RLG00000009008

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0019191
Physical Location & Seq
Forward (+)
44749950 .. 44757957
8008 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009008

Sequence Viewer

Length: 2175 bp
ATGGGAGACCCAGTAGTAGGGCCTGCTATGGGGACTCTCAAGATGTTTATAAAGGTTGGTACAGAGCTGTACAAGATTCATTCACAGTTCGAACGGCAGATGAAAAGGCTCATAGACACGCTGGAGATTTATGCACCAGTGATACAAAAGATTGAAAAATCGAACAACTCGAAGGATCTAGAGGGCCTCAAAGATCTTAAAAGTAAACTGGAGGAAGGCCGAATTCTCATTCACAGCTGTTTACAGGTTACAGCATCGCAATTAAAAAAAAAACATAGTTTCAACGGAGATCTGGATAAACTGATAGGAGATCTTTACCAAAGGTTAGCGGTGCTGCAAACATACGCAATAATCGCTATGAACAGAAACGAAGGTGCCGGCACAATTACAAGCTTCAGATGCGAACCCCCAGATCCTCCACTGTTTACAGTCGGACTGGATAAGCCTTTGCACGATCTGAAGAAGAATTTTCTTACTCGAGGAAAGTCGATTCTTGTGCTGACTGCTCCTGGAGGATTCGGGAAAACGACTTTGGCCACAGAGTTTTGCCATGATGAGAAAGTCAAAGATAAATTCCAGAACAATATCTTCTTTGTCCCTGTTCCAAAATATTCCTTGGACCATACGGCCCATGACGATCCGGAACAAGGAAAAAATATATTCTTGGACCATATTGTACAACAACTCCATCAGAGCATGCATTCCCGGCTGCCTGCTTCTTCTTCCCAAATCCAAGCAAATGCAGTTAATAAAGGGCTGCGCCAATTTCTGACAGAAGCAGGAGAAAAACCTTTATTGTTGGTCTTCGACGACGTTAGGCCTGGGTCGGAATTTCTTGTTGACAAGTTTCATGAAATCAAAATGCCAGAATACAAAATTTTGGTGACATCAAGATCTGACCTTCCGAGATTTGGTTCTTCATATTATTTGAAATCATTGAAAAAAGAAGATGCAAGGACTTGTTTTTATCATTTCCTGGAAAACAAGAGCTGCGATATTCCAACTGATCTTAAAGAGAGGATACTGGAGCATTGTAAGGGACTTCCCCTAGTCATTGAAGTGGTTGTATCGTCACTTCTGCGTGAGAAGGCTGTACAGACATGGAAAACAATGGACGCCAAGTGGTCTCGCGGTTCCATTCTTGATTCCGTTACTGATTTGTTTAACCGCCTCCAAACCAGCGTAGATGCCTTGGTTGGAAATCAAGCAATCCTAAAACAATGCTTCATAGACCTTGGTGTTTTTCTTAAAGACCAAACTATCCCAGTTGCTGCCCTTATTGATATGTGGACAGAGTTACATAAGCTAGAAGGTGAAGACGATGAGAGCATTGCAAACCTCCACGAGCTCACTGAGAGGAGTTTTGCTAATCTTGTATTCCCAAGGAGAGATAAGACGGAAGTGGCGGAGGATGATAGCTACTATAGTCAACACTTTGTTGCTCAGCATGATATACTAAGAGACTTGGCCATCCGTCAGAGCAATGAGGCTGCAGACAGACTCATAATAGACGTGCCAAACAAATCTTTTCCCCTGTGGTGGACGGAAGGAATGTATGAGCAACCCATCAACGCTCGCCTAATATCTATCTCAACTGAGGGAGTATTCCCAAAAGAATGGGACAAAATGAAGCTACCAAAAGCTGAGGTTCTTGTTCTGCACTTCCAGGCAGAGAGCTACGCCTTACCCAACTTCCTCAAGACAATGTCAAGCTCATTGAAGGTTCTCATAGTCACAAATCATGGTCTCTTGCATGCCAAAGAACTGCCTGCCAAGCTCTGTGGTCTTGTAAGTTTAAAGAAGCTCAGTATTACCAACTGTGAGAAACTACATGCCTTGCCTGAAGAGATTGGAGATCTGGTCAATTTAGAAGTGCTAAGGCTATTGGCTTGCATAAAATTGTTACAGTTGCCTGGCTCGATTAGCAAACTCACAGGCTTAAAACTTTTTGACTTGTCTGATTGCTGGAGCATTGAAGAGTTGTCTGACATTGGTGAAATGCACTTTTTGAAAAAACTCGGAATGAGACACTGCCCAGGGTTGAGAACGCTCCCTCAATCAATTTCGAATTTGCACCAGGAGATGGATGTGATATGTGATGAAAAGACAGAAAAGTTATGGGATAACATCCGCTATGGCAGTAACATTCACATAATTGTCGAGAAACTTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

725

Amino Acids

82.17

Weight (kDa)

6.36

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 105 2.3e-10 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 152 - 299 5.1e-06 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019191)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15380 FvH4_1g15380 FvH4_1g15380 FvH4_1g15380
rosa_laevigata RLG00000009008
rosa_multiflora Rmu_sc0002316.1_g000060
rosa_roxburghii Rroxscaffold_5G00347890
rosa_samantha Rh3BG268900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 50
AccB1I GGYRCC 1 cut(s) 374
AccB7I CCANNNNNTGG 1 cut(s) 2083
AccII CGCG 1 cut(s) 1131
AccIII TCCGGA 1 cut(s) 640
AciI CCGC 5 cut(s) 329, 1131, 1168, 1406, 2131
AclWI GGATC 3 cut(s) 183, 407, 632
AcoI YGGCCR 2 cut(s) 534, 1467
AcsI RAATTY 6 cut(s) 222, 466, 572, 830, 876, 2068
AcuI CTGAAG 3 cut(s) 379, 479, 1863
AcyI GRCGYC 1 cut(s) 1116
AfaI GTAC 4 cut(s) 61, 71, 678, 1095
AfiI CCNNNNNNNGG 6 cut(s) 17, 29, 647, 911, 1539, 2083
AgsI TTSAA 9 cut(s) 155, 283, 931, 940, 1058, 1720, 1976, 2011, 2171
AjiI CACGTC 1 cut(s) 1513
AjnI CCWGG 7 cut(s) 508, 820, 975, 1665, 1912, 2035, 2076
AjuI GAANNNNNNNTTGG 2 cut(s) 515, 547
Alw21I GWGCWC 1 cut(s) 1350
Alw26I GTCTC 4 cut(s) 1131, 1455, 1751, 2020
AlwI GGATC 3 cut(s) 183, 407, 632
AlwNI CAGNNNCTG 1 cut(s) 1271
Ama87I CYCGRG 1 cut(s) 477
Aor13HI TCCGGA 1 cut(s) 640
AoxI GGCC 7 cut(s) 20, 184, 217, 534, 627, 818, 1467
ApeKI GCWGC 6 cut(s) 334, 709, 757, 990, 1271, 1490
ApoI RAATTY 6 cut(s) 222, 466, 572, 830, 876, 2068
AspLEI GCGC 1 cut(s) 762
AspS9I GGNCC 5 cut(s) 20, 184, 619, 628, 667
AsuC2I CCSGG 1 cut(s) 706
AsuHPI GGTGA 3 cut(s) 895, 1325, 2006
AsuII TTCGAA 2 cut(s) 90, 2066
AvaI CYCGRG 1 cut(s) 477
AvaII GGWCC 2 cut(s) 619, 667
BalI TGGCCA 2 cut(s) 536, 1469
BanI GGYRCC 1 cut(s) 374
BanII GRGCYC 1 cut(s) 1350
BarI GAAGNNNNNNTAC 2 cut(s) 1059, 1091
BauI CACGAG 1 cut(s) 1343
BbsI GAAGAC 2 cut(s) 796, 1323
Bbv12I GWGCWC 1 cut(s) 1350
BbvCI CCTCAGC 1 cut(s) 1644
BbvI GCAGC 6 cut(s) 321, 696, 744, 977, 1258, 1477
BccI CCATC 4 cut(s) 696, 1478, 1574, 2077
BceAI ACGGC 2 cut(s) 110, 642
BcgI CGANNNNNNTGC 2 cut(s) 478, 512
BciT130I CCWGG 7 cut(s) 510, 822, 977, 1667, 1914, 2037, 2078
BciVI GTATCC 1 cut(s) 1014
BcnI CCSGG 1 cut(s) 706
BcoDI GTCTC 4 cut(s) 1131, 1455, 1751, 2020
BfaI CTAG 3 cut(s) 179, 1049, 1307
BfmI CTRYAG 2 cut(s) 1423, 1491
BfuI GTATCC 1 cut(s) 1014
BglII AGATCT 5 cut(s) 193, 289, 310, 893, 1855
BisI GCNGC 6 cut(s) 335, 710, 758, 991, 1272, 1491
BlpI GCTNAGC 1 cut(s) 1443
BlsI GCNGC 6 cut(s) 336, 711, 759, 992, 1273, 1492
Bme1390I CCNGG 8 cut(s) 510, 706, 822, 977, 1667, 1914, 2037, 2078
Bme18I GGWCC 2 cut(s) 619, 667
BmeT110I CYCGRG 1 cut(s) 477
BmgBI CACGTC 1 cut(s) 1513
BmgT120I GGNCC 5 cut(s) 20, 184, 619, 628, 667
BmiI GGNNCC 2 cut(s) 376, 1135
BmrFI CCNGG 8 cut(s) 510, 706, 822, 977, 1667, 1914, 2037, 2078
BmrI ACTGGG 2 cut(s) 5, 1259
BmsI GCATC 4 cut(s) 263, 389, 940, 1177
BmuI ACTGGG 2 cut(s) 5, 1259
BpiI GAAGAC 2 cut(s) 796, 1323
BpmI CTGGAG 5 cut(s) 143, 230, 531, 1046, 1987
Bpu10I CCTNAGC 2 cut(s) 1644, 1877
Bpu1102I GCTNAGC 1 cut(s) 1443
Bpu14I TTCGAA 2 cut(s) 90, 2066
BpuEI CTTGAG 2 cut(s) 23, 1682
BpuMI CCSGG 1 cut(s) 706
BsaHI GRCGYC 1 cut(s) 1116
BsaI GGTCTC 2 cut(s) 1131, 1751
BsaJI CCNNGG 7 cut(s) 615, 821, 1191, 1234, 1382, 2035, 2036
BsaWI WCCGGW 1 cut(s) 640
BsaXI ACNNNNNCTCC 5 cut(s) 27, 669, 699, 1845, 1875
Bsc4I CCNNNNNNNGG 6 cut(s) 17, 29, 647, 911, 1539, 2083
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 6 cut(s) 11, 137, 213, 441, 1029, 1265
Bse3DI GCAATG 2 cut(s) 1329, 1489
BseAI TCCGGA 1 cut(s) 640
BseBI CCWGG 7 cut(s) 510, 822, 977, 1667, 1914, 2037, 2078
BseDI CCNNGG 7 cut(s) 615, 821, 1191, 1234, 1382, 2035, 2036
BseGI GGATG 4 cut(s) 1417, 1470, 2092, 2127
BseLI CCNNNNNNNGG 6 cut(s) 17, 29, 647, 911, 1539, 2083
BseMI GCAATG 2 cut(s) 1329, 1489
BseMII CTCAG 5 cut(s) 1344, 1457, 1587, 1635, 1819
BseNI ACTGG 6 cut(s) 11, 137, 213, 441, 1029, 1265
BseRI GAGGAG 1 cut(s) 1372
BseXI GCAGC 6 cut(s) 321, 696, 744, 977, 1258, 1477
BsgI GTGCAG 1 cut(s) 1643
Bsh1236I CGCG 1 cut(s) 1131
BshFI GGCC 7 cut(s) 22, 186, 219, 536, 629, 820, 1469
BshNI GGYRCC 1 cut(s) 374
BsiHKAI GWGCWC 1 cut(s) 1350
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 3 cut(s) 378, 641, 706
BslFI GGGAC 4 cut(s) 46, 581, 1053, 1634
BslI CCNNNNNNNGG 6 cut(s) 17, 29, 647, 911, 1539, 2083
BsmAI GTCTC 4 cut(s) 1131, 1455, 1751, 2020
BsmFI GGGAC 4 cut(s) 46, 581, 1053, 1634
BsmI GAATGC 1 cut(s) 700
BsnI GGCC 7 cut(s) 22, 186, 219, 536, 629, 820, 1469
Bso31I GGTCTC 2 cut(s) 1131, 1751
BsoBI CYCGRG 1 cut(s) 477
Bsp119I TTCGAA 2 cut(s) 90, 2066
Bsp1286I GDGCHC 1 cut(s) 1350
Bsp13I TCCGGA 1 cut(s) 640
Bsp1407I TGTACA 3 cut(s) 69, 676, 1093
Bsp1720I GCTNAGC 1 cut(s) 1443
BspACI CCGC 5 cut(s) 329, 1131, 1168, 1406, 2131
BspANI GGCC 7 cut(s) 22, 186, 219, 536, 629, 820, 1469
BspCNI CTCAG 5 cut(s) 1345, 1456, 1588, 1636, 1818
BspEI TCCGGA 1 cut(s) 640
BspFNI CGCG 1 cut(s) 1131
BspHI TCATGA 1 cut(s) 850
BspLI GGNNCC 2 cut(s) 376, 1135
BspMAI CTGCAG 1 cut(s) 1495
BspPI GGATC 3 cut(s) 183, 407, 632
BspT104I TTCGAA 2 cut(s) 90, 2066
BspT107I GGYRCC 1 cut(s) 374
BspTNI GGTCTC 2 cut(s) 1131, 1751
BsrDI GCAATG 2 cut(s) 1329, 1489
BsrFI RCCGGY 1 cut(s) 377
BsrGI TGTACA 3 cut(s) 69, 676, 1093
BsrI ACTGG 6 cut(s) 11, 137, 213, 441, 1029, 1265
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 7 cut(s) 615, 821, 1191, 1234, 1382, 2035, 2036
BssNI GRCGYC 1 cut(s) 1116
BssSI CACGAG 1 cut(s) 1343
BssT1I CCWWGG 4 cut(s) 615, 1191, 1234, 1382
Bst2BI CACGAG 1 cut(s) 1343
Bst2UI CCWGG 7 cut(s) 510, 822, 977, 1667, 1914, 2037, 2078
Bst4CI ACNGT 5 cut(s) 87, 423, 430, 1820, 1908
Bst6I CTCTTC 2 cut(s) 1839, 1971
BstACI GRCGYC 1 cut(s) 1116
BstAUI TGTACA 3 cut(s) 69, 676, 1093
BstBI TTCGAA 2 cut(s) 90, 2066
BstC8I GCNNGC 8 cut(s) 24, 379, 698, 714, 1576, 1755, 1770, 1891
BstDEI CTNAG 7 cut(s) 1353, 1443, 1457, 1596, 1644, 1805, 1877
BstF5I GGATG 4 cut(s) 1417, 1470, 2092, 2127
BstFNI CGCG 1 cut(s) 1131
BstHHI GCGC 1 cut(s) 762
BstMAI GTCTC 4 cut(s) 1131, 1455, 1751, 2020
BstMWI GCNNNNNNNGC 5 cut(s) 353, 399, 706, 1774, 1923
BstNI CCWGG 7 cut(s) 510, 822, 977, 1667, 1914, 2037, 2078
BstNSI RCATGY 3 cut(s) 700, 1757, 1835
BstSCI CCNGG 8 cut(s) 508, 704, 820, 975, 1665, 1912, 2035, 2076
BstSFI CTRYAG 2 cut(s) 1423, 1491
BstUI CGCG 1 cut(s) 1131
BstV1I GCAGC 6 cut(s) 321, 696, 744, 977, 1258, 1477
BstV2I GAAGAC 2 cut(s) 796, 1323
BstX2I RGATCY 7 cut(s) 175, 193, 289, 310, 412, 893, 1855
BstXI CCANNNNNNTGG 1 cut(s) 1617
BstYI RGATCY 7 cut(s) 175, 193, 289, 310, 412, 893, 1855
BsuI GTATCC 1 cut(s) 1014
BsuRI GGCC 7 cut(s) 22, 186, 219, 536, 629, 820, 1469
BtgZI GCGATG 1 cut(s) 240
BtrI CACGTC 1 cut(s) 1513
BtsCI GGATG 4 cut(s) 1417, 1470, 2092, 2127
BtsI GCAGTG 1 cut(s) 2029
BtsIMutI CAGTG 4 cut(s) 144, 419, 1350, 2029
Cac8I GCNNGC 8 cut(s) 24, 379, 698, 714, 1576, 1755, 1770, 1891
CaiI CAGNNNCTG 1 cut(s) 1271
CciI TCATGA 1 cut(s) 850
CfoI GCGC 1 cut(s) 762
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 5 cut(s) 20, 184, 619, 628, 667
CseI GACGC 1 cut(s) 1124
Csp6I GTAC 4 cut(s) 60, 70, 677, 1094
CspCI CAANNNNNGTGG 2 cut(s) 1762, 1797
CviAII CATG 9 cut(s) 551, 632, 697, 851, 1101, 1448, 1742, 1754, 1832
CviQI GTAC 4 cut(s) 60, 70, 677, 1094
DdeI CTNAG 7 cut(s) 1353, 1443, 1457, 1596, 1644, 1805, 1877
DraI TTTAAA 1 cut(s) 1797
EaeI YGGCCR 2 cut(s) 534, 1467
Eam1104I CTCTTC 2 cut(s) 1839, 1971
EarI CTCTTC 2 cut(s) 1839, 1971
EciI GGCGGA 1 cut(s) 1421
Ecl136II GAGCTC 1 cut(s) 1348
Eco130I CCWWGG 4 cut(s) 615, 1191, 1234, 1382
Eco147I AGGCCT 1 cut(s) 820
Eco24I GRGCYC 1 cut(s) 1350
Eco31I GGTCTC 2 cut(s) 1131, 1751
Eco47I GGWCC 2 cut(s) 619, 667
Eco53kI GAGCTC 1 cut(s) 1348
Eco57I CTGAAG 3 cut(s) 379, 479, 1863
Eco88I CYCGRG 1 cut(s) 477
EcoICRI GAGCTC 1 cut(s) 1348
EcoO109I RGGNCCY 2 cut(s) 20, 184
EcoRI GAATTC 1 cut(s) 222
EcoRII CCWGG 7 cut(s) 508, 820, 975, 1665, 1912, 2035, 2076
EcoT14I CCWWGG 4 cut(s) 615, 1191, 1234, 1382
EcoT22I ATGCAT 1 cut(s) 702
EcoT38I GRGCYC 1 cut(s) 1350
ErhI CCWWGG 4 cut(s) 615, 1191, 1234, 1382
FaeI CATG 9 cut(s) 554, 635, 700, 854, 1104, 1451, 1745, 1757, 1835
FaqI GGGAC 4 cut(s) 46, 581, 1053, 1634
FatI CATG 9 cut(s) 550, 631, 696, 850, 1100, 1447, 1741, 1753, 1831
Fnu4HI GCNGC 6 cut(s) 335, 710, 758, 991, 1272, 1491
FokI GGATG 4 cut(s) 1424, 1457, 2099, 2114
FriOI GRGCYC 1 cut(s) 1350
Fsp4HI GCNGC 6 cut(s) 335, 710, 758, 991, 1272, 1491
FspBI CTAG 3 cut(s) 179, 1049, 1307
GlaI GCGC 1 cut(s) 761
GluI GCNGC 6 cut(s) 335, 710, 758, 991, 1272, 1491
GsuI CTGGAG 5 cut(s) 143, 230, 531, 1046, 1987
HaeIII GGCC 7 cut(s) 22, 186, 219, 536, 629, 820, 1469
HapII CCGG 3 cut(s) 378, 641, 706
HgaI GACGC 1 cut(s) 1124
HhaI GCGC 1 cut(s) 762
Hin1I GRCGYC 1 cut(s) 1116
Hin1II CATG 9 cut(s) 554, 635, 700, 854, 1104, 1451, 1745, 1757, 1835
Hin6I GCGC 1 cut(s) 760
HinP1I GCGC 1 cut(s) 760
HincII GTYRAC 2 cut(s) 841, 1430
HindII GTYRAC 2 cut(s) 841, 1430
HindIII AAGCTT 1 cut(s) 391
HinfI GANTC 6 cut(s) 34, 76, 490, 516, 1145, 1500
HpaII CCGG 3 cut(s) 378, 641, 706
HphI GGTGA 3 cut(s) 895, 1325, 2006
Hpy166II GTNNAC 7 cut(s) 206, 242, 426, 841, 1290, 1430, 1542
Hpy8I GTNNAC 7 cut(s) 206, 242, 426, 841, 1290, 1430, 1542
Hpy99I CGWCG 2 cut(s) 812, 815
HpyAV CCTTC 8 cut(s) 166, 209, 365, 911, 1081, 1304, 1541, 1714
HpyCH4III ACNGT 5 cut(s) 87, 423, 430, 1820, 1908
HpyCH4IV ACGT 2 cut(s) 813, 1512
HpyF10VI GCNNNNNNNGC 5 cut(s) 353, 399, 706, 1774, 1923
HpyF3I CTNAG 7 cut(s) 1353, 1443, 1457, 1596, 1644, 1805, 1877
HpySE526I ACGT 2 cut(s) 813, 1512
Hsp92I GRCGYC 1 cut(s) 1116
Hsp92II CATG 9 cut(s) 554, 635, 700, 854, 1104, 1451, 1745, 1757, 1835
HspAI GCGC 1 cut(s) 760
Kpn2I TCCGGA 1 cut(s) 640
KroI GCCGGC 1 cut(s) 377
KroNI GCCGGC 1 cut(s) 379
LmnI GCTCC 4 cut(s) 511, 1027, 1968, 2055
Lsp1109I GCAGC 6 cut(s) 321, 696, 744, 977, 1258, 1477
LweI GCATC 4 cut(s) 263, 389, 940, 1177
MaeI CTAG 3 cut(s) 179, 1049, 1307
MaeII ACGT 2 cut(s) 813, 1512
MaeIII GTNAC 8 cut(s) 247, 883, 1071, 1150, 1296, 1732, 1902, 2141
MflI RGATCY 7 cut(s) 175, 193, 289, 310, 412, 893, 1855
MhlI GDGCHC 1 cut(s) 1350
MlsI TGGCCA 2 cut(s) 536, 1469
MluNI TGGCCA 2 cut(s) 536, 1469
MlyI GAGTC 2 cut(s) 28, 1494
MmeI TCCRAC 4 cut(s) 412, 807, 1025, 1177
Mox20I TGGCCA 2 cut(s) 536, 1469
Mph1103I ATGCAT 1 cut(s) 702
MroI TCCGGA 1 cut(s) 640
MroNI GCCGGC 1 cut(s) 377
MscI TGGCCA 2 cut(s) 536, 1469
MseI TTAA 8 cut(s) 198, 263, 747, 1011, 1164, 1248, 1796, 1940
MslI CAYNNNNRTG 1 cut(s) 1058
Msp20I TGGCCA 2 cut(s) 536, 1469
MspA1I CMGCKG 1 cut(s) 237
MspI CCGG 3 cut(s) 378, 641, 706
MspR9I CCNGG 8 cut(s) 510, 706, 822, 977, 1667, 1914, 2037, 2078
Mva1269I GAATGC 1 cut(s) 700
MvaI CCWGG 7 cut(s) 510, 822, 977, 1667, 1914, 2037, 2078
MvnI CGCG 1 cut(s) 1131
MwoI GCNNNNNNNGC 5 cut(s) 353, 399, 706, 1774, 1923
NaeI GCCGGC 1 cut(s) 379
NciI CCSGG 1 cut(s) 706
NgoMIV GCCGGC 1 cut(s) 377
NlaIII CATG 9 cut(s) 554, 635, 700, 854, 1104, 1451, 1745, 1757, 1835
NlaIV GGNNCC 2 cut(s) 376, 1135
NmuCI GTSAC 3 cut(s) 883, 1071, 1732
NsiI ATGCAT 1 cut(s) 702
NspI RCATGY 3 cut(s) 700, 1757, 1835
NspV TTCGAA 2 cut(s) 90, 2066
PaeI GCATGC 2 cut(s) 700, 1757
PaeR7I CTCGAG 1 cut(s) 477
PagI TCATGA 1 cut(s) 850
PasI CCCWGGG 1 cut(s) 2036
PceI AGGCCT 1 cut(s) 820
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PctI GAATGC 1 cut(s) 700
PdiI GCCGGC 1 cut(s) 379
PfeI GAWTC 4 cut(s) 76, 490, 516, 1145
PflFI GACNNNGTC 1 cut(s) 1705
PflMI CCANNNNNTGG 1 cut(s) 2083
PfoI TCCNGGA 2 cut(s) 508, 975
PkrI GCNGC 6 cut(s) 336, 711, 759, 992, 1273, 1492
PleI GAGTC 2 cut(s) 28, 1494
PpsI GAGTC 2 cut(s) 28, 1494
PsiI TTATAA 1 cut(s) 50
Psp124BI GAGCTC 1 cut(s) 1350
Psp6I CCWGG 7 cut(s) 508, 820, 975, 1665, 1912, 2035, 2076
PspGI CCWGG 7 cut(s) 508, 820, 975, 1665, 1912, 2035, 2076
PspN4I GGNNCC 2 cut(s) 376, 1135
PspPI GGNCC 5 cut(s) 20, 184, 619, 628, 667
PspXI VCTCGAGB 1 cut(s) 477
PstI CTGCAG 1 cut(s) 1495
PstNI CAGNNNCTG 1 cut(s) 1271
PsuI RGATCY 7 cut(s) 175, 193, 289, 310, 412, 893, 1855
PsyI GACNNNGTC 1 cut(s) 1705
PvuII CAGCTG 1 cut(s) 237
RsaI GTAC 4 cut(s) 61, 71, 678, 1095
RsaNI GTAC 4 cut(s) 60, 70, 677, 1094
RseI CAYNNNNRTG 1 cut(s) 1058
SacI GAGCTC 1 cut(s) 1350
SaqAI TTAA 8 cut(s) 198, 263, 747, 1011, 1164, 1248, 1796, 1940
SatI GCNGC 6 cut(s) 335, 710, 758, 991, 1272, 1491
Sau96I GGNCC 5 cut(s) 20, 184, 619, 628, 667
SchI GAGTC 2 cut(s) 28, 1494
ScrFI CCNGG 8 cut(s) 510, 706, 822, 977, 1667, 1914, 2037, 2078
SduI GDGCHC 1 cut(s) 1350
SfaNI GCATC 4 cut(s) 263, 389, 940, 1177
SfcI CTRYAG 2 cut(s) 1423, 1491
Sfr274I CTCGAG 1 cut(s) 477
SfuI TTCGAA 2 cut(s) 90, 2066
SinI GGWCC 2 cut(s) 619, 667
SlaI CTCGAG 1 cut(s) 477
SmiMI CAYNNNNRTG 1 cut(s) 1058
SmlI CTYRAG 3 cut(s) 38, 477, 1697
SmoI CTYRAG 3 cut(s) 38, 477, 1697
SphI GCATGC 2 cut(s) 700, 1757
SseBI AGGCCT 1 cut(s) 820
SsiI CCGC 5 cut(s) 329, 1131, 1168, 1406, 2131
SspI AATATT 1 cut(s) 611
SspMI CTAG 3 cut(s) 179, 1049, 1307
SstI GAGCTC 1 cut(s) 1350
StuI AGGCCT 1 cut(s) 820
StyD4I CCNGG 8 cut(s) 508, 704, 820, 975, 1665, 1912, 2035, 2076
StyI CCWWGG 4 cut(s) 615, 1191, 1234, 1382
TaaI ACNGT 5 cut(s) 87, 423, 430, 1820, 1908
TaiI ACGT 2 cut(s) 816, 1515
TaqI TCGA 9 cut(s) 90, 161, 170, 478, 488, 807, 1919, 2066, 2160
TatI WGTACW 3 cut(s) 69, 676, 1093
TfiI GAWTC 4 cut(s) 76, 490, 516, 1145
Tru1I TTAA 8 cut(s) 198, 263, 747, 1011, 1164, 1248, 1796, 1940
Tru9I TTAA 8 cut(s) 198, 263, 747, 1011, 1164, 1248, 1796, 1940
TscAI CASTG 4 cut(s) 144, 426, 1357, 2036
TseFI GTSAC 3 cut(s) 883, 1071, 1732
TseI GCWGC 6 cut(s) 334, 709, 757, 990, 1271, 1490
Tsp45I GTSAC 3 cut(s) 883, 1071, 1732
TspDTI ATGAA 9 cut(s) 68, 116, 374, 839, 867, 909, 1216, 1643, 2115
TspGWI ACGGA 5 cut(s) 300, 1138, 1412, 1463, 1559
TspRI CASTG 4 cut(s) 144, 426, 1357, 2036
Tth111I GACNNNGTC 1 cut(s) 1705
Van91I CCANNNNNTGG 1 cut(s) 2083
VpaK11BI GGWCC 2 cut(s) 619, 667
XapI RAATTY 6 cut(s) 222, 466, 572, 830, 876, 2068
XbaI TCTAGA 1 cut(s) 178
XceI RCATGY 3 cut(s) 700, 1757, 1835
XhoI CTCGAG 1 cut(s) 477
XspI CTAG 3 cut(s) 179, 1049, 1307
Zsp2I ATGCAT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.