Rroxscaffold_3G00259830

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0022296
Physical Location & Seq
Reverse (-)
54214430 .. 54216497
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00259830.1

Sequence Viewer

Length: 540 bp
ATGAGTTTTCTCAAGGATTTGGCTCTCAAGGAGGCCTTTTTTGCACTTGGAGCCAGAGATTTTCTCACTTGGAGAGTTGGAATAGGATTTGCGACAAAGGATTTAAGTAATGTTCCTTGTATGGCAGATCAAACACCTTCAAGTATAGTGAGGCTGGCTAGAATTGTCGCCGCCCACGAGCACCTGATTGTTTTGCAAGAGCATTGCAGTAAAGTGGTACATCTATGCTATGGTGGTCGGGTTTTGGATCTCAAACCGATTTTCGATGGGATTTTTGGGTTTGCAAACAATCGAGTCGCGGCCCAATTTTCAGCTTGCGGTCTTGTGGATGTACCATTGATGATCAAAACATTTTCATCTGGGAGTGATATCATATCGCATATGACTCGATGCCCAAGCATTGTTTCCGAAACCCATTGTGGGATGAGCTTCATATATGTGCATGGCGATGATGCTCCTCCGAACAACCTCCCATCTGCAGTCACTGCCAAAAGCAGCAGCATCAAGATGGTTGAGGAGAGAGAACGTCAATATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

19.63

Weight (kDa)

7.03

Isoelectric Point (pI)

30.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022296)

Species Orthologous Gene IDs
malus_domestica MD10G1308800.v1.1
rosa_laevigata RLG00000008340
rosa_roxburghii Rroxscaffold_3G00259830 Rroxscaffold_5G00353600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 299
AciI CCGC 3 cut(s) 171, 299, 318
AclWI GGATC 1 cut(s) 255
AfaI GTAC 2 cut(s) 219, 333
AfiI CCNNNNNNNGG 1 cut(s) 420
AgsI TTSAA 1 cut(s) 141
AjuI GAANNNNNNNTTGG 2 cut(s) 388, 420
AluBI AGCT 2 cut(s) 314, 429
AluI AGCT 2 cut(s) 314, 429
Alw21I GWGCWC 1 cut(s) 183
AlwI GGATC 1 cut(s) 255
AlwNI CAGNNNCTG 1 cut(s) 485
AoxI GGCC 2 cut(s) 33, 300
ApeKI GCWGC 2 cut(s) 495, 498
AspS9I GGNCC 1 cut(s) 301
BauI CACGAG 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 2 cut(s) 507, 510
BccI CCATC 3 cut(s) 260, 481, 502
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BclI TGATCA 1 cut(s) 342
BfaI CTAG 1 cut(s) 159
BfmI CTRYAG 1 cut(s) 477
BisI GCNGC 4 cut(s) 171, 300, 496, 499
BlsI GCNGC 4 cut(s) 172, 301, 497, 500
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 1 cut(s) 52
BmsI GCATC 3 cut(s) 380, 442, 510
BplI GAGNNNNNCTC 2 cut(s) 48, 80
BpuEI CTTGAG 1 cut(s) 11
Bsc4I CCNNNNNNNGG 1 cut(s) 420
Bse3DI GCAATG 1 cut(s) 202
BseGI GGATG 2 cut(s) 334, 429
BseLI CCNNNNNNNGG 1 cut(s) 420
BseMI GCAATG 1 cut(s) 202
BseRI GAGGAG 2 cut(s) 447, 530
BseXI GCAGC 2 cut(s) 507, 510
Bsh1236I CGCG 1 cut(s) 299
BshFI GGCC 2 cut(s) 35, 302
BsiHKAI GWGCWC 1 cut(s) 183
BslI CCNNNNNNNGG 1 cut(s) 420
BsnI GGCC 2 cut(s) 35, 302
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 3 cut(s) 127, 247, 342
BspACI CCGC 3 cut(s) 171, 299, 318
BspANI GGCC 2 cut(s) 35, 302
BspFNI CGCG 1 cut(s) 299
BspLI GGNNCC 1 cut(s) 52
BspMAI CTGCAG 1 cut(s) 481
BspPI GGATC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 202
BssMI GATC 3 cut(s) 127, 247, 342
BssSI CACGAG 1 cut(s) 176
Bst2BI CACGAG 1 cut(s) 176
BstAPI GCANNNNNTGC 1 cut(s) 485
BstC8I GCNNGC 2 cut(s) 156, 316
BstF5I GGATG 2 cut(s) 334, 429
BstFNI CGCG 1 cut(s) 299
BstKTI GATC 3 cut(s) 130, 250, 345
BstMBI GATC 3 cut(s) 127, 247, 342
BstMWI GCNNNNNNNGC 3 cut(s) 41, 50, 485
BstSFI CTRYAG 1 cut(s) 477
BstUI CGCG 1 cut(s) 299
BstV1I GCAGC 2 cut(s) 507, 510
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuRI GGCC 2 cut(s) 35, 302
BtgZI GCGATG 1 cut(s) 462
BtsCI GGATG 2 cut(s) 334, 429
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 1 cut(s) 483
Cac8I GCNNGC 2 cut(s) 156, 316
CaiI CAGNNNCTG 1 cut(s) 485
Cfr13I GGNCC 1 cut(s) 301
Csp6I GTAC 2 cut(s) 218, 332
CviAII CATG 1 cut(s) 443
CviJI RGCY 8 cut(s) 23, 35, 53, 154, 158, 302, 314, 429
CviKI_1 RGCY 8 cut(s) 23, 35, 53, 154, 158, 302, 314, 429
CviQI GTAC 2 cut(s) 218, 332
DpnI GATC 3 cut(s) 129, 249, 344
DpnII GATC 3 cut(s) 127, 247, 342
Eco147I AGGCCT 1 cut(s) 35
Eco32I GATATC 1 cut(s) 370
EcoRV GATATC 1 cut(s) 370
FaeI CATG 1 cut(s) 446
FalI AAGNNNNNCTT 2 cut(s) 20, 52
FatI CATG 1 cut(s) 442
FauNDI CATATG 1 cut(s) 381
FbaI TGATCA 1 cut(s) 342
Fnu4HI GCNGC 4 cut(s) 171, 300, 496, 499
FokI GGATG 2 cut(s) 341, 436
Fsp4HI GCNGC 4 cut(s) 171, 300, 496, 499
FspBI CTAG 1 cut(s) 159
GluI GCNGC 4 cut(s) 171, 300, 496, 499
HaeIII GGCC 2 cut(s) 35, 302
Hin1II CATG 1 cut(s) 446
HinfI GANTC 2 cut(s) 294, 385
Hpy188I TCNGA 2 cut(s) 409, 462
Hpy188III TCNNGA 1 cut(s) 505
HpyAV CCTTC 1 cut(s) 147
HpyCH4IV ACGT 1 cut(s) 526
HpyCH4V TGCA 6 cut(s) 44, 196, 207, 284, 442, 479
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 50, 485
HpySE526I ACGT 1 cut(s) 526
Hsp92II CATG 1 cut(s) 446
Ksp22I TGATCA 1 cut(s) 342
Kzo9I GATC 3 cut(s) 127, 247, 342
LmnI GCTCC 2 cut(s) 50, 460
LpnPI CCDG 4 cut(s) 67, 140, 197, 345
Lsp1109I GCAGC 2 cut(s) 507, 510
LweI GCATC 3 cut(s) 380, 442, 510
MaeI CTAG 1 cut(s) 159
MaeII ACGT 1 cut(s) 526
MaeIII GTNAC 1 cut(s) 481
MalI GATC 3 cut(s) 129, 249, 344
MboI GATC 3 cut(s) 127, 247, 342
MflI RGATCY 1 cut(s) 247
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 3 cut(s) 162, 305, 535
MlyI GAGTC 2 cut(s) 303, 379
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 5 cut(s) 25, 144, 468, 479, 508
MseI TTAA 2 cut(s) 104, 538
MslI CAYNNNNRTG 3 cut(s) 437, 447, 506
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 3 cut(s) 41, 50, 485
NdeI CATATG 1 cut(s) 381
NdeII GATC 3 cut(s) 127, 247, 342
NlaIII CATG 1 cut(s) 446
NlaIV GGNNCC 1 cut(s) 52
NmuCI GTSAC 1 cut(s) 481
PceI AGGCCT 1 cut(s) 35
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PkrI GCNGC 4 cut(s) 172, 301, 497, 500
PleI GAGTC 2 cut(s) 302, 379
PpsI GAGTC 2 cut(s) 302, 379
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 301
PstI CTGCAG 1 cut(s) 481
PstNI CAGNNNCTG 1 cut(s) 485
PsuI RGATCY 1 cut(s) 247
RsaI GTAC 2 cut(s) 219, 333
RsaNI GTAC 2 cut(s) 218, 332
RseI CAYNNNNRTG 3 cut(s) 437, 447, 506
SaqAI TTAA 2 cut(s) 104, 538
SatI GCNGC 4 cut(s) 171, 300, 496, 499
Sau3AI GATC 3 cut(s) 127, 247, 342
Sau96I GGNCC 1 cut(s) 301
SchI GAGTC 2 cut(s) 303, 379
SduI GDGCHC 1 cut(s) 183
SetI ASST 6 cut(s) 139, 186, 316, 431, 471, 529
SfaNI GCATC 3 cut(s) 380, 442, 510
SfcI CTRYAG 1 cut(s) 477
SmiMI CAYNNNNRTG 3 cut(s) 437, 447, 506
SmlI CTYRAG 2 cut(s) 11, 26
SmoI CTYRAG 2 cut(s) 11, 26
Sse9I AATT 3 cut(s) 162, 305, 535
SseBI AGGCCT 1 cut(s) 35
SsiI CCGC 3 cut(s) 171, 299, 318
SspMI CTAG 1 cut(s) 159
StuI AGGCCT 1 cut(s) 35
TaiI ACGT 1 cut(s) 529
TaqI TCGA 3 cut(s) 264, 292, 388
TasI AATT 3 cut(s) 162, 305, 535
TauI GCSGC 2 cut(s) 173, 302
Tru1I TTAA 2 cut(s) 104, 538
Tru9I TTAA 2 cut(s) 104, 538
TscAI CASTG 1 cut(s) 490
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 2 cut(s) 495, 498
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 2 cut(s) 345, 421
TspRI CASTG 1 cut(s) 490
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.