpyF3I CTNAG 5 cut(s) 365, 945, 1185, 1548, 1623
HpySE526I ACGT 1 cut(s) 639
HspAI GCGC 3 cut(s) 141, 734, 839
Kpn2I TCCGGA 1 cut(s) 1077
KspAI GTTAAC 1 cut(s) 1162
KspI CCGCGG 1 cut(s) 235
Kzo9I GATC 4 cut(s) 309, 587, 1113, 1371
LguI GCTCTTC 1 cut(s) 31
LmnI GCTCC 3 cut(s) 132, 848, 1311
Lsp1109I GCAGC 1 cut(s) 1043
LweI GCATC 3 cut(s) 196, 366, 675
MaeI CTAG 1 cut(s) 810
MaeII ACGT 1 cut(s) 639
MaeIII GTNAC 2 cut(s) 145, 777
MalI GATC 4 cut(s) 311, 589, 1115, 1373
MbiI CCGCTC 2 cut(s) 741, 851
MboI GATC 4 cut(s) 309, 587, 1113, 1371
MboII GAAGA 6 cut(s) 18, 179, 1391, 1499, 1585, 1624
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 4 cut(s) 352, 867, 1394, 1576
MlyI GAGTC 2 cut(s) 231, 239
MmeI TCCRAC 4 cut(s) 180, 272, 1289, 1617
Mph1103I ATGCAT 1 cut(s) 1047
MroI TCCGGA 1 cut(s) 1077
MroXI GAANNNNTTC 1 cut(s) 488
MseI TTAA 7 cut(s) 35, 339, 549, 1161, 1287, 1293, 1397
MslI CAYNNNNRTG 2 cut(s) 417, 1050
MspA1I CMGCKG 2 cut(s) 234, 784
MspCI CTTAAG 1 cut(s) 338
MspI CCGG 5 cut(s) 93, 689, 992, 1078, 1442
MspR9I CCNGG 3 cut(s) 94, 932, 1305
Mva1269I GAATGC 2 cut(s) 993, 1118
MvaI CCWGG 2 cut(s) 932, 1305
MvnI CGCG 3 cut(s) 234, 521, 530
MwoI GCNNNNNNNGC 3 cut(s) 527, 671, 1037
NciI CCSGG 1 cut(s) 94
NdeII GATC 4 cut(s) 309, 587, 1113, 1371
NheI GCTAGC 1 cut(s) 809
NmuCI GTSAC 1 cut(s) 145
NsbI TGCGCA 2 cut(s) 735, 840
NsiI ATGCAT 1 cut(s) 1047
NspI RCATGY 2 cut(s) 289, 837
PceI AGGCCT 1 cut(s) 1105
PciSI GCTCTTC 1 cut(s) 31
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PctI GAATGC 2 cut(s) 993, 1118
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 2 cut(s) 897, 1490
PflFI GACNNNGTC 1 cut(s) 1331
PfoI TCCNGGA 1 cut(s) 930
PkrI GCNGC 5 cut(s) 179, 346, 349, 1033, 1664
Ple19I CGATCG 1 cut(s) 312
PleI GAGTC 2 cut(s) 231, 239
PpsI GAGTC 2 cut(s) 231, 239
Ppu21I YACGTR 1 cut(s) 640
Psp6I CCWGG 2 cut(s) 930, 1303
PspFI CCCAGC 1 cut(s) 1151
PspGI CCWGG 2 cut(s) 930, 1303
PstI CTGCAG 1 cut(s) 1272
PsyI GACNNNGTC 1 cut(s) 1331
PvuI CGATCG 1 cut(s) 312
RsaI GTAC 3 cut(s) 218, 715, 941
RsaNI GTAC 3 cut(s) 217, 714, 940
RseI CAYNNNNRTG 2 cut(s) 417, 1050
SacII CCGCGG 1 cut(s) 235
SapI GCTCTTC 1 cut(s) 31
SaqAI TTAA 7 cut(s) 35, 339, 549, 1161, 1287, 1293, 1397
SatI GCNGC 5 cut(s) 178, 345, 348, 1032, 1663
Sau3AI GATC 4 cut(s) 309, 587, 1113, 1371
ScaI AGTACT 1 cut(s) 715
SchI GAGTC 2 cut(s) 231, 239
ScrFI CCNGG 3 cut(s) 94, 932, 1305
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 3 cut(s) 196, 366, 675
SfcI CTRYAG 2 cut(s) 1268, 1630
Sfr303I CCGCGG 1 cut(s) 235
SgrBI CCGCGG 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 417, 1050
SmlI CTYRAG 3 cut(s) 338, 656, 814
SmoI CTYRAG 3 cut(s) 338, 656, 814
Sse9I AATT 4 cut(s) 352, 867, 1394, 1576
SseBI AGGCCT 1 cut(s) 1105
SspI AATATT 2 cut(s) 622, 1178
SspMI CTAG 1 cut(s) 810
StuI AGGCCT 1 cut(s) 1105
StyD4I CCNGG 3 cut(s) 92, 930, 1303
StyI CCWWGG 1 cut(s) 596
TaaI ACNGT 5 cut(s) 61, 1232, 1508, 1598, 1606
TaiI ACGT 1 cut(s) 642
TaqI TCGA 7 cut(s) 99, 165, 312, 492, 905, 1022, 1255
TaqII GACCGA 1 cut(s) 322
TasI AATT 4 cut(s) 352, 867, 1394, 1576
TatI WGTACW 3 cut(s) 216, 713, 939
TauI GCSGC 4 cut(s) 180, 347, 350, 1665
TfiI GAWTC 2 cut(s) 897, 1490
Tru1I TTAA 7 cut(s) 35, 339, 549, 1161, 1287, 1293, 1397
Tru9I TTAA 7 cut(s) 35, 339, 549, 1161, 1287, 1293, 1397
TscAI CASTG 4 cut(s) 143, 1500, 1513, 1611
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 1 cut(s) 1031
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 9 cut(s) 17, 179, 202, 510, 863, 1111, 1489, 1625, 1683
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 4 cut(s) 143, 1500, 1513, 1611
Tth111I GACNNNGTC 1 cut(s) 1331
Vha464I CTTAAG 1 cut(s) 338
XapI RAATTY 2 cut(s) 867, 1576
XceI RCATGY 2 cut(s) 289, 837
XmiI GTMKAC 1 cut(s) 1242
XmnI GAANNNNTTC 1 cut(s) 488
XspI CTAG 1 cut(s) 810
ZrmI AGTACT 1 cut(s) 715
Zsp2I ATGCAT 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.