Rw6G025310
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016260
Physical Location & Seq
Forward (+)
48632966 .. 48642643
9678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G025310.1

Sequence Viewer

Length: 4536 bp
ATGGTGGACGAAGCAGGCCTCGCTCGTCGGCTCTGGATCAAGTTCAAAGACGACTCCGTCTTCGCTCTCTACACTCCCTTCTTCGTCGGCCTCGCCTCCGCCACTCTCGACTCTCACACCTTCCGCCATTTCGTCGCTCAGGACCTCCATTTCCTCGAAGCCTTTGCTCAAGCGTACGAATTGGCGGAGGATTGCGCCGACGACGACGACGACAAGGCCGGCATACGCGCTTTGAGGAAGCGCGTTCTCCGGAGGCTTCAATTGCACGATAAACTAATCCGAGATTGGGGAATTGAAGTCCCAGATGAGGGTGTTTCTAATGATGCAGCAACCAAATACACAGATTTCTTGCTTGCAACAGCATCAGGGAAAGTTGAAGGAGGAAGAGTTCCGGGTAAAATTGCTACACCATTTGAAAAGACAAAACTTGCTGCTTATACACTTGCTGCTGTGGCTCCTTTTATGAGACTCTATGCGTATATTAGCAATGAGATTCAGGGTATTCTGGATCCTGATGATAATAGTCACATCTATAAAAGGTGGATAGACAGTTTCTCGTCTAAAGATTTTGAGGCAACAGCTCTACAAAATGAAGAGATGCTAGATAAACTTAGTGTCTCTTTGACTGGAGAGGAGCTTGAGATTTTAGAGAAGCTATATCATCAAGCTATGAAACTTGAAGTAGATTTCATCATTAATAGTGGACTGCCAATTCCTCATAAAGATGTAGTCCCTTTGTCTCGGGTGCATGACTTTACCGACTGCCATCTTACCATACTTTGCGACTTTGACTTGGCATGCACTACTTTCGATTCCTCTGCCATATTAGCAGAGATTGCCATCAGAACAGTACCAAAGGCTGAAACTTATGGATCTGAAACGCAACTTGTCCGGTTCTCTTCAGCAGACTTAAAGAGCACATGGGATATTCTTTCCACCCAATATACAGAAGAGTTTGAACAATGTGTAGAAACCATCGTGACCAGCGAGAAAGTGGAAGGATTCCATTATGAAGATCTATGCAGTGCCCTTGAACAACTTGCGGAGTTTGAGAGACAGGCAAATGAAAGGGTGGTTCAGTCAGGAGTGCTAAAGGGTTTAAATCTAGATGATATAAAACGGGCTGGCCAGGCTCTTATTCTTCAGGATGGTTGTAGAAGCTTCTTTCAGAAGATTGTGAAATATGAAAACCTTAAAAGCGATGTTCATGTACTTTCATACTGCTGGTGTAGTGACCTCATTGTATCAGCTTTTTCTTCAGGAGATCTAAATGCCTTGAGTGTACACTCAAACGAATTGGCTTATGATGAATCGGTTACCACTGGGGAAATTACTAAGAAATTGGAGTCTCCAATGGAAAAGCTTCAAGCTTTCACTGGCATCCTCAAGGGTCCCTGCAGGAGTAGTGAAGACGAGCACAAGCAATTGACAGTTTACATTGGAGGATCAGTTGGTGACTTTCTTTGCCTCCTTCAAGCAGATATTGAAGAACCCAATATGAAGATTTTGCTCATCTTCTTCTTCTTTTACTGTTCCATTGGTTTTGCTTCTTATGGTTCTGCTGCTGTGGCTAACAATGATGAAGCATCTGCCTTGCTTTCGCTAAAAGCAGGTCTGCTTGATCCTTTGGAGAGTCTTAAGGATTGGAAAGTCCAATCTGATCACTGTAACTGGACTGGTGTATGGTGCAATAGTCAGGGTCACGTCGAGAAGCTTGATCTCTCCCACATGAATCTCAGTGGTTCAGTCTCAGGTGACATCCAATGGCTCAAAAGTCTCACTAGTCTCAACTTGTGCTGCAGTGCATTCTCTTCATCATTGCCGAAATCCATGTCGAATCTCACAGCACTGAAGAGCCTTGATGTCAGCCAGAATTCCTTTGTTGGTGATTTTCCAGTGGGACTTGGAAGGGCGGATGGCTTGTCATATCTGAATGCTTCCAGCAACAACTTCTCGGGTTTTCTGCCCGAGGATCTTGGCAATGCCACAATGCTCGAGACTCTGGATCTTCGAGGGAGCTTCTTTGAAGGGGAGATTCCGAAGTCCTTCAGGAAGTTGCAGAAGCTGAAGTTTCTTGGCCTCTCTGGGAATAACCTCACAGGTCAAATCCCATCAGAGCTTGGGAAACTGTTGTCATTGGAGAGCATCATTCTTGGGTATAATGAGTTTCAAGGTGGGATTCCATTGGAATTTGGAAATCTCACCAACTTGAAGTATCTTGATTTGGCAGTTGGCAATCTTAGTGGTGAGATTCCAGCTGAGTTGGGGAAGCTGAAGCAACTAGAGACAGTGTTCTTGTACAAGAACAGCTTTGAAGGTAGAATCCCACCTCAAATCAGCACCATTCCTTCGTTGCAGTTTCTTGACCTATCCGATAACAAGTTATCGGGTGAAATCCCATCAGAGATAGGTGAGCTGGAGAACTTGCAGCTTCTGAATCTCATGTGCAATAAGTTGTCCGGTTCAGTACCATTGGGACTTGGAAACTTGACTCAGTTAGTGGTTCTTGAGCTGTGGAACAATTCTTTATCAGGCTCTTTACCAAGTGATCTTGGTAAAAATGCTCCATTGTTGTGGTTGGATATATCGTCCAACTCATTCTCCGGTGAGATTCCAGCGACATTGTGCAATAAAGGCAATCTCACCAAGCTCATCCTCTTCAACAATGCCTTCACAGGTCCAATTCCAGTTGGCTTGTCAACCTGTGTTTCACTTGTTCGTGTCCGAATGCAGAACAATCTTCTTTCAGGGACAATCCCAGTTGGTCTTGGCAAGCTTGGGAGGCTTCAAAGGTTAGAATTGGCTAATAACAATCTCACTGGTGCAATCCCAGATGACATTTCTTCTTCTACTTCAATTTCTTTCATTGATCTCTCTCGAAACCACCTCCATTCTTCCGTTCCTTCAACAATTCTCTCTGCTCCAAGTTTGCAAACCTTCATGGCCTCGAATAATTTCTTAGTAGGAGAGATTCCAGACCAGTTCCAGGACTGTCCTTCACTTTCTGTGCTTGATCTCTCATCAAACAGTTTCTCTGGAACCATTCCAGCAAGCATTGGTTCATGTGAGAGATTGGTGAATTTGAATCTTGCCAACAACCAATTAACTGGTGAAATCCCCAAGTCAATTGCCATGATGCCCACATTGTCCATTTTGGACTTGTCCAACAACTCTCTCACTGGTGGAATACCAGAAAATTTTGGAATCTCTCCAGCCTTGGAAACTCTCAATGTCTCATACAACAAGCTAGAGGGTGCTATCCCAGCAAATGGTGTGCTTAGAACAATAAACCCAAGTGATCTTGTAGGCAATGCTGGTCTCTGCGGTGGTGTCCTCCCTCCTTGTGCCCGAAATCCAGCATTGGAATCGAGGCATAGGAGCTTGCACACTAAGAATATTATTGCAGGATGGGTGATTGGCATTTCATCAGCTTTAGCAGTTGGAATTGCACTTCTTGGTGCAAGGTCTCTATACAAGAGGTGGTATTCGAATGGAAGTTGCTTCGACGATAGATTCGATGTGGGAAATGGAGAATGGCCATGGAGATTAATGGCATTCCAGAGGCTTGGTTTCACAAGTGCTGACATTTTGGCTTGTGTGAAGGAATCAAATGTGATTGGAATGGGAGCTACTGGCGTTGTCTATAAGGCTGAGATAACAAGATCAAACACAGTTGTTGCAGTTAAGAAGTTGTGGAGACCAGCAACAGATGTTGAAACTGGAAGCAGTGGTGATCTAGTGGGGGAAGTGAATCTCTTGGGGAAGCTAAGGCATCGAAACATTGTTCGGTTACTAGGATTTCTGAACAATGATACCAATTTGATGATTATATATGAGTTTATGAGCAATGGCAGCCTTGGAGAAGCCCTTCATGGCAAGCAAGCTGCGAGGTTGCTTGTAGATTGGGTTACAAGGTATAACATAGTAGTCGGAGTTGCGCAAGGTCTTGCTTATCTCCACCATGATTGTCACCCACCAGTCATTCACAGAGACATCAAGTCCAATAACATACTACTCGATGCAAACCTTGAGGCTAGGATTGCAGATTTCGGGTTGGCAAGGATGATGGTCCGAAAAAATGAGACAGTTTCCATGGTGGCTGGTTCATACGGCTACATTGCCCCTGAATATGGATACACATTGAAGGTTGATGAGAAAATTGACATCTACAGCTATGGTGTGGTTCTATTGGAGCTTCTAACAGGAAAGAGGCCATTAGACCCTGAGTTTGGAGAGTCGGTAGACATCGTGGAATGGATTCGAAGGAAGATTAGGGACAACAAATGTTTAGAAGAAGTATTAGACCCCAGTGTAGGAAATTGCAAGCATGTTCAAGAAGAGATGCTCTTAGTCCTTAGAATAGCACTTGTTTGCACTACCAAGCTTCCCAAGGACAGACCCTCCATGAGAGATGTCATAACCATGCTTGGAGAGGCTAAGCCTCGACGGAAAAGCAGTAGCAACAACAATGATGCATATGCAACTAACAAAGACCAGCCAGTCTTTAGTACCTCACCTGTAAATGGTCTTCTATAG

Protein Analysis

1511

Amino Acids

165.61

Weight (kDa)

5.17

Isoelectric Point (pI)

34.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TENA_THI-4 PF03070 26 - 123 1.4e-06 TENA/THI-4/PQQC family
TENA_THI-4 PF03070 145 - 231 5.3e-07 TENA/THI-4/PQQC family
LRRNT_2 PF08263 525 - 564 7.3e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 641 - 716 1.2e-06 Leucine-rich repeat region
LRR_14 PF23598 731 - 838 1.7e-11 Leucine-rich repeat region
LRR_14 PF23598 825 - 1059 8.9e-08 Leucine-rich repeat region
LRR_8 PF13855 975 - 1035 4.7e-07 Leucine rich repeat
LRR_8 PF13855 1024 - 1083 9.3e-07 Leucine rich repeat
Pkinase PF00069 1202 - 1473 2.4e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1202 - 1475 1e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016260)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20480
malus_domestica MD05G1188600.v1.1
prunus_persica Prupe.8G215100_v2.0.a1 Prupe.8G215100_v2.0.a1
pyrus_communis pycom05g17320
rosa_chinensis RchiOBHm_Chr6g0286581
rosa_laevigata RLG00000012546
rosa_multiflora Rmu_sc0000560.1_g000002
rosa_roxburghii Rroxscaffold_7G00182170
rosa_rugosa Rorug06G0181200
rosa_samantha Rh6AG294100 Rh6BG297200 Rh6CG297600 Rh6DG289500
rosa_wichuraiana Rw6G025310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 56, 4499
Acc16I TGCGCA 1 cut(s) 3939
Acc36I ACCTGC 1 cut(s) 1601
AccB7I CCANNNNNTGG 1 cut(s) 3269
AccI GTMKAC 1 cut(s) 4242
AccII CGCG 2 cut(s) 228, 243
AccIII TCCGGA 1 cut(s) 249
AciI CCGC 6 cut(s) 99, 124, 185, 1043, 1913, 3324
AclWI GGATC 8 cut(s) 44, 503, 516, 880, 1453, 1616, 1980, 2013
AcoI YGGCCR 2 cut(s) 1126, 3536
AcsI RAATTY 4 cut(s) 1873, 2189, 3079, 3196
AcuI CTGAAG 7 cut(s) 885, 1127, 1242, 1871, 2032, 2087, 2294
AfaI GTAC 7 cut(s) 176, 852, 1212, 1284, 2300, 2469, 4510
AfiI CCNNNNNNNGG 9 cut(s) 286, 307, 308, 2986, 3269, 4130, 4229, 4313, 4523
AflII CTTAAG 1 cut(s) 1637
AhdI GACNNNNNGTC 1 cut(s) 3997
AhlI ACTAGT 1 cut(s) 1781
AjiI CACGTC 1 cut(s) 1705
AjnI CCWGG 2 cut(s) 1128, 2985
AloI GAACNNNNNNTCC 2 cut(s) 372, 404
Alw21I GWGCWC 2 cut(s) 920, 1419
AlwI GGATC 8 cut(s) 44, 503, 516, 880, 1453, 1616, 1980, 2013
AlwNI CAGNNNCTG 2 cut(s) 2065, 2434
Ama87I CYCGRG 4 cut(s) 741, 1954, 1967, 1994
Aor13HI TCCGGA 1 cut(s) 249
AoxI GGCC 8 cut(s) 16, 88, 216, 1126, 2077, 2943, 3536, 4211
ApeKI GCWGC 8 cut(s) 326, 431, 446, 1562, 1797, 2428, 3852, 3884
ApoI RAATTY 4 cut(s) 1873, 2189, 3079, 3196
ArsI GACNNNNNNTTYG 8 cut(s) 44, 76, 1817, 1849, 3497, 3529, 4211, 4243
AseI ATTAAT 2 cut(s) 696, 3548
Asp700I GAANNNNTTC 5 cut(s) 1362, 2045, 2954, 3867, 4257
AspLEI GCGC 4 cut(s) 197, 230, 243, 3940
AspS9I GGNCC 4 cut(s) 142, 1391, 2678, 4069
AsuC2I CCSGG 1 cut(s) 393
AsuII TTCGAA 2 cut(s) 3488, 4261
AvaI CYCGRG 4 cut(s) 741, 1954, 1967, 1994
AvaII GGWCC 4 cut(s) 142, 1391, 2678, 4069
BaeGI GKGCMC 2 cut(s) 1030, 3349
BalI TGGCCA 2 cut(s) 1128, 3538
BamHI GGATCC 1 cut(s) 508
BarI GAAGNNNNNNTAC 1 cut(s) 4512
BbsI GAAGAC 3 cut(s) 52, 1416, 4520
Bbv12I GWGCWC 2 cut(s) 920, 1419
BbvI GCAGC 8 cut(s) 338, 418, 433, 1549, 1784, 2440, 3864, 3871
BccI CCATC 9 cut(s) 774, 848, 983, 1142, 1910, 2119, 2407, 3402, 4060
BceAI ACGGC 1 cut(s) 4126
BcgI CGANNNNNNTGC 8 cut(s) 146, 180, 790, 800, 824, 834, 3348, 3382
BciT130I CCWGG 2 cut(s) 1130, 2987
BciVI GTATCC 1 cut(s) 4127
BclI TGATCA 1 cut(s) 1660
BcnI CCSGG 1 cut(s) 393
BcuI ACTAGT 1 cut(s) 1781
BfaI CTAG 8 cut(s) 602, 1106, 1782, 2282, 3248, 3737, 3794, 4035
BfmI CTRYAG 4 cut(s) 1396, 1798, 4168, 4532
BfrI CTTAAG 1 cut(s) 1637
BfuAI ACCTGC 1 cut(s) 1601
BfuI GTATCC 1 cut(s) 4127
BglII AGATCT 2 cut(s) 1015, 1264
BisI GCNGC 8 cut(s) 327, 432, 447, 1563, 1798, 2429, 3853, 3885
BlpI GCTNAGC 1 cut(s) 4437
BlsI GCNGC 8 cut(s) 328, 433, 448, 1564, 1799, 2430, 3854, 3886
Bme1390I CCNGG 3 cut(s) 393, 1130, 2987
Bme18I GGWCC 4 cut(s) 142, 1391, 2678, 4069
BmeRI GACNNNNNGTC 1 cut(s) 3997
BmeT110I CYCGRG 4 cut(s) 741, 1954, 1967, 1994
BmgBI CACGTC 1 cut(s) 1705
BmgT120I GGNCC 4 cut(s) 142, 1391, 2678, 4069
BmiI GGNNCC 5 cut(s) 456, 510, 1392, 1393, 3040
BmrFI CCNGG 3 cut(s) 393, 1130, 2987
BmrI ACTGGG 3 cut(s) 1332, 2753, 4302
BmuI ACTGGG 3 cut(s) 1332, 2753, 4302
BpiI GAAGAC 3 cut(s) 52, 1416, 4520
BpmI CTGGAG 3 cut(s) 648, 2438, 3195
Bpu10I CCTNAGC 2 cut(s) 138, 3767
Bpu1102I GCTNAGC 1 cut(s) 4437
Bpu14I TTCGAA 2 cut(s) 3488, 4261
BpuEI CTTGAG 6 cut(s) 153, 659, 1297, 1370, 2528, 4049
BpuMI CCSGG 1 cut(s) 393
BsaBI GATNNNNATC 1 cut(s) 839
BsaI GGTCTC 3 cut(s) 3323, 3471, 3691
BsaJI CCNNGG 6 cut(s) 1968, 3216, 3539, 3856, 4092, 4389
BsaWI WCCGGW 4 cut(s) 249, 891, 2459, 2603
Bsc4I CCNNNNNNNGG 9 cut(s) 286, 307, 308, 2986, 3269, 4130, 4229, 4313, 4523
Bse118I RCCGGY 1 cut(s) 218
Bse3DI GCAATG 6 cut(s) 493, 1817, 1987, 3316, 3853, 4116
Bse8I GATNNNNATC 1 cut(s) 839
BseAI TCCGGA 1 cut(s) 249
BseBI CCWGG 2 cut(s) 1130, 2987
BseDI CCNNGG 6 cut(s) 1968, 3216, 3539, 3856, 4092, 4389
BseGI GGATG 7 cut(s) 1153, 1380, 1758, 1921, 2652, 3413, 4068
BseJI GATNNNNATC 1 cut(s) 839
BseLI CCNNNNNNNGG 9 cut(s) 286, 307, 308, 2986, 3269, 4130, 4229, 4313, 4523
BseMI GCAATG 6 cut(s) 493, 1817, 1987, 3316, 3853, 4116
BseMII CTCAG 7 cut(s) 152, 1750, 1764, 2250, 2507, 3642, 4215
BseRI GAGGAG 1 cut(s) 647
BseSI GKGCMC 2 cut(s) 1030, 3349
BseXI GCAGC 8 cut(s) 338, 418, 433, 1549, 1784, 2440, 3864, 3871
BseYI CCCAGC 1 cut(s) 3262
Bsh1236I CGCG 2 cut(s) 228, 243
BshFI GGCC 8 cut(s) 18, 90, 218, 1128, 2079, 2945, 3538, 4213
BsiHKAI GWGCWC 2 cut(s) 920, 1419
BsiHKCI CYCGRG 4 cut(s) 741, 1954, 1967, 1994
BsiSI CCGG 6 cut(s) 219, 250, 392, 892, 2460, 2604
BsiWI CGTACG 1 cut(s) 174
BslFI GGGAC 7 cut(s) 284, 716, 1377, 1914, 2490, 2764, 4289
BslI CCNNNNNNNGG 9 cut(s) 286, 307, 308, 2986, 3269, 4130, 4229, 4313, 4523
BsmFI GGGAC 7 cut(s) 284, 716, 1377, 1914, 2490, 2764, 4289
BsmI GAATGC 4 cut(s) 1805, 1939, 2733, 3554
BsnI GGCC 8 cut(s) 18, 90, 218, 1128, 2079, 2945, 3538, 4213
Bso31I GGTCTC 3 cut(s) 3323, 3471, 3691
BsoBI CYCGRG 4 cut(s) 741, 1954, 1967, 1994
Bsp119I TTCGAA 2 cut(s) 3488, 4261
Bsp1286I GDGCHC 4 cut(s) 920, 1030, 1419, 3349
Bsp13I TCCGGA 1 cut(s) 249
Bsp1407I TGTACA 2 cut(s) 1282, 2298
Bsp1720I GCTNAGC 1 cut(s) 4437
Bsp19I CCATGG 2 cut(s) 3539, 4092
BspACI CCGC 6 cut(s) 99, 124, 185, 1043, 1913, 3324
BspANI GGCC 8 cut(s) 18, 90, 218, 1128, 2079, 2945, 3538, 4213
BspCNI CTCAG 7 cut(s) 151, 1749, 1763, 2251, 2506, 3643, 4216
BspEI TCCGGA 1 cut(s) 249
BspFNI CGCG 2 cut(s) 228, 243
BspLI GGNNCC 5 cut(s) 456, 510, 1392, 1393, 3040
BspMAI CTGCAG 2 cut(s) 1400, 1802
BspMI ACCTGC 1 cut(s) 1601
BspPI GGATC 8 cut(s) 44, 503, 516, 880, 1453, 1616, 1980, 2013
BspQI GCTCTTC 1 cut(s) 1847
BspT104I TTCGAA 2 cut(s) 3488, 4261
BspTI CTTAAG 1 cut(s) 1637
BspTNI GGTCTC 3 cut(s) 3323, 3471, 3691
BsrDI GCAATG 6 cut(s) 493, 1817, 1987, 3316, 3853, 4116
BsrFI RCCGGY 1 cut(s) 218
BsrGI TGTACA 2 cut(s) 1282, 2298
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 6 cut(s) 1968, 3216, 3539, 3856, 4092, 4389
BssT1I CCWWGG 5 cut(s) 3216, 3539, 3856, 4092, 4389
Bst2UI CCWGG 2 cut(s) 1130, 2987
Bst6I CTCTTC 8 cut(s) 379, 588, 904, 945, 1816, 1847, 2663, 4332
BstAFI CTTAAG 1 cut(s) 1637
BstAPI GCANNNNNTGC 1 cut(s) 836
BstAUI TGTACA 2 cut(s) 1282, 2298
BstBI TTCGAA 2 cut(s) 3488, 4261
BstDSI CCRYGG 2 cut(s) 3539, 4092
BstEII GGTNACC 1 cut(s) 1315
BstF5I GGATG 7 cut(s) 1153, 1380, 1758, 1921, 2652, 3413, 4068
BstFNI CGCG 2 cut(s) 228, 243
BstHHI GCGC 4 cut(s) 197, 230, 243, 3940
BstNI CCWGG 2 cut(s) 1130, 2987
BstNSI RCATGY 2 cut(s) 801, 4331
BstPI GGTNACC 1 cut(s) 1315
BstSCI CCNGG 3 cut(s) 391, 1128, 2985
BstSFI CTRYAG 4 cut(s) 1396, 1798, 4168, 4532
BstSLI GKGCMC 2 cut(s) 1030, 3349
BstUI CGCG 2 cut(s) 228, 243
BstV1I GCAGC 8 cut(s) 338, 418, 433, 1549, 1784, 2440, 3864, 3871
BstV2I GAAGAC 3 cut(s) 52, 1416, 4520
BstX2I RGATCY 6 cut(s) 508, 872, 1015, 1264, 1972, 2005
BstXI CCANNNNNNTGG 3 cut(s) 2574, 3107, 3566
BstYI RGATCY 6 cut(s) 508, 872, 1015, 1264, 1972, 2005
BsuI GTATCC 1 cut(s) 4127
BsuRI GGCC 8 cut(s) 18, 90, 218, 1128, 2079, 2945, 3538, 4213
BtgI CCRYGG 2 cut(s) 3539, 4092
BtgZI GCGATG 1 cut(s) 1215
BtrI CACGTC 1 cut(s) 1705
BtsCI GGATG 7 cut(s) 1153, 1380, 1758, 1921, 2652, 3413, 4068
BtsI GCAGTG 3 cut(s) 1030, 1807, 3733
BveI ACCTGC 1 cut(s) 1601
CaiI CAGNNNCTG 2 cut(s) 2065, 2434
CfoI GCGC 4 cut(s) 197, 230, 243, 3940
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 4 cut(s) 142, 1391, 2678, 4069
Csp6I GTAC 7 cut(s) 175, 851, 1211, 1283, 2299, 2468, 4509
CspCI CAANNNNNGTGG 4 cut(s) 2224, 2259, 3947, 3982
CviQI GTAC 7 cut(s) 175, 851, 1211, 1283, 2299, 2468, 4509
DraI TTTAAA 1 cut(s) 1101
DrdI GACNNNNNNGTC 2 cut(s) 56, 4499
DriI GACNNNNNGTC 1 cut(s) 3997
DseDI GACNNNNNNGTC 2 cut(s) 56, 4499
EaeI YGGCCR 2 cut(s) 1126, 3536
Eam1104I CTCTTC 8 cut(s) 379, 588, 904, 945, 1816, 1847, 2663, 4332
Eam1105I GACNNNNNGTC 1 cut(s) 3997
EarI CTCTTC 8 cut(s) 379, 588, 904, 945, 1816, 1847, 2663, 4332
EciI GGCGGA 4 cut(s) 88, 113, 200, 1928
Eco130I CCWWGG 5 cut(s) 3216, 3539, 3856, 4092, 4389
Eco147I AGGCCT 1 cut(s) 18
Eco31I GGTCTC 3 cut(s) 3323, 3471, 3691
Eco47I GGWCC 4 cut(s) 142, 1391, 2678, 4069
Eco57I CTGAAG 7 cut(s) 885, 1127, 1242, 1871, 2032, 2087, 2294
Eco88I CYCGRG 4 cut(s) 741, 1954, 1967, 1994
Eco91I GGTNACC 1 cut(s) 1315
EcoO109I RGGNCCY 2 cut(s) 142, 1391
EcoO65I GGTNACC 1 cut(s) 1315
EcoRI GAATTC 1 cut(s) 1873
EcoRII CCWGG 2 cut(s) 1128, 2985
EcoT14I CCWWGG 5 cut(s) 3216, 3539, 3856, 4092, 4389
EcoT22I ATGCAT 1 cut(s) 4477
ErhI CCWWGG 5 cut(s) 3216, 3539, 3856, 4092, 4389
FalI AAGNNNNNCTT 2 cut(s) 2294, 2326
FaqI GGGAC 7 cut(s) 284, 716, 1377, 1914, 2490, 2764, 4289
FauNDI CATATG 1 cut(s) 4477
FbaI TGATCA 1 cut(s) 1660
FblI GTMKAC 1 cut(s) 4242
Fnu4HI GCNGC 8 cut(s) 327, 432, 447, 1563, 1798, 2429, 3853, 3885
FokI GGATG 7 cut(s) 1160, 1367, 1745, 1928, 2639, 3420, 4075
Fsp4HI GCNGC 8 cut(s) 327, 432, 447, 1563, 1798, 2429, 3853, 3885
FspBI CTAG 8 cut(s) 602, 1106, 1782, 2282, 3248, 3737, 3794, 4035
FspI TGCGCA 1 cut(s) 3939
GlaI GCGC 4 cut(s) 196, 229, 242, 3939
GluI GCNGC 8 cut(s) 327, 432, 447, 1563, 1798, 2429, 3853, 3885
GsaI CCCAGC 1 cut(s) 3266
GsuI CTGGAG 3 cut(s) 648, 2438, 3195
HaeIII GGCC 8 cut(s) 18, 90, 218, 1128, 2079, 2945, 3538, 4213
HapII CCGG 6 cut(s) 219, 250, 392, 892, 2460, 2604
HhaI GCGC 4 cut(s) 197, 230, 243, 3940
Hin6I GCGC 4 cut(s) 195, 228, 241, 3938
HinP1I GCGC 4 cut(s) 195, 228, 241, 3938
HincII GTYRAC 1 cut(s) 2700
HindII GTYRAC 1 cut(s) 2700
HindIII AAGCTT 6 cut(s) 1159, 1361, 1368, 1712, 2774, 4382
HpaII CCGG 6 cut(s) 219, 250, 392, 892, 2460, 2604
Hpy166II GTNNAC 7 cut(s) 7, 704, 1283, 1285, 1435, 2700, 4243
Hpy8I GTNNAC 7 cut(s) 7, 704, 1283, 1285, 1435, 2700, 4243
HpyCH4IV ACGT 1 cut(s) 1704
HpySE526I ACGT 1 cut(s) 1704
HspAI GCGC 4 cut(s) 195, 228, 241, 3938
KflI GGGWCCC 1 cut(s) 1391
Kpn2I TCCGGA 1 cut(s) 249
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 1660
LguI GCTCTTC 1 cut(s) 1847
LmnI GCTCC 8 cut(s) 460, 634, 2016, 2569, 2926, 3378, 3626, 4192
Lsp1109I GCAGC 8 cut(s) 338, 418, 433, 1549, 1784, 2440, 3864, 3871
MaeI CTAG 8 cut(s) 602, 1106, 1782, 2282, 3248, 3737, 3794, 4035
MaeII ACGT 1 cut(s) 1704
MfeI CAATTG 3 cut(s) 260, 1424, 3126
MflI RGATCY 6 cut(s) 508, 872, 1015, 1264, 1972, 2005
MhlI GDGCHC 4 cut(s) 920, 1030, 1419, 3349
MlsI TGGCCA 2 cut(s) 1128, 3538
MluNI TGGCCA 2 cut(s) 1128, 3538
MlyI GAGTC 8 cut(s) 47, 104, 462, 1355, 1642, 1993, 2485, 4244
MmeI TCCRAC 5 cut(s) 2559, 2616, 3189, 3421, 3910
Mox20I TGGCCA 2 cut(s) 1128, 3538
Mph1103I ATGCAT 1 cut(s) 4477
MroI TCCGGA 1 cut(s) 249
MroNI GCCGGC 1 cut(s) 218
MroXI GAANNNNTTC 5 cut(s) 1362, 2045, 2954, 3867, 4257
MscI TGGCCA 2 cut(s) 1128, 3538
MseI TTAA 8 cut(s) 696, 911, 1100, 1194, 1638, 3104, 3548, 3684
MslI CAYNNNNRTG 3 cut(s) 723, 2572, 4421
Msp20I TGGCCA 2 cut(s) 1128, 3538
MspA1I CMGCKG 1 cut(s) 2258
MspCI CTTAAG 1 cut(s) 1637
MspI CCGG 6 cut(s) 219, 250, 392, 892, 2460, 2604
MspR9I CCNGG 3 cut(s) 393, 1130, 2987
MunI CAATTG 3 cut(s) 260, 1424, 3126
Mva1269I GAATGC 4 cut(s) 1805, 1939, 2733, 3554
MvaI CCWGG 2 cut(s) 1130, 2987
MvnI CGCG 2 cut(s) 228, 243
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 1 cut(s) 393
NcoI CCATGG 2 cut(s) 3539, 4092
NdeI CATATG 1 cut(s) 4477
NgoMIV GCCGGC 1 cut(s) 218
NlaIV GGNNCC 5 cut(s) 456, 510, 1392, 1393, 3040
NmuCI GTSAC 7 cut(s) 524, 979, 1232, 1454, 1700, 1754, 3968
NsbI TGCGCA 1 cut(s) 3939
NsiI ATGCAT 1 cut(s) 4477
NspI RCATGY 2 cut(s) 801, 4331
NspV TTCGAA 2 cut(s) 3488, 4261
PaeI GCATGC 1 cut(s) 801
PaeR7I CTCGAG 1 cut(s) 1994
PceI AGGCCT 1 cut(s) 18
PciSI GCTCTTC 1 cut(s) 1847
PcsI WCGNNNNNNNCGW 3 cut(s) 207, 984, 3513
PctI GAATGC 4 cut(s) 1805, 1939, 2733, 3554
PdiI GCCGGC 1 cut(s) 220
PdmI GAANNNNTTC 5 cut(s) 1362, 2045, 2954, 3867, 4257
Pfl23II CGTACG 1 cut(s) 174
PflFI GACNNNGTC 1 cut(s) 56
PflMI CCANNNNNTGG 1 cut(s) 3269
PfoI TCCNGGA 1 cut(s) 2985
PkrI GCNGC 8 cut(s) 328, 433, 448, 1564, 1799, 2430, 3854, 3886
PleI GAGTC 8 cut(s) 47, 104, 462, 1354, 1641, 1993, 2485, 4243
PpsI GAGTC 8 cut(s) 47, 104, 462, 1354, 1641, 1993, 2485, 4243
PpuMI RGGWCCY 2 cut(s) 142, 1391
PshBI ATTAAT 2 cut(s) 696, 3548
Psp5II RGGWCCY 2 cut(s) 142, 1391
Psp6I CCWGG 2 cut(s) 1128, 2985
PspEI GGTNACC 1 cut(s) 1315
PspFI CCCAGC 1 cut(s) 3262
PspGI CCWGG 2 cut(s) 1128, 2985
PspLI CGTACG 1 cut(s) 174
PspN4I GGNNCC 5 cut(s) 456, 510, 1392, 1393, 3040
PspPI GGNCC 4 cut(s) 142, 1391, 2678, 4069
PspPPI RGGWCCY 2 cut(s) 142, 1391
PstI CTGCAG 2 cut(s) 1400, 1802
PstNI CAGNNNCTG 2 cut(s) 2065, 2434
PsuI RGATCY 6 cut(s) 508, 872, 1015, 1264, 1972, 2005
PsyI GACNNNGTC 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 2258
RsaI GTAC 7 cut(s) 176, 852, 1212, 1284, 2300, 2469, 4510
RsaNI GTAC 7 cut(s) 175, 851, 1211, 1283, 2299, 2468, 4509
RseI CAYNNNNRTG 3 cut(s) 723, 2572, 4421
SapI GCTCTTC 1 cut(s) 1847
SaqAI TTAA 8 cut(s) 696, 911, 1100, 1194, 1638, 3104, 3548, 3684
SatI GCNGC 8 cut(s) 327, 432, 447, 1563, 1798, 2429, 3853, 3885
Sau96I GGNCC 4 cut(s) 142, 1391, 2678, 4069
SbfI CCTGCAGG 1 cut(s) 1400
SchI GAGTC 8 cut(s) 47, 104, 462, 1355, 1642, 1993, 2485, 4244
ScrFI CCNGG 3 cut(s) 393, 1130, 2987
SdaI CCTGCAGG 1 cut(s) 1400
SduI GDGCHC 4 cut(s) 920, 1030, 1419, 3349
SfcI CTRYAG 4 cut(s) 1396, 1798, 4168, 4532
Sfr274I CTCGAG 1 cut(s) 1994
SfuI TTCGAA 2 cut(s) 3488, 4261
SinI GGWCC 4 cut(s) 142, 1391, 2678, 4069
SlaI CTCGAG 1 cut(s) 1994
SmiMI CAYNNNNRTG 3 cut(s) 723, 2572, 4421
SmlI CTYRAG 8 cut(s) 168, 638, 1276, 1385, 1637, 1994, 2507, 4028
SmoI CTYRAG 8 cut(s) 168, 638, 1276, 1385, 1637, 1994, 2507, 4028
SpeI ACTAGT 1 cut(s) 1781
SphI GCATGC 1 cut(s) 801
Sse8387I CCTGCAGG 1 cut(s) 1400
SseBI AGGCCT 1 cut(s) 18
SsiI CCGC 6 cut(s) 99, 124, 185, 1043, 1913, 3324
SspI AATATT 1 cut(s) 3397
SspMI CTAG 8 cut(s) 602, 1106, 1782, 2282, 3248, 3737, 3794, 4035
StuI AGGCCT 1 cut(s) 18
StyD4I CCNGG 3 cut(s) 391, 1128, 2985
StyI CCWWGG 5 cut(s) 3216, 3539, 3856, 4092, 4389
TaiI ACGT 1 cut(s) 1707
TatI WGTACW 3 cut(s) 1210, 1282, 2298
Tru1I TTAA 8 cut(s) 696, 911, 1100, 1194, 1638, 3104, 3548, 3684
Tru9I TTAA 8 cut(s) 696, 911, 1100, 1194, 1638, 3104, 3548, 3684
TseFI GTSAC 7 cut(s) 524, 979, 1232, 1454, 1700, 1754, 3968
TseI GCWGC 8 cut(s) 326, 431, 446, 1562, 1797, 2428, 3852, 3884
Tsp45I GTSAC 7 cut(s) 524, 979, 1232, 1454, 1700, 1754, 3968
TspGWI ACGGA 3 cut(s) 46, 2887, 4462
Tth111I GACNNNGTC 1 cut(s) 56
Van91I CCANNNNNTGG 1 cut(s) 3269
Vha464I CTTAAG 1 cut(s) 1637
VpaK11BI GGWCC 4 cut(s) 142, 1391, 2678, 4069
VspI ATTAAT 2 cut(s) 696, 3548
XapI RAATTY 4 cut(s) 1873, 2189, 3079, 3196
XbaI TCTAGA 1 cut(s) 1105
XceI RCATGY 2 cut(s) 801, 4331
XcmI CCANNNNNNNNNTGG 1 cut(s) 991
XhoI CTCGAG 1 cut(s) 1994
XmiI GTMKAC 1 cut(s) 4242
XmnI GAANNNNTTC 5 cut(s) 1362, 2045, 2954, 3867, 4257
XspI CTAG 8 cut(s) 602, 1106, 1782, 2282, 3248, 3737, 3794, 4035
Zsp2I ATGCAT 1 cut(s) 4477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.