RLG00000012777

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018201
Physical Location & Seq
Reverse (-)
22257461 .. 22259190
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012777

Sequence Viewer

Length: 1179 bp
ATGGTGAGCTTAAGAAGACACAAACGCTCAAGCCTATCCCCTGTCTTTGCAGATGGAAGTAGTTATGTTCTAGCTCCACTTACTGGGTTCACTGACAGCGAAATTGACACTCATGCAAAACTTGATTGTGTGCATCCCATGCCTTTGAATGATGAAGATGAAGCAAAGGAGCTGAACTTACCCAAAGAGACCAAGACAGTTTCAGTACTAAACAGTGCCAAGAGGAAAATGAGGAAGATTGATGAGTTGATGAAGATTACTGAAACTGAAGTCATCGAGTCACCTAAAAATTACCAGGTTGACTTGAAAAAACCAGTGCGTCGATGGAAGAAAGTGCTGGAAAAGAAGAAGTCCCCGAAGGTGTCAGAGGTTACCAAAGATGAACTTGAGAAGATAGTGAAGAAACTAAGGACAAACTCTGACTTCTACCTGGAGGAACTGGATCTGCTCCACAAGAAGAAGGAAAATGAGAGCAAGCTAACAACCGAGCTGATCAAGACCTGCCAAAAAGAGTTGAAAGAGGCTGAAGACAATAATCATGCTTTCTCGTTGGATTACTGTCTCCTGGATAAGTCCTATGTCCAGTCTTTGGAAAATATTTGGCTAGAAGGTGAAGACCAGAATGTGGATGACGAGGGCACTGATACATTGGGGAATGAAGAAGCGAAGAATAAGATTGACATCTCGGAAGATTGGCTGAATAAGCTAAAGGAGAAGAAGGGACTGCAAAAGGCTGAGCTGGACGCTGCAAAGGTGGAAACAGAGAAGCTGCGTAGATATGTTGTTGAGCTCGACAGGGTGGCTGTGGACATGGCCAAAGCTCAAGCTGCTGCCATCAAAGCTGAGAAGGACGAGGTTGAAGAGCAAAATGAAGAAATGAAGAACAAGATTGACATCTTGAAAACCATGGTGGAAAAACTAAAGGACAAGAAGATACTGCAAAAATCTGAGGCTGAAGCTGCAATGGTGGCTTGTGAGAAGGTATGCAGAGATGTAGAGCTTAGCAGGGTGGCTGTGGACATGGCCGAAGCTGAAACTTCTACCCTCAAAGCTGAGAAGGACAAGCTTGACGAGCAAAATGAAATCGAGATGAGGAAAAGGAGGAAATATATTGCTGCTCTGATCTCTGAGCTGAAAAGTTGCTACTCCTTCCGAACAGATCCGAAGATGAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

393

Amino Acids

45.1

Weight (kDa)

5.66

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018201)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0284051
rosa_laevigata RLG00000012774 RLG00000012777
rosa_roxburghii Rroxscaffold_7G00184980
rosa_rugosa Rorug06G0159900 Rorug06G0159900 Rorug06G0160000
rosa_samantha Rh6BG273600 Rh6CG275100 Rh6DG268000
rosa_wichuraiana Rw6G023500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 509
AccB7I CCANNNNNTGG 3 cut(s) 83, 589, 625
AclWI GGATC 2 cut(s) 450, 1154
AcoI YGGCCR 2 cut(s) 813, 1023
AcuI CTGAAG 3 cut(s) 288, 546, 975
AfaI GTAC 1 cut(s) 207
AfiI CCNNNNNNNGG 3 cut(s) 83, 589, 625
AflII CTTAAG 1 cut(s) 10
AgsI TTSAA 5 cut(s) 148, 307, 517, 860, 901
AjnI CCWGG 3 cut(s) 294, 429, 564
Alw21I GWGCWC 2 cut(s) 792, 1175
Alw26I GTCTC 2 cut(s) 182, 566
AlwI GGATC 2 cut(s) 450, 1154
AoxI GGCC 2 cut(s) 813, 1023
ApeKI GCWGC 6 cut(s) 746, 769, 827, 830, 959, 1115
AsuHPI GGTGA 3 cut(s) 16, 273, 623
BaeGI GKGCMC 1 cut(s) 641
BalI TGGCCA 1 cut(s) 815
BanII GRGCYC 2 cut(s) 792, 1175
BbsI GAAGAC 3 cut(s) 22, 534, 621
Bbv12I GWGCWC 2 cut(s) 792, 1175
BbvI GCAGC 6 cut(s) 733, 756, 814, 817, 946, 1102
BccI CCATC 3 cut(s) 47, 318, 842
BciT130I CCWGG 3 cut(s) 296, 431, 566
BclI TGATCA 1 cut(s) 492
BcoDI GTCTC 2 cut(s) 182, 566
BfaI CTAG 2 cut(s) 71, 605
BfrI CTTAAG 1 cut(s) 10
BfuAI ACCTGC 1 cut(s) 509
BisI GCNGC 6 cut(s) 747, 770, 828, 831, 960, 1116
BlpI GCTNAGC 2 cut(s) 735, 1001
BlsI GCNGC 6 cut(s) 748, 771, 829, 832, 961, 1117
BmcAI AGTACT 1 cut(s) 207
Bme1390I CCNGG 3 cut(s) 296, 431, 566
BmrFI CCNGG 3 cut(s) 296, 431, 566
BmrI ACTGGG 1 cut(s) 93
BmsI GCATC 1 cut(s) 142
BmuI ACTGGG 1 cut(s) 93
BpiI GAAGAC 3 cut(s) 22, 534, 621
BpmI CTGGAG 1 cut(s) 452
Bpu1102I GCTNAGC 2 cut(s) 735, 1001
BpuEI CTTGAG 3 cut(s) 13, 407, 807
BsaBI GATNNNNATC 2 cut(s) 680, 893
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 906
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 589, 625
Bse1I ACTGG 4 cut(s) 88, 314, 444, 583
Bse3DI GCAATG 1 cut(s) 969
Bse8I GATNNNNATC 2 cut(s) 680, 893
BseBI CCWGG 3 cut(s) 296, 431, 566
BseDI CCNNGG 1 cut(s) 906
BseGI GGATG 2 cut(s) 133, 634
BseJI GATNNNNATC 2 cut(s) 680, 893
BseLI CCNNNNNNNGG 3 cut(s) 83, 589, 625
BseMI GCAATG 1 cut(s) 969
BseMII CTCAG 5 cut(s) 726, 834, 939, 1044, 1119
BseNI ACTGG 4 cut(s) 88, 314, 444, 583
BseSI GKGCMC 1 cut(s) 641
BseXI GCAGC 6 cut(s) 733, 756, 814, 817, 946, 1102
BshFI GGCC 2 cut(s) 815, 1025
BsiHKAI GWGCWC 2 cut(s) 792, 1175
BslFI GGGAC 2 cut(s) 337, 735
BslI CCNNNNNNNGG 3 cut(s) 83, 589, 625
BsmAI GTCTC 2 cut(s) 182, 566
BsmFI GGGAC 2 cut(s) 337, 735
BsnI GGCC 2 cut(s) 815, 1025
Bso31I GGTCTC 1 cut(s) 182
Bsp1286I GDGCHC 3 cut(s) 641, 792, 1175
Bsp143I GATC 4 cut(s) 442, 492, 1122, 1159
Bsp1720I GCTNAGC 2 cut(s) 735, 1001
Bsp19I CCATGG 1 cut(s) 906
BspANI GGCC 2 cut(s) 815, 1025
BspCNI CTCAG 5 cut(s) 727, 835, 940, 1045, 1120
BspMI ACCTGC 1 cut(s) 509
BspPI GGATC 2 cut(s) 450, 1154
BspQI GCTCTTC 1 cut(s) 855
BspTI CTTAAG 1 cut(s) 10
BspTNI GGTCTC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 969
BsrI ACTGG 4 cut(s) 88, 314, 444, 583
BssECI CCNNGG 1 cut(s) 906
BssMI GATC 4 cut(s) 442, 492, 1122, 1159
BssT1I CCWWGG 1 cut(s) 906
Bst2UI CCWGG 3 cut(s) 296, 431, 566
Bst4CI ACNGT 3 cut(s) 199, 215, 560
Bst6I CTCTTC 1 cut(s) 855
BstAFI CTTAAG 1 cut(s) 10
BstAPI GCANNNNNTGC 1 cut(s) 139
BstC8I GCNNGC 1 cut(s) 476
BstDEI CTNAG 7 cut(s) 407, 735, 843, 948, 1001, 1053, 1128
BstDSI CCRYGG 1 cut(s) 906
BstEII GGTNACC 1 cut(s) 370
BstF5I GGATG 2 cut(s) 133, 634
BstKTI GATC 4 cut(s) 445, 495, 1125, 1162
BstMAI GTCTC 2 cut(s) 182, 566
BstMBI GATC 4 cut(s) 442, 492, 1122, 1159
BstMWI GCNNNNNNNGC 7 cut(s) 139, 703, 827, 839, 959, 968, 1072
BstNI CCWGG 3 cut(s) 296, 431, 566
BstPI GGTNACC 1 cut(s) 370
BstSCI CCNGG 3 cut(s) 294, 429, 564
BstSLI GKGCMC 1 cut(s) 641
BstV1I GCAGC 6 cut(s) 733, 756, 814, 817, 946, 1102
BstV2I GAAGAC 3 cut(s) 22, 534, 621
BstX2I RGATCY 2 cut(s) 442, 1159
BstYI RGATCY 2 cut(s) 442, 1159
BsuRI GGCC 2 cut(s) 815, 1025
BtgI CCRYGG 1 cut(s) 906
BtsCI GGATG 2 cut(s) 133, 634
BtsIMutI CAGTG 4 cut(s) 90, 220, 321, 639
BveI ACCTGC 1 cut(s) 509
Cac8I GCNNGC 1 cut(s) 476
CseI GACGC 2 cut(s) 308, 752
CsiI ACCWGGT 1 cut(s) 294
Csp6I GTAC 1 cut(s) 206
CviAII CATG 6 cut(s) 113, 139, 539, 811, 907, 1021
CviQI GTAC 1 cut(s) 206
DdeI CTNAG 7 cut(s) 407, 735, 843, 948, 1001, 1053, 1128
DpnI GATC 4 cut(s) 444, 494, 1124, 1161
DpnII GATC 4 cut(s) 442, 492, 1122, 1159
EaeI YGGCCR 2 cut(s) 813, 1023
Eam1104I CTCTTC 1 cut(s) 855
EarI CTCTTC 1 cut(s) 855
Ecl136II GAGCTC 2 cut(s) 790, 1173
Eco130I CCWWGG 1 cut(s) 906
Eco24I GRGCYC 2 cut(s) 792, 1175
Eco31I GGTCTC 1 cut(s) 182
Eco53kI GAGCTC 2 cut(s) 790, 1173
Eco57I CTGAAG 3 cut(s) 288, 546, 975
Eco91I GGTNACC 1 cut(s) 370
EcoICRI GAGCTC 2 cut(s) 790, 1173
EcoO65I GGTNACC 1 cut(s) 370
EcoRII CCWGG 3 cut(s) 294, 429, 564
EcoT14I CCWWGG 1 cut(s) 906
EcoT38I GRGCYC 2 cut(s) 792, 1175
ErhI CCWWGG 1 cut(s) 906
FaeI CATG 6 cut(s) 116, 142, 542, 814, 910, 1024
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FaqI GGGAC 2 cut(s) 337, 735
FatI CATG 6 cut(s) 112, 138, 538, 810, 906, 1020
FbaI TGATCA 1 cut(s) 492
Fnu4HI GCNGC 6 cut(s) 747, 770, 828, 831, 960, 1116
FokI GGATG 2 cut(s) 120, 641
FriOI GRGCYC 2 cut(s) 792, 1175
Fsp4HI GCNGC 6 cut(s) 747, 770, 828, 831, 960, 1116
FspBI CTAG 2 cut(s) 71, 605
GluI GCNGC 6 cut(s) 747, 770, 828, 831, 960, 1116
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 2 cut(s) 815, 1025
HgaI GACGC 2 cut(s) 308, 752
Hin1II CATG 6 cut(s) 116, 142, 542, 814, 910, 1024
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 1064
HinfI GANTC 1 cut(s) 278
HphI GGTGA 3 cut(s) 16, 273, 623
Hpy166II GTNNAC 4 cut(s) 90, 301, 808, 1018
Hpy188I TCNGA 8 cut(s) 367, 421, 688, 949, 1122, 1129, 1154, 1164
Hpy188III TCNNGA 4 cut(s) 496, 898, 1087, 1176
Hpy8I GTNNAC 4 cut(s) 90, 301, 808, 1018
Hpy99I CGWCG 1 cut(s) 324
HpyAV CCTTC 8 cut(s) 352, 454, 602, 712, 841, 973, 1051, 1159
HpyCH4III ACNGT 3 cut(s) 199, 215, 560
HpyCH4V TGCA 8 cut(s) 50, 116, 133, 727, 749, 940, 962, 987
HpyF10VI GCNNNNNNNGC 7 cut(s) 139, 703, 827, 839, 959, 968, 1072
HpyF3I CTNAG 7 cut(s) 407, 735, 843, 948, 1001, 1053, 1128
Hsp92II CATG 6 cut(s) 116, 142, 542, 814, 910, 1024
Ksp22I TGATCA 1 cut(s) 492
Kzo9I GATC 4 cut(s) 442, 492, 1122, 1159
LguI GCTCTTC 1 cut(s) 855
LmnI GCTCC 3 cut(s) 79, 169, 453
Lsp1109I GCAGC 6 cut(s) 733, 756, 814, 817, 946, 1102
LweI GCATC 1 cut(s) 142
MabI ACCWGGT 1 cut(s) 294
MaeI CTAG 2 cut(s) 71, 605
MaeIII GTNAC 2 cut(s) 279, 370
MalI GATC 4 cut(s) 444, 494, 1124, 1161
MboI GATC 4 cut(s) 442, 492, 1122, 1159
MflI RGATCY 2 cut(s) 442, 1159
MhlI GDGCHC 3 cut(s) 641, 792, 1175
MlsI TGGCCA 1 cut(s) 815
MluCI AATT 2 cut(s) 102, 289
MluNI TGGCCA 1 cut(s) 815
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 531
Mox20I TGGCCA 1 cut(s) 815
MscI TGGCCA 1 cut(s) 815
MseI TTAA 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 815
MspCI CTTAAG 1 cut(s) 10
MspR9I CCNGG 3 cut(s) 296, 431, 566
MvaI CCWGG 3 cut(s) 296, 431, 566
MwoI GCNNNNNNNGC 7 cut(s) 139, 703, 827, 839, 959, 968, 1072
NcoI CCATGG 1 cut(s) 906
NdeII GATC 4 cut(s) 442, 492, 1122, 1159
NlaIII CATG 6 cut(s) 116, 142, 542, 814, 910, 1024
NmuCI GTSAC 1 cut(s) 279
PciSI GCTCTTC 1 cut(s) 855
PflMI CCANNNNNTGG 3 cut(s) 83, 589, 625
PfoI TCCNGGA 1 cut(s) 564
PkrI GCNGC 6 cut(s) 748, 771, 829, 832, 961, 1117
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
Psp124BI GAGCTC 2 cut(s) 792, 1175
Psp6I CCWGG 3 cut(s) 294, 429, 564
PspEI GGTNACC 1 cut(s) 370
PspGI CCWGG 3 cut(s) 294, 429, 564
PsuI RGATCY 2 cut(s) 442, 1159
RsaI GTAC 1 cut(s) 207
RsaNI GTAC 1 cut(s) 206
SacI GAGCTC 2 cut(s) 792, 1175
SapI GCTCTTC 1 cut(s) 855
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 6 cut(s) 747, 770, 828, 831, 960, 1116
Sau3AI GATC 4 cut(s) 442, 492, 1122, 1159
ScaI AGTACT 1 cut(s) 207
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 3 cut(s) 296, 431, 566
SduI GDGCHC 3 cut(s) 641, 792, 1175
SexAI ACCWGGT 1 cut(s) 294
SfaNI GCATC 1 cut(s) 142
SmlI CTYRAG 4 cut(s) 10, 28, 386, 822
SmoI CTYRAG 4 cut(s) 10, 28, 386, 822
Sse9I AATT 2 cut(s) 102, 289
SspI AATATT 1 cut(s) 598
SspMI CTAG 2 cut(s) 71, 605
SstI GAGCTC 2 cut(s) 792, 1175
StyD4I CCNGG 3 cut(s) 294, 429, 564
StyI CCWWGG 1 cut(s) 906
TaaI ACNGT 3 cut(s) 199, 215, 560
TaqI TCGA 4 cut(s) 276, 322, 792, 1086
TasI AATT 2 cut(s) 102, 289
TatI WGTACW 1 cut(s) 205
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TscAI CASTG 4 cut(s) 97, 220, 321, 646
TseFI GTSAC 1 cut(s) 279
TseI GCWGC 6 cut(s) 746, 769, 827, 830, 959, 1115
Tsp45I GTSAC 1 cut(s) 279
TspDTI ATGAA 8 cut(s) 168, 174, 266, 396, 672, 885, 893, 1095
TspRI CASTG 4 cut(s) 97, 220, 321, 646
Van91I CCANNNNNTGG 3 cut(s) 83, 589, 625
Vha464I CTTAAG 1 cut(s) 10
XcmI CCANNNNNNNNNTGG 1 cut(s) 321
XspI CTAG 2 cut(s) 71, 605
ZrmI AGTACT 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.