RLG00000018863

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0021451
Physical Location & Seq
Forward (+)
38221356 .. 38222205
850 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018863

Sequence Viewer

Length: 540 bp
ATGCCCATAGAGGTAGAGGTAGCGGAAGTGGCTCAGCCATTAGGAACCTGTTTTCGGATAACAAGTAGGGTTTGCAATAACACCCCTTCCAACAGTATAACTAGAGCCTCTGGAGCTATCACCAGCGCACTGTCAGAGAGGCACCACAAAGGCGCGGATGTTTGGGTTGGAAATTGGGACTCTAGCAATGCCTATGATTTTATTAATTATACAGTTTCGAAGGGCTACCACATAAATTCATGGGAGTTGGCTGAACAGTATGGAAAAGACATGATGAAGCTTAAACAAATTGTCAACCAGTTGTACAATGACTCCCACATAAAGCCTGCAATTGTAGGACCTGGAGGATTCTATGATCAAAAGTGGTTTGCCACGCTTCTTCAGGTTTCTGGTTCAGGTGTAGTCAATGTTGTCACTCAGCATCTTTACAATTTGGGTCCTGGTCATATTGAACTATTGGCTTCAGGAACCCTTCCTCTGTTTTACCTAACAGTCTCCAAAGAAGATAATAAAAGGGAAATCCTCTTTATGTACTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko05205 map00531 map01100 map05205
Pfam Domains
Protein Families
Glyco_hydro_79n

Protein Analysis

180

Amino Acids

20.03

Weight (kDa)

6.28

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 42 - 164 1.3e-36 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021451)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0270651
rosa_laevigata RLG00000010285 RLG00000018863 RLG00000022678
rosa_rugosa Rorug05G0315300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 141
AccII CGCG 1 cut(s) 155
AciI CCGC 2 cut(s) 23, 155
AcsI RAATTY 1 cut(s) 235
AcuI CTGAAG 2 cut(s) 365, 447
AfaI GTAC 2 cut(s) 305, 533
AfiI CCNNNNNNNGG 1 cut(s) 54
AgsI TTSAA 1 cut(s) 452
AjnI CCWGG 2 cut(s) 340, 439
AluBI AGCT 2 cut(s) 116, 280
AluI AGCT 2 cut(s) 116, 280
Alw26I GTCTC 1 cut(s) 499
ApoI RAATTY 1 cut(s) 235
AseI ATTAAT 1 cut(s) 204
AspLEI GCGC 2 cut(s) 128, 155
AspS9I GGNCC 2 cut(s) 338, 437
AsuHPI GGTGA 1 cut(s) 112
AsuII TTCGAA 1 cut(s) 218
AvaII GGWCC 2 cut(s) 338, 437
BanI GGYRCC 1 cut(s) 141
BciT130I CCWGG 2 cut(s) 342, 441
BclI TGATCA 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 499
BfaI CTAG 2 cut(s) 102, 183
BlpI GCTNAGC 1 cut(s) 33
Bme1390I CCNGG 2 cut(s) 342, 441
Bme18I GGWCC 2 cut(s) 338, 437
BmgT120I GGNCC 2 cut(s) 338, 437
BmiI GGNNCC 4 cut(s) 46, 143, 438, 469
BmrFI CCNGG 2 cut(s) 342, 441
BmsI GCATC 1 cut(s) 430
BpmI CTGGAG 2 cut(s) 132, 363
Bpu1102I GCTNAGC 1 cut(s) 33
Bpu14I TTCGAA 1 cut(s) 218
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 1 cut(s) 54
Bse1I ACTGG 2 cut(s) 298, 539
Bse3DI GCAATG 1 cut(s) 193
BseBI CCWGG 2 cut(s) 342, 441
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 1 cut(s) 54
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 2 cut(s) 47, 431
BseNI ACTGG 2 cut(s) 298, 539
Bsh1236I CGCG 1 cut(s) 155
BshNI GGYRCC 1 cut(s) 141
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 54
BsmAI GTCTC 1 cut(s) 499
BsmFI GGGAC 1 cut(s) 191
Bsp119I TTCGAA 1 cut(s) 218
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 1 cut(s) 355
Bsp1720I GCTNAGC 1 cut(s) 33
BspACI CCGC 2 cut(s) 23, 155
BspCNI CTCAG 2 cut(s) 46, 430
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 4 cut(s) 46, 143, 438, 469
BspT104I TTCGAA 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 193
BsrGI TGTACA 1 cut(s) 303
BsrI ACTGG 2 cut(s) 298, 539
BssMI GATC 1 cut(s) 355
Bst2UI CCWGG 2 cut(s) 342, 441
Bst4CI ACNGT 5 cut(s) 95, 132, 214, 258, 493
BstAUI TGTACA 1 cut(s) 303
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 327
BstDEI CTNAG 2 cut(s) 33, 417
BstF5I GGATG 1 cut(s) 163
BstFNI CGCG 1 cut(s) 155
BstHHI GCGC 2 cut(s) 128, 155
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 1 cut(s) 499
BstMBI GATC 1 cut(s) 355
BstMWI GCNNNNNNNGC 2 cut(s) 29, 113
BstNI CCWGG 2 cut(s) 342, 441
BstSCI CCNGG 2 cut(s) 340, 439
BstUI CGCG 1 cut(s) 155
BtsCI GGATG 1 cut(s) 163
BtsIMutI CAGTG 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 327
CfoI GCGC 2 cut(s) 128, 155
Cfr13I GGNCC 2 cut(s) 338, 437
Csp6I GTAC 2 cut(s) 304, 532
CviAII CATG 2 cut(s) 240, 271
CviJI RGCY 9 cut(s) 32, 37, 107, 116, 225, 251, 280, 325, 461
CviKI_1 RGCY 9 cut(s) 32, 37, 107, 116, 225, 251, 280, 325, 461
CviQI GTAC 2 cut(s) 304, 532
DdeI CTNAG 2 cut(s) 33, 417
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
Eco47I GGWCC 2 cut(s) 338, 437
Eco57I CTGAAG 2 cut(s) 365, 447
EcoO109I RGGNCCY 2 cut(s) 338, 437
EcoRII CCWGG 2 cut(s) 340, 439
FaeI CATG 2 cut(s) 243, 274
FaqI GGGAC 1 cut(s) 191
FatI CATG 2 cut(s) 239, 270
FbaI TGATCA 1 cut(s) 355
FokI GGATG 1 cut(s) 170
FspBI CTAG 2 cut(s) 102, 183
GlaI GCGC 2 cut(s) 127, 154
GsuI CTGGAG 2 cut(s) 132, 363
HhaI GCGC 2 cut(s) 128, 155
Hin1II CATG 2 cut(s) 243, 274
Hin6I GCGC 2 cut(s) 126, 153
HinP1I GCGC 2 cut(s) 126, 153
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 3 cut(s) 179, 311, 348
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 2 cut(s) 57, 136
Hpy188III TCNNGA 2 cut(s) 111, 465
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 3 cut(s) 96, 214, 482
HpyCH4III ACNGT 5 cut(s) 95, 132, 214, 258, 493
HpyCH4V TGCA 2 cut(s) 75, 329
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 113
HpyF3I CTNAG 2 cut(s) 33, 417
Hsp92II CATG 2 cut(s) 243, 274
HspAI GCGC 2 cut(s) 126, 153
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 1 cut(s) 355
LmnI GCTCC 1 cut(s) 113
LweI GCATC 1 cut(s) 430
MaeI CTAG 2 cut(s) 102, 183
MaeIII GTNAC 1 cut(s) 412
MalI GATC 1 cut(s) 357
MboI GATC 1 cut(s) 355
MboII GAAGA 2 cut(s) 371, 515
MfeI CAATTG 1 cut(s) 330
MluCI AATT 6 cut(s) 172, 205, 235, 288, 330, 430
MlyI GAGTC 2 cut(s) 173, 305
MmeI TCCRAC 2 cut(s) 114, 148
MnlI CCTC 7 cut(s) 4, 10, 118, 132, 338, 486, 533
MseI TTAA 2 cut(s) 204, 282
MspR9I CCNGG 2 cut(s) 342, 441
MunI CAATTG 1 cut(s) 330
MvaI CCWGG 2 cut(s) 342, 441
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 2 cut(s) 29, 113
NdeII GATC 1 cut(s) 355
NlaIII CATG 2 cut(s) 243, 274
NlaIV GGNNCC 4 cut(s) 46, 143, 438, 469
NmuCI GTSAC 1 cut(s) 412
NspV TTCGAA 1 cut(s) 218
PfeI GAWTC 1 cut(s) 348
PleI GAGTC 2 cut(s) 173, 305
PpsI GAGTC 2 cut(s) 173, 305
PpuMI RGGWCCY 2 cut(s) 338, 437
PshBI ATTAAT 1 cut(s) 204
Psp5II RGGWCCY 2 cut(s) 338, 437
Psp6I CCWGG 2 cut(s) 340, 439
PspGI CCWGG 2 cut(s) 340, 439
PspN4I GGNNCC 4 cut(s) 46, 143, 438, 469
PspPI GGNCC 2 cut(s) 338, 437
PspPPI RGGWCCY 2 cut(s) 338, 437
RsaI GTAC 2 cut(s) 305, 533
RsaNI GTAC 2 cut(s) 304, 532
SaqAI TTAA 2 cut(s) 204, 282
Sau3AI GATC 1 cut(s) 355
Sau96I GGNCC 2 cut(s) 338, 437
SchI GAGTC 2 cut(s) 173, 305
ScrFI CCNGG 2 cut(s) 342, 441
SetI ASST 9 cut(s) 15, 21, 50, 118, 282, 343, 387, 400, 489
SfaNI GCATC 1 cut(s) 430
SfuI TTCGAA 1 cut(s) 218
SinI GGWCC 2 cut(s) 338, 437
Sse9I AATT 6 cut(s) 172, 205, 235, 288, 330, 430
SsiI CCGC 2 cut(s) 23, 155
SspMI CTAG 2 cut(s) 102, 183
StyD4I CCNGG 2 cut(s) 340, 439
TaaI ACNGT 5 cut(s) 95, 132, 214, 258, 493
TaqI TCGA 1 cut(s) 218
TasI AATT 6 cut(s) 172, 205, 235, 288, 330, 430
TatI WGTACW 2 cut(s) 303, 531
TfiI GAWTC 1 cut(s) 348
Tru1I TTAA 2 cut(s) 204, 282
Tru9I TTAA 2 cut(s) 204, 282
TscAI CASTG 1 cut(s) 135
TseFI GTSAC 1 cut(s) 412
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 2 cut(s) 228, 290
TspRI CASTG 1 cut(s) 135
VpaK11BI GGWCC 2 cut(s) 338, 437
VspI ATTAAT 1 cut(s) 204
XapI RAATTY 1 cut(s) 235
XspI CTAG 2 cut(s) 102, 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.