RLG00000010285

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0021451
Physical Location & Seq
Forward (+)
191783 .. 193027
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010285

Sequence Viewer

Length: 675 bp
ATGGAACTCTGTGTCTCTTTGATTCTCTTTCTGATTTCTCTGCCAGTATTTTTGGCCAAAGATGTTGGACATGCTAGCGTTGTAGTTGATGGGACAGTAGCTACTGCACAGAGCTTGTCTCATCCTCTGCTTGCCAAGTCAATTCAAGGTTTAATTGGGGGTTCCTTGCAATATCAAGTATTGTATGGTGTAGGAGATTTGAAATATTCTTGCCATCCTTTCAGAAAGCAGAAGGGTGGGTTGTTTGGATTTTCTACAGGATGTTTACCTATGAGTAGATGGGATGAGCTTAACCATTTTTTCAGAGGCCACAAAGGCGCGGATGTTTGGGTTGGAGATTGGGACTCTAGCAATGCCTATGATTTTATTAATTATACAGTTTCGAAGGGCTACCACATAAATTCATGGGAGTTGGGTAATGAGCTCTGTGGTAATGGTGTTGGAGCTAGAGTTGGAGCTGAACAGTATGGAAAAGACATGATGAAGCTTAAACAAATTGTCAACCAGTTGTACAATGACTCCCACATAAAGCCTGCACTTGTAGGACCTGGAGGATCCTATGATCAAAAGTGGTTTGCCACGTTTCTTCAGGTTTCTGGTTCGGGCTTAGTCAATGTTGTCACTCAGCATCTTTACAATCTGGGTCTAGGTAAGTTTTGCATGTCAAAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko05205 map00531 map01100 map05205
Pfam Domains
Protein Families
Glyco_hydro_79n

Protein Analysis

225

Amino Acids

24.52

Weight (kDa)

8.22

Isoelectric Point (pI)

30.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 45 - 218 4.7e-65 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021451)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0270651
rosa_laevigata RLG00000010285 RLG00000018863 RLG00000022678
rosa_rugosa Rorug05G0315300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 320
AciI CCGC 1 cut(s) 320
AclWI GGATC 2 cut(s) 549, 562
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 400
AcuI CTGAAG 1 cut(s) 572
AfaI GTAC 1 cut(s) 512
AgsI TTSAA 2 cut(s) 146, 202
AjnI CCWGG 1 cut(s) 547
AluBI AGCT 7 cut(s) 101, 114, 289, 424, 446, 458, 487
AluI AGCT 7 cut(s) 101, 114, 289, 424, 446, 458, 487
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 2 cut(s) 19, 123
AlwI GGATC 2 cut(s) 549, 562
AoxI GGCC 2 cut(s) 54, 307
ApoI RAATTY 1 cut(s) 400
AseI ATTAAT 1 cut(s) 369
AspLEI GCGC 1 cut(s) 320
AspS9I GGNCC 1 cut(s) 545
AsuII TTCGAA 1 cut(s) 383
AsuNHI GCTAGC 1 cut(s) 74
AvaII GGWCC 1 cut(s) 545
BalI TGGCCA 1 cut(s) 56
BamHI GGATCC 1 cut(s) 554
BanII GRGCYC 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 426
BccI CCATC 3 cut(s) 83, 222, 273
BciT130I CCWGG 1 cut(s) 549
BclI TGATCA 1 cut(s) 562
BcoDI GTCTC 2 cut(s) 19, 123
BfaI CTAG 4 cut(s) 75, 348, 447, 647
BfmI CTRYAG 1 cut(s) 255
BglI GCCNNNNNGGC 1 cut(s) 315
Bme1390I CCNGG 1 cut(s) 549
Bme18I GGWCC 1 cut(s) 545
BmgT120I GGNCC 1 cut(s) 545
BmiI GGNNCC 2 cut(s) 163, 556
BmrFI CCNGG 1 cut(s) 549
BmsI GCATC 1 cut(s) 637
BmtI GCTAGC 1 cut(s) 78
BplI GAGNNNNNCTC 2 cut(s) 103, 135
BpmI CTGGAG 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 383
BsaXI ACNNNNNCTCC 4 cut(s) 435, 465, 503, 533
Bse1I ACTGG 2 cut(s) 44, 505
Bse3DI GCAATG 1 cut(s) 358
BseBI CCWGG 1 cut(s) 549
BseGI GGATG 5 cut(s) 121, 214, 266, 289, 328
BseMI GCAATG 1 cut(s) 358
BseMII CTCAG 1 cut(s) 638
BseNI ACTGG 2 cut(s) 44, 505
BsgI GTGCAG 2 cut(s) 90, 519
Bsh1236I CGCG 1 cut(s) 320
BshFI GGCC 2 cut(s) 56, 309
BsiHKAI GWGCWC 1 cut(s) 426
BslFI GGGAC 2 cut(s) 106, 356
BsmAI GTCTC 2 cut(s) 19, 123
BsmFI GGGAC 2 cut(s) 106, 356
BsnI GGCC 2 cut(s) 56, 309
Bsp119I TTCGAA 1 cut(s) 383
Bsp1286I GDGCHC 1 cut(s) 426
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 2 cut(s) 554, 562
BspACI CCGC 1 cut(s) 320
BspANI GGCC 2 cut(s) 56, 309
BspCNI CTCAG 1 cut(s) 637
BspFNI CGCG 1 cut(s) 320
BspLI GGNNCC 2 cut(s) 163, 556
BspOI GCTAGC 1 cut(s) 78
BspPI GGATC 2 cut(s) 549, 562
BspT104I TTCGAA 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 358
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 2 cut(s) 44, 505
BssMI GATC 2 cut(s) 554, 562
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 3 cut(s) 97, 379, 465
BstAUI TGTACA 1 cut(s) 510
BstBI TTCGAA 1 cut(s) 383
BstC8I GCNNGC 3 cut(s) 76, 132, 534
BstDEI CTNAG 2 cut(s) 607, 624
BstF5I GGATG 5 cut(s) 121, 214, 266, 289, 328
BstFNI CGCG 1 cut(s) 320
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 2 cut(s) 557, 565
BstMAI GTCTC 2 cut(s) 19, 123
BstMBI GATC 2 cut(s) 554, 562
BstMWI GCNNNNNNNGC 1 cut(s) 315
BstNI CCWGG 1 cut(s) 549
BstNSI RCATGY 2 cut(s) 74, 664
BstSCI CCNGG 1 cut(s) 547
BstSFI CTRYAG 1 cut(s) 255
BstUI CGCG 1 cut(s) 320
BstX2I RGATCY 1 cut(s) 554
BstYI RGATCY 1 cut(s) 554
BsuRI GGCC 2 cut(s) 56, 309
BtsCI GGATG 5 cut(s) 121, 214, 266, 289, 328
Cac8I GCNNGC 3 cut(s) 76, 132, 534
CfoI GCGC 1 cut(s) 320
Cfr13I GGNCC 1 cut(s) 545
Csp6I GTAC 1 cut(s) 511
CviAII CATG 4 cut(s) 71, 405, 478, 661
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 2 cut(s) 607, 624
DpnI GATC 2 cut(s) 556, 564
DpnII GATC 2 cut(s) 554, 562
EaeI YGGCCR 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 426
Eco47I GGWCC 1 cut(s) 545
Eco53kI GAGCTC 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 572
EcoICRI GAGCTC 1 cut(s) 424
EcoO109I RGGNCCY 1 cut(s) 545
EcoRII CCWGG 1 cut(s) 547
EcoT38I GRGCYC 1 cut(s) 426
FaeI CATG 4 cut(s) 74, 408, 481, 664
FaqI GGGAC 2 cut(s) 106, 356
FatI CATG 4 cut(s) 70, 404, 477, 660
FbaI TGATCA 1 cut(s) 562
FokI GGATG 5 cut(s) 108, 201, 273, 296, 335
FriOI GRGCYC 1 cut(s) 426
FspBI CTAG 4 cut(s) 75, 348, 447, 647
GlaI GCGC 1 cut(s) 319
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 2 cut(s) 56, 309
HhaI GCGC 1 cut(s) 320
Hin1II CATG 4 cut(s) 74, 408, 481, 664
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 3 cut(s) 22, 344, 518
Hpy166II GTNNAC 2 cut(s) 266, 502
Hpy188I TCNGA 3 cut(s) 33, 224, 305
Hpy8I GTNNAC 2 cut(s) 266, 502
HpyAV CCTTC 2 cut(s) 226, 379
HpyCH4III ACNGT 3 cut(s) 97, 379, 465
HpyCH4IV ACGT 1 cut(s) 581
HpyCH4V TGCA 4 cut(s) 107, 169, 536, 660
HpyF10VI GCNNNNNNNGC 1 cut(s) 315
HpyF3I CTNAG 2 cut(s) 607, 624
HpySE526I ACGT 1 cut(s) 581
Hsp92II CATG 4 cut(s) 74, 408, 481, 664
HspAI GCGC 1 cut(s) 318
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 2 cut(s) 554, 562
LmnI GCTCC 2 cut(s) 443, 455
LpnPI CCDG 9 cut(s) 57, 243, 518, 534, 546, 561, 575, 582, 626
LweI GCATC 1 cut(s) 637
MaeI CTAG 4 cut(s) 75, 348, 447, 647
MaeII ACGT 1 cut(s) 581
MaeIII GTNAC 1 cut(s) 619
MalI GATC 2 cut(s) 556, 564
MboI GATC 2 cut(s) 554, 562
MboII GAAGA 1 cut(s) 578
MflI RGATCY 1 cut(s) 554
MhlI GDGCHC 1 cut(s) 426
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 5 cut(s) 141, 153, 370, 400, 495
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 2 cut(s) 338, 512
MmeI TCCRAC 4 cut(s) 46, 313, 421, 433
MnlI CCTC 3 cut(s) 135, 299, 545
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MseI TTAA 4 cut(s) 152, 291, 369, 489
Msp20I TGGCCA 1 cut(s) 56
MspR9I CCNGG 1 cut(s) 549
MvaI CCWGG 1 cut(s) 549
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 1 cut(s) 315
NdeII GATC 2 cut(s) 554, 562
NheI GCTAGC 1 cut(s) 74
NlaIII CATG 4 cut(s) 74, 408, 481, 664
NlaIV GGNNCC 2 cut(s) 163, 556
NmuCI GTSAC 1 cut(s) 619
NspI RCATGY 2 cut(s) 74, 664
NspV TTCGAA 1 cut(s) 383
PfeI GAWTC 1 cut(s) 22
PleI GAGTC 2 cut(s) 338, 512
PpsI GAGTC 2 cut(s) 338, 512
PpuMI RGGWCCY 1 cut(s) 545
PshBI ATTAAT 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 426
Psp5II RGGWCCY 1 cut(s) 545
Psp6I CCWGG 1 cut(s) 547
PspGI CCWGG 1 cut(s) 547
PspN4I GGNNCC 2 cut(s) 163, 556
PspPI GGNCC 1 cut(s) 545
PspPPI RGGWCCY 1 cut(s) 545
PsuI RGATCY 1 cut(s) 554
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
SacI GAGCTC 1 cut(s) 426
SaqAI TTAA 4 cut(s) 152, 291, 369, 489
Sau3AI GATC 2 cut(s) 554, 562
Sau96I GGNCC 1 cut(s) 545
SchI GAGTC 2 cut(s) 338, 512
ScrFI CCNGG 1 cut(s) 549
SduI GDGCHC 1 cut(s) 426
SfaNI GCATC 1 cut(s) 637
SfcI CTRYAG 1 cut(s) 255
SfuI TTCGAA 1 cut(s) 383
SinI GGWCC 1 cut(s) 545
Sse9I AATT 5 cut(s) 141, 153, 370, 400, 495
SsiI CCGC 1 cut(s) 320
SspI AATATT 1 cut(s) 206
SspMI CTAG 4 cut(s) 75, 348, 447, 647
SstI GAGCTC 1 cut(s) 426
StyD4I CCNGG 1 cut(s) 547
TaaI ACNGT 3 cut(s) 97, 379, 465
TaiI ACGT 1 cut(s) 584
TaqI TCGA 1 cut(s) 383
TasI AATT 5 cut(s) 141, 153, 370, 400, 495
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 4 cut(s) 152, 291, 369, 489
Tru9I TTAA 4 cut(s) 152, 291, 369, 489
TseFI GTSAC 1 cut(s) 619
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 2 cut(s) 393, 497
VpaK11BI GGWCC 1 cut(s) 545
VspI ATTAAT 1 cut(s) 369
XapI RAATTY 1 cut(s) 400
XceI RCATGY 2 cut(s) 74, 664
XspI CTAG 4 cut(s) 75, 348, 447, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.