Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0018030

Physical Location & Seq

Genomic Coordinates Forward (+)

4350438 .. 4351885

Gene Structure 1448 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000006099

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGGAGTCGACGTCGACACCCAAGCTTCTTCTGAAGCTCACCATCTTCATCTTCATCTTCATCTCTGAAGCTTTGCCCACCAAGTCAGGCTACCTCACAGTCAACTCCACCACAGGCTCTTCTATTTTCTACACCAATTATGAAGCTCAGAGCCTCACTTTCCCTGACCTCTCGCAAACCCCACTTATAATTTGGCTCCAGGGTGGGCCCGGATGCTCCTTCTTGTTTGGTAACTTCATGGAGATCGGGCCTTGGCATGTAAACTTAAACCATCATCATCGTGATCTTGATCATCAACCCTTTGTGCTTGAACCCAACCCCGGTTCTTGGAACCGGATTTTTGGACTTCTTTTCCTTGATAATCCAATAGGATCCGGGTTCAGCATCGCCTCGAGCAGCGAAGAAATCCCAAGAGACCAAATTTCGGTAGCTAAACACCTATACATTGCGATTATAAAGTTTATTGAACTTGATCCAATATTGTTTAGCTCTCGTCCGCTATATATAGCGGGCGAGAGCTATGCTGGCAAGTACGTGCCAGCAATAGGGTACTATATACTCAAGAAGAATGAAGAGTCCAATCATTCGGTTAATTTAGCTGGCGTAGCTATAGGAAATGGTTTGATAGACCCGGAGACTCAGGTGGGCACTCACGCAGACAATGCTTACTTTTCGGGCTTGATCAATGAGAAACAGAGGAGGGAGATGGAGATGTATCAGAACGAGGCAATCAGGCTAGCCAGATTAAAGCGATGGAGAGATGCAACCGATGCAAGGTACCGAGTTGTGGATATGCTGATTGACATGACAGGATTAGCTACATTGTTTGACTACTCCAAGAAAGCGCCTTACCACAAGACTCAATGGGTCACTGACTTTTTACATGATGAAAGGGTCAAGAGAATTATGAGTGTGGAGGAGTCCGCAGTGTTTAGAAATTGTAGCCATGTGGTGCAAGTTGCTTTGTATGATGATAACATGAAGCGTGTGAAGTACATGGTGGACTTGTTGGTGAAGAGGAGCAAGACATTGCAATGGGAGGAGATTCAGAGATTTTTAGCGGCGGGGAGGAGGGTTTGGAGAGGGAGAGGGGATGAGCTTGCAGGGTATGTGCAGAAATGGAAGAGTTTGAGCAATGTTGTGGTTTCAAGAGCAGGGCATCTTGTGCCGGCTGACCAGCCATTGAATTCTCAGGCAATGATCGAAGGCTGGATTTTGGAAACAGGGTTGTTTAGCAACGATGAGCAATTGTTCCGAGTTCTAGAAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004180 GO:0004185 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0017171 GO:0019538 GO:0030163 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0070008 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S10

Protein Analysis

424

Amino Acids

48.21

Weight (kDa)

6.47

Isoelectric Point (pI)

41.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S10	PF00450	26 - 340	9.2e-70	Serine carboxypeptidase
Peptidase_S10	PF00450	342 - 405	4.8e-08	Serine carboxypeptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0018030)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g32490
rosa_chinensis	RchiOBHm_Chr4g0441351
rosa_laevigata	RLG00000006099
rosa_multiflora	Rmu_co8458681.1_g000001 Rmu_sc0005516.1_g000007
rosa_roxburghii	Rroxscaffold_5G00382240
rosa_rugosa	Rorug04G0332500
rosa_samantha	Rh4AG384600 Rh4BG398700 Rh4CG413800
rosa_wichuraiana	Rw4G033200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	188, 455
AatII	GACGTC	1 cut(s)	14
Acc65I	GGTACC	1 cut(s)	777
AccB1I	GGYRCC	1 cut(s)	777
AccI	GTMKAC	2 cut(s)	8, 14
AciI	CCGC	5 cut(s)	497, 509, 924, 1061, 1064
AclWI	GGATC	3 cut(s)	366, 379, 467
AcsI	RAATTY	2 cut(s)	420, 1186
AcuI	CTGAAG	2 cut(s)	53, 87
AcyI	GRCGYC	1 cut(s)	11
AfaI	GTAC	4 cut(s)	533, 551, 779, 995
AfiI	CCNNNNNNNGG	6 cut(s)	320, 327, 424, 545, 774, 787
AgsI	TTSAA	4 cut(s)	311, 467, 1149, 1186
AjnI	CCWGG	1 cut(s)	198
Alw26I	GTCTC	2 cut(s)	408, 629
AlwI	GGATC	3 cut(s)	366, 379, 467
Ama87I	CYCGRG	1 cut(s)	391
AoxI	GGCC	2 cut(s)	206, 248
ApaI	GGGCCC	1 cut(s)	210
ApeKI	GCWGC	1 cut(s)	396
ApoI	RAATTY	2 cut(s)	420, 1186
Asp718I	GGTACC	1 cut(s)	777
AspLEI	GCGC	1 cut(s)	847
AspS9I	GGNCC	3 cut(s)	206, 207, 248
AsuC2I	CCSGG	4 cut(s)	210, 321, 376, 632
AsuHPI	GGTGA	2 cut(s)	31, 1024
AsuNHI	GCTAGC	1 cut(s)	736
AvaI	CYCGRG	1 cut(s)	391
BaeGI	GKGCMC	2 cut(s)	210, 650
BamHI	GGATCC	1 cut(s)	371
BanI	GGYRCC	1 cut(s)	777
BanII	GRGCYC	1 cut(s)	210
BbvI	GCAGC	1 cut(s)	408
BccI	CCATC	4 cut(s)	50, 279, 700, 747
BcgI	CGANNNNNNTGC	2 cut(s)	503, 537
BciT130I	CCWGG	1 cut(s)	200
BclI	TGATCA	2 cut(s)	289, 681
BcnI	CCSGG	4 cut(s)	210, 321, 376, 632
BcoDI	GTCTC	2 cut(s)	408, 629
BfaI	CTAG	2 cut(s)	737, 1262
BfmI	CTRYAG	1 cut(s)	609
BfoI	RGCGCY	1 cut(s)	848
BisI	GCNGC	2 cut(s)	397, 1062
BlsI	GCNGC	2 cut(s)	398, 1063
Bme1390I	CCNGG	5 cut(s)	200, 210, 321, 376, 632
BmeT110I	CYCGRG	1 cut(s)	391
BmgT120I	GGNCC	3 cut(s)	206, 207, 248
BmiI	GGNNCC	5 cut(s)	197, 208, 332, 373, 779
BmrFI	CCNGG	5 cut(s)	200, 210, 321, 376, 632
BmsI	GCATC	5 cut(s)	203, 393, 751, 760, 1168
BmtI	GCTAGC	1 cut(s)	740
BpmI	CTGGAG	1 cut(s)	182
BpuEI	CTTGAG	1 cut(s)	545
BpuMI	CCSGG	4 cut(s)	210, 321, 376, 632
BsaAI	YACGTR	1 cut(s)	535
BsaBI	GATNNNNATC	1 cut(s)	288
BsaHI	GRCGYC	1 cut(s)	11
BsaI	GGTCTC	1 cut(s)	408
BsaJI	CCNNGG	3 cut(s)	199, 251, 319
BsaWI	WCCGGW	1 cut(s)	333
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	6 cut(s)	320, 327, 424, 545, 774, 787
Bse118I	RCCGGY	1 cut(s)	1168
Bse3DI	GCAATG	5 cut(s)	444, 1028, 1040, 1141, 1203
Bse8I	GATNNNNATC	1 cut(s)	288
BseBI	CCWGG	1 cut(s)	200
BseDI	CCNNGG	3 cut(s)	199, 251, 319
BseGI	GGATG	2 cut(s)	218, 1099
BseJI	GATNNNNATC	1 cut(s)	288
BseLI	CCNNNNNNNGG	6 cut(s)	320, 327, 424, 545, 774, 787
BseMI	GCAATG	5 cut(s)	444, 1028, 1040, 1141, 1203
BseMII	CTCAG	3 cut(s)	161, 653, 1205
BseRI	GAGGAG	5 cut(s)	712, 932, 1033, 1055, 1084
BseSI	GKGCMC	2 cut(s)	210, 650
BseXI	GCAGC	1 cut(s)	408
BsgI	GTGCAG	1 cut(s)	1133
BshFI	GGCC	2 cut(s)	208, 250
BshNI	GGYRCC	1 cut(s)	777
BsiHKCI	CYCGRG	1 cut(s)	391
BsiSI	CCGG	6 cut(s)	210, 321, 334, 375, 632, 1169
BslI	CCNNNNNNNGG	6 cut(s)	320, 327, 424, 545, 774, 787
BsmAI	GTCTC	2 cut(s)	408, 629
BsnI	GGCC	2 cut(s)	208, 250
Bso31I	GGTCTC	1 cut(s)	408
BsoBI	CYCGRG	1 cut(s)	391
Bsp120I	GGGCCC	1 cut(s)	206
Bsp1286I	GDGCHC	2 cut(s)	210, 650
Bsp143I	GATC	7 cut(s)	243, 283, 289, 371, 472, 681, 1200
BspACI	CCGC	5 cut(s)	497, 509, 924, 1061, 1064
BspANI	GGCC	2 cut(s)	208, 250
BspCNI	CTCAG	3 cut(s)	160, 652, 1204
BspLI	GGNNCC	5 cut(s)	197, 208, 332, 373, 779
BspOI	GCTAGC	1 cut(s)	740
BspPI	GGATC	3 cut(s)	366, 379, 467
BspQI	GCTCTTC	1 cut(s)	124
BspT107I	GGYRCC	1 cut(s)	777
BspTNI	GGTCTC	1 cut(s)	408
BsrDI	GCAATG	5 cut(s)	444, 1028, 1040, 1141, 1203
BsrFI	RCCGGY	1 cut(s)	1168
BssAI	RCCGGY	1 cut(s)	1168
BssECI	CCNNGG	3 cut(s)	199, 251, 319
BssMI	GATC	7 cut(s)	243, 283, 289, 371, 472, 681, 1200
BssNI	GRCGYC	1 cut(s)	11
BssT1I	CCWWGG	1 cut(s)	251
Bst2UI	CCWGG	1 cut(s)	200
Bst4CI	ACNGT	1 cut(s)	100
Bst6I	CTCTTC	4 cut(s)	124, 567, 1010, 1118
BstACI	GRCGYC	1 cut(s)	11
BstAPI	GCANNNNNTGC	3 cut(s)	662, 770, 1165
BstBAI	YACGTR	1 cut(s)	535
BstC8I	GCNNGC	7 cut(s)	511, 526, 540, 601, 738, 1101, 1170
BstDEI	CTNAG	3 cut(s)	147, 639, 1191
BstF5I	GGATG	2 cut(s)	218, 1099
BstH2I	RGCGCY	1 cut(s)	848
BstHHI	GCGC	1 cut(s)	847
BstKTI	GATC	7 cut(s)	246, 286, 292, 374, 475, 684, 1203
BstMAI	GTCTC	2 cut(s)	408, 629
BstMBI	GATC	7 cut(s)	243, 283, 289, 371, 472, 681, 1200
BstMWI	GCNNNNNNNGC	5 cut(s)	525, 605, 662, 770, 1165
BstNI	CCWGG	1 cut(s)	200
BstNSI	RCATGY	1 cut(s)	260
BstSCI	CCNGG	5 cut(s)	198, 208, 319, 374, 630
BstSFI	CTRYAG	1 cut(s)	609
BstSLI	GKGCMC	2 cut(s)	210, 650
BstV1I	GCAGC	1 cut(s)	408
BstX2I	RGATCY	1 cut(s)	371
BstYI	RGATCY	1 cut(s)	371
BsuRI	GGCC	2 cut(s)	208, 250
BtgZI	GCGATG	2 cut(s)	370, 766
BtsCI	GGATG	2 cut(s)	218, 1099
BtsI	GCAGTG	1 cut(s)	933
BtsIMutI	CAGTG	2 cut(s)	870, 933
Cac8I	GCNNGC	7 cut(s)	511, 526, 540, 601, 738, 1101, 1170
CfoI	GCGC	1 cut(s)	847
Cfr10I	RCCGGY	1 cut(s)	1168
Cfr13I	GGNCC	3 cut(s)	206, 207, 248
Csp6I	GTAC	4 cut(s)	532, 550, 778, 994
CviAII	CATG	7 cut(s)	238, 257, 805, 884, 947, 979, 997
CviQI	GTAC	4 cut(s)	532, 550, 778, 994
DdeI	CTNAG	3 cut(s)	147, 639, 1191
DpnI	GATC	7 cut(s)	245, 285, 291, 373, 474, 683, 1202
DpnII	GATC	7 cut(s)	243, 283, 289, 371, 472, 681, 1200
Eam1104I	CTCTTC	4 cut(s)	124, 567, 1010, 1118
EarI	CTCTTC	4 cut(s)	124, 567, 1010, 1118
Eco130I	CCWWGG	1 cut(s)	251
Eco24I	GRGCYC	1 cut(s)	210
Eco31I	GGTCTC	1 cut(s)	408
Eco57I	CTGAAG	2 cut(s)	53, 87
Eco88I	CYCGRG	1 cut(s)	391
EcoRI	GAATTC	1 cut(s)	1186
EcoRII	CCWGG	1 cut(s)	198
EcoT14I	CCWWGG	1 cut(s)	251
EcoT38I	GRGCYC	1 cut(s)	210
ErhI	CCWWGG	1 cut(s)	251
FaeI	CATG	7 cut(s)	241, 260, 808, 887, 950, 982, 1000
FatI	CATG	7 cut(s)	237, 256, 804, 883, 946, 978, 996
FauI	CCCGC	2 cut(s)	502, 1057
FbaI	TGATCA	2 cut(s)	289, 681
FblI	GTMKAC	2 cut(s)	8, 14
Fnu4HI	GCNGC	2 cut(s)	397, 1062
FokI	GGATG	2 cut(s)	225, 1106
FriOI	GRGCYC	1 cut(s)	210
Fsp4HI	GCNGC	2 cut(s)	397, 1062
FspBI	CTAG	2 cut(s)	737, 1262
GlaI	GCGC	1 cut(s)	846
GluI	GCNGC	2 cut(s)	397, 1062
GsuI	CTGGAG	1 cut(s)	182
HaeII	RGCGCY	1 cut(s)	848
HaeIII	GGCC	2 cut(s)	208, 250
HapII	CCGG	6 cut(s)	210, 321, 334, 375, 632, 1169
HhaI	GCGC	1 cut(s)	847
Hin1I	GRCGYC	1 cut(s)	11
Hin1II	CATG	7 cut(s)	241, 260, 808, 887, 950, 982, 1000
Hin6I	GCGC	1 cut(s)	845
HinP1I	GCGC	1 cut(s)	845
HincII	GTYRAC	3 cut(s)	9, 15, 103
HindII	GTYRAC	3 cut(s)	9, 15, 103
HindIII	AAGCTT	2 cut(s)	23, 69
HinfI	GANTC	6 cut(s)	5, 575, 637, 859, 920, 1045
HpaII	CCGG	6 cut(s)	210, 321, 334, 375, 632, 1169
HphI	GGTGA	2 cut(s)	31, 1024
Hpy166II	GTNNAC	5 cut(s)	9, 15, 103, 262, 1003
Hpy188I	TCNGA	6 cut(s)	33, 67, 150, 720, 1050, 1256
Hpy188III	TCNNGA	6 cut(s)	281, 287, 562, 898, 1149, 1262
Hpy8I	GTNNAC	5 cut(s)	9, 15, 103, 262, 1003
Hpy99I	CGWCG	2 cut(s)	13, 16
HpyAV	CCTTC	2 cut(s)	229, 1199
HpyCH4III	ACNGT	1 cut(s)	100
HpyCH4IV	ACGT	2 cut(s)	11, 534
HpyCH4V	TGCA	6 cut(s)	764, 773, 955, 1033, 1103, 1114
HpyF10VI	GCNNNNNNNGC	5 cut(s)	525, 605, 662, 770, 1165
HpyF3I	CTNAG	3 cut(s)	147, 639, 1191
HpySE526I	ACGT	2 cut(s)	11, 534
Hsp92I	GRCGYC	1 cut(s)	11
Hsp92II	CATG	7 cut(s)	241, 260, 808, 887, 950, 982, 1000
HspAI	GCGC	1 cut(s)	845
KpnI	GGTACC	1 cut(s)	781
KroI	GCCGGC	1 cut(s)	1168
KroNI	GCCGGC	1 cut(s)	1170
Ksp22I	TGATCA	2 cut(s)	289, 681
Kzo9I	GATC	7 cut(s)	243, 283, 289, 371, 472, 681, 1200
LguI	GCTCTTC	1 cut(s)	124
LmnI	GCTCC	3 cut(s)	201, 221, 1020
Lsp1109I	GCAGC	1 cut(s)	408
LweI	GCATC	5 cut(s)	203, 393, 751, 760, 1168
MaeI	CTAG	2 cut(s)	737, 1262
MaeII	ACGT	2 cut(s)	11, 534
MaeIII	GTNAC	2 cut(s)	230, 868
MalI	GATC	7 cut(s)	245, 285, 291, 373, 474, 683, 1202
MboI	GATC	7 cut(s)	243, 283, 289, 371, 472, 681, 1200
MfeI	CAATTG	1 cut(s)	1247
MflI	RGATCY	1 cut(s)	371
MhlI	GDGCHC	2 cut(s)	210, 650
MluCI	AATT	8 cut(s)	136, 189, 420, 592, 903, 937, 1186, 1247
MlyI	GAGTC	5 cut(s)	14, 584, 631, 853, 929
MroNI	GCCGGC	1 cut(s)	1168
MseI	TTAA	4 cut(s)	266, 591, 746, 1270
MslI	CAYNNNNRTG	2 cut(s)	279, 1033
MspI	CCGG	6 cut(s)	210, 321, 334, 375, 632, 1169
MspR9I	CCNGG	5 cut(s)	200, 210, 321, 376, 632
MunI	CAATTG	1 cut(s)	1247
MvaI	CCWGG	1 cut(s)	200
MwoI	GCNNNNNNNGC	5 cut(s)	525, 605, 662, 770, 1165
NaeI	GCCGGC	1 cut(s)	1170
NciI	CCSGG	4 cut(s)	210, 321, 376, 632
NdeII	GATC	7 cut(s)	243, 283, 289, 371, 472, 681, 1200
NgoMIV	GCCGGC	1 cut(s)	1168
NheI	GCTAGC	1 cut(s)	736
NlaIII	CATG	7 cut(s)	241, 260, 808, 887, 950, 982, 1000
NlaIV	GGNNCC	5 cut(s)	197, 208, 332, 373, 779
NmuCI	GTSAC	1 cut(s)	868
NspI	RCATGY	1 cut(s)	260
PaeR7I	CTCGAG	1 cut(s)	391
PciSI	GCTCTTC	1 cut(s)	124
PdiI	GCCGGC	1 cut(s)	1170
PfeI	GAWTC	1 cut(s)	1045
PkrI	GCNGC	2 cut(s)	398, 1063
PleI	GAGTC	5 cut(s)	13, 583, 631, 853, 928
PpsI	GAGTC	5 cut(s)	13, 583, 631, 853, 928
Ppu21I	YACGTR	1 cut(s)	535
PsiI	TTATAA	2 cut(s)	188, 455
Psp6I	CCWGG	1 cut(s)	198
PspGI	CCWGG	1 cut(s)	198
PspN4I	GGNNCC	5 cut(s)	197, 208, 332, 373, 779
PspOMI	GGGCCC	1 cut(s)	206
PspPI	GGNCC	3 cut(s)	206, 207, 248
PspXI	VCTCGAGB	1 cut(s)	391
PsuI	RGATCY	1 cut(s)	371
RsaI	GTAC	4 cut(s)	533, 551, 779, 995
RsaNI	GTAC	4 cut(s)	532, 550, 778, 994
RseI	CAYNNNNRTG	2 cut(s)	279, 1033
SalI	GTCGAC	2 cut(s)	7, 13
SapI	GCTCTTC	1 cut(s)	124
SaqAI	TTAA	4 cut(s)	266, 591, 746, 1270
SatI	GCNGC	2 cut(s)	397, 1062
Sau3AI	GATC	7 cut(s)	243, 283, 289, 371, 472, 681, 1200
Sau96I	GGNCC	3 cut(s)	206, 207, 248
SchI	GAGTC	5 cut(s)	14, 584, 631, 853, 929
ScrFI	CCNGG	5 cut(s)	200, 210, 321, 376, 632
SduI	GDGCHC	2 cut(s)	210, 650
SfaNI	GCATC	5 cut(s)	203, 393, 751, 760, 1168
SfcI	CTRYAG	1 cut(s)	609
Sfr274I	CTCGAG	1 cut(s)	391
SlaI	CTCGAG	1 cut(s)	391
SmiMI	CAYNNNNRTG	2 cut(s)	279, 1033
SmlI	CTYRAG	2 cut(s)	391, 560
SmoI	CTYRAG	2 cut(s)	391, 560
Sse9I	AATT	8 cut(s)	136, 189, 420, 592, 903, 937, 1186, 1247
SsiI	CCGC	5 cut(s)	497, 509, 924, 1061, 1064
SspI	AATATT	1 cut(s)	480
SspMI	CTAG	2 cut(s)	737, 1262
StyD4I	CCNGG	5 cut(s)	198, 208, 319, 374, 630
StyI	CCWWGG	1 cut(s)	251
TaaI	ACNGT	1 cut(s)	100
TaiI	ACGT	2 cut(s)	14, 537
TaqI	TCGA	4 cut(s)	8, 14, 392, 1203
TasI	AATT	8 cut(s)	136, 189, 420, 592, 903, 937, 1186, 1247
TatI	WGTACW	1 cut(s)	993
TauI	GCSGC	1 cut(s)	1064
TfiI	GAWTC	1 cut(s)	1045
Tru1I	TTAA	4 cut(s)	266, 591, 746, 1270
Tru9I	TTAA	4 cut(s)	266, 591, 746, 1270
TscAI	CASTG	2 cut(s)	877, 933
TseFI	GTSAC	1 cut(s)	868
TseI	GCWGC	1 cut(s)	396
Tsp45I	GTSAC	1 cut(s)	868
TspDTI	ATGAA	8 cut(s)	37, 43, 49, 156, 226, 585, 903, 995
TspRI	CASTG	2 cut(s)	877, 933
XapI	RAATTY	2 cut(s)	420, 1186
XbaI	TCTAGA	1 cut(s)	1261
XceI	RCATGY	1 cut(s)	260
XcmI	CCANNNNNNNNNTGG	1 cut(s)	189
XhoI	CTCGAG	1 cut(s)	391
XmiI	GTMKAC	2 cut(s)	8, 14
XspI	CTAG	2 cut(s)	737, 1262
ZraI	GACGTC	1 cut(s)	12

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.