Rh4DG067700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0017282
Physical Location & Seq
Reverse (-)
10114951 .. 10116206
1256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG067700.1

Sequence Viewer

Length: 396 bp
ATGATGCTAATCCATGTGATCATCTTCTCCTTCACCATTTCCATCAGTTCTTCATCTCCAGCAAGACACTCCGACGGTGGCCAACTTCCGGTGCTTAATTTCATTAATGGGTTGCCGAAAAACTCAGAGCCATGGGAGCTCAGATGCGGTAATCAGAGCACTAACGAACACCATGCGTATTTGAAAACTGGTGCAGAGGACCAATGGAGGATCGAGAAGAACGTTGGTTACAGCTGCGATACGCTGTGGGGAAATCAGTTTGCATACTGGAAAGTTTATCAGCCTGGTGAAGACTCCGGCAAAGCCACAGTGTTCTGGTTGGCTAAAGACGATGGTTTTTATCGCAGCTGGGATAAATCTACATGGACAAGAGAGTTTGAATGGGAACATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

131

Amino Acids

15.24

Weight (kDa)

5.49

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 33 - 128 3.1e-09 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017282)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g05643
rosa_chinensis RchiOBHm_Chr4g0396821
rosa_laevigata RLG00000009502
rosa_multiflora Rmu_sc0000487.1_g000053
rosa_roxburghii Rroxscaffold_5G00341660
rosa_rugosa Rorug03G0347500
rosa_samantha Rh4AG072000 Rh4AG072800 Rh4AG074900 Rh4BG069900 Rh4CG078200 Rh4DG067700
rosa_wichuraiana Rw4G005910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 147
AclI AACGTT 1 cut(s) 222
AclWI GGATC 1 cut(s) 218
AcoI YGGCCR 1 cut(s) 79
AfiI CCNNNNNNNGG 1 cut(s) 88
AgsI TTSAA 2 cut(s) 184, 380
AjnI CCWGG 1 cut(s) 283
AluBI AGCT 3 cut(s) 139, 234, 348
AluI AGCT 3 cut(s) 139, 234, 348
Alw21I GWGCWC 2 cut(s) 141, 161
AlwI GGATC 1 cut(s) 218
AoxI GGCC 1 cut(s) 79
ApeKI GCWGC 2 cut(s) 234, 345
AseI ATTAAT 1 cut(s) 105
AspS9I GGNCC 1 cut(s) 199
AsuHPI GGTGA 2 cut(s) 25, 299
AvaII GGWCC 1 cut(s) 199
BalI TGGCCA 1 cut(s) 81
BanII GRGCYC 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 2 cut(s) 141, 161
BbvI GCAGC 2 cut(s) 221, 357
BccI CCATC 2 cut(s) 50, 326
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 1 cut(s) 285
BclI TGATCA 1 cut(s) 18
BisI GCNGC 2 cut(s) 235, 346
BlsI GCNGC 2 cut(s) 236, 347
Bme1390I CCNGG 1 cut(s) 285
Bme18I GGWCC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 199
BmrFI CCNGG 1 cut(s) 285
BmsI GCATC 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 42
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 131
BsaWI WCCGGW 1 cut(s) 88
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse1I ACTGG 2 cut(s) 193, 272
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 131
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMII CTCAG 2 cut(s) 138, 154
BseNI ACTGG 2 cut(s) 193, 272
BseXI GCAGC 2 cut(s) 221, 357
BseYI CCCAGC 1 cut(s) 348
BsgI GTGCAG 1 cut(s) 213
BshFI GGCC 1 cut(s) 81
BsiHKAI GWGCWC 2 cut(s) 141, 161
BsiSI CCGG 2 cut(s) 89, 297
BslI CCNNNNNNNGG 1 cut(s) 88
BsnI GGCC 1 cut(s) 81
Bsp1286I GDGCHC 2 cut(s) 141, 161
Bsp143I GATC 2 cut(s) 18, 210
Bsp19I CCATGG 1 cut(s) 131
BspACI CCGC 1 cut(s) 147
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 2 cut(s) 137, 153
BspPI GGATC 1 cut(s) 218
BsrI ACTGG 2 cut(s) 193, 272
BssECI CCNNGG 1 cut(s) 131
BssMI GATC 2 cut(s) 18, 210
BssT1I CCWWGG 1 cut(s) 131
Bst2UI CCWGG 1 cut(s) 285
Bst4CI ACNGT 2 cut(s) 77, 310
BstDEI CTNAG 2 cut(s) 124, 140
BstDSI CCRYGG 1 cut(s) 131
BstKTI GATC 2 cut(s) 21, 213
BstMBI GATC 2 cut(s) 18, 210
BstMWI GCNNNNNNNGC 1 cut(s) 136
BstNI CCWGG 1 cut(s) 285
BstSCI CCNGG 1 cut(s) 283
BstV1I GCAGC 2 cut(s) 221, 357
BstV2I GAAGAC 1 cut(s) 297
BsuRI GGCC 1 cut(s) 81
BtgI CCRYGG 1 cut(s) 131
BtsIMutI CAGTG 1 cut(s) 315
Cfr13I GGNCC 1 cut(s) 199
CviAII CATG 5 cut(s) 14, 132, 173, 363, 389
CviJI RGCY 8 cut(s) 81, 130, 139, 234, 283, 305, 323, 348
CviKI_1 RGCY 8 cut(s) 81, 130, 139, 234, 283, 305, 323, 348
DdeI CTNAG 2 cut(s) 124, 140
DpnI GATC 2 cut(s) 20, 212
DpnII GATC 2 cut(s) 18, 210
EaeI YGGCCR 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 131
Eco24I GRGCYC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 131
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 131
FaeI CATG 5 cut(s) 17, 135, 176, 366, 392
FaiI YATR 6 cut(s) 15, 133, 174, 265, 364, 390
FatI CATG 5 cut(s) 13, 131, 172, 362, 388
FbaI TGATCA 1 cut(s) 18
Fnu4HI GCNGC 2 cut(s) 235, 346
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 2 cut(s) 235, 346
GluI GCNGC 2 cut(s) 235, 346
GsaI CCCAGC 1 cut(s) 352
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 1 cut(s) 81
HapII CCGG 2 cut(s) 89, 297
Hin1II CATG 5 cut(s) 17, 135, 176, 366, 392
HinfI GANTC 1 cut(s) 293
HpaII CCGG 2 cut(s) 89, 297
HphI GGTGA 2 cut(s) 25, 299
Hpy188I TCNGA 4 cut(s) 73, 127, 143, 156
Hpy188III TCNNGA 1 cut(s) 214
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 2 cut(s) 77, 310
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 2 cut(s) 194, 263
HpyF10VI GCNNNNNNNGC 1 cut(s) 136
HpyF3I CTNAG 2 cut(s) 124, 140
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 5 cut(s) 17, 135, 176, 366, 392
Ksp22I TGATCA 1 cut(s) 18
Kzo9I GATC 2 cut(s) 18, 210
LmnI GCTCC 1 cut(s) 136
LpnPI CCDG 9 cut(s) 72, 102, 174, 253, 270, 297, 301, 310, 334
Lsp1109I GCAGC 2 cut(s) 221, 357
LweI GCATC 1 cut(s) 134
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 227
MalI GATC 2 cut(s) 20, 212
MboI GATC 2 cut(s) 18, 210
MboII GAAGA 4 cut(s) 16, 42, 229, 302
MhlI GDGCHC 2 cut(s) 141, 161
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 1 cut(s) 97
MluNI TGGCCA 1 cut(s) 81
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 2 cut(s) 190, 201
Mox20I TGGCCA 1 cut(s) 81
MscI TGGCCA 1 cut(s) 81
MseI TTAA 2 cut(s) 96, 105
Msp20I TGGCCA 1 cut(s) 81
MspA1I CMGCKG 2 cut(s) 234, 348
MspI CCGG 2 cut(s) 89, 297
MspR9I CCNGG 1 cut(s) 285
MvaI CCWGG 1 cut(s) 285
MwoI GCNNNNNNNGC 1 cut(s) 136
NcoI CCATGG 1 cut(s) 131
NdeII GATC 2 cut(s) 18, 210
NlaIII CATG 5 cut(s) 17, 135, 176, 366, 392
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PkrI GCNGC 2 cut(s) 236, 347
PleI GAGTC 1 cut(s) 287
PpsI GAGTC 1 cut(s) 287
PshBI ATTAAT 1 cut(s) 105
Psp124BI GAGCTC 1 cut(s) 141
Psp1406I AACGTT 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 283
PspFI CCCAGC 1 cut(s) 348
PspGI CCWGG 1 cut(s) 283
PspPI GGNCC 1 cut(s) 199
PsrI GAACNNNNNNTAC 2 cut(s) 212, 244
PvuII CAGCTG 2 cut(s) 234, 348
SacI GAGCTC 1 cut(s) 141
SaqAI TTAA 2 cut(s) 96, 105
SatI GCNGC 2 cut(s) 235, 346
Sau3AI GATC 2 cut(s) 18, 210
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 285
SduI GDGCHC 2 cut(s) 141, 161
SetI ASST 4 cut(s) 141, 225, 236, 350
SfaNI GCATC 1 cut(s) 134
SinI GGWCC 1 cut(s) 199
Sse9I AATT 1 cut(s) 97
SsiI CCGC 1 cut(s) 147
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 1 cut(s) 283
StyI CCWWGG 1 cut(s) 131
TaaI ACNGT 2 cut(s) 77, 310
TaiI ACGT 1 cut(s) 225
TaqI TCGA 1 cut(s) 213
TasI AATT 1 cut(s) 97
Tru1I TTAA 2 cut(s) 96, 105
Tru9I TTAA 2 cut(s) 96, 105
TscAI CASTG 1 cut(s) 315
TseI GCWGC 2 cut(s) 234, 345
TspDTI ATGAA 2 cut(s) 42, 91
TspRI CASTG 1 cut(s) 315
VpaK11BI GGWCC 1 cut(s) 199
VspI ATTAAT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.