RLG00000013304

Mediates both low-affinity uptake and efflux of sugar across the membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017984
Physical Location & Seq
Reverse (-)
29475977 .. 29476999
1023 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013304

Sequence Viewer

Length: 393 bp
ATGGCCTATGAAACTCCCTGTGATAGTCATGGCTCCAGTACTGTGGTCGATCGATCAACAACCATGAATATTCCCAAGGCCATGTTCAGACTAGTGTTTGGAAATGTCATCGCTATGTTCTTCATCTTGTGGTCGCCAATGAGTAAAGTAAAGCAAATCATAAAGAACAAGTTTACAGAGCAGCTCTCAAGCATTTCGTGCCAGATCACTTTTTTCAAGCGCCTCCTTTCTGCTTGGTTTTGCTTGCCCTATGTGTCACCAAACGACATTTTCATATCAATCATCAATGGCATTGGTGCAGCTATTGAAGCCATGAACCTGTTCATCCTCGTGATGTTTGCCCTGAAGAGTGAGAATGAACAACTTCTTGAATTGCTCGCCTTTGTATTCTAA

Protein Analysis

131

Amino Acids

14.73

Weight (kDa)

7.7

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MtN3_slv PF03083 37 - 116 2e-07 Sugar efflux transporter for intercellular exchange
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017984)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g14850
malus_domestica MD05G1012300.v1.1
prunus_persica Prupe.8G017500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0277421
rosa_laevigata RLG00000013304
rosa_roxburghii Rroxscaffold_7G00191350
rosa_samantha Rh6AG218800 Rh6BG222700 Rh6CG225300 Rh6DG215600
rosa_wichuraiana Rw6G019170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 365
AfaI GTAC 1 cut(s) 40
AgsI TTSAA 3 cut(s) 217, 308, 371
AhlI ACTAGT 1 cut(s) 91
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AluBI AGCT 2 cut(s) 184, 302
AluI AGCT 2 cut(s) 184, 302
AoxI GGCC 2 cut(s) 3, 78
ApeKI GCWGC 2 cut(s) 181, 299
Asp700I GAANNNNTTC 2 cut(s) 320, 363
AspLEI GCGC 1 cut(s) 222
AsuHPI GGTGA 1 cut(s) 249
BauI CACGAG 1 cut(s) 329
BbvI GCAGC 2 cut(s) 193, 311
BcuI ACTAGT 1 cut(s) 91
BfaI CTAG 1 cut(s) 92
BfoI RGCGCY 1 cut(s) 223
BisI GCNGC 2 cut(s) 182, 300
BlsI GCNGC 2 cut(s) 183, 301
BmcAI AGTACT 1 cut(s) 40
BmiI GGNNCC 1 cut(s) 34
BplI GAGNNNNNCTC 2 cut(s) 170, 202
BpmI CTGGAG 1 cut(s) 19
BpuEI CTTGAG 1 cut(s) 172
Bsa29I ATCGAT 1 cut(s) 52
BsaJI CCNNGG 1 cut(s) 75
Bse1I ACTGG 1 cut(s) 36
BseCI ATCGAT 1 cut(s) 52
BseDI CCNNGG 1 cut(s) 75
BseGI GGATG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 36
BseXI GCAGC 2 cut(s) 193, 311
BsgI GTGCAG 1 cut(s) 318
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 2 cut(s) 5, 80
BshVI ATCGAT 1 cut(s) 52
BsiEI CGRYCG 1 cut(s) 52
BsnI GGCC 2 cut(s) 5, 80
Bsp143I GATC 3 cut(s) 49, 53, 204
BspANI GGCC 2 cut(s) 5, 80
BspDI ATCGAT 1 cut(s) 52
BspLI GGNNCC 1 cut(s) 34
BsrI ACTGG 1 cut(s) 36
BssECI CCNNGG 1 cut(s) 75
BssMI GATC 3 cut(s) 49, 53, 204
BssSI CACGAG 1 cut(s) 329
BssT1I CCWWGG 1 cut(s) 75
Bst2BI CACGAG 1 cut(s) 329
Bst4CI ACNGT 1 cut(s) 43
Bst6I CTCTTC 1 cut(s) 341
BstAPI GCANNNNNTGC 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 245, 378
BstF5I GGATG 1 cut(s) 324
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 3 cut(s) 52, 56, 207
BstMBI GATC 3 cut(s) 49, 53, 204
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 2 cut(s) 198, 308
BstV1I GCAGC 2 cut(s) 193, 311
BstXI CCANNNNNNTGG 1 cut(s) 43
Bsu15I ATCGAT 1 cut(s) 52
BsuRI GGCC 2 cut(s) 5, 80
BsuTUI ATCGAT 1 cut(s) 52
BtgZI GCGATG 1 cut(s) 94
BtsCI GGATG 1 cut(s) 324
Cac8I GCNNGC 2 cut(s) 245, 378
CfoI GCGC 1 cut(s) 222
ClaI ATCGAT 1 cut(s) 52
Csp6I GTAC 1 cut(s) 39
CviAII CATG 4 cut(s) 29, 64, 82, 313
CviJI RGCY 6 cut(s) 5, 33, 80, 184, 302, 311
CviKI_1 RGCY 6 cut(s) 5, 33, 80, 184, 302, 311
CviQI GTAC 1 cut(s) 39
DpnI GATC 3 cut(s) 51, 55, 206
DpnII GATC 3 cut(s) 49, 53, 204
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
Eco130I CCWWGG 1 cut(s) 75
Eco57I CTGAAG 1 cut(s) 365
EcoT14I CCWWGG 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 4 cut(s) 32, 67, 85, 316
FaiI YATR 9 cut(s) 9, 30, 65, 83, 116, 161, 252, 275, 314
FatI CATG 4 cut(s) 28, 63, 81, 312
Fnu4HI GCNGC 2 cut(s) 182, 300
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 2 cut(s) 182, 300
FspBI CTAG 1 cut(s) 92
GlaI GCGC 1 cut(s) 221
GluI GCNGC 2 cut(s) 182, 300
GsuI CTGGAG 1 cut(s) 19
HaeII RGCGCY 1 cut(s) 223
HaeIII GGCC 2 cut(s) 5, 80
HhaI GCGC 1 cut(s) 222
Hin1II CATG 4 cut(s) 32, 67, 85, 316
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HphI GGTGA 1 cut(s) 249
Hpy166II GTNNAC 1 cut(s) 174
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 2 cut(s) 331, 368
Hpy8I GTNNAC 1 cut(s) 174
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4V TGCA 1 cut(s) 299
HpyF10VI GCNNNNNNNGC 2 cut(s) 198, 308
Hsp92II CATG 4 cut(s) 32, 67, 85, 316
HspAI GCGC 1 cut(s) 220
Kzo9I GATC 3 cut(s) 49, 53, 204
LmnI GCTCC 1 cut(s) 38
LpnPI CCDG 5 cut(s) 31, 49, 215, 332, 356
Lsp1109I GCAGC 2 cut(s) 193, 311
MaeI CTAG 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 255
MalI GATC 3 cut(s) 51, 55, 206
MboI GATC 3 cut(s) 49, 53, 204
MboII GAAGA 2 cut(s) 112, 358
MluCI AATT 1 cut(s) 371
MnlI CCTC 2 cut(s) 233, 338
MroXI GAANNNNTTC 2 cut(s) 320, 363
MslI CAYNNNNRTG 2 cut(s) 113, 329
MwoI GCNNNNNNNGC 2 cut(s) 198, 308
NdeII GATC 3 cut(s) 49, 53, 204
NlaIII CATG 4 cut(s) 32, 67, 85, 316
NlaIV GGNNCC 1 cut(s) 34
NmuCI GTSAC 1 cut(s) 255
PdmI GAANNNNTTC 2 cut(s) 320, 363
PkrI GCNGC 2 cut(s) 183, 301
Ple19I CGATCG 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 34
PvuI CGATCG 1 cut(s) 52
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
RseI CAYNNNNRTG 2 cut(s) 113, 329
SatI GCNGC 2 cut(s) 182, 300
Sau3AI GATC 3 cut(s) 49, 53, 204
ScaI AGTACT 1 cut(s) 40
SetI ASST 3 cut(s) 186, 304, 321
SmiMI CAYNNNNRTG 2 cut(s) 113, 329
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
SpeI ACTAGT 1 cut(s) 91
Sse9I AATT 1 cut(s) 371
SspI AATATT 1 cut(s) 70
SspMI CTAG 1 cut(s) 92
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 1 cut(s) 43
TaqI TCGA 2 cut(s) 48, 52
TasI AATT 1 cut(s) 371
TatI WGTACW 1 cut(s) 38
TseFI GTSAC 1 cut(s) 255
TseI GCWGC 2 cut(s) 181, 299
Tsp45I GTSAC 1 cut(s) 255
TspDTI ATGAA 7 cut(s) 24, 80, 112, 262, 313, 329, 372
XmnI GAANNNNTTC 2 cut(s) 320, 363
XspI CTAG 1 cut(s) 92
ZrmI AGTACT 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.