RLG00000012546

TENA/THI-4/PQQC family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016260
Physical Location & Seq
Reverse (-)
19758006 .. 19826997
68992 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012546

Sequence Viewer

Length: 1230 bp
ATGAGACTCTATGCGTATATTAGCAATGAAATTCAGGGTATTCTGGATCCTGATGATGATAGTCACATCTATAAAAGGTGGATAGACAGTTTCTCGTCTAAAGATTTTGAGGCAACAGCTCTACAAAATGAAGAGATGCTAGATAAACTTAGTGTCTCTTTGACTGGAGAGGAGCTTGAGATTTTAGAGAAGCTATATCATCAAGCTATGAAACTTGAAGTAGATTTCATCATTAATAGTGGACTGCCAATTCCTCAGAAAGATGTAGTCCCTTTGTCTCGGGTGCATGACTTTACCGACTGCCATCTTACCATACTTTGTGACTTTGACTTGGCATGCACTACTTTCGATTCCTCTGCCATATTAGCTGAGATTGCCATCAGAACAGTACCAAAGACTGAAGCTTATGGATCAGAAACCCAACTTGTCCGGTTGTCTTCAGCAGACTTAAGGAGCACATGGGATATTCTTTCCACCCAATATACAGAAGAGTTTGAACAATGTGTAGAAACCATCGTGACCAACGAGAAAGTGGAAGGATTCCATTATGAAGATCTATGCAGTGCCCTTGAACAACTTGCGGAGTTTGAGAGACAGGCAAATGAAAGGGTGGTTCGGTCAGAAGTTCTAAAGGGTTTAAATCTAGAGGATATAAAACGGGCTGGCCAGGCTCTTATTCTTCAGGATGGTTGTAGAAGCTTTTTTCAGAAGATTGTGAAATATGAAAACCTTAAAACCGATGTTCATGTTCTTTCATACTGCTGGTGTAGTGACCTCATTGTATCAGCTTTTTCTTCAGGAGATCTAAATGCCTTGAGTGTACACTCGAATGAATTGGCTTATGATGAATCAGTTACCACTGGGGAAATTATTAAGAAGTTGGAGTCTCCAATGGAAAAGCTTCAAGCTTTCACTGGCATCCTAAAGGGTCCCTGCAGGAGTAGTGAAGACGAGCACAAGCAATTGACAGTTTACATTGGAGGATCAGTTGGTGACTTTCTTTGCCTCCTTCAAGCAGATATTGGTATTGTAATCGGCTCAAGTTCATGCCTAAGGAGACTAGGTGATCATTTTGGCGTTTCTTTTGTCCCATTGTTCTCTGGCTTGGTGAAGAGGCAGAGACAACTTGCTGCTGATAAAGAAGGTTCCTCTCAATGGAAACCACTGTCTGGCACTCTGTATACAGTCTCTAGCTGGGCCGAAATACATGCATTCATTTTAGGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TENA_THI-4

Protein Analysis

410

Amino Acids

45.83

Weight (kDa)

4.74

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016260)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20480
malus_domestica MD05G1188600.v1.1
prunus_persica Prupe.8G215100_v2.0.a1 Prupe.8G215100_v2.0.a1
pyrus_communis pycom05g17320
rosa_chinensis RchiOBHm_Chr6g0286581
rosa_laevigata RLG00000012546
rosa_multiflora Rmu_sc0000560.1_g000002
rosa_roxburghii Rroxscaffold_7G00182170
rosa_rugosa Rorug06G0181200
rosa_samantha Rh6AG294100 Rh6BG297200 Rh6CG297600 Rh6DG289500
rosa_wichuraiana Rw6G025310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1169
AccI GTMKAC 1 cut(s) 1181
AciI CCGC 1 cut(s) 581
AclWI GGATC 4 cut(s) 41, 54, 418, 991
AcoI YGGCCR 1 cut(s) 664
AcsI RAATTY 1 cut(s) 30
AcuI CTGAAG 4 cut(s) 420, 423, 665, 780
AfaI GTAC 3 cut(s) 390, 822, 1225
AfiI CCNNNNNNNGG 2 cut(s) 1155, 1169
AflII CTTAAG 1 cut(s) 448
AgsI TTSAA 5 cut(s) 218, 497, 572, 905, 1013
AjnI CCWGG 1 cut(s) 666
Alw21I GWGCWC 2 cut(s) 458, 957
Alw26I GTCTC 7 cut(s) 160, 282, 586, 891, 1051, 1114, 1192
AlwI GGATC 4 cut(s) 41, 54, 418, 991
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 2 cut(s) 664, 1197
ApeKI GCWGC 1 cut(s) 1130
ApoI RAATTY 1 cut(s) 30
AseI ATTAAT 1 cut(s) 234
Asp700I GAANNNNTTC 1 cut(s) 900
AspS9I GGNCC 2 cut(s) 929, 1197
AsuHPI GGTGA 3 cut(s) 1004, 1076, 1120
AvaI CYCGRG 1 cut(s) 279
AvaII GGWCC 1 cut(s) 929
AxyI CCTNAGG 1 cut(s) 1052
BaeGI GKGCMC 1 cut(s) 568
BalI TGGCCA 1 cut(s) 666
BamHI GGATCC 1 cut(s) 46
BbsI GAAGAC 2 cut(s) 429, 954
Bbv12I GWGCWC 2 cut(s) 458, 957
BbvI GCAGC 1 cut(s) 1117
BccI CCATC 4 cut(s) 312, 386, 521, 680
BcgI CGANNNNNNTGC 6 cut(s) 328, 338, 362, 372, 1190, 1224
BciT130I CCWGG 1 cut(s) 668
BclI TGATCA 1 cut(s) 1066
BcoDI GTCTC 7 cut(s) 160, 282, 586, 891, 1051, 1114, 1192
BfaI CTAG 4 cut(s) 140, 644, 1061, 1191
BfmI CTRYAG 1 cut(s) 934
BfrI CTTAAG 1 cut(s) 448
BglII AGATCT 2 cut(s) 553, 802
BisI GCNGC 1 cut(s) 1131
BlsI GCNGC 1 cut(s) 1132
Bme1390I CCNGG 1 cut(s) 668
Bme18I GGWCC 1 cut(s) 929
BmeT110I CYCGRG 1 cut(s) 279
BmgT120I GGNCC 2 cut(s) 929, 1197
BmiI GGNNCC 4 cut(s) 48, 930, 931, 1147
BmrFI CCNGG 1 cut(s) 668
BmrI ACTGGG 1 cut(s) 870
BmsI GCATC 2 cut(s) 126, 927
BmuI ACTGGG 1 cut(s) 870
BpiI GAAGAC 2 cut(s) 429, 954
BpmI CTGGAG 1 cut(s) 186
BpuEI CTTGAG 3 cut(s) 197, 835, 1024
BsaBI GATNNNNATC 1 cut(s) 377
BsaWI WCCGGW 1 cut(s) 429
BsaXI ACNNNNNCTCC 4 cut(s) 972, 1002, 1048, 1078
Bsc4I CCNNNNNNNGG 2 cut(s) 1155, 1169
Bse1I ACTGG 3 cut(s) 169, 865, 919
Bse21I CCTNAGG 1 cut(s) 1052
Bse3DI GCAATG 1 cut(s) 31
Bse8I GATNNNNATC 1 cut(s) 377
BseBI CCWGG 1 cut(s) 668
BseGI GGATG 2 cut(s) 691, 918
BseJI GATNNNNATC 1 cut(s) 377
BseLI CCNNNNNNNGG 2 cut(s) 1155, 1169
BseMI GCAATG 1 cut(s) 31
BseMII CTCAG 2 cut(s) 269, 360
BseNI ACTGG 3 cut(s) 169, 865, 919
BseRI GAGGAG 1 cut(s) 185
BseSI GKGCMC 1 cut(s) 568
BseXI GCAGC 1 cut(s) 1117
BseYI CCCAGC 1 cut(s) 1194
BshFI GGCC 2 cut(s) 666, 1199
BsiHKAI GWGCWC 2 cut(s) 458, 957
BsiHKCI CYCGRG 1 cut(s) 279
BsiSI CCGG 1 cut(s) 430
BslFI GGGAC 3 cut(s) 254, 915, 1073
BslI CCNNNNNNNGG 2 cut(s) 1155, 1169
BsmAI GTCTC 7 cut(s) 160, 282, 586, 891, 1051, 1114, 1192
BsmFI GGGAC 3 cut(s) 254, 915, 1073
BsmI GAATGC 1 cut(s) 1211
BsnI GGCC 2 cut(s) 666, 1199
BsoBI CYCGRG 1 cut(s) 279
Bsp1286I GDGCHC 3 cut(s) 458, 568, 957
Bsp1407I TGTACA 1 cut(s) 820
Bsp143I GATC 6 cut(s) 46, 410, 553, 802, 983, 1066
BspACI CCGC 1 cut(s) 581
BspANI GGCC 2 cut(s) 666, 1199
BspCNI CTCAG 2 cut(s) 268, 361
BspLI GGNNCC 4 cut(s) 48, 930, 931, 1147
BspMAI CTGCAG 1 cut(s) 938
BspPI GGATC 4 cut(s) 41, 54, 418, 991
BspTI CTTAAG 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 31
BsrGI TGTACA 1 cut(s) 820
BsrI ACTGG 3 cut(s) 169, 865, 919
BssMI GATC 6 cut(s) 46, 410, 553, 802, 983, 1066
BssNAI GTATAC 1 cut(s) 1182
Bst1107I GTATAC 1 cut(s) 1182
Bst2UI CCWGG 1 cut(s) 668
Bst4CI ACNGT 5 cut(s) 89, 388, 970, 1167, 1186
Bst6I CTCTTC 3 cut(s) 126, 483, 1106
BstAFI CTTAAG 1 cut(s) 448
BstAUI TGTACA 1 cut(s) 820
BstC8I GCNNGC 2 cut(s) 337, 664
BstDEI CTNAG 4 cut(s) 149, 255, 369, 1052
BstF5I GGATG 2 cut(s) 691, 918
BstKTI GATC 6 cut(s) 49, 413, 556, 805, 986, 1069
BstMAI GTCTC 7 cut(s) 160, 282, 586, 891, 1051, 1114, 1192
BstMBI GATC 6 cut(s) 46, 410, 553, 802, 983, 1066
BstMWI GCNNNNNNNGC 3 cut(s) 365, 374, 668
BstNI CCWGG 1 cut(s) 668
BstNSI RCATGY 2 cut(s) 339, 1211
BstSCI CCNGG 1 cut(s) 666
BstSFI CTRYAG 1 cut(s) 934
BstSLI GKGCMC 1 cut(s) 568
BstV1I GCAGC 1 cut(s) 1117
BstV2I GAAGAC 2 cut(s) 429, 954
BstX2I RGATCY 3 cut(s) 46, 553, 802
BstYI RGATCY 3 cut(s) 46, 553, 802
BstZ17I GTATAC 1 cut(s) 1182
Bsu36I CCTNAGG 1 cut(s) 1052
BsuRI GGCC 2 cut(s) 666, 1199
BtsCI GGATG 2 cut(s) 691, 918
BtsI GCAGTG 1 cut(s) 568
BtsIMutI CAGTG 4 cut(s) 568, 858, 912, 1163
Cac8I GCNNGC 2 cut(s) 337, 664
Cfr13I GGNCC 2 cut(s) 929, 1197
Csp6I GTAC 3 cut(s) 389, 821, 1224
CviAII CATG 6 cut(s) 287, 336, 459, 746, 1047, 1208
CviQI GTAC 3 cut(s) 389, 821, 1224
DdeI CTNAG 4 cut(s) 149, 255, 369, 1052
DpnI GATC 6 cut(s) 48, 412, 555, 804, 985, 1068
DpnII GATC 6 cut(s) 46, 410, 553, 802, 983, 1066
DraI TTTAAA 1 cut(s) 639
EaeI YGGCCR 1 cut(s) 664
Eam1104I CTCTTC 3 cut(s) 126, 483, 1106
EarI CTCTTC 3 cut(s) 126, 483, 1106
Eco47I GGWCC 1 cut(s) 929
Eco57I CTGAAG 4 cut(s) 420, 423, 665, 780
Eco81I CCTNAGG 1 cut(s) 1052
Eco88I CYCGRG 1 cut(s) 279
EcoO109I RGGNCCY 1 cut(s) 929
EcoRII CCWGG 1 cut(s) 666
EcoT22I ATGCAT 1 cut(s) 1213
FaeI CATG 6 cut(s) 290, 339, 462, 749, 1050, 1211
FaqI GGGAC 3 cut(s) 254, 915, 1073
FatI CATG 6 cut(s) 286, 335, 458, 745, 1046, 1207
FbaI TGATCA 1 cut(s) 1066
FblI GTMKAC 1 cut(s) 1181
Fnu4HI GCNGC 1 cut(s) 1131
FokI GGATG 2 cut(s) 698, 905
Fsp4HI GCNGC 1 cut(s) 1131
FspBI CTAG 4 cut(s) 140, 644, 1061, 1191
GluI GCNGC 1 cut(s) 1131
GsaI CCCAGC 1 cut(s) 1198
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 2 cut(s) 666, 1199
HapII CCGG 1 cut(s) 430
Hin1II CATG 6 cut(s) 290, 339, 462, 749, 1050, 1211
HindIII AAGCTT 4 cut(s) 402, 697, 899, 906
HinfI GANTC 5 cut(s) 6, 350, 540, 848, 884
HpaII CCGG 1 cut(s) 430
HphI GGTGA 3 cut(s) 1004, 1076, 1120
Hpy166II GTNNAC 5 cut(s) 242, 821, 823, 973, 1182
Hpy188I TCNGA 5 cut(s) 258, 383, 415, 622, 708
Hpy188III TCNNGA 6 cut(s) 44, 50, 517, 644, 683, 798
Hpy8I GTNNAC 5 cut(s) 242, 821, 823, 973, 1182
HpyAV CCTTC 3 cut(s) 530, 1019, 1136
HpyCH4III ACNGT 5 cut(s) 89, 388, 970, 1167, 1186
HpyCH4V TGCA 5 cut(s) 286, 339, 561, 936, 1211
HpyF10VI GCNNNNNNNGC 3 cut(s) 365, 374, 668
HpyF3I CTNAG 4 cut(s) 149, 255, 369, 1052
Hsp92II CATG 6 cut(s) 290, 339, 462, 749, 1050, 1211
KflI GGGWCCC 1 cut(s) 929
Ksp22I TGATCA 1 cut(s) 1066
Kzo9I GATC 6 cut(s) 46, 410, 553, 802, 983, 1066
LmnI GCTCC 2 cut(s) 172, 453
Lsp1109I GCAGC 1 cut(s) 1117
LweI GCATC 2 cut(s) 126, 927
MaeI CTAG 4 cut(s) 140, 644, 1061, 1191
MaeIII GTNAC 6 cut(s) 62, 320, 517, 770, 853, 992
MalI GATC 6 cut(s) 48, 412, 555, 804, 985, 1068
MboI GATC 6 cut(s) 46, 410, 553, 802, 983, 1066
MboII GAAGA 9 cut(s) 143, 429, 500, 563, 671, 721, 786, 959, 1123
MfeI CAATTG 1 cut(s) 962
MflI RGATCY 3 cut(s) 46, 553, 802
MhlI GDGCHC 3 cut(s) 458, 568, 957
MlsI TGGCCA 1 cut(s) 666
MluCI AATT 5 cut(s) 30, 249, 833, 867, 962
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 1 cut(s) 893
MmeI TCCRAC 1 cut(s) 861
Mox20I TGGCCA 1 cut(s) 666
Mph1103I ATGCAT 1 cut(s) 1213
MroXI GAANNNNTTC 1 cut(s) 900
MscI TGGCCA 1 cut(s) 666
MseI TTAA 5 cut(s) 234, 449, 638, 732, 873
MslI CAYNNNNRTG 1 cut(s) 828
Msp20I TGGCCA 1 cut(s) 666
MspCI CTTAAG 1 cut(s) 448
MspI CCGG 1 cut(s) 430
MspR9I CCNGG 1 cut(s) 668
MunI CAATTG 1 cut(s) 962
Mva1269I GAATGC 1 cut(s) 1211
MvaI CCWGG 1 cut(s) 668
MwoI GCNNNNNNNGC 3 cut(s) 365, 374, 668
NdeII GATC 6 cut(s) 46, 410, 553, 802, 983, 1066
NlaIII CATG 6 cut(s) 290, 339, 462, 749, 1050, 1211
NlaIV GGNNCC 4 cut(s) 48, 930, 931, 1147
NmuCI GTSAC 5 cut(s) 62, 320, 517, 770, 992
NsiI ATGCAT 1 cut(s) 1213
NspI RCATGY 2 cut(s) 339, 1211
PaeI GCATGC 1 cut(s) 339
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PctI GAATGC 1 cut(s) 1211
PdmI GAANNNNTTC 1 cut(s) 900
PfeI GAWTC 3 cut(s) 350, 540, 848
PflMI CCANNNNNTGG 1 cut(s) 1169
PkrI GCNGC 1 cut(s) 1132
PleI GAGTC 1 cut(s) 892
PpsI GAGTC 1 cut(s) 892
PpuMI RGGWCCY 1 cut(s) 929
PshBI ATTAAT 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 929
Psp6I CCWGG 1 cut(s) 666
PspFI CCCAGC 1 cut(s) 1194
PspGI CCWGG 1 cut(s) 666
PspN4I GGNNCC 4 cut(s) 48, 930, 931, 1147
PspPI GGNCC 2 cut(s) 929, 1197
PspPPI RGGWCCY 1 cut(s) 929
PstI CTGCAG 1 cut(s) 938
PsuI RGATCY 3 cut(s) 46, 553, 802
RsaI GTAC 3 cut(s) 390, 822, 1225
RsaNI GTAC 3 cut(s) 389, 821, 1224
RseI CAYNNNNRTG 1 cut(s) 828
SaqAI TTAA 5 cut(s) 234, 449, 638, 732, 873
SatI GCNGC 1 cut(s) 1131
Sau3AI GATC 6 cut(s) 46, 410, 553, 802, 983, 1066
Sau96I GGNCC 2 cut(s) 929, 1197
SbfI CCTGCAGG 1 cut(s) 938
SchI GAGTC 1 cut(s) 893
ScrFI CCNGG 1 cut(s) 668
SdaI CCTGCAGG 1 cut(s) 938
SduI GDGCHC 3 cut(s) 458, 568, 957
SfaNI GCATC 2 cut(s) 126, 927
SfcI CTRYAG 1 cut(s) 934
SinI GGWCC 1 cut(s) 929
SmiMI CAYNNNNRTG 1 cut(s) 828
SmlI CTYRAG 4 cut(s) 176, 448, 814, 1039
SmoI CTYRAG 4 cut(s) 176, 448, 814, 1039
SphI GCATGC 1 cut(s) 339
Sse8387I CCTGCAGG 1 cut(s) 938
Sse9I AATT 5 cut(s) 30, 249, 833, 867, 962
SsiI CCGC 1 cut(s) 581
SspMI CTAG 4 cut(s) 140, 644, 1061, 1191
StyD4I CCNGG 1 cut(s) 666
TaaI ACNGT 5 cut(s) 89, 388, 970, 1167, 1186
TaqI TCGA 2 cut(s) 348, 827
TaqII GACCGA 1 cut(s) 606
TasI AATT 5 cut(s) 30, 249, 833, 867, 962
TatI WGTACW 1 cut(s) 820
TfiI GAWTC 3 cut(s) 350, 540, 848
Tru1I TTAA 5 cut(s) 234, 449, 638, 732, 873
Tru9I TTAA 5 cut(s) 234, 449, 638, 732, 873
TscAI CASTG 4 cut(s) 568, 865, 919, 1170
TseFI GTSAC 5 cut(s) 62, 320, 517, 770, 992
TseI GCWGC 1 cut(s) 1130
Tsp45I GTSAC 5 cut(s) 62, 320, 517, 770, 992
TspRI CASTG 4 cut(s) 568, 865, 919, 1170
Van91I CCANNNNNTGG 1 cut(s) 1169
Vha464I CTTAAG 1 cut(s) 448
VpaK11BI GGWCC 1 cut(s) 929
VspI ATTAAT 1 cut(s) 234
XapI RAATTY 1 cut(s) 30
XbaI TCTAGA 1 cut(s) 643
XceI RCATGY 2 cut(s) 339, 1211
XcmI CCANNNNNNNNNTGG 1 cut(s) 529
XmiI GTMKAC 1 cut(s) 1181
XmnI GAANNNNTTC 1 cut(s) 900
XspI CTAG 4 cut(s) 140, 644, 1061, 1191
Zsp2I ATGCAT 1 cut(s) 1213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.