RLG00000009502

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0017282
Physical Location & Seq
Forward (+)
53703090 .. 53705715
2626 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009502

Sequence Viewer

Length: 855 bp
ATGTTATGCTCATCCGAAGAGCTTGAATATAAATTCTTACTCAGAGTACATGTTATCAATGGCCTGCCTCAGAATTCGAAGCCTGCGAATATAGAATGCAAGTCGACAGATACGTATATTGGCGAACGCAACCTGAATGTTGGTGACGACTTCACATGGAATGCCAAGGAACGCTCTCTGTATTACTGTCGAGCATTCTGGGGTAGATTTTTTGCATCCTGGCATGCAGTTCAGCCTAAGCGAGATGATGGTAAAGCAGCAGTGTTTTGGCTGTCTATTAATGACCATGCGGCTTGGTGCATGAATACCTGTACTGCTGGACTCGGTGTGATTGTGCGTGTGCGTGACTGCCAAGGAAAATTCCTTGCAGCAGAGGCTAAAACTGACCGTTGCAGTTCTGCCTTGATCGCTGCAGCTAAAACAGTGATTGCGGGTCTTGACTTGGGCAATTGCGGGCATATGATGCTAATCCATGTGATCATCTTCTCCACCACCATTTCCATCAGTTCTTCATCTCCAGCAAGACTCTTCGACAGTGGCCAACTTCCGGTGCTTAATTTCATTAATGGGTTGCCGAAAAACTCAGAGCCATGGGAGCTCAGATGCGGTAATCAGAGCACTAACGAACACCATGCGTATTTGAAAACTGGTACAGAGGACCAATGGAGGATCGAGAAGAACGTTGGTTACAGCTGCGATACACTGTGGGGAAATCAGTTTGCATACTGGAAAGTTTATCAGCCTGGTGAAGACTCCGGCAAAGCCACAGTGTTCTGGTTGGCTAAAGACGATGGTTTTTATCGCAGCTGGGATAAATCTAGGTGGACAAGAGAGTTTGAATGGGAACATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

285

Amino Acids

32.35

Weight (kDa)

6.45

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 15 - 90 4.6e-08 Plant self-incompatibility protein S1
Self-incomp_S1 PF05938 186 - 281 2.6e-08 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017282)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g05643
rosa_chinensis RchiOBHm_Chr4g0396821
rosa_laevigata RLG00000009502
rosa_multiflora Rmu_sc0000487.1_g000053
rosa_roxburghii Rroxscaffold_5G00341660
rosa_rugosa Rorug03G0347500
rosa_samantha Rh4AG072000 Rh4AG072800 Rh4AG074900 Rh4BG069900 Rh4CG078200 Rh4DG067700
rosa_wichuraiana Rw4G005910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 104
AciI CCGC 4 cut(s) 290, 431, 453, 606
AclI AACGTT 1 cut(s) 681
AclWI GGATC 1 cut(s) 677
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 3 cut(s) 32, 73, 359
AfaI GTAC 3 cut(s) 48, 313, 652
AfiI CCNNNNNNNGG 1 cut(s) 547
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 3 cut(s) 26, 643, 839
AjnI CCWGG 2 cut(s) 218, 742
AluBI AGCT 5 cut(s) 22, 416, 598, 693, 807
AluI AGCT 5 cut(s) 22, 416, 598, 693, 807
Alw21I GWGCWC 2 cut(s) 600, 620
AlwI GGATC 1 cut(s) 677
AoxI GGCC 2 cut(s) 61, 538
ApeKI GCWGC 6 cut(s) 257, 368, 410, 413, 693, 804
ApoI RAATTY 3 cut(s) 32, 73, 359
AseI ATTAAT 2 cut(s) 279, 564
AspS9I GGNCC 1 cut(s) 658
AsuHPI GGTGA 2 cut(s) 155, 758
AsuII TTCGAA 1 cut(s) 77
AvaII GGWCC 1 cut(s) 658
BalI TGGCCA 1 cut(s) 540
BanII GRGCYC 1 cut(s) 600
BbsI GAAGAC 1 cut(s) 756
Bbv12I GWGCWC 2 cut(s) 600, 620
BbvI GCAGC 6 cut(s) 269, 380, 397, 425, 680, 816
BccI CCATC 3 cut(s) 242, 509, 785
BcgI CGANNNNNNTGC 2 cut(s) 614, 648
BciT130I CCWGG 2 cut(s) 220, 744
BclI TGATCA 1 cut(s) 477
BfaI CTAG 1 cut(s) 819
BfmI CTRYAG 1 cut(s) 411
BisI GCNGC 7 cut(s) 258, 291, 369, 411, 414, 694, 805
BlsI GCNGC 7 cut(s) 259, 292, 370, 412, 415, 695, 806
Bme1390I CCNGG 2 cut(s) 220, 744
Bme18I GGWCC 1 cut(s) 658
BmgT120I GGNCC 1 cut(s) 658
BmrFI CCNGG 2 cut(s) 220, 744
BmsI GCATC 3 cut(s) 224, 453, 593
BpiI GAAGAC 1 cut(s) 756
BpmI CTGGAG 1 cut(s) 501
Bpu10I CCTNAGC 1 cut(s) 237
Bpu14I TTCGAA 1 cut(s) 77
BsaAI YACGTR 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 467
BsaJI CCNNGG 3 cut(s) 165, 352, 590
BsaWI WCCGGW 1 cut(s) 547
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse1I ACTGG 2 cut(s) 652, 731
Bse8I GATNNNNATC 1 cut(s) 467
BseBI CCWGG 2 cut(s) 220, 744
BseDI CCNNGG 3 cut(s) 165, 352, 590
BseGI GGATG 2 cut(s) 11, 215
BseJI GATNNNNATC 1 cut(s) 467
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 4 cut(s) 55, 83, 597, 613
BseNI ACTGG 2 cut(s) 652, 731
BseXI GCAGC 6 cut(s) 269, 380, 397, 425, 680, 816
BseYI CCCAGC 1 cut(s) 807
BshFI GGCC 2 cut(s) 63, 540
BsiHKAI GWGCWC 2 cut(s) 600, 620
BsiSI CCGG 2 cut(s) 548, 756
BslI CCNNNNNNNGG 1 cut(s) 547
BsmI GAATGC 3 cut(s) 101, 166, 194
BsnI GGCC 2 cut(s) 63, 540
Bsp119I TTCGAA 1 cut(s) 77
Bsp1286I GDGCHC 2 cut(s) 600, 620
Bsp143I GATC 3 cut(s) 405, 477, 669
Bsp19I CCATGG 1 cut(s) 590
BspACI CCGC 4 cut(s) 290, 431, 453, 606
BspANI GGCC 2 cut(s) 63, 540
BspCNI CTCAG 4 cut(s) 54, 82, 596, 612
BspMAI CTGCAG 1 cut(s) 415
BspPI GGATC 1 cut(s) 677
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 77
BsrI ACTGG 2 cut(s) 652, 731
BssECI CCNNGG 3 cut(s) 165, 352, 590
BssMI GATC 3 cut(s) 405, 477, 669
BssT1I CCWWGG 3 cut(s) 165, 352, 590
Bst2UI CCWGG 2 cut(s) 220, 744
Bst4CI ACNGT 6 cut(s) 188, 389, 424, 536, 705, 769
Bst6I CTCTTC 2 cut(s) 12, 533
BstAPI GCANNNNNTGC 1 cut(s) 463
BstBAI YACGTR 1 cut(s) 114
BstBI TTCGAA 1 cut(s) 77
BstC8I GCNNGC 4 cut(s) 65, 84, 225, 455
BstDEI CTNAG 5 cut(s) 41, 69, 237, 583, 599
BstDSI CCRYGG 1 cut(s) 590
BstF5I GGATG 2 cut(s) 11, 215
BstKTI GATC 3 cut(s) 408, 480, 672
BstMBI GATC 3 cut(s) 405, 477, 669
BstMWI GCNNNNNNNGC 4 cut(s) 374, 407, 463, 595
BstNI CCWGG 2 cut(s) 220, 744
BstNSI RCATGY 2 cut(s) 53, 227
BstSCI CCNGG 2 cut(s) 218, 742
BstSFI CTRYAG 1 cut(s) 411
BstSNI TACGTA 1 cut(s) 114
BstV1I GCAGC 6 cut(s) 269, 380, 397, 425, 680, 816
BstV2I GAAGAC 1 cut(s) 756
BsuRI GGCC 2 cut(s) 63, 540
BtgI CCRYGG 1 cut(s) 590
BtsCI GGATG 2 cut(s) 11, 215
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 5 cut(s) 267, 429, 541, 701, 774
Cac8I GCNNGC 4 cut(s) 65, 84, 225, 455
Cfr13I GGNCC 1 cut(s) 658
Csp6I GTAC 3 cut(s) 47, 312, 651
CviAII CATG 9 cut(s) 50, 156, 224, 287, 301, 473, 591, 632, 848
CviQI GTAC 3 cut(s) 47, 312, 651
DdeI CTNAG 5 cut(s) 41, 69, 237, 583, 599
DpnI GATC 3 cut(s) 407, 479, 671
DpnII GATC 3 cut(s) 405, 477, 669
EaeI YGGCCR 1 cut(s) 538
Eam1104I CTCTTC 2 cut(s) 12, 533
EarI CTCTTC 2 cut(s) 12, 533
Ecl136II GAGCTC 1 cut(s) 598
Eco105I TACGTA 1 cut(s) 114
Eco130I CCWWGG 3 cut(s) 165, 352, 590
Eco24I GRGCYC 1 cut(s) 600
Eco47I GGWCC 1 cut(s) 658
Eco53kI GAGCTC 1 cut(s) 598
EcoICRI GAGCTC 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 73
EcoRII CCWGG 2 cut(s) 218, 742
EcoT14I CCWWGG 3 cut(s) 165, 352, 590
EcoT38I GRGCYC 1 cut(s) 600
ErhI CCWWGG 3 cut(s) 165, 352, 590
FaeI CATG 9 cut(s) 53, 159, 227, 290, 304, 476, 594, 635, 851
FatI CATG 9 cut(s) 49, 155, 223, 286, 300, 472, 590, 631, 847
FauI CCCGC 2 cut(s) 424, 446
FauNDI CATATG 1 cut(s) 459
FbaI TGATCA 1 cut(s) 477
FblI GTMKAC 1 cut(s) 104
Fnu4HI GCNGC 7 cut(s) 258, 291, 369, 411, 414, 694, 805
FokI GGATG 1 cut(s) 202
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 7 cut(s) 258, 291, 369, 411, 414, 694, 805
FspBI CTAG 1 cut(s) 819
GluI GCNGC 7 cut(s) 258, 291, 369, 411, 414, 694, 805
GsaI CCCAGC 1 cut(s) 811
GsuI CTGGAG 1 cut(s) 501
HaeIII GGCC 2 cut(s) 63, 540
HapII CCGG 2 cut(s) 548, 756
Hin1II CATG 9 cut(s) 53, 159, 227, 290, 304, 476, 594, 635, 851
HincII GTYRAC 1 cut(s) 105
HindII GTYRAC 1 cut(s) 105
HinfI GANTC 3 cut(s) 321, 525, 752
HpaII CCGG 2 cut(s) 548, 756
HphI GGTGA 2 cut(s) 155, 758
Hpy166II GTNNAC 2 cut(s) 105, 825
Hpy188I TCNGA 6 cut(s) 16, 44, 72, 586, 602, 615
Hpy188III TCNNGA 2 cut(s) 437, 673
Hpy8I GTNNAC 2 cut(s) 105, 825
HpyCH4III ACNGT 6 cut(s) 188, 389, 424, 536, 705, 769
HpyCH4IV ACGT 2 cut(s) 113, 681
HpyCH4V TGCA 8 cut(s) 99, 215, 227, 300, 368, 393, 413, 722
HpyF10VI GCNNNNNNNGC 4 cut(s) 374, 407, 463, 595
HpyF3I CTNAG 5 cut(s) 41, 69, 237, 583, 599
HpySE526I ACGT 2 cut(s) 113, 681
Hsp92II CATG 9 cut(s) 53, 159, 227, 290, 304, 476, 594, 635, 851
Ksp22I TGATCA 1 cut(s) 477
Kzo9I GATC 3 cut(s) 405, 477, 669
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 595
Lsp1109I GCAGC 6 cut(s) 269, 380, 397, 425, 680, 816
LweI GCATC 3 cut(s) 224, 453, 593
MaeI CTAG 1 cut(s) 819
MaeII ACGT 2 cut(s) 113, 681
MaeIII GTNAC 3 cut(s) 143, 344, 686
MalI GATC 3 cut(s) 407, 479, 671
MboI GATC 3 cut(s) 405, 477, 669
MboII GAAGA 6 cut(s) 29, 475, 501, 520, 688, 761
MfeI CAATTG 1 cut(s) 448
MhlI GDGCHC 2 cut(s) 600, 620
MlsI TGGCCA 1 cut(s) 540
MluCI AATT 5 cut(s) 32, 73, 359, 448, 556
MluNI TGGCCA 1 cut(s) 540
MlyI GAGTC 3 cut(s) 315, 519, 746
MnlI CCTC 4 cut(s) 78, 367, 649, 660
Mox20I TGGCCA 1 cut(s) 540
MscI TGGCCA 1 cut(s) 540
MseI TTAA 3 cut(s) 279, 555, 564
Msp20I TGGCCA 1 cut(s) 540
MspA1I CMGCKG 2 cut(s) 693, 807
MspI CCGG 2 cut(s) 548, 756
MspR9I CCNGG 2 cut(s) 220, 744
MunI CAATTG 1 cut(s) 448
Mva1269I GAATGC 3 cut(s) 101, 166, 194
MvaI CCWGG 2 cut(s) 220, 744
MwoI GCNNNNNNNGC 4 cut(s) 374, 407, 463, 595
NcoI CCATGG 1 cut(s) 590
NdeI CATATG 1 cut(s) 459
NdeII GATC 3 cut(s) 405, 477, 669
NlaIII CATG 9 cut(s) 53, 159, 227, 290, 304, 476, 594, 635, 851
NmuCI GTSAC 2 cut(s) 143, 344
NspI RCATGY 2 cut(s) 53, 227
NspV TTCGAA 1 cut(s) 77
PaeI GCATGC 1 cut(s) 227
PciI ACATGT 1 cut(s) 49
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 3 cut(s) 83, 110, 678
PctI GAATGC 3 cut(s) 101, 166, 194
PkrI GCNGC 7 cut(s) 259, 292, 370, 412, 415, 695, 806
PleI GAGTC 3 cut(s) 315, 519, 746
PpsI GAGTC 3 cut(s) 315, 519, 746
Ppu21I YACGTR 1 cut(s) 114
PscI ACATGT 1 cut(s) 49
PshBI ATTAAT 2 cut(s) 279, 564
Psp124BI GAGCTC 1 cut(s) 600
Psp1406I AACGTT 1 cut(s) 681
Psp6I CCWGG 2 cut(s) 218, 742
PspFI CCCAGC 1 cut(s) 807
PspGI CCWGG 2 cut(s) 218, 742
PspPI GGNCC 1 cut(s) 658
PsrI GAACNNNNNNTAC 2 cut(s) 671, 703
PstI CTGCAG 1 cut(s) 415
PvuII CAGCTG 2 cut(s) 693, 807
RsaI GTAC 3 cut(s) 48, 313, 652
RsaNI GTAC 3 cut(s) 47, 312, 651
SacI GAGCTC 1 cut(s) 600
SalI GTCGAC 1 cut(s) 103
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 279, 555, 564
SatI GCNGC 7 cut(s) 258, 291, 369, 411, 414, 694, 805
Sau3AI GATC 3 cut(s) 405, 477, 669
Sau96I GGNCC 1 cut(s) 658
SchI GAGTC 3 cut(s) 315, 519, 746
ScrFI CCNGG 2 cut(s) 220, 744
SduI GDGCHC 2 cut(s) 600, 620
SfaNI GCATC 3 cut(s) 224, 453, 593
SfcI CTRYAG 1 cut(s) 411
SfuI TTCGAA 1 cut(s) 77
SinI GGWCC 1 cut(s) 658
SnaBI TACGTA 1 cut(s) 114
SphI GCATGC 1 cut(s) 227
Sse9I AATT 5 cut(s) 32, 73, 359, 448, 556
SsiI CCGC 4 cut(s) 290, 431, 453, 606
SspMI CTAG 1 cut(s) 819
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 2 cut(s) 218, 742
StyI CCWWGG 3 cut(s) 165, 352, 590
TaaI ACNGT 6 cut(s) 188, 389, 424, 536, 705, 769
TaiI ACGT 2 cut(s) 116, 684
TaqI TCGA 5 cut(s) 77, 104, 190, 531, 672
TasI AATT 5 cut(s) 32, 73, 359, 448, 556
TatI WGTACW 2 cut(s) 46, 311
TauI GCSGC 1 cut(s) 293
Tru1I TTAA 3 cut(s) 279, 555, 564
Tru9I TTAA 3 cut(s) 279, 555, 564
TscAI CASTG 5 cut(s) 267, 429, 541, 708, 774
TseFI GTSAC 2 cut(s) 143, 344
TseI GCWGC 6 cut(s) 257, 368, 410, 413, 693, 804
Tsp45I GTSAC 2 cut(s) 143, 344
TspDTI ATGAA 3 cut(s) 317, 501, 550
TspRI CASTG 5 cut(s) 267, 429, 541, 708, 774
VpaK11BI GGWCC 1 cut(s) 658
VspI ATTAAT 2 cut(s) 279, 564
XapI RAATTY 3 cut(s) 32, 73, 359
XceI RCATGY 2 cut(s) 53, 227
XmiI GTMKAC 1 cut(s) 104
XspI CTAG 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.