RLG00000009740

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0022746
Physical Location & Seq
Reverse (-)
57186960 .. 57188049
1090 bp
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UTR
Exon/CDS
Intron
RLM00000009740

Sequence Viewer

Length: 306 bp
ATGAAGAAGACACTTCAAGCAGATAATTCCTTTTTCTATAATTGTACAATTTTCCTTATGAAGGTCAAATTAAGAGAGAAGAAGCCAGGCATGCTGTCACATGAACTGAAAGAAGGTCTTGGTGTGCCAGATGGTGCTCCTCCGCCATGGCTCATTAACATACAGTATGGCCAACCGTTGTATGGAAACATGTTTGGTGTTCAGCAGCAAGATCAGCCTAATTATGAGAATGAGAAGAATTTCCTCTGTAAGATGCAGGAGATGGGAGGCAAATCCAACAAAAGGACTTCAAGTTTTAGGGTATAA

Protein Analysis

102

Amino Acids

11.64

Weight (kDa)

9.33

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSP PF04046 28 - 55 9.3e-09 PSP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0022746)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0393571
rosa_laevigata RLG00000009740
rosa_samantha Rh4BG044200 Rh4CG051000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 143
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 238
AfaI GTAC 1 cut(s) 46
AfiI CCNNNNNNNGG 3 cut(s) 61, 182, 282
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 2 cut(s) 17, 291
AjnI CCWGG 1 cut(s) 85
Alw21I GWGCWC 1 cut(s) 139
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 1 cut(s) 205
ApoI RAATTY 1 cut(s) 238
Asp700I GAANNNNTTC 1 cut(s) 239
BalI TGGCCA 1 cut(s) 171
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 1 cut(s) 217
BccI CCATC 2 cut(s) 125, 256
BciT130I CCWGG 1 cut(s) 87
BisI GCNGC 1 cut(s) 206
BlsI GCNGC 1 cut(s) 207
Bme1390I CCNGG 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 1 cut(s) 243
BpiI GAAGAC 1 cut(s) 14
BsaJI CCNNGG 1 cut(s) 146
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 182, 282
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 146
BseLI CCNNNNNNNGG 3 cut(s) 61, 182, 282
BseRI GAGGAG 1 cut(s) 129
BseXI GCAGC 1 cut(s) 217
BshFI GGCC 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 139
BslI CCNNNNNNNGG 3 cut(s) 61, 182, 282
BsnI GGCC 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 139
Bsp1407I TGTACA 1 cut(s) 44
Bsp143I GATC 1 cut(s) 211
Bsp19I CCATGG 1 cut(s) 146
BspACI CCGC 1 cut(s) 143
BspANI GGCC 1 cut(s) 171
BsrGI TGTACA 1 cut(s) 44
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 1 cut(s) 211
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 165, 177
BstAUI TGTACA 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 92
BstDSI CCRYGG 1 cut(s) 146
BstENI CCTNNNNNAGG 1 cut(s) 59
BstKTI GATC 1 cut(s) 214
BstMBI GATC 1 cut(s) 211
BstMWI GCNNNNNNNGC 2 cut(s) 91, 214
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 2 cut(s) 94, 193
BstSCI CCNGG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 217
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 1 cut(s) 171
BtgI CCRYGG 1 cut(s) 146
Cac8I GCNNGC 1 cut(s) 92
Csp6I GTAC 1 cut(s) 45
CviAII CATG 4 cut(s) 91, 101, 147, 190
CviJI RGCY 4 cut(s) 85, 151, 171, 217
CviKI_1 RGCY 4 cut(s) 85, 151, 171, 217
CviQI GTAC 1 cut(s) 45
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 169
EciI GGCGGA 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 146
EcoNI CCTNNNNNAGG 1 cut(s) 59
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 146
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 4 cut(s) 94, 104, 150, 193
FalI AAGNNNNNCTT 2 cut(s) 102, 134
FatI CATG 4 cut(s) 90, 100, 146, 189
Fnu4HI GCNGC 1 cut(s) 206
Fsp4HI GCNGC 1 cut(s) 206
GluI GCNGC 1 cut(s) 206
HaeIII GGCC 1 cut(s) 171
Hin1II CATG 4 cut(s) 94, 104, 150, 193
HpyAV CCTTC 2 cut(s) 55, 107
HpyCH4III ACNGT 2 cut(s) 165, 177
HpyCH4V TGCA 1 cut(s) 256
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 214
Hsp92II CATG 4 cut(s) 94, 104, 150, 193
Kzo9I GATC 1 cut(s) 211
LmnI GCTCC 1 cut(s) 142
LpnPI CCDG 4 cut(s) 72, 99, 141, 242
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 1 cut(s) 243
MaeIII GTNAC 1 cut(s) 96
MalI GATC 1 cut(s) 213
MboI GATC 1 cut(s) 211
MboII GAAGA 4 cut(s) 16, 19, 91, 247
MhlI GDGCHC 1 cut(s) 139
MlsI TGGCCA 1 cut(s) 171
MluCI AATT 6 cut(s) 25, 40, 48, 68, 220, 238
MluNI TGGCCA 1 cut(s) 171
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 3 cut(s) 150, 254, 260
Mox20I TGGCCA 1 cut(s) 171
MroXI GAANNNNTTC 1 cut(s) 239
MscI TGGCCA 1 cut(s) 171
MseI TTAA 2 cut(s) 71, 156
Msp20I TGGCCA 1 cut(s) 171
MspR9I CCNGG 1 cut(s) 87
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 91, 214
NcoI CCATGG 1 cut(s) 146
NdeII GATC 1 cut(s) 211
NlaIII CATG 4 cut(s) 94, 104, 150, 193
NmuCI GTSAC 1 cut(s) 96
NspI RCATGY 2 cut(s) 94, 193
PaeI GCATGC 1 cut(s) 94
PciI ACATGT 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 239
PkrI GCNGC 1 cut(s) 207
PscI ACATGT 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 85
PspGI CCWGG 1 cut(s) 85
RsaI GTAC 1 cut(s) 46
RsaNI GTAC 1 cut(s) 45
SaqAI TTAA 2 cut(s) 71, 156
SatI GCNGC 1 cut(s) 206
Sau3AI GATC 1 cut(s) 211
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 139
SetI ASST 2 cut(s) 66, 118
SfaNI GCATC 1 cut(s) 243
SphI GCATGC 1 cut(s) 94
Sse9I AATT 6 cut(s) 25, 40, 48, 68, 220, 238
SsiI CCGC 1 cut(s) 143
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 2 cut(s) 165, 177
TasI AATT 6 cut(s) 25, 40, 48, 68, 220, 238
TatI WGTACW 1 cut(s) 44
Tru1I TTAA 2 cut(s) 71, 156
Tru9I TTAA 2 cut(s) 71, 156
TseFI GTSAC 1 cut(s) 96
TseI GCWGC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 96
TspDTI ATGAA 3 cut(s) 17, 74, 117
XagI CCTNNNNNAGG 1 cut(s) 59
XapI RAATTY 1 cut(s) 238
XceI RCATGY 2 cut(s) 94, 193
XcmI CCANNNNNNNNNTGG 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.