RLG00000008340

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0022296
Physical Location & Seq
Forward (+)
29150584 .. 29151239
656 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008340

Sequence Viewer

Length: 486 bp
ATGAATTTTACCAATAAATGGTTTATCACAGTTGCGTGTGTTGTCTTCCTCCTTCAACCAAGTTGTTCCACTTCCTTTGAATTTGATTCCATTACTCAAAATCAACCCATCAAGGATGGTGACGTTTTGGTCTCTTTCAGAAGAAAAGACAACCCTACTCCAGTCAGTGATACCTTCGGTCTCTTAGCAATCCATGGAAATGGAAGCTTGGTCATATACGGTAAGGATCAAAATACCCCTCTTTGGTCAGCTAATGTTAATCTCTCTTCACCAAACAGGTCCATTGCCAAGCTTTTGGATACAGGTAATCTTATTTTGCTTGAGAATGAGGGTAGCCAAATTGTACTTTGGGAAGGCTTTGATTATCCTTCAAATACTATGATTCCATCTATGAAACTAGGGTTCAAAAGGCGGTCTGGGTTGGGTTGGTTCTTAACGTCTTGGAAGTCTCAAGATGACCTGGGTACCGGGAGCTCTGTATACTAA

Protein Analysis

162

Amino Acids

17.88

Weight (kDa)

5.73

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 52 - 149 3.3e-25 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022296)

Species Orthologous Gene IDs
malus_domestica MD10G1308800.v1.1
rosa_laevigata RLG00000008340
rosa_roxburghii Rroxscaffold_3G00259830 Rroxscaffold_5G00353600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
Acc65I GGTACC 1 cut(s) 464
AccB1I GGYRCC 1 cut(s) 464
AccB7I CCANNNNNTGG 1 cut(s) 18
AccI GTMKAC 1 cut(s) 480
AciI CCGC 1 cut(s) 412
AclWI GGATC 1 cut(s) 234
AcsI RAATTY 2 cut(s) 4, 80
AfaI GTAC 2 cut(s) 345, 466
AfiI CCNNNNNNNGG 2 cut(s) 18, 243
AgsI TTSAA 4 cut(s) 56, 80, 372, 406
AjnI CCWGG 1 cut(s) 459
AluBI AGCT 4 cut(s) 207, 251, 292, 474
AluI AGCT 4 cut(s) 207, 251, 292, 474
Alw21I GWGCWC 1 cut(s) 476
Alw26I GTCTC 3 cut(s) 136, 185, 453
AlwI GGATC 1 cut(s) 234
ApoI RAATTY 2 cut(s) 4, 80
Asp718I GGTACC 1 cut(s) 464
AspS9I GGNCC 1 cut(s) 279
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 2 cut(s) 131, 261
AvaII GGWCC 1 cut(s) 279
BaeI ACNNNNGTAYC 2 cut(s) 448, 481
BanI GGYRCC 1 cut(s) 464
BanII GRGCYC 1 cut(s) 476
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 476
BccI CCATC 3 cut(s) 110, 116, 394
BciT130I CCWGG 1 cut(s) 461
BciVI GTATCC 1 cut(s) 292
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 3 cut(s) 136, 185, 453
BfaI CTAG 1 cut(s) 398
BfuI GTATCC 1 cut(s) 292
Bme1390I CCNGG 2 cut(s) 461, 469
Bme18I GGWCC 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 279
BmiI GGNNCC 1 cut(s) 466
BmrFI CCNGG 2 cut(s) 461, 469
BpiI GAAGAC 1 cut(s) 37
BpmI CTGGAG 1 cut(s) 144
BpuEI CTTGAG 2 cut(s) 341, 435
BpuMI CCSGG 1 cut(s) 469
BsaI GGTCTC 2 cut(s) 136, 185
BsaJI CCNNGG 2 cut(s) 193, 460
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 243
Bse1I ACTGG 1 cut(s) 161
Bse3DI GCAATG 1 cut(s) 282
BseBI CCWGG 1 cut(s) 461
BseDI CCNNGG 2 cut(s) 193, 460
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 2 cut(s) 18, 243
BseMI GCAATG 1 cut(s) 282
BseNI ACTGG 1 cut(s) 161
BshNI GGYRCC 1 cut(s) 464
BsiHKAI GWGCWC 1 cut(s) 476
BsiSI CCGG 1 cut(s) 468
BslI CCNNNNNNNGG 2 cut(s) 18, 243
BsmAI GTCTC 3 cut(s) 136, 185, 453
Bso31I GGTCTC 2 cut(s) 136, 185
Bsp1286I GDGCHC 1 cut(s) 476
Bsp143I GATC 1 cut(s) 226
Bsp19I CCATGG 1 cut(s) 193
BspACI CCGC 1 cut(s) 412
BspLI GGNNCC 1 cut(s) 466
BspPI GGATC 1 cut(s) 234
BspT107I GGYRCC 1 cut(s) 464
BspTNI GGTCTC 2 cut(s) 136, 185
BsrDI GCAATG 1 cut(s) 282
BsrI ACTGG 1 cut(s) 161
BssECI CCNNGG 2 cut(s) 193, 460
BssMI GATC 1 cut(s) 226
BssNAI GTATAC 1 cut(s) 481
BssT1I CCWWGG 1 cut(s) 193
Bst1107I GTATAC 1 cut(s) 481
Bst2UI CCWGG 1 cut(s) 461
Bst4CI ACNGT 2 cut(s) 31, 221
Bst6I CTCTTC 1 cut(s) 271
BstDEI CTNAG 1 cut(s) 184
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 1 cut(s) 229
BstMAI GTCTC 3 cut(s) 136, 185, 453
BstMBI GATC 1 cut(s) 226
BstNI CCWGG 1 cut(s) 461
BstSCI CCNGG 2 cut(s) 459, 467
BstV2I GAAGAC 1 cut(s) 37
BstXI CCANNNNNNTGG 2 cut(s) 200, 295
BstZ17I GTATAC 1 cut(s) 481
BsuI GTATCC 1 cut(s) 292
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 121
BtsIMutI CAGTG 1 cut(s) 172
Cfr13I GGNCC 1 cut(s) 279
Csp6I GTAC 2 cut(s) 344, 465
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviAII CATG 1 cut(s) 194
CviJI RGCY 6 cut(s) 207, 251, 292, 336, 357, 474
CviKI_1 RGCY 6 cut(s) 207, 251, 292, 336, 357, 474
CviQI GTAC 2 cut(s) 344, 465
DdeI CTNAG 1 cut(s) 184
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
DrdI GACNNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 128
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
Ecl136II GAGCTC 1 cut(s) 474
Eco130I CCWWGG 1 cut(s) 193
Eco24I GRGCYC 1 cut(s) 476
Eco31I GGTCTC 2 cut(s) 136, 185
Eco47I GGWCC 1 cut(s) 279
Eco53kI GAGCTC 1 cut(s) 474
EcoICRI GAGCTC 1 cut(s) 474
EcoRII CCWGG 1 cut(s) 459
EcoT14I CCWWGG 1 cut(s) 193
EcoT38I GRGCYC 1 cut(s) 476
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 1 cut(s) 197
FaiI YATR 6 cut(s) 195, 215, 217, 380, 392, 481
FatI CATG 1 cut(s) 193
FblI GTMKAC 1 cut(s) 480
FokI GGATG 1 cut(s) 128
FriOI GRGCYC 1 cut(s) 476
FspBI CTAG 1 cut(s) 398
GsuI CTGGAG 1 cut(s) 144
HapII CCGG 1 cut(s) 468
Hin1II CATG 1 cut(s) 197
HindIII AAGCTT 2 cut(s) 205, 290
HinfI GANTC 2 cut(s) 86, 382
HpaII CCGG 1 cut(s) 468
HphI GGTGA 2 cut(s) 131, 261
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 1 cut(s) 140
Hpy188III TCNNGA 1 cut(s) 452
Hpy8I GTNNAC 1 cut(s) 481
HpyAV CCTTC 4 cut(s) 62, 184, 347, 378
HpyCH4III ACNGT 2 cut(s) 31, 221
HpyCH4IV ACGT 2 cut(s) 123, 437
HpyF3I CTNAG 1 cut(s) 184
HpySE526I ACGT 2 cut(s) 123, 437
Hsp92II CATG 1 cut(s) 197
KpnI GGTACC 1 cut(s) 468
Kzo9I GATC 1 cut(s) 226
LmnI GCTCC 1 cut(s) 471
LpnPI CCDG 7 cut(s) 174, 262, 288, 402, 446, 473, 481
MaeI CTAG 1 cut(s) 398
MaeII ACGT 2 cut(s) 123, 437
MaeIII GTNAC 1 cut(s) 119
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MboII GAAGA 3 cut(s) 37, 153, 258
MhlI GDGCHC 1 cut(s) 476
MluCI AATT 3 cut(s) 4, 80, 339
MnlI CCTC 3 cut(s) 59, 249, 322
MseI TTAA 2 cut(s) 258, 434
MslI CAYNNNNRTG 1 cut(s) 198
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 2 cut(s) 461, 469
MvaI CCWGG 1 cut(s) 461
NciI CCSGG 1 cut(s) 469
NcoI CCATGG 1 cut(s) 193
NdeII GATC 1 cut(s) 226
NlaIII CATG 1 cut(s) 197
NlaIV GGNNCC 1 cut(s) 466
NmuCI GTSAC 1 cut(s) 119
PfeI GAWTC 2 cut(s) 86, 382
PflMI CCANNNNNTGG 1 cut(s) 18
Psp124BI GAGCTC 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 459
PspGI CCWGG 1 cut(s) 459
PspN4I GGNNCC 1 cut(s) 466
PspPI GGNCC 1 cut(s) 279
RsaI GTAC 2 cut(s) 345, 466
RsaNI GTAC 2 cut(s) 344, 465
RseI CAYNNNNRTG 1 cut(s) 198
SacI GAGCTC 1 cut(s) 476
SaqAI TTAA 2 cut(s) 258, 434
Sau3AI GATC 1 cut(s) 226
Sau96I GGNCC 1 cut(s) 279
ScrFI CCNGG 2 cut(s) 461, 469
SduI GDGCHC 1 cut(s) 476
SinI GGWCC 1 cut(s) 279
SmiMI CAYNNNNRTG 1 cut(s) 198
SmlI CTYRAG 2 cut(s) 320, 450
SmoI CTYRAG 2 cut(s) 320, 450
Sse9I AATT 3 cut(s) 4, 80, 339
SsiI CCGC 1 cut(s) 412
SspMI CTAG 1 cut(s) 398
SstI GAGCTC 1 cut(s) 476
StyD4I CCNGG 2 cut(s) 459, 467
StyI CCWWGG 1 cut(s) 193
TaaI ACNGT 2 cut(s) 31, 221
TaiI ACGT 2 cut(s) 126, 440
TaqII GACCGA 1 cut(s) 167
TasI AATT 3 cut(s) 4, 80, 339
TatI WGTACW 1 cut(s) 343
TfiI GAWTC 2 cut(s) 86, 382
Tru1I TTAA 2 cut(s) 258, 434
Tru9I TTAA 2 cut(s) 258, 434
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 1 cut(s) 119
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 2 cut(s) 17, 407
TspRI CASTG 1 cut(s) 172
Van91I CCANNNNNTGG 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 279
XapI RAATTY 2 cut(s) 4, 80
XmiI GTMKAC 1 cut(s) 480
XspI CTAG 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.