RLG00000012774

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018201
Physical Location & Seq
Reverse (-)
22231970 .. 22233699
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012774

Sequence Viewer

Length: 1170 bp
ATGGTGAGCTTAAGAAGACACAAACGCTCAAGCCTATCCCCTGATGGAAGTAGTTATGTTCTAGCTCCACTTACTGGGTTCACTGACAGCGAAATTGACACTCATGCAAAACTTGATTGTGTGCATCCCATGCCTTTGAATGATGAAGATGAAGCAAAGGAGCTGAACTTACCCAAAGAGACCAAGACAGTTTCAGTACTAAACAGTGCCAAGAGGAAAATGAGGAAGATTGATGAGTTGATGAAGATTACTGAAACTGAAGTCATCGAGTCACCTAAAAATTACCAGGTTGACTTGAAAAAACCAGTGCGTCGATGGAAGAAAGTGCTGGAAAAGAAGAAGTCCCCGAAGGTGTCAGAGGTTACCAAAGATGAACTTGAGAAGATAGTGAAGAAACTAAGGACAAACTCTGACTTCTACCTGGAGGAACTGGATCTGCTCCACAAGAAGAAGGAAAATGAGAGCAAGCTAACAACCGAGCTGATCAAGACCTGCCAAAAAGAGTTGAAAGAGGCTGAAGACAATAATCATGCTTTCTCGTTGGATTACTGTCTCCTGGATAAGTCCTATGTCCAGTCTTTGGAAAATATTTGGCTAGAAGGTGAAGACCAGAATGTGGATGACGAGGGCACTGATACATTGGGGAATGAAGAAGCGAAGAATAAGATTGACATCTCGGAAGATTGGCTGAATAAGCTAAAGGAGAAGAAGGGACTGCAAAAGGCTGAGCTGGACGCTGCAAAGGTGGAAACAGAGAAGCTGCGTAGATATGTTGTTGAGCTCGACAGGGTGGCTGTGGACATGGCCAAAGCTCAAGCTGCTGCCATCAAAGCTGAGAAGGACGAGGTTGAAGAGCAAAATGAAGAAATGAAGAACAAGATTGACATCTTGAAAACCATGGTGGAAAAACTAAAGGACAAGAAGATACTGCAAAAATCTGAGGCTGAAGCTGCAATGGTGGCTTGTGAGAAGGTATGCAGAGATGTAGAGCTTAGCAGGGTGGCTGTGGACATGGCCGAAGCTGAAACTTCTACCCTCAAAGCTGAGAAGGACAAGCTTGACGAGCAAAATGAAATCGAGATGAGGAAAAGGAGGAAATATATTGCTGCTCTGATCTCTGAGCTGAAAAGTTGCTACTCCTTCCGAACAGATCCGAAGATGAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

390

Amino Acids

44.79

Weight (kDa)

5.66

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018201)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0284051
rosa_laevigata RLG00000012774 RLG00000012777
rosa_roxburghii Rroxscaffold_7G00184980
rosa_rugosa Rorug06G0159900 Rorug06G0159900 Rorug06G0160000
rosa_samantha Rh6BG273600 Rh6CG275100 Rh6DG268000
rosa_wichuraiana Rw6G023500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 500
AccB7I CCANNNNNTGG 3 cut(s) 74, 580, 616
AclWI GGATC 2 cut(s) 441, 1145
AcoI YGGCCR 2 cut(s) 804, 1014
AcuI CTGAAG 3 cut(s) 279, 537, 966
AfaI GTAC 1 cut(s) 198
AfiI CCNNNNNNNGG 3 cut(s) 74, 580, 616
AflII CTTAAG 1 cut(s) 10
AgsI TTSAA 5 cut(s) 139, 298, 508, 851, 892
AjnI CCWGG 3 cut(s) 285, 420, 555
Alw21I GWGCWC 2 cut(s) 783, 1166
Alw26I GTCTC 2 cut(s) 173, 557
AlwI GGATC 2 cut(s) 441, 1145
AoxI GGCC 2 cut(s) 804, 1014
ApeKI GCWGC 6 cut(s) 737, 760, 818, 821, 950, 1106
AsuHPI GGTGA 3 cut(s) 16, 264, 614
BaeGI GKGCMC 1 cut(s) 632
BalI TGGCCA 1 cut(s) 806
BanII GRGCYC 2 cut(s) 783, 1166
BbsI GAAGAC 3 cut(s) 22, 525, 612
Bbv12I GWGCWC 2 cut(s) 783, 1166
BbvI GCAGC 6 cut(s) 724, 747, 805, 808, 937, 1093
BccI CCATC 3 cut(s) 38, 309, 833
BciT130I CCWGG 3 cut(s) 287, 422, 557
BclI TGATCA 1 cut(s) 483
BcoDI GTCTC 2 cut(s) 173, 557
BfaI CTAG 2 cut(s) 62, 596
BfrI CTTAAG 1 cut(s) 10
BfuAI ACCTGC 1 cut(s) 500
BisI GCNGC 6 cut(s) 738, 761, 819, 822, 951, 1107
BlpI GCTNAGC 2 cut(s) 726, 992
BlsI GCNGC 6 cut(s) 739, 762, 820, 823, 952, 1108
BmcAI AGTACT 1 cut(s) 198
Bme1390I CCNGG 3 cut(s) 287, 422, 557
BmrFI CCNGG 3 cut(s) 287, 422, 557
BmrI ACTGGG 1 cut(s) 84
BmsI GCATC 1 cut(s) 133
BmuI ACTGGG 1 cut(s) 84
BpiI GAAGAC 3 cut(s) 22, 525, 612
BpmI CTGGAG 1 cut(s) 443
Bpu1102I GCTNAGC 2 cut(s) 726, 992
BpuEI CTTGAG 3 cut(s) 13, 398, 798
BsaBI GATNNNNATC 2 cut(s) 671, 884
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 1 cut(s) 897
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 580, 616
Bse1I ACTGG 4 cut(s) 79, 305, 435, 574
Bse3DI GCAATG 1 cut(s) 960
Bse8I GATNNNNATC 2 cut(s) 671, 884
BseBI CCWGG 3 cut(s) 287, 422, 557
BseDI CCNNGG 1 cut(s) 897
BseGI GGATG 2 cut(s) 124, 625
BseJI GATNNNNATC 2 cut(s) 671, 884
BseLI CCNNNNNNNGG 3 cut(s) 74, 580, 616
BseMI GCAATG 1 cut(s) 960
BseMII CTCAG 5 cut(s) 717, 825, 930, 1035, 1110
BseNI ACTGG 4 cut(s) 79, 305, 435, 574
BseSI GKGCMC 1 cut(s) 632
BseXI GCAGC 6 cut(s) 724, 747, 805, 808, 937, 1093
BshFI GGCC 2 cut(s) 806, 1016
BsiHKAI GWGCWC 2 cut(s) 783, 1166
BslFI GGGAC 2 cut(s) 328, 726
BslI CCNNNNNNNGG 3 cut(s) 74, 580, 616
BsmAI GTCTC 2 cut(s) 173, 557
BsmFI GGGAC 2 cut(s) 328, 726
BsnI GGCC 2 cut(s) 806, 1016
Bso31I GGTCTC 1 cut(s) 173
Bsp1286I GDGCHC 3 cut(s) 632, 783, 1166
Bsp143I GATC 4 cut(s) 433, 483, 1113, 1150
Bsp1720I GCTNAGC 2 cut(s) 726, 992
Bsp19I CCATGG 1 cut(s) 897
BspANI GGCC 2 cut(s) 806, 1016
BspCNI CTCAG 5 cut(s) 718, 826, 931, 1036, 1111
BspMI ACCTGC 1 cut(s) 500
BspPI GGATC 2 cut(s) 441, 1145
BspQI GCTCTTC 1 cut(s) 846
BspTI CTTAAG 1 cut(s) 10
BspTNI GGTCTC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 960
BsrI ACTGG 4 cut(s) 79, 305, 435, 574
BssECI CCNNGG 1 cut(s) 897
BssMI GATC 4 cut(s) 433, 483, 1113, 1150
BssT1I CCWWGG 1 cut(s) 897
Bst2UI CCWGG 3 cut(s) 287, 422, 557
Bst4CI ACNGT 3 cut(s) 190, 206, 551
Bst6I CTCTTC 1 cut(s) 846
BstAFI CTTAAG 1 cut(s) 10
BstAPI GCANNNNNTGC 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 467
BstDEI CTNAG 7 cut(s) 398, 726, 834, 939, 992, 1044, 1119
BstDSI CCRYGG 1 cut(s) 897
BstEII GGTNACC 1 cut(s) 361
BstF5I GGATG 2 cut(s) 124, 625
BstKTI GATC 4 cut(s) 436, 486, 1116, 1153
BstMAI GTCTC 2 cut(s) 173, 557
BstMBI GATC 4 cut(s) 433, 483, 1113, 1150
BstMWI GCNNNNNNNGC 7 cut(s) 130, 694, 818, 830, 950, 959, 1063
BstNI CCWGG 3 cut(s) 287, 422, 557
BstPI GGTNACC 1 cut(s) 361
BstSCI CCNGG 3 cut(s) 285, 420, 555
BstSLI GKGCMC 1 cut(s) 632
BstV1I GCAGC 6 cut(s) 724, 747, 805, 808, 937, 1093
BstV2I GAAGAC 3 cut(s) 22, 525, 612
BstX2I RGATCY 2 cut(s) 433, 1150
BstYI RGATCY 2 cut(s) 433, 1150
BsuRI GGCC 2 cut(s) 806, 1016
BtgI CCRYGG 1 cut(s) 897
BtsCI GGATG 2 cut(s) 124, 625
BtsIMutI CAGTG 4 cut(s) 81, 211, 312, 630
BveI ACCTGC 1 cut(s) 500
Cac8I GCNNGC 1 cut(s) 467
CseI GACGC 2 cut(s) 299, 743
CsiI ACCWGGT 1 cut(s) 285
Csp6I GTAC 1 cut(s) 197
CviAII CATG 6 cut(s) 104, 130, 530, 802, 898, 1012
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 7 cut(s) 398, 726, 834, 939, 992, 1044, 1119
DpnI GATC 4 cut(s) 435, 485, 1115, 1152
DpnII GATC 4 cut(s) 433, 483, 1113, 1150
EaeI YGGCCR 2 cut(s) 804, 1014
Eam1104I CTCTTC 1 cut(s) 846
EarI CTCTTC 1 cut(s) 846
Ecl136II GAGCTC 2 cut(s) 781, 1164
Eco130I CCWWGG 1 cut(s) 897
Eco24I GRGCYC 2 cut(s) 783, 1166
Eco31I GGTCTC 1 cut(s) 173
Eco53kI GAGCTC 2 cut(s) 781, 1164
Eco57I CTGAAG 3 cut(s) 279, 537, 966
Eco91I GGTNACC 1 cut(s) 361
EcoICRI GAGCTC 2 cut(s) 781, 1164
EcoO65I GGTNACC 1 cut(s) 361
EcoRII CCWGG 3 cut(s) 285, 420, 555
EcoT14I CCWWGG 1 cut(s) 897
EcoT38I GRGCYC 2 cut(s) 783, 1166
ErhI CCWWGG 1 cut(s) 897
FaeI CATG 6 cut(s) 107, 133, 533, 805, 901, 1015
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FaqI GGGAC 2 cut(s) 328, 726
FatI CATG 6 cut(s) 103, 129, 529, 801, 897, 1011
FbaI TGATCA 1 cut(s) 483
Fnu4HI GCNGC 6 cut(s) 738, 761, 819, 822, 951, 1107
FokI GGATG 2 cut(s) 111, 632
FriOI GRGCYC 2 cut(s) 783, 1166
Fsp4HI GCNGC 6 cut(s) 738, 761, 819, 822, 951, 1107
FspBI CTAG 2 cut(s) 62, 596
GluI GCNGC 6 cut(s) 738, 761, 819, 822, 951, 1107
GsuI CTGGAG 1 cut(s) 443
HaeIII GGCC 2 cut(s) 806, 1016
HgaI GACGC 2 cut(s) 299, 743
Hin1II CATG 6 cut(s) 107, 133, 533, 805, 901, 1015
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HindIII AAGCTT 1 cut(s) 1055
HinfI GANTC 1 cut(s) 269
HphI GGTGA 3 cut(s) 16, 264, 614
Hpy166II GTNNAC 4 cut(s) 81, 292, 799, 1009
Hpy188I TCNGA 8 cut(s) 358, 412, 679, 940, 1113, 1120, 1145, 1155
Hpy188III TCNNGA 4 cut(s) 487, 889, 1078, 1167
Hpy8I GTNNAC 4 cut(s) 81, 292, 799, 1009
Hpy99I CGWCG 1 cut(s) 315
HpyAV CCTTC 8 cut(s) 343, 445, 593, 703, 832, 964, 1042, 1150
HpyCH4III ACNGT 3 cut(s) 190, 206, 551
HpyCH4V TGCA 7 cut(s) 107, 124, 718, 740, 931, 953, 978
HpyF10VI GCNNNNNNNGC 7 cut(s) 130, 694, 818, 830, 950, 959, 1063
HpyF3I CTNAG 7 cut(s) 398, 726, 834, 939, 992, 1044, 1119
Hsp92II CATG 6 cut(s) 107, 133, 533, 805, 901, 1015
Ksp22I TGATCA 1 cut(s) 483
Kzo9I GATC 4 cut(s) 433, 483, 1113, 1150
LguI GCTCTTC 1 cut(s) 846
LmnI GCTCC 3 cut(s) 70, 160, 444
Lsp1109I GCAGC 6 cut(s) 724, 747, 805, 808, 937, 1093
LweI GCATC 1 cut(s) 133
MabI ACCWGGT 1 cut(s) 285
MaeI CTAG 2 cut(s) 62, 596
MaeIII GTNAC 2 cut(s) 270, 361
MalI GATC 4 cut(s) 435, 485, 1115, 1152
MboI GATC 4 cut(s) 433, 483, 1113, 1150
MflI RGATCY 2 cut(s) 433, 1150
MhlI GDGCHC 3 cut(s) 632, 783, 1166
MlsI TGGCCA 1 cut(s) 806
MluCI AATT 2 cut(s) 93, 280
MluNI TGGCCA 1 cut(s) 806
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 522
Mox20I TGGCCA 1 cut(s) 806
MscI TGGCCA 1 cut(s) 806
MseI TTAA 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 806
MspCI CTTAAG 1 cut(s) 10
MspR9I CCNGG 3 cut(s) 287, 422, 557
MvaI CCWGG 3 cut(s) 287, 422, 557
MwoI GCNNNNNNNGC 7 cut(s) 130, 694, 818, 830, 950, 959, 1063
NcoI CCATGG 1 cut(s) 897
NdeII GATC 4 cut(s) 433, 483, 1113, 1150
NlaIII CATG 6 cut(s) 107, 133, 533, 805, 901, 1015
NmuCI GTSAC 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 846
PflMI CCANNNNNTGG 3 cut(s) 74, 580, 616
PfoI TCCNGGA 1 cut(s) 555
PkrI GCNGC 6 cut(s) 739, 762, 820, 823, 952, 1108
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
Psp124BI GAGCTC 2 cut(s) 783, 1166
Psp6I CCWGG 3 cut(s) 285, 420, 555
PspEI GGTNACC 1 cut(s) 361
PspGI CCWGG 3 cut(s) 285, 420, 555
PsuI RGATCY 2 cut(s) 433, 1150
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
SacI GAGCTC 2 cut(s) 783, 1166
SapI GCTCTTC 1 cut(s) 846
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 6 cut(s) 738, 761, 819, 822, 951, 1107
Sau3AI GATC 4 cut(s) 433, 483, 1113, 1150
ScaI AGTACT 1 cut(s) 198
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 3 cut(s) 287, 422, 557
SduI GDGCHC 3 cut(s) 632, 783, 1166
SexAI ACCWGGT 1 cut(s) 285
SfaNI GCATC 1 cut(s) 133
SmlI CTYRAG 4 cut(s) 10, 28, 377, 813
SmoI CTYRAG 4 cut(s) 10, 28, 377, 813
Sse9I AATT 2 cut(s) 93, 280
SspI AATATT 1 cut(s) 589
SspMI CTAG 2 cut(s) 62, 596
SstI GAGCTC 2 cut(s) 783, 1166
StyD4I CCNGG 3 cut(s) 285, 420, 555
StyI CCWWGG 1 cut(s) 897
TaaI ACNGT 3 cut(s) 190, 206, 551
TaqI TCGA 4 cut(s) 267, 313, 783, 1077
TasI AATT 2 cut(s) 93, 280
TatI WGTACW 1 cut(s) 196
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TscAI CASTG 4 cut(s) 88, 211, 312, 637
TseFI GTSAC 1 cut(s) 270
TseI GCWGC 6 cut(s) 737, 760, 818, 821, 950, 1106
Tsp45I GTSAC 1 cut(s) 270
TspDTI ATGAA 8 cut(s) 159, 165, 257, 387, 663, 876, 884, 1086
TspRI CASTG 4 cut(s) 88, 211, 312, 637
Van91I CCANNNNNTGG 3 cut(s) 74, 580, 616
Vha464I CTTAAG 1 cut(s) 10
XcmI CCANNNNNNNNNTGG 1 cut(s) 312
XspI CTAG 2 cut(s) 62, 596
ZrmI AGTACT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.